scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) based Metabolomics of Promising Chickpea Genotypes against Callosobruchus chinensis (L.)

Author(s):  
M.S. Sai Reddy ◽  
Meena Agnihotri ◽  
S.D. Divija ◽  
Babita Bela ◽  
Somala Karthik

Background: The pulse beetle, Callosobruchus chinensis L. is one of the main biotic threats to chickpea in both field and storage in the world. The evaluation of chickpea genotypes for pulse beetle resistance has increased motivation for the identification and application of host plant resistance as a critical component of pest management. Methods: In the present study, different chickpea genotypes were evaluated under laboratory conditions to identify the antixenosis, antibiosis and GC-MS based untargeted metabolomics of promising chickpea genotypes to the C. chinensis. Result: Genotype NBeG1004 (36.61 eggs/100 seeds) was less prone to egg laying, while ICCV14872 (180.45 eggs/100 seeds) was the most preferred. However, maximal developmental periods, minimum per cent adult emergence and growth index was observed in ICCV92944 Genotype HC1 showed substantial resistance with maximum phenolic, flavonoid and protease inhibitors content. The results revealed that presence of 9-Octadecenoic acid (Z) in HC1 conferred resistance to C. chinensis. These results show assurance for incorporation into an IPM program against C. chinensis.

Author(s):  
Padmavati G Gore ◽  
K Tripathi ◽  
S K Chauhan ◽  
Mohar Singh ◽  
I S Bisht ◽  
...  

A total of 51 accessions of different Lens spp. viz., Lens culinaris (cultivated) and L. culinaris subsp. odemensis, L. culinaris subsp. orientalis, L. orientalis, L. nigricans, L. lamottei and L. ervoides (wild species) were screened for their reaction to Callosobruchus chinensis under no-choice artificial infestation conditions to find the sources of resistance. Significant differences among the accessions in terms of number of eggs laid, development period, adult emergence, number of emergence holes of C. chinensis loss in seed weight on different Lens spp. were observed. Of the seven species tested, L. culinaris was the most preferred one for egg laying while L. ervoides was the least preferred. Based on Growth Index (GI), accessions were categorised as highly resistant (15 accessions, GI = 0.00), resistant (14, GI=0.00 to 1.00), moderately resistant (16, GI=1.01 to 2.00) moderately susceptible (3, GI 2.01 to 3.00) susceptible (3, >3.01) to C. chinensis and the species have been arranged in the order of their resistance to C. chinensis. viz L. ervoides > L. lamottei > L. nigricans= L. orientalis > L. culinaris subsp. orientalis= L. culinaris subsp. odemensis > L. culnaris, i.e. L. ervoides was highly resistant and L. culnaris was the most susceptible. Correlation between GI and growth parameters of pulse beetle on different Lens spp. accessions indicated that GI had negative relationship with mean development period (-0.22) and significant positive relationship with adult emergence (+0.73), weight loss (+0.77) and eggs laid (+0.75).


2015 ◽  
Vol 38 (5) ◽  
Author(s):  
Kuldeep Tripathi ◽  
Sumit Kumar Chauhan ◽  
Padmawati G. Gore ◽  
T. V. Prasad ◽  
Kalyani Srinivasan ◽  
...  

A total of 52 accessions of cowpea including two checks (Pusa Komal and Local variety) were screened for resistance to pulse beetle, <italic>Callosobruchus chinensis</italic> under no-choice artificial infestation conditions. There were significant differences among the accessions in terms of number of eggs laid, development period, adult emergence, number of emergence holes, weight loss and growth index of <italic>C. chinensis</italic> on cowpea. Based on growth indices, Pusa Komal (0.04081) and IC328859 (0.04112) were resistant while IC106033 (0.06819) and Local variety (0.06816) were most susceptible to <italic>C. chinensis</italic>. Of the 52 accessions screeened, 11 accessions were resistant, 15 moderately resistant, 13 moderately susceptible, 8 susceptible and 5 were highly susceptible to <italic>C. chinensis.</italic> Correlation between growth index and growth parameters of pulse beetle on different cowpea accessions indicated that growth index had significant negative relationship with mean development period (r = -0.68) and significant positive relationship with adult emergence(r = +0.80). Adult emergence had a positive relationship with weight loss (r = +0.22).


1970 ◽  
Vol 4 (1) ◽  
pp. 89-93
Author(s):  
MZA Pramanik ◽  
MA Sardar

Experiments were carried out in the laboratory to assess the effectiveness of Nogos, Malathion, Sevin and Limper on lentil, gram, grasspea, greengram and blackgram seeds with eggs of pulse beetle, Callosobruchus chinensis L. Insecticides were sprayed on the seeds along with eggs and found that all the tested insecticides inhibited hatching of eggs resulting in significantly lowest rate of adult emergence. Nogos and Malathion recorded the lower number of emergent adults. The overall 26.35- 100% reduction of seed damage and 40-100% weight losses were achieved in insecticides treated seeds of five different types of pulses showing highest reduction in seed damage and weight losses due to Nogos and Malathion treatments. The treatment of the pulse seeds with the insecticides did not affect the seed germination. Key words: Insecticides, pulse beetle, egg treatment, pulses, seed damage.


1970 ◽  
Vol 8 (1) ◽  
pp. 35-38 ◽  
Author(s):  
MH Rahman ◽  
MA Ali ◽  
KS Ahmed

The experiment was conducted to find out the efficacy of dodder vine extract as seed protectant against pulse beetle, Callosobruchus chinensis on gram seed in the laboratory of the Department of Entomology, Bangladesh Agricultural University, Mymensingh during the period of February to May, 2009. The concentrations of dodder vine extract were 5, 2 and 1% respectively. Efficacies of these treatments were evaluated by considering oviposition, adult emergence, intensity of damage and seed weight loss done by pulse beetle. Dodder vine extract was found effective in checking oviposition, adult progeny development and severity of seed damage. Seeds treated with 5% concentration of dodder vine extract were less preferred for oviposition, adult emergence and seed weight loss by C. chinensis and this conc. might be useful in protection of pulse seed. Keywords: Dodder vine extract; Fecundity; Developmental performance; Seed weight loss; C. chinensis. DOI: 10.3329/jbau.v8i1.6395J. Bangladesh Agril. Univ. 8(1): 35-38, 2010


2020 ◽  
Vol 2 (1) ◽  
pp. p184
Author(s):  
Md. Israfil Jahan ◽  
Masum Ahmad ◽  
Md. Sohanur Rahman ◽  
Fakhar Uddin Talukder

The pesticidal efficacy of four locally available leaves of botanicals like neem (Azadirachta indica), datura (Datura stramonium),  marigold (Tagetes erecta) and garlic (Allium sativum L.) tablet powders was assessed on pulse bettle (Callosobruchus chinensis L) through adult mortality and emergence, oviposition performance, seed damage, weight loss and germination  of chickpea (Cicer arietinum). The above test biopesticides were tested at three different rates (0.5 g, 1.0 g and 1.50 g powder/kg of chickpea seeds). All the tested powder showed significant effect on the above parameters. The highest adult mortality was recorded when chickpea seeds were treated with datura leaf powder at 1.50 g/kg, whereas marigold showed the least effect on adult mortality. The powders used in the experiment effectively controlled oviposition and adult emergence of pulse beetle. The lowest number of adult emergence was obtained from chickpea seeds treated with datura leaf powder at 1.50 g/kg. The highest weight loss was observed in control seeds followed by marigold leaf powder 0.5 g/kg gram seeds. Datura leaf powder treated seeds showed the lowest seed weight loss followed by neem, garlic and marigold. Plant powders had profound effect on seed germination. The highest germination was observed in treated seed with datura leaf powder at 1.50 g/kg and the lowest germination in control seed followed by marigold leaf powder at 1.5 g/kg treated seeds. The pesticidal effect on oviposition, adult mortality, adult emergence, and seed weight loss was directly proportional to leaf powder rates.


2018 ◽  
Vol 24 (2) ◽  
Author(s):  
PRAHALAD MANDLOI ◽  
S.P.S. TOMAR ◽  
PRADYUMN SINGH ◽  
N.K.S. BHADAURIA ◽  
N.S. BHADAURIA

Maximum fecundity was found on genotype RVSSG-38. The adult emergence on different genotypes ranged from 7.0 to 18.0. Genotype RVSSG-44 recorded maximum percent loss in seed weight. Genotype RVSSG-43 had minimum percentage of seed infestation. Susceptibility index of pulse beetle on different genotypes of chickpea showed that there were no significant differences among different genotypes. Infestation percentage positively and significantly associated with protein content in fresh seed and infested seed. Seed size and seed shape of the genotypes did not influence the fecundity, adult emergence, total development period and susceptibility index of pulse beetle.


2013 ◽  
Vol 12 ◽  
pp. 81-90
Author(s):  
Homan Regmi ◽  
Yubak Dhoj

An experiment on eco-friendly approach of managing pulse beetle (Callosobruchus chinensis L.) in chickpea (Cicer arietinum L) was carried out at the Institute of Agriculture and Animal Science (IAAS), Rampur, Chitwan, Nepal from April to July, 2007. The experiment consisted of fourteen treatments laid out in two factors Completely Randomized Design (CRD) with three replications. Among them, storage structure (aluminium sheet bin and jute bag with plastic lining) was considered as one factor and pesticidal materials (Xanthoxylum armatum DC fruit dust@ 30gm kg-1; Acorus calamus L. rhizome dust@ 30 gm kg-1; Cinnamom camphora balls@ 1.5 gm kg-1; Sesamum indicum L. oil@ 5 ml kg-1; Azadirachta indica A. Juss. leaf dust@30gm kg-1; malathion dust@ 1gm kg-1 and control) was as another factor. Among six pesticidal materials tested, mortality of adult bruchids was obtained significantly higher in chickpea treated by C. camphora balls (25.17) followed by A. calamus rizome dust (24.83) and Sesamum oil (24.50). Grain damage and weight loss was nil in chickpea treated by these three materials. Among botanicals used, X. armatum fruit dust and A. indica leaf dust were found ineffective to cause adult mortality (20.33 and 19.67) and adult emergence (1549 and 2292) in chickpea. Malathion, initially was found somewhat effective in adult mortality (21.67) and adult emergence (30.33). However, it was less effective from F2 generation resulting maximum adult emergence (2127). While comparing two storage structures, jute bag with plastic lining was better in terms of maintaining lower moisture (14.64%), higher germination (65.14%) and lesser grain damage (39.95%). Thus, the jute bag with plastic lining was found as a better storage structure and botanical materials such as A. calamus rhizome dust, Sesamum oil and C. camphora balls have been found as effective safe alternatives for the management of C. chinensis L. in the storage of chickpea. The Journal of Agriculture and Environment Vol:12, Jun.2011, Page 81-90 DOI: http://dx.doi.org/10.3126/aej.v12i0.7567


2018 ◽  
Vol 24 (2) ◽  
Author(s):  
PRAHALAD MANDLOI ◽  
PRADYUMN SINGH ◽  
S.P.S. TOMAR ◽  
N.K.S. BHADAURIA ◽  
V.K. SHRIVASTAVA

Twelve genotypes with variation in seed size, seed weight, seed coat colour and seed shape were included for the study. Genotype RVSSG-44 recorded maximum percent loss in seed weight. Genotype RVSSG-43 had minimum percentage of seed infestation, which was significantly less than rest of the genotypes, except JG-130 seed infestation. Susceptibility index of pulse beetle on different genotypes of chickpea showed that there were no significant differences among different genotypes. Infestation percentage positively and significantly associated with protein content in fresh seed and infested seed. However, other parameters were not associated with infestation by beetle. It ranged from 11.7 to 13.0 adult on angular and pea shape seeded genotypes, respectively. Seed size and seed shape of the genotypes did not influence the fecundity, adult emergence, total development period and susceptibility index of pulse beetle.


2016 ◽  
Vol 41 (1) ◽  
pp. 75-84 ◽  
Author(s):  
MA Ahad ◽  
MK Nahar ◽  
MR Amin ◽  
SJ Suh ◽  
YJ Kwon

The n-hexane extracts of the weeds ‘bhatpata’ Clerodendrum viscosum, ‘kashiature’ Cassia tora, ‘dhakishak’ Dryoptris filix-max, ‘bonmorich’ Croton bonpalandianum and ‘ghagra’ Xanthium strumarium were used to evaluate their effectiveness for suppressing pulse beetle, Callosobruchus chinensis reared on mung bean Vigna radiata grains. The investigations were done with 1, 2 and 4% n-hexane extracts of the weeds and an untreated control. The weed extracts exhibited considerable effectiveness which varied with weed species, concentrations and exposure durations. The higher concentrations showed the higher rate of insect mortality, fecundity, adult emergence inhibition, and grain protection. The LC50 values of the extracts ranged from 5.3 to 7.8, 4.7 to 6.5 and 4.1 to 6.0 g/100 ml at 24, 48 and 72 hours after treatment, respectively. The fecundity inhibition varied from 31.7 to 78.7%, adult emergence inhibition from 33.8 to 81.1%, and grain damage inhibition from 10.3 to 60.1% when ‘bhatpata’ with concentration of 1 g/100 ml and ‘ghagra’ with concentration of 4g/100 ml were applied, respectively. Among the tested weeds, ghagra (4g/100 ml) showed better efficacy against C. chinensis compared to other tested extracts and may be suggested to control pulse beetle and protection of mung bean grains.Bangladesh J. Agril. Res. 41(1): 75-84, March 2016


2020 ◽  
Vol 17 ◽  
Author(s):  
Jyoti Gaba ◽  
Sunita Sharma ◽  
Pardeep Kaur ◽  
Sukesha Joshi

Abstract:: In the present study, different derivatives of thymol (1) viz. hydrazide (2), oxadiazole thiol (3), triazole thione (4), hydrazones (5-7) and β-lactams (8-10) were synthesized. All synthesized compounds were identified and characterized using elemental analysis, UV-Visible, 1H NMR, 13C NMR, IR spectroscopic techniques. Synthesized thymol derivatives were evaluated for antifungal potential against phytopathogenic fungi Fusarium moniliforme, Rhizoctonia solani, Dreschlera madis of maize in comparison to recommended standards in terms of percent inhibition and ED50 values. Thymol was more effective as compared to its derivatives against all three tested fungi. Hydrazones (5-7) and β-lactams (8-10) having m-NO2 substituted phenyl ring (6, 9) were less effective as compared to o-NO2 and p-NO2 analogues against F. moniliforme and R. solani, however reverse trend was observed against D. maydis. Thymol and its derivatives were also tested for insecticidal activity against stored grain (chickpea) insect Callosobruchus chinensis and various parameters viz. egg laying, adult emergence and grain damage were recorded and compared. Compounds having oxadiazole thiol (3) and triazole thione (4) moiety showed promising effects against insect C. chinensis.


Sign in / Sign up

Export Citation Format

Share Document