Scanning Electron Microscopic Study of the White Pulp of Spleen in Adult Goats

Lymphoid Cells ◽

White Pulp ◽

Electron Microscopic ◽

Reticular Cells ◽

Structural Connections ◽

Marginal Zones ◽

The white pulp of the spleen in adult goats was thoroughly screened under the scanning electron microscope (VEGA3 TESCAN). The study revealed the presence of lymphoid and non-lymphoid cells. The T lymphocytes and B lymphocytes could morphologically be differentiated under the magnification of 8000 to 20000 times by Scanning electron microscopy. The changes in number of cells in relation to the age was also recorded. The peri arterial lymphatic sheath and its structural connections with Reticular cells and other non-lymphoid cells were clearly demonstrated. The types of lymphoid cells and their arrangement around the central arteries upto the marginal zones were observed in detail. A conclusion about the type of circulation in the spleen of goats was arrived after three dimentional observation of the tissue under scanning electron microscope.

Scanning Electron Microscopic Study of the Eggs of the Warthog Louse (Haematopinus Phacochoeri)

Microscopy and Microanalysis ◽

10.1017/s1431927600007455 ◽

1997 ◽

Vol 3 (S2) ◽

pp. 113-114

Author(s):

C. Baker ◽

E.D. Green

Keyword(s):

Electron Microscope ◽

Environmental Changes ◽

General Structure ◽

Electron Microscopic ◽

Razor Blade ◽

Sem Study ◽

The large eggs of Haematopinus phacochoeri are found attached to the long dorsal mane bristles of the diurnal warthog. The eggs are generally exposed to great environmental stresses such as excessive sunlight and temperature as well as the occasional plunge under water or mud during grooming. This investigation was therefore undertaken to determine how the eggs of H. phococoeri are adapted to tolerate the various environmental changes it is constantly exposed to. To our knowledge no other SEM study on the eggs of this species has been performed.Specimens still attached to the bristles were fixed in 70% ethanol, dehydrated and ultrasonically cleaned. Some eggs were longitudinally sectioned with a razor blade to expose the chorionic layers. The eggs were critical point dried in liquid CO2, mounted and viewed by means of a Leica Stereoscan 420 scanning electron microscope.The general structure of the shell consists of two layers of chorion, i.e. the endo-and exochorion. These two layers are attached at the pre-formed line of weakness at the operculum and again near the hydropyle. Elsewhere these two layers are completely separated by the respiratory layer.

Additional taxonomic studies on Avena fatuoids: some morphological attributes seen using the scanning electron microscope

Canadian Journal of Botany ◽

10.1139/b71-101 ◽

1971 ◽

Vol 49 (5) ◽

pp. 647-649 ◽

Cited By ~ 4

Author(s):

Bernard R. Baum

Keyword(s):

Electron Microscope ◽

Avena Fatua ◽

The Other ◽

Electron Microscopic ◽

Morphological Attributes ◽

Taxonomic Studies ◽

This paper describes and evaluates the results of a scanning electron microscopic study of the sculpture of the surfaces of caryopses, glumes, lemmas, awn columns, and awn subules of Avena fatua, normal cultivated oats (A. sativa), fatuoids, and F1 hybrids A. fatua × sativa. Only qualitative differences were sought, and none were detected in the glumes and on the caryopses. The prickles on the lemmas, on the awn columns, and on the awn subules all appear in two character states. In the case of the lemmas one state is found in the normal cultivated oats, fatuoids, and F1 hybrids, and the other in A. fatua. On the other hand in the awn subules one state is found in the normal cultivated oats and the other in fatuoids, F1 hybrids, and A. fatua, while in the awn columns one state occurs in cultivated oats and the other in A. fatua, but in the F1 hybrids and fatuoids both states occur. These findings provide taxonomic evidence additional to that described earlier based on lodicules coupled with traditional characters.

Surface Morphological Changes in Human Enamel Following Bleaching: An in vitro Scanning Electron Microscopic Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1159 ◽

2012 ◽

Vol 13 (3) ◽

pp. 405-415 ◽

Cited By ~ 3

Author(s):

LM Ranganath ◽

B Sunil Rao ◽

AG Rajesh ◽

KS Prem Kumar

Keyword(s):

Electron Microscope ◽

Morphological Changes ◽

Carbamide Peroxide ◽

Electron Microscopic ◽

Human Enamel ◽

ABSTRACT Objective The purpose of this study was to evaluate, using scanning electron microscopy (SEM), the morphological and structural changes of the enamel induced by three bleaching agents namely old McInnes solution, modified McInnes solution and 10% carbamide peroxide gel at different time intervals. Materials and methods Fifteen freshly extracted noncarious human central incisors with intact enamel surface were selected. The teeth were sectioned at the cementoenamel junction separating the crown portion from the root using a diamond separating disk. Following this, the samples were subjected to three different bleaching agents: Group 1: Old McInnes solution, group 2: modified McInnes solution and group 3: 10% carbamide peroxide for a period of 15, 30 and 60 minutes, 24 and 30 hours time interval. The sample stubs were subjected to scanning electron microscope and were photographed at 2000 and 10,000 magnifications. Conclusion The present study revealed no indication of either etching or significant change in surface morphology of enamel when evaluated under scanning electron microscope after 6 weeks treatment with various bleaching agents. Clinical significance Morphological alterations in bleached enamel are both concentration and time dependent. How to cite this article Rajesh AG, Ranganath LM, Kumar KSP, Rao BS. Surface Morphological Changes in Human Enamel Following Bleaching: An in vitro Scanning Electron Microscopic Study. J Contemp Dent Pract 2012;13(3):405-415.

A scanning electron microscopic study of epicuticular waxes of glumesin Avena magna, A. murphyi, and A. sterilis

Canadian Journal of Botany ◽

10.1139/b73-305 ◽

1973 ◽

Vol 51 (12) ◽

pp. 2381-2383 ◽

Cited By ~ 3

Author(s):

B. R. Baum ◽

V. E. Hadland

Keyword(s):

Electron Microscope ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Epicuticular Waxes ◽

Electron Microscopic ◽

Avena Species ◽

The ultrastructure of epicuticular glume waxes in two tetraploid Avena species, A. magna and A. murphyi, and in one hexaploid species, A. sterilis, has been studied and documented with the aid of the scanning electron microscope (SEM). The usefulness of this approach for taxonomy and diagnostic purposes has been evaluated, and the specific configurations of those epicuticular waxes compared in relation to the genomes.

A Light and Scanning Electron Microscopic Study of Enamel Decalcification in Children Living in a Water-fluoridated Area

Journal of Dental Research ◽

10.1177/00220345900690100101 ◽

1990 ◽

Vol 69 (10) ◽

pp. 1626-1633 ◽

Cited By ~ 23

Author(s):

A. Thylstrup ◽

R.M. Boyar ◽

L. Holmen ◽

G.H. Bowden

Keyword(s):

Electron Microscope ◽

Dental Fluorosis ◽

Electron Microscopic ◽

Crystal Dissolution ◽

Time Periods ◽

Short Time ◽

Eleven children, each having one or two pairs of premolars to be extracted for orthodontic purposes, participated in the study. The model involved placement of a special orthodontic band that allowed the accumulation of plaque in a defined area between the band and the buccal enamel. Examination of enamel changes was carried out in experimental teeth that had been exposed to local plaque accumulation for one, two, four, eight, or 14 days. The specimens were examined under the light (LM) and the scanning electron microscope (SEM). All teeth had signs of very mild dental fluorosis. No indications of demineralization were noted after one day. SEM examination showed signs of crystal dissolution in some of the two-day specimens. Six of eight four-day specimens exhibited surface dissolution. All eight- and 14-day specimens showed signs of surface demineralization in the LM as well as in the SEM. These observations documented that undisturbed bacterial deposits are capable of initiating enamel demineralization within short time periods, even in children living in a water-fluoridated area.

The epicuticular waxes of glumes of Avena: a scanning electron microscopic study of the morphological patterns in all the species

Canadian Journal of Botany ◽

10.1139/b75-200 ◽

1975 ◽

Vol 53 (16) ◽

pp. 1712-1718 ◽

Cited By ~ 9

Author(s):

B. R. Baum ◽

V. E. Hadland

Keyword(s):

Electron Microscope ◽

The Other ◽

Epicuticular Waxes ◽

Electron Microscopic ◽

Special Cases ◽

Scanning Electron ◽

Morphological Patterns

The epicuticular waxes of glumes of 357 samples, representing all the 27 species of the genus Avena. were examined under the scanning electron microscope. Three basic patterns were discerned, plates, knobs, and filaments, and each sample has been scored for the types found. A fourth category of unstructural pattern has also been scored. Variability within patterns was observed but not scored. It is concluded that epicuticular waxes on glumes in Avena cannot be used for identification of specimens to species except for various special cases when the choice to be made is between two or three alternatives. The main finding is that the degree of variability of the patterns is inversely proportional to the ploidy level. The hexaploid species consist almost exclusively of filaments: the other species have all the three basic patterns, with some species possessing a high proportion of only two patterns.

A scanning electron microscopic study on dissolving process of cholesterol gallstones by chenodeoxycholic acid (CDCA)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083485 ◽

1978 ◽

Vol 36 (2) ◽

pp. 522-523

Author(s):

T. Kanetaka ◽

M. Cho ◽

S. Kawamura ◽

T. Sado ◽

K. Hara

Keyword(s):

Electron Microscope ◽

Chenodeoxycholic Acid ◽

Outer Surface ◽

Electron Microscopic ◽

Cholesterol Gallstones ◽

The Core ◽

Acceleration Voltage ◽

The authors have investigated the dissolution process of human cholesterol gallstones using a scanning electron microscope(SEM). This study was carried out by comparing control gallstones incubated in beagle bile with gallstones obtained from patients who were treated with chenodeoxycholic acid(CDCA).The cholesterol gallstones for this study were obtained from 14 patients. Three control patients were treated without CDCA and eleven patients were treated with CDCA 300-600 mg/day for periods ranging from four to twenty five months. It was confirmed through chemical analysis that these gallstones contained more than 80% cholesterol in both the outer surface and the core.The specimen were obtained from the outer surface and the core of the gallstones. Each specimen was attached to alminum sheet and coated with carbon to 100Å thickness. The SEM observation was made by Hitachi S-550 with 20 kV acceleration voltage and with 60-20, 000X magnification.

Human Stapes Crura: Normal Ultrastructure. Scanning Electron Microscopic Findings

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100116267 ◽

1975 ◽

Vol 33 ◽

pp. 528-529

Author(s):

M.D. Graham

Keyword(s):

Electron Microscope ◽

Surgical Treatment ◽

Osmic Acid ◽

Human Cadaver ◽

Electron Microscopic ◽

Normal Human ◽

Microscopic Findings ◽

The recent development of the scanning electron microscope has added great impetus to the study of ultrastructural details of normal human ossicles. A thorough description of the ultrastructure of the human ossicles is required in order to determine changes associated with disease processes following medical or surgical treatment.Human stapes crura were obtained at the time of surgery for clinical otosclerosis and from human cadaver material. The specimens to be examined by the scanning electron microscope were fixed immediately in the operating room in a cold phosphate buffered 2% gluteraldehyde solution, washed with Ringers, post fixed in cold 1% osmic acid and dehydrated in graded alcohol. Specimens were transferred from alcohol to a series of increasing concentrations of ethyl alcohol and amyl acetate. The tissue was then critical point dried, secured to aluminum stubs and coated with gold, approximately 150A thick on a rotating stage in a vacuum evaporator. The specimens were then studied with the Kent-Cambridge S4-10 Scanning Electron Microscope at an accelerating voltage of 20KV.

Features of the architectonics of the microstructure of the primary remex of Owls (Strigiformes) due to the specifics of the flight

Proceedings of the National Academy of Sciences of Belarus Biological Series ◽

10.29235/1029-8940-2021-66-2-232-246 ◽

2021 ◽

Vol 66 (2) ◽

pp. 232-246

Author(s):

E. O. Fadeeva

Keyword(s):

Electron Microscope ◽

Cross Section ◽

Dorsal Surface ◽

Structural Features ◽

Electron Microscopic Investigation ◽

Original Research ◽

Electron Microscopic ◽

Microstructural Characteristics ◽

Conducted electron microscopic investigation of the primary remex fine structure of thirteen species of Owls (Strigiformes), using a scanning electron microscope (SEM). It is shown that Owls (Strigiformes) have a number of specific primary remex microstructural characteristics. First of all, these are the features of the structure of the pennaceous barb: a cross section configuration, a pith architectonics on the cross section and longitudinal sections, a cuticular structur of the barb. A number of the unique features in the microstructure of the vanules of the pennaceous barb have been found for the first time (at the scanning electron microscope level, at a large SEM magnification). First of all, these are the structural features of the distal barbules and the structure of the apical portion of the barb with the elongated proximal barbules and the distal barbules tightly contiguous to the ramus and closed with each other. Mentioned characteristics make for the thick velvet-like dorsal surface of the vane and the presence of a complex of peculiar “bunches” (fringes) forming the cleft edge (a fringed edge) of the inner vane – exceptionally specific adaptive characteristics in Strigiformes. Рresentenced original research results suggest that Owls (Strigiformes) have a number specific microstructural characteristics of the primary remex and also a number of the unique features in the microstructure of the primary remex which reflecting the ecological and morphological adaptations conditioned by the flight specificity.

Scanning electron microscope examination of the dental enameloid of the Cretaceous durophagous shark Ptychodus supports neoselachian classification

Journal of Paleontology ◽

10.1017/jpa.2016.64 ◽

2016 ◽

Vol 90 (4) ◽

pp. 741-762 ◽

Cited By ~ 8

Author(s):

Brian L. Hoffman ◽

Scott A. Hageman ◽

Gregory D. Claycomb

Keyword(s):

Electron Microscope ◽

Electron Microscopic Examination ◽

Middle Layer ◽

Microscope Examination ◽

Electron Microscopic ◽

Marine Deposits ◽

Greenhorn Formation ◽

AbstractAlthough Ptychodus teeth are well known in Late Cretaceous marine deposits in North America and Europe and a few specimens with jaw elements have been discovered, the taxonomic position of the shark genus Ptychodus is enigmatic due to the lack of preservation of diagnostic material other than teeth. These sharks possessed a pavement dentition suited to a diet of hard-shelled macroinvertebrates (durophagy), leading several studies to variously describe Ptychodus as a batoid, a hybodont shark, or a selachimorph. Members of the Selachimorpha share one dental synapomorphy, a triple-layered enameloid (TLE) consisting of an outer shiny-layered enameloid (SLE) of randomly oriented hydroxyapatite crystallites, a middle layer of parallel-bundled enameloid (PBE), and an inner layer of tangled-bundled enameloid (TBE). Batoids and hybodonts both have teeth with single crystallite enameloid (SCE). We examined teeth from Ptychodus collected from the Lincoln Limestone of the Greenhorn Formation of Barton County, Kansas, and compared their enameloid ultrastructure with that of a Carboniferous hybodontiform and the Cretaceous lamniform shark Squalicorax curvatus Williston, 1900. Scanning electron microscopic examination of Ptychodus shows that crystallite bundling in the form of a TLE is evident in these teeth. The PBE is most apparent at transverse enameloid ridges of Ptychodus teeth. Columns of dentine penetrate into the tooth enameloid, and the crystallites near the dentine are randomly oriented. These observations bolster the argument that Ptychodus is a genus of highly specialized selachimorph shark, rather than a hybodont or batoid.