Evaluation of Genetic Diversity in Goats of Telangana and Andhra Pradesh States of India

2021 ◽  
Author(s):  
N.K. Verma ◽  
Rekha Sharma ◽  
R.A.K. Aggarwal ◽  
P.S. Dangi
Keyword(s):  
Genetic Diversity ◽  
Andhra Pradesh ◽  
Genetic Bottleneck ◽  
Assignment Test ◽  
Inbreeding Coefficients ◽  
Bottleneck Analysis ◽  
Goat Population ◽  
The Mean ◽  
Very High ◽  
F Statistics

Background: The goat population of Andhra Pradesh and Telangana states is about five million, respectively. The goats of these two states lack phenotypic uniformity. But it is not known whether these populations have any genetically uniform group that can be registered as a breed. The study was undertaken to explore possibility of any potential new goat germplasm.Methods: Study was carried out at ICAR-NBAGR during 2017-19. Genetic diversity and differentiation was evaluated by using 22 microsatellite markers in three goat populations: Telangana Black (TB, n=26), Telangana Mixed (TM, n=49) and one Andhra Pradesh goat population (AP, n=45). Their genetic differentiation was compared with that of geographically closely distributed registered breeds viz. Bidri (n=28) and Nandidurga (n=48) of Karnatka and Ganjam (n=48) of Odisha. Result: The mean allele frequency observed was 6.59 (TB), 7.27 (TM) and 8.36 (AP). Expected number of alleles varied from 3.33 in (TB) to 3.69 in AP goats. Observed heterozygosity was lowest in the TM (0.474) followed by TB (0.504) and was highest in the AP goats (0.569). AP goat population had 6.3% heterozygote deficiency, whereas, both TB (15.4%) and TM (17.5%) had very high inbreeding coefficients. A total of 344 alleles were detected across the 22 loci in six goat groups. F-statistics, the pair-wise Nei’s genetic distance, assignment test and Baysian approach suggested that AP goats are distinct from two Telangana goat populations as well as from the other geographically closely related registered goat breeds. Genetic bottleneck analysis indicated the absence of any detectably large, recent genetic bottleneck in AP population. Altogether, the study identified Andhra Pradesh (AP) goats to be a new potential goat germplasm of India.

scholarly journals Genetic diversity of Avena strigosa Schreb. ecotypes on the basis of isoenzyme markers

2009 ◽  
Vol 15 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Katarzyna Kubiak
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Avena Strigosa ◽  
Total Genetic Diversity ◽  
Geographical Regions ◽  
The World ◽  
Total Variability ◽  
The Mean ◽  
Intrapopulation Diversity ◽  
Very High

Genetic diversity ofAvena strigosaSchreb. ecotypes on the basis of isoenzyme markersGenetic diversity was analyzed in 19 ecotypes of the diploid oatA. strigosaoriginating from various geographical regions of the world. Six isoenzyme systems (AAT, ACP, EST, LAP, MDH, PX) were studied and 16 loci were identified. Only two loci (Est4andMdh2) were polymorphic. Ecotypes were characterized by the percentage of polymorphic loci (P=3.3%), the mean number of alleles per locus (A=1.04) and intrapopulation diversity (HS=0.013). Total genetic diversity (HT=0.07) and interpopulation diversity (DST=0.057) were examined as well. The value of the coefficient of gene differentiation (GST=0.821) indicated that diversity among populations was an important contributor to total variability. Genetic similarity betweenA. strigosapopulations was very high (IN=0.94). Cluster analysis did not demonstrate strongly differentiated groups among the ecotypes examined.

scholarly journals Is it possible to obtain zero estimates of genetic diversity? A case study of the Nigerian indigeneous goat breeds at the β-lactoglobulin gene locus

2017 ◽  
Vol 33 (4) ◽  
pp. 375-388
Author(s):  
Emeka Ezewudo ◽  
Geka Abubakar ◽  
Sunday Egena ◽  
Olushola Alabi
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Gene Locus ◽  
Polymorphic Locus ◽  
Information Index ◽  
Expected Heterozygosity ◽  
Goat Population ◽  
The Mean ◽  
Β Lactoglobulin

The current investigation was conducted to appraise the genetic diversity and genetic distance of three goat populations namely; Red Sokoto, Sahel and West African Dwarf (WAD), in Nigeria, making use of blood samples collected from 20, 20 and 20 individual from which blood DNAs were extraction, respectively. The DNAs extracted were used to study polymorphism at the ?-lactoglobulin gene locus using RLFP-PCR process. Results revealed that the mean total number of alleles was 1 while the effective number of alleles was also 1. The percentage of polymorphic locus was 0% while Shannon?s information index, observed homozygousity, expected heterozygosity, unbiased expected heterozygosity and inbreeding coefficient (F) were all observed to be 0.000. The pairwise Fst was 0.000 between all the breeds of goats. Variation within and between the populations of goats was 0% at p>0.05. The genetic distance between the goat breeds was 0.000. The present study revealed that RLFP-PCR may not be a powerful tool for the study of the ?-lactoglobulin gene locus and hence other methodologies should be employed for a broader judgment on the genetic status of the goat population at the locus.

AFLP markers for analysis of genetic diversity and structure of teak (Tectona grandis) in India

2015 ◽  
Vol 45 (3) ◽  
pp. 297-306 ◽  
Author(s):  
Vivek Vaishnaw ◽  
Naseer Mohammad ◽  
Syed Arif Wali ◽  
Randhir Kumar ◽  
Shashi Bhushan Tripathi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Central India ◽  
Tectona Grandis ◽  
Neighbor Joining ◽  
Peninsular India ◽  
Genetic Diversity And Structure ◽  
Superior Genotypes ◽  
Very High ◽  
Elite Trees ◽  
F Statistics

Five amplified fragment length polymorphism (AFLP) primer combinations (E-AAC × M-CAT, E-AAC × M-CTG, E-ACA × M-CTC, E-ACA × M-CTA, and E-ACC × M-CTA) were employed for analysis of genetic diversity, differentiation, and structure of 96 genotypes of teak (Tectona grandis L. f.) from 10 natural locations in India. The analysis of the AFLP marker data by both versions, i.e., GST and θ, of F statistics showed a similar trend due to the outcrossing nature of teak. The primer combination E-AAC × M-CAT detected maximum polymorphism in the teak genome. The analysis of molecular variance assigned a large proportion of the genetic diversity to within sampled locations and a very small proportion to among locations. The population genetic structure resolved by the neighbor joining tree, principal coordinate analysis, and no-admixture and admixture model Bayesian-based analyses irrefutably revealed two distinct centers of teak diversity, i.e., central India and peninsular India. Furthermore, the very high proportion of genetic diversity residing within locations encourages the intensive selection and (or) collection of diverse superior genotypes (elite trees) from each location for the conservation of germplasm and the genetic improvement of teak.

scholarly journals Genetic diversity and relationship between wild and cultivated cowpea [Vigna unguiculata (L.) Walp.] as assessed by allozyme markers

2021 ◽  
Vol 54 (1) ◽  
pp. 201-208
Author(s):  
Eric Bertrand Kouam ◽  
Geoffrey Mwanza Muluvi ◽  
Rémy Stéphane Pasquet
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Bottleneck ◽  
Wild Plant ◽  
Cultivated Plants ◽  
Upgma Dendrogram ◽  
Traditional Agroecosystems ◽  
Isozyme Loci ◽  
The Mean ◽  
The Relationship

Abstract In Cameroon, cowpea plays an important role in traditional agroecosystems. Genetic variation in wild and cultivated cowpea in Cameroon has not yet been documented. Allozyme markers because of their codominance and polymorphism are useful tools for studying genetic variation and disparity in plant species. The present study was undertaken to elucidate the relationship between wild and cultivated cowpea from Cameroon. Ten enzyme systems encoding nineteen isozyme loci were used on 62 cowpea germplasm (45 wild and 17 cultivated). A total of thirty-two alleles were found. One allele was only found in cultivated samples (Enp98 ). Eight alleles were specific only to wild plant (Amp2 98 , Amp3a 103 , Amp4 96 , Fdh104 , Idh2 95 , Pgi3 92 , Pgm2 95 and Sdh95 ). Twenty-three alleles were common to both wild and cultivated accessions. Amp2 10 2 (z = −4.633, p < 0.001) and Fle3 96 (z = −2.858, p < 0.010) were significantly more represented in cultivated compared to wild cowpea forms. The mean number of alleles per locus in wild (1.632 alleles/locus) cowpea were significantly higher (t = 2.805, p < 0.010) compared to cultivated (1.263 alleles/locus) cowpea. Also, the proportion of polymorphic loci (P = 52.63%) and average Nei’s genetic diversity (He = 0.126) were important in wild, compared to the cultivated plants: P = 26.31% and He = 0.063, respectively. The low level of diversity found in domesticated accessions compared to wild can be attributed to a major genetic bottleneck that probably happened during the domestication process. Cluster analysis revealed by UPGMA dendrogram separated the 62 accessions into three clusters. Although an admixture of both wild and cultivated accessions within the same cluster were found, the dendogram, however, highlighted a visible separation between wild and cultivated cowpea. Wild cowpea with many more private alleles indicates an untouched resource available for future breeding.

scholarly journals Genetic diversity of Brazilian Brahman cattle by pedigree analysis

2018 ◽  
Vol 53 (1) ◽  
pp. 74-79 ◽  
Author(s):  
Ligia Cavani ◽  
Rafael Medeiros de Oliveira Silva ◽  
Luis Orlando Duitama Carreño ◽  
Rafael Keith Ono ◽  
Tássia Souza Bertipaglia ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Drift ◽  
Pedigree Analysis ◽  
Genetic Bottleneck ◽  
Brazilian Population ◽  
Pedigree Information ◽  
Effective Number ◽  
Inbreeding Coefficients ◽  
Brahman Cattle

Abstract: The objective of this work was to evaluate the genetic diversity of Brahman cattle in Brazil with pedigree analysis. Genealogical records of a subpopulation were used considering all pedigree information (Pt) and the pedigree information divided into two periods (P1, from 1994 to 2004; and P2, from 2005 to 2012) or according to the raising system (Ppt, animals on pasture; or Pst, on stable). Estimates were obtained for average inbreeding coefficients, generation intervals (GI), number of equivalent known generation (CGE), number of founders (Nf), effective number of founders (fe), effective number of ancestors (fa), and founder genome equivalents (fg). The average inbreeding coefficients were 11.97, 7.79, 11.95, 11.74, and 11.31% for Pt, P1, P2, Ppt, and Pst, respectively. Average GI was 4.4 years, whereas CGE was 3.18. The fe values were similar to those of fa, which were greater than those of fg. The fe/fa and fg/fe ratios were close to 1, which indicates no genetic bottleneck and small losses by genetic drift. The genetic diversity in the Brazilian population of Brahman breed is not significantly reduced.

scholarly journals Trunk perimeter correlates with genetic bottleneck intensity and the level of genetic diversity in populations of Taxus baccata L

2021 ◽  
Vol 78 (3) ◽  
Author(s):  
Sara Stefanowska ◽  
Katarzyna Meyza ◽  
Grzegorz Iszkuło ◽  
Igor J. Chybicki
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Demographic History ◽  
Genetic Bottleneck ◽  
Tree Age ◽  
Taxus Baccata ◽  
Census Size ◽  
Germplasm Collections ◽  
History Of ◽  
The Mean

Abstract Key message Taxus baccata remnants established recently tend to contribute less to the species’ overall genetic variation than historical populations because they are subjected to a greater impact of the founder effect and genetic isolation. As tree trunk perimeter is a rough indicator of genetic variation in a population, this measure should be considered in conservation programs. Context Genetic variation within Taxus baccata (L.) populations is not associated with the current census size but correlates well with the effective size, suggesting that genetic drift intensity reflects variation in demographic histories. Aims We hypothesize that recently established populations are subjected to greater bottleneck than old remnants. Using the mean trunk perimeter as a surrogate of tree age, we test whether the demographic history and genetic variation are associated with the mean tree age. Methods Using 18 microsatellite markers, we analyze the genetic diversity and demographic history of 11 yew populations in Poland to assess the relationship between the mean trunk perimeter and the inferred genetic parameters. Results Populations reveal significant differences in levels of genetic variation and in the intensity and time of genetic bottleneck. After excluding an apparent outlier, the genetic variation is significantly greater while the bottleneck intensity lower in populations with a greater perimeter. Conclusion Due to continuous species decline and increasing fragmentation, the non-uniform contribution of yew remnants to the overall genetic variation tends to decrease together with the mean tree age. Germplasm collections for the species should take into account tree perimeter as a rough indicator of the genetic variation of a population.

scholarly journals Genetic diversity and differentiation among Korean-Holstein, Hanwoo, and Uganda-Holstein breeds

2020 ◽  
Vol 49 (6) ◽  
pp. 1021-1027
Author(s):  
G Bigirwa ◽  
D Kim ◽  
O Acai ◽  
C Na ◽  
J Oh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dairy Cattle ◽  
Allelic Variation ◽  
Genetic Admixture ◽  
Factorial Correspondence Analysis ◽  
Expected Heterozygosity ◽  
The Mean ◽  
Polymerase Chain ◽  
Clear Differentiation ◽  
F Statistics

The aim of this research was to assess genetic diversity of Korean-Holstein, Korean Hanwoo, and Ugandan-Holstein dairy cattle. DNA was extracted from either blood or hair of Korean-Holstein (n=74), Korean-Hanwoo (n=75) and Ugandan-Holstein (N=77) using AccuPrep® PCR purification kit. The DNA samples were amplified by multiplex polymerase chain reaction, using GeneTrackTM Hanwoo genotyping kit and assayed using ABI genetic analyser 3130XL. Number of alleles, expected heterozygosity (He), observed heterozygosity (Ho), and the polymorphism information content (PIC) were estimated from 10 microsatellite loci in the three breeds. In addition, F-statistics for each of the 10 microsatellites in the three cattle breeds were estimated using fstat version 2.9.3.2 computer program. GENETIX (v.4.02) was used to perform factorial correspondence analysis (FCA) from the allele frequencies and multi-locus clustering was done using STRUCTURE analyses. A total of 124 alleles were detected. The number of alleles per locus varied from eight (TGLA126) to 22 (TGLA122), with an overall mean of 12.2. Expected heterozygosity ranged from 0.617 (SPS115) to 0.854 (TGLA53) and averaged 0.761. Observed heterozygosity ranged from 0.6 (SPS115) to 0.859 (TGLA53); and averaged 0.761. The mean PIC was 0.723; and means of the F-statistics FIT, FST and FIS were 0.077, 0.076 and 0.001 respectively. Although FCA revealed clear differentiation of Uganda-Holstein, Korean-Holstein, and Hanwoo, clustering assignments showed genetic admixture between Ugandan dairy cattle (Uganda-Holstein) and Hanwoo. In conclusion, the allelic variation present at the 10 loci was sufficient to categorize these cattle into distinct breed groups. Keywords: dairy cattle, genetic differentiation, F-statistics, heterozygosity, microsatellite

Monoamine Oxidase and Other Mitochondrial Enzymes in Density Subpopulations of Human Platelets

1988 ◽  
Vol 59 (01) ◽  
pp. 029-033 ◽  
Author(s):  
K G Chamberlain ◽  
D G Penington
Keyword(s):  
Monoamine Oxidase ◽  
Protein Concentration ◽  
Close Correlation ◽  
Human Platelets ◽  
Mitochondrial Enzymes ◽  
Density Fractions ◽  
Percoll Gradients ◽  
Normal Human ◽  
The Mean ◽  
Very High

SummaryNormal human platelets have been separated according to density on continuous Percoll gradients and the platelet distribution divided into five fractions containing approximately equal numbers of platelets. The mean volumes and protein contents of the platelets in each fraction were found to correlate positively with density while the protein concentration did not differ significantly between the fractions. Four mitochondrial enzymes (monoamine oxidase, glutamate dehydrogenase, cytochrome oxidase and NADP-dependent isocitrate dehydrogenase) were assayed and their activities per unit volume were found to increase in a very similar monotonie fashion with platelet density. When MAO and GDH were assayed on the same set of density fractions the correlation between the two activities was very high (r = 0.94–1.00, p <0.001) and a similar close correlation was found between MAO and ICDH. The results support the hypothesis that high density platelets either have a higher concentration of mitochondria or have larger mitochondria than low density platelets.

Der Sommer und Herbst 2003 aus phänologischer Sicht | Summer and autumn 2003 from a phenological point of view

2004 ◽  
Vol 155 (5) ◽  
pp. 142-145 ◽  
Author(s):  
Claudio Defila
Keyword(s):  
Time Series ◽  
New Record ◽  
Point Of View ◽  
Late Spring ◽  
Mean Deviation ◽  
Leaf Shedding ◽  
The Mean ◽  
Very High ◽  
Selection Of

The record-breaking heatwave of 2003 also had an impact on the vegetation in Switzerland. To examine its influences seven phenological late spring and summer phases were evaluated together with six phases in the autumn from a selection of stations. 30% of the 122 chosen phenological time series in late spring and summer phases set a new record (earliest arrival). The proportion of very early arrivals is very high and the mean deviation from the norm is between 10 and 20 days. The situation was less extreme in autumn, where 20% of the 103 time series chosen set a new record. The majority of the phenological arrivals were found in the class «normal» but the class«very early» is still well represented. The mean precocity lies between five and twenty days. As far as the leaf shedding of the beech is concerned, there was even a slight delay of around six days. The evaluation serves to show that the heatwave of 2003 strongly influenced the phenological events of summer and spring.

Growth table for Corsican pine in Flanders (Belgium)

1991 ◽  
Vol 56 ◽  
Author(s):  
B. Meuleman
Keyword(s):  
Insect Pests ◽  
Sandy Loam ◽  
Stand Density ◽  
Forest Tree ◽  
Permanent Plots ◽  
New Findings ◽  
Volume Increment ◽  
The Mean ◽  
Annual Volume ◽  
Very High

After  its introduction at the start of this century, the Corsican pine has become  an important forest tree in Flanders (Belgium). The total area covered by  Corsican pine is about 11.000 ha. Due to climatological factors it is  virtually absent from the Walloon part of Belgium. Despite the crisis in 1984  - 1986, practical experiences with its vitality and disease resistance are  generally positive. Compared to Scots pine which is native to Belgium, its  productivity and insensitivity for insect pests is large.     To quantify the productivity of Corsican pine, a growth table was  constructed using a method developed by PALM and DAGNELIE. It was based on  data from 321 temporarily and 80 permanent plots distributed over Flanders.  Five yield classes were distinguished according to dominant height at 50  years. For each yield class, a series of tables as a function of treatment  was constructed. Treatments were characterized by the mean annual  circumference increment.     These tables allow to predict the growth of Corsican pines. Such  predictions for the whole range of species are necessary for the development  of a good forestry policy and for timber industries. The tables also provide  information for any given Corsican pine stand that is helpful in practice:  expected productivity, stand density, determination of the felling quantum.      The data show that the productivity of Corsican pine is very high. The very  early culmination of the current annual volume increment and the rather  constant level of the mean annual volume increment after culmination are  interesting new findings. It is also shown that it is possible to work with  long rotation periods. This offers good opportunities for the production of  high quality wood and is also important for the social and ecological role of  the forest.     Productivity is lowest on very dry and sandy soils. A high productivity on  moderately dry sand and loamy sand soils and loamy soils make the Corsican  pine one of the most valuable tree species for the Kempen in Flanders.  Although productivity is very high on well drained sandy loam and loam soils,  plantation of Corsican pine on these locations is not advised.

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