Molecular tagging of botrytis grey mould disease in chickpea

2018 ◽  
Author(s):  
Ranjana . ◽  
Anju Arora ◽  
R. K. Panwar ◽  
S.K. Verma
Keyword(s):  
Resistance Gene ◽  
Hot Spot ◽  
Grey Mould ◽  
Disease Reaction ◽  
Natural Field ◽  
Molecular Tagging ◽  
Natural Exposure ◽  
Natural Field Condition ◽  
Polymorphic Bands ◽  
Moderately Resistant

Validation of two primers TA144 and TR29 for their linkage with botrytis grey mould resistance gene was studied in twenty genotypes of chickpea including resistant (GL10006) and susceptible (DCP92-3) checks. The genotypes were also scored for disease reaction under natural exposure at hot spot location. Molecular analysis with primers TA144 and TR29 showed polymorphic bands in GL10006 and DCP92-3. Therefore, it can be concluded that the primers are linked with resistance gene. The genotypes that showed amplicons with both the primers at same or nearby positions of resistant check were HK-2, HK-4, BG-1003, KAK-2 and GNG-1969 and indicated as resistant or moderately resistant ones. The above genotypes were also found resistant under natural field condition. Thus both the primers TA144 and TR29 showed good correlation with phenotypic evaluation in most of the genotypes studied. In future, more number of primers nearby the resistance gene(s) can be tried for their contribution towards overall disease reaction.

scholarly journals Molecular marker aided breeding for blast resistant rice in Nepal

2014 ◽  
Vol 15 ◽  
pp. 128-138
Author(s):  
Bal K Joshi ◽  
Hari P Bimb ◽  
Gopal Parajuli ◽  
Bedanand Chaudhary
Keyword(s):  
Resistance Gene ◽  
Ssr Markers ◽  
Bulked Segregant Analysis ◽  
Blast Resistance ◽  
Hot Spot ◽  
Resistance Breeding ◽  
Recurrent Parent ◽  
Simple Method ◽  
Multiple Alleles ◽  
Rice Landraces

Molecular markers tightly linked to target gene have been identified in different chromosomes to impose the genetic selection. This paper summarizes the progress and achievement made in breeding for blast resistance rice based on DNA markers. At least 40 genes conferring resistance to blast isolates with multiple alleles have been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Highly polymorphic and easily detectable SSR markers are being used in breeding for blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance gene by SSR markers. Quantitative trait loci (QTLs) have also been mapped and most of them are linked to qualitative genes. Simple sequence repeat (SSR) markers linked to the gene are being used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is necessary to initiate in Nepal focusing on resistance gene tagging in Nepalese rice landraces and utilization.

scholarly journals Disease reaction of potato germplasms and true potato seeds against Rhizoctonia solani Kuhn

1970 ◽  
Vol 40 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Md Maniruzzaman Khandaker ◽  
Abul Khair ◽  
Md Khurshed Alam Bhuiyan
Keyword(s):  
Rhizoctonia Solani ◽  
Field Condition ◽  
Potato Seed ◽  
True Potato Seed ◽  
Disease Reaction ◽  
Infested Field ◽  
Moderately Resistant

Screening of 25 potato germplasms against Rhizoctonia solani BTB115 in artificially infested field condition were carried out. No germplasm was found resistant to R. solani. Only six germplasms appeared to be moderately resistant. Cardinal, Diamant and Heera were found as highly susceptible varieties. True potato seed BARI TPS1 was tested against two virulent isolates of R. solani DK64 and BTB115. Only 21% and 29% resistant seedlings against the DK64 and BTB115 isolates, respectively were obtained compared to 85% seedlings in control.Keywords: Germplasm; Potato; TPS; Rhizoctonia solani   DOI: http://dx.doi.org/10.3329/bjb.v40i2.9777   Bangladesh J. Bot. 40(2): 193-196, 2011 (December)  

Identification and molecular tagging of a stripe rust resistance gene in wheat line P81

2010 ◽  
Vol 129 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Z. J. Pu ◽  
G. Y. Chen ◽  
Y. M. Wei ◽  
W. Y. Yang ◽  
Z. H. Yan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Wheat Line ◽  
Molecular Tagging ◽  
Stripe Rust Resistance Gene

Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris

Genome ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Fengqun Yu ◽  
Derek J. Lydiate ◽  
S. Roger Rimmer
Keyword(s):  
Brassica Napus ◽  
Linkage Group ◽  
Resistance Gene ◽  
Leptosphaeria Maculans ◽  
Resistance Locus ◽  
Dominant Allele ◽  
Breeding Lines ◽  
Disease Reaction ◽  
Blackleg Resistance ◽  
Map Location

The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus ‘Surpass 400’ are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified previously. ‘Surpass 400’ also has blackleg resistance introgressed from B. rapa subsp. sylvestris. Using 31 diverse isolates of L. maculans, the disease reaction of ‘Surpass 400’ was compared with those of the resistant breeding lines AD9 (which contains LepR1), AD49 (which contains LepR2), and MC1-8 (which contains both LepR1 and LepR2). The disease reaction on ‘Surpass 400’ was different from those observed on AD9 and MC1-8, indicating that ‘Surpass 400’ carries neither LepR1 nor both LepR1 and LepR2 in combination. Disease reactions of ‘Surpass 400’ to most of the isolates tested were indistinguishable from those of AD49, which suggested ‘Surpass 400’ might contain LepR2 or a similar resistance gene. Classical genetic analysis of F1 and BC1 plants showed that a dominant allele conferred resistance to isolates of L. maculans in ‘Surpass 400’. The resistance gene, which mapped to B. napus linkage group N10 in an interval of 2.9 cM flanked by microsatellite markers sR12281a and sN2428Rb and 11.7 cM below LepR2, was designated LepR3. A 9 cM region of the B. napus genome containing LepR3 was found to be syntenic with a segment of Arabidopsis chromosome 5.

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