Evaluation of combined effect of micronutrients and rhizobial inoculation on mungbean productivity

2019 ◽  
Author(s):  
Monika . ◽  
Priyanka . ◽  
Rajesh Yadav ◽  
LeelaWati .
Keyword(s):  
Enzyme Activity ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Ferrous Sulphate ◽  
Ammonium Molybdate ◽  
Indian Soils ◽  
Rhizobial Inoculation ◽  
Rhizobial Isolate ◽  
House Condition ◽  
Biological Nitrogen

Micronutrients play an important role in increasing yield of pulses and oilseed legumes through their effects on the plant itself and on the nitrogen fixing symbiotic process. Indian soils have become deficient not only in major plant nutrients like (NPK) but also scarce in some micronutrients like zinc, iron, molybdenum and boron. These situations suggest the need of application of micronutrients along with biofertilizers under intensive cultivation of legumes as it is directly involved in biological nitrogen fixation through nitrogenase enzyme activity. In the present research investigation, different micronutrients iron, molybdenum and their combination in the form of ferrous sulphate, ammonium molybdate and ferrous sulphate+ammonium molybdate @2.0, 2.0 and 2.5 kg/h are added into micronutrient deficient soil for plant growth promotion of mungbean under pot house condition. Among various treatments, ammonium molybdate application (@2.0kg/h along with rhizobial isolate HSR1 and PSB increases nodulation(28.6%), plant biomass (18.5%), seed yield (41%) as compared to sole application of biofertilizer.

scholarly journals Mitigation of Nickel Toxicity and Growth Promotion in Sesame through the Application of a Bacterial Endophyte and Zeolite in Nickel Contaminated Soil

2020 ◽  
Vol 17 (23) ◽  
pp. 8859 ◽  
Author(s):  
Muhammad Naveed ◽  
Syeda Sosan Bukhari ◽  
Adnan Mustafa ◽  
Allah Ditta ◽  
Saud Alamri ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soil ◽  
Root Zone ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Research Work ◽  
Control Treatment ◽  
Soil Conditions ◽  
Combined Application ◽  
Bioavailable Fraction

Nickel (Ni) bioavailable fraction in the soil is of utmost importance because of its involvement in plant growth and environmental feedbacks. High concentrations of Ni in the soil environment, especially in the root zone, may retard plant growth that ultimately results in reduced plant biomass and yield. However, endophytic microorganisms have great potential to reduce the toxicity of Ni, especially when applied together with zeolite. The present research work was conducted to evaluate the potential effects of an endophytic bacterium Caulobacter sp. MN13 in combination with zeolite on the physiology, growth, quality, and yield of sesame plant under normal and Ni stressed soil conditions through possible reduction of Ni uptake. Surface sterilized sesame seeds were sown in pots filled with artificially Ni contaminated soil amended with zeolite. Results revealed that plant agronomic attributes such as shoot root dry weight, total number of pods, and 1000-grains weight were increased by 41, 45, 54, and 65%, respectively, over control treatment, with combined application of bacteria and zeolite in Ni contaminated soil. In comparison to control, the gaseous exchange parameters (CO2 assimilation rate, transpiration rate, stomatal- sub-stomatal conductance, chlorophyll content, and vapor pressure) were significantly enhanced by co-application of bacteria and zeolite ranging from 20 to 49% under Ni stress. Moreover, the combined utilization of bacteria and zeolite considerably improved water relations of sesame plant, in terms of relative water content (RWC) and relative membrane permeability (RMP) along with improvement in biochemical components (protein, ash, crude fiber, fat), and micronutrients in normal as well as in Ni contaminated soil. Moreover, the same treatment modulated the Ni-stress in plants through improvement in antioxidant enzymes (AEs) activities along with improved Ni concentration in the soil and different plant tissues. Correlation and principal component analysis (PCA) further revealed that combined application of metal-tolerant bacterium Caulobacter sp. MN13 and zeolite is the most influential strategy in alleviating Ni-induced stress and subsequent improvement in growth, yield, and physio-biochemical attributes of sesame plant.

Pseudomonads contribute to regulation ofPratylenchus penetrans(Nematoda) populations on apple

2018 ◽  
Vol 64 (11) ◽  
pp. 775-785 ◽  
Author(s):  
Tristan T. Watson ◽  
Tom A. Forge ◽  
Louise M. Nelson
Keyword(s):  
Pseudomonas Fluorescens ◽  
Protease Activity ◽  
Fungal Pathogens ◽  
Plant Biomass ◽  
Growth Promotion ◽  
In Vitro Growth ◽  
Pratylenchus Penetrans ◽  
Pseudomonas Spp ◽  
Orchard Soil

Inoculation with antagonistic soil microorganisms has shown potential to suppress replant disease of apple in orchard soils. Pseudomonas spp. may have the potential to reduce Pratylenchus penetrans populations on apple. Pseudomonas spp. were isolated from the rhizosphere of sweet cherry and screened for antagonistic characteristics. Two highly antagonistic Pseudomonas isolates, P10-32 and P10-42, were evaluated for growth promotion of apple seedlings, suppression of P. penetrans populations, and root colonization in soil from three orchards. During the isolate screening, Pseudomonas fluorescens P10-32 reduced in vitro growth of fungal pathogens, had protease activity, had capacity to produce pyrrolnitrin, suppressed P. penetrans populations, and increased plant biomass. Pseudomonas fluorescens P10-42 reduced in vitro growth of fungal pathogens, had protease activity, suppressed P. penetrans populations, and increased plant biomass. In potted orchard soil, inoculating apple with P. fluorescens P10-32 suppressed P. penetrans populations in one of the three soils examined. Inoculation with P. fluorescens P10-42 improved plant growth in two of the soils and suppressed P. penetrans abundance in one soil. In one of the soils, P. fluorescens P10-42 was detected on the roots 56 days postinoculation. Overall, we conclude that Pseudomonas spp. play a role in suppressing P. penetrans on apple in orchard soil.

scholarly journals New Spectrophotometric Method for the Assessment of Catalase Enzyme Activity in Biological Tissues

2020 ◽  
Vol 16 (8) ◽  
pp. 1054-1062 ◽  
Author(s):  
Thulfeqar A. Hamza ◽  
Mahmoud H. Hadwan
Keyword(s):  
Hydrogen Peroxide ◽  
Enzyme Activity ◽  
Clinical Pathology ◽  
Biological Tissues ◽  
Ammonium Molybdate ◽  
Spectrophotometric Methods ◽  
Rate Limiting Step ◽  
Coefficients Of Variation ◽  
Rate Limiting ◽  
Health Applications

Background: Catalase is a vital antioxidant enzyme that dismutates H2O2 into water and molecular oxygen. Many protocols have been developed to measure catalase enzyme activity. Spectrophotometric methods are the most common assays that used to assess catalase enzyme activity. Methods: Because the rate-limiting step during catalase enzyme activity depends upon the dissociation of hydrogen peroxide, the developed assay measures the reaction between a hydroquinone/ anilinium sulfate/ammonium molybdate reagent and Unreacted Hydrogen Peroxide, which results in the production of a purple, disubstituted quinone compound with a maximum absorbance value at 550 nm. Results: To clarify the precision of the developed method, the coefficients of variation were determined to be 2.6% and 4.7% within run measurements and between run measurements, respectively. This method returned results that correlated well (r = 0.9982) with the results returned using the peroxovanadate method to assess catalase enzyme activity. Additionally, we examined the use of the newly developed hydroquinone assay to measure catalase enzyme activity in liver and bacterial homogenate samples. Conclusion: These results demonstrated that this assay can be used for scientific research and routine health applications because it is inexpensive, simple, accurate, and rapid. This method is suitable for use in clinical pathology laboratories because it is simple and produces precise and reproducible results.

Acute impacts of mixed heavy metals and diclofenac on sludge activity and enzyme activity involving with the biological nitrogen removal

2021 ◽  
Author(s):  
Yueyue Wang ◽  
Zichao Wang ◽  
Huan Yang ◽  
Youtao Song ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Enzyme Activity ◽  
Nitrogen Removal ◽  
Biological Nitrogen Removal ◽  
Synergistic Inhibition ◽  
Biological Nitrogen ◽  
Sludge Activity

Acute impacts of mixed heavy metals and diclofenac (DCF) on sludge activity and enzyme activity involving with the nitrogen removal were investigated. The synergistic inhibition on sludge activity and enzyme...

scholarly journals Sorghum Growth Promotion by Paraburkholderia tropica and Herbaspirillum frisingense: Putative Mechanisms Revealed by Genomics and Metagenomics

2020 ◽  
Vol 8 (5) ◽  
pp. 725 ◽  
Author(s):  
Eiko E. Kuramae ◽  
Stan Derksen ◽  
Thiago R. Schlemper ◽  
Maurício R. Dimitrov ◽  
Ohana Y. A. Costa ◽  
...  
Keyword(s):  
Plant Growth ◽  
Sorghum Bicolor ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Genomic Analysis ◽  
Shoot Biomass ◽  
Plant Growth Promoting ◽  
Plant Hormone Signaling ◽  
Underlying Mechanisms ◽  
High Degree

Bacteria from the genera Paraburkholderia and Herbaspirillum can promote the growth of Sorghum bicolor, but the underlying mechanisms are not yet known. In a pot experiment, sorghum plants grown on sterilized substrate were inoculated with Paraburkholderia tropica strain IAC/BECa 135 and Herbaspirillum frisingense strain IAC/BECa 152 under phosphate-deficient conditions. These strains significantly increased Sorghum bicolor cultivar SRN-39 root and shoot biomass. Shotgun metagenomic analysis of the rhizosphere revealed successful colonization by both strains; however, the incidence of colonization was higher in plants inoculated with P. tropica strain IAC/BECa 135 than in those inoculated with H. frisingense strain IAC/BECa 152. Conversely, plants inoculated with H. frisingense strain IAC/BECa 152 showed the highest increase in biomass. Genomic analysis of the two inoculants implied a high degree of rhizosphere fitness of P. tropica strain IAC/BECa 135 through environmental signal processing, biofilm formation, and nutrient acquisition. Both genomes contained genes related to plant growth-promoting bacterial (PGPB) traits, including genes related to indole-3-acetate (IAA) synthesis, nitrogen fixation, nodulation, siderophore production, and phosphate solubilization, although the P. tropica strain IAC/BECa 135 genome contained a slightly more extensive repertoire. This study provides evidence that complementary mechanisms of growth promotion in Sorghum might occur, i.e., that P. tropica strain IAC/BECa 135 acts in the rhizosphere and increases the availability of nutrients, while H. frisingense strain IAC/BECa 152 influences plant hormone signaling. While the functional and taxonomic profiles of the rhizobiomes were similar in all treatments, significant differences in plant biomass were observed, indicating that the rhizobiome and the endophytic microbial community may play equally important roles in the complicated plant-microbial interplay underlying increased host plant growth.

Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude Himalayan rhizosphere

Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Govindan Selvakumar ◽  
Piyush Joshi ◽  
Sehar Nazim ◽  
Pankaj Mishra ◽  
Jaideep Bisht ◽  
...  
Keyword(s):  
High Altitude ◽  
Phosphate Solubilization ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Early Report ◽  
Rrna Gene ◽  
Pseudomonas Fragi ◽  
Wheat Seedlings ◽  
Cold Temperatures ◽  
Psychrotolerant Bacterium

AbstractPhosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude garlic rhizosphere from the Indian Himalayas, are reported here. The identity of the isolate was arrived on the basis of its biochemical features and sequencing of the 16S rRNA gene. The isolate grew and solubilized phosphate at temperatures ranging from 4 to 30°C. Besides solubilizing P it produced indole acetic acid (IAA) and hydrogen cyanide (HCN). Seed bacterization with the isolate significantly increased the percent germination, rate of germination, plant biomass and nutrient uptake of wheat seedlings. While Pseudomonas fragi is normally associated with the spoilage of dairy products stored at cold temperatures, this is an early report on the plant growth promoting ability of the bacterium.

Biological nitrogen fixation and plant growth promotion of lodgepole pine by an endophytic diazotroph Paenibacillus polymyxa and its GFP-tagged derivative

Botany ◽  
2017 ◽  
Vol 95 (6) ◽  
pp. 611-619 ◽  
Author(s):  
Qian Tang ◽  
Akshit Puri ◽  
Kiran Preet Padda ◽  
Chris P. Chanway
Keyword(s):  
Plant Growth ◽  
Seedling Growth ◽  
Fluorescent Protein ◽  
Growth Promotion ◽  
Lodgepole Pine ◽  
Paenibacillus Polymyxa ◽  
Growth Enhancement ◽  
Plant Growth Promoting ◽  
Green Fluorescent ◽  
Biological Nitrogen

Paenibacillus polymyxa P2b-2R is an endophytic diazotroph originally isolated from lodgepole pine. It is reported to fix significant amounts of nitrogen (N) and promote plant growth. To evaluate the endophytic colonization sites, a green fluorescent protein (GFP) derivative of P2b-2R was generated (P2b–2Rgfp), but the effects of GFP modification on the functioning of P2b-2R have not been fully elucidated yet. In this study, we wanted to confirm and contrast the N-fixing and plant-growth-promoting abilities of P2b-2Rgfp with those of the wild-type P2b-2R in lodgepole pine. Pine seedlings were grown in an N-limited environment and harvested 2, 4, 8, and 12 months after inoculation to evaluate the endophytic and rhizospheric colonization by both strains, the amount of N-fixed, and seedling growth enhancement. Both P2b-2R and P2b-2Rgfp strains formed persistent rhizospheric and endophytic populations; fixed N; and enhanced seedling growth continuously after 4 months. P2b-2Rgfp-treated seedlings outperformed the P2b-2R-treated seedlings in terms of biomass only during the initial stages of plant development, but the differences decreased during the trial and were not significant towards the end. To the best of our knowledge, this is the first study that reports the effects of GFP-tagging of an endophyte when inoculated into a gymnosperm tree species.

scholarly journals Examining the Effects of the Nitrogen Environment on Growth and N2-Fixation of Endophytic Herbaspirillum seropedicae in Maize Seedlings by Applying 11C Radiotracing

2021 ◽  
Vol 9 (8) ◽  
pp. 1582
Author(s):  
Spenser Waller ◽  
Stacy L. Wilder ◽  
Michael J. Schueller ◽  
Alexandra B. Housh ◽  
Stephanie Scott ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Growth Conditions ◽  
Significant Inhibition ◽  
Cereal Crops ◽  
Herbaspirillum Seropedicae ◽  
Promote Plant Growth ◽  
Biological Nitrogen

Herbaspirillum seropedicae, as an endophyte and prolific root colonizer of numerous cereal crops, occupies an important ecological niche in agriculture because of its ability to promote plant growth and potentially improve crop yield. More importantly, there exists the untapped potential to harness its ability, as a diazotroph, to fix atmospheric N2 as an alternative nitrogen resource to synthetic fertilizers. While mechanisms for plant growth promotion remain controversial, especially in cereal crops, one irrefutable fact is these microorganisms rely heavily on plant-borne carbon as their main energy source in support of their own growth and biological functions. Biological nitrogen fixation (BNF), a microbial function that is reliant on nitrogenase enzyme activity, is extremely sensitive to the localized nitrogen environment of the microorganism. However, whether internal root colonization can serve to shield the microorganisms and de-sensitize nitrogenase activity to changes in the soil nitrogen status remains unanswered. We used RAM10, a GFP-reporting strain of H. seropedicae, and administered radioactive 11CO2 tracer to intact 3-week-old maize leaves and followed 11C-photosynthates to sites within intact roots where actively fluorescing microbial colonies assimilated the tracer. We examined the influence of administering either 1 mM or 10 mM nitrate during plant growth on microbial demands for plant-borne 11C. Nitrogenase activity was also examined under the same growth conditions using the acetylene reduction assay. We found that plant growth under low nitrate resulted in higher nitrogenase activity as well as higher microbial demands for plant-borne carbon than plant growth under high nitrate. However, carbon availability was significantly diminished under low nitrate growth due to reduced host CO2 fixation and reduced allocation of carbon resources to the roots. This response of the host caused significant inhibition of microbial growth. In summary, internal root colonization did little to shield these endophytic microorganisms from the nitrogen environment.

Sign in / Sign up

Export Citation Format

Share Document