Galactomannan content and key enzymes of its metabolism in seedsof cluster bean [Cyamopsis tetragonoloba (L.) Taub.]

2016 ◽  
Author(s):  
Neha Wadhwa ◽  
Udai Narayan Joshi
Keyword(s):  
Guar Gum ◽  
Specific Activity ◽  
Genetic Manipulation ◽  
Sharp Decrease ◽  
Cyamopsis Tetragonoloba ◽  
Key Enzymes ◽  
Cluster Bean ◽  
Activity Of Enzymes ◽  
The Mean ◽  
Mature Seeds

The present investigation was carried out to estimate galactomannan content in mature seeds of 17 guar genotypes and activity of enzymes involved in galactomannan metabolism. Galactomannan content was found in the range of 16.82 (in IC 310630) to 36.68 per cent (in HG 3-2). The developing pods were sampled at 25, 32, 39 and 46 days after flowering (DAF) for a-galactosyltransferase, ß-D-mannosidase & ß-1, 4-mannanase assay. The mean a-galactosyltransferase specific activity increased from 25 to 39 DAF (1557 to 3093 units) followed by decrease at 46 DAF (1484 units). The mean specific activity increased from 392 to 3166 units with the increase in galactomannan content from 16.82 to 36.68 per cent. Thus, this enzyme showed highly positive correlation with the galactomannan content. The mean specific activity of ß-D-mannosidase increased gradually from 25 to 39 DAF (67 to 138 units) followed by sharp decrease at 46 DAF (32 units). The mean specific activity of ß-1, 4-mannanase was found maximum at 25 DAF (102 units) and afterwards, it decreased continuously with advancement of days after flowering up to 46 days (9 units). On the whole, it can be said that the ß-D-mannosidase requires prior activity of ß-1, 4-mannanase for galactomannan catabolism while a-galactosyltransferase activity is positively correlated with galactomannan content and play a major role in guar gum synthesis and can be further used for gum improvement via genetic manipulation.

Cluster bean [cyamopsis tetragonoloba (L.) Taub.], an importantindustrial arid legume: A review

2016 ◽  
Author(s):  
R. K. Bhatt ◽  
A. K. Jukanti ◽  
M. M. Roy
Keyword(s):  
Tamil Nadu ◽  
Guar Gum ◽  
Cyamopsis Tetragonoloba ◽  
Marginal Lands ◽  
Well Drilling ◽  
Cluster Bean ◽  
Beneficial Effects ◽  
Global Cluster ◽  
Gum Content ◽  
Commercial Applications

Cluster bean is an important legume cultivated mostly on marginal and sub marginal lands of arid and semi-arid regions. Overall, India produces around 80% of global cluster bean production. It is cultivated on more than 4 m ha in India, Rajasthan alone accounts for around 80% of the area and production. Owing to its demand in the international market, it has been introduced in the non-traditional growing areas like Andhra Pradesh, Tamil Nadu, Karnataka, Maharashtra and Chhattisgarh. Further, its cultivation is also being taken up under irrigated conditions during summer. Cluster bean seed (endosperm) is a source of a natural hydrocolloid (galactomannan/‘guar gum’). The gum has unique abilities with multiple commercial applications like textile, printing, paper, cosmetics, mining, pharmaceutical, petroleum, natural gas, well drilling and oil industries. Green pods are nutritionally rich and are routinely consumed as vegetable especially in northern and western parts of India. Additionally, cluster bean has been reported to have beneficial effects when cultivated as an intercrop. Cluster bean meal (high protein content) obtained from seed coat and germ cell is an excellent feed for monogastric animals. Cluster bean cultivars with high gum content (>32%) and viscosity (4000-5000 cps) are more preferred for export. The value of cluster bean exports from India has increased from Rs 242 crores (1994) to Rs 2100 crores (2012-13). This review presents the various aspects of cluster bean cultivation and its emergence as an important industrial crop.

scholarly journals The Evaluation of Cluster Bean (Cyamopsis Tetragonoloba (L.) Taub) Genotypes for Fruit and Gum Yield

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
R. Balakumbahan ◽  
G. Prabukumar ◽  
V. Sivakumar
Keyword(s):  
Seed Yield ◽  
Guar Gum ◽  
Growth Yield ◽  
Cyamopsis Tetragonoloba ◽  
Vegetable Crops ◽  
Fruit Length ◽  
Cluster Bean ◽  
Check Variety ◽  
Gum Content ◽  
Number Of Seeds

An experiment on evaluation of cluster bean genotypes was conducted at the department of vegetable crops, Horticultural College and Research Institute, Periyakulam. Fifty genotypes of gum type of cluster bean from NBPGR, Regional Station Jodhpur, two varieties viz., Thar Bhadavi and the check variety Pusa Navbahar were collected for the study. The collected fifty two (Ct - 1 to Ct - 52) genotypes were evaluated for their growth, yield and gum content for three seasons from November 2013 to February 2015. The data collected from the 52 genotypes. The three seasons data were pooled and statistically analysed. From the above study it is concluded that the variety Pusa Navbahar (Ct – 52 check variety) recorded highest total fruit yield per plant, fruit length, number of seeds per fruit and seed yield per plant than other genotypes taken for evaluation. The same genotype Ct - 52 also recorded the highest guar gum content of 13.44 per cent followed by 12.94 per cent in Ct – 39 and the lowest gum yield of 5.79 per cent was observed in Ct - 47.

An Economic Analysis of Cluster Bean Production in Bikaner District of Rajasthan

2020 ◽  
pp. 1-6
Keyword(s):  
Guar Gum ◽  
Animal Feed ◽  
The State ◽  
Total Output ◽  
Cyamopsis Tetragonoloba ◽  
Small Farm ◽  
Cluster Bean ◽  
Legume Crop ◽  
Large Farm ◽  
Bean Crop

Cluster Bean (Cyamopsis tetragonoloba L. Taub) commonly known as is a drought and high temperature tolerant Cyamopsis tetragonoloba Guar, deep-rooted summer annual legume crop cultivated mainly in the Kharif season in an arid region and used as animal feed, fodder, green manure and extraction of gum for industrial uses. India is the largest producer of cluster bean and contributes 80 percent of total cluster bean production in the world. Rajasthan is the single largest producer and alone contributed to more than 70 percent of India’s total output. The increased demand for guar gum resulted in a strong escalation of the prices of cluster bean. Looking at the agricultural and industrial importance of this marginalized crop in Rajasthan, particularly realizing the potential in Bikaner district of the state, the present study attempted to analyze the present scenario of the production of cluster bean crops in Bikaner district of the state. A return per rupee invested in cluster bean crop was higher in the small farm (1:1.38) and marginally lowers on the medium farm (1:1.35). Break-even guar production ranged between 4.26 to 5.19 q per ha in small farm, 4.16 to 4.99 q per ha in medium and 3.98 to 4.94 q per ha on a large farm.

scholarly journals Chromosome scale reference genome of Cluster bean (Cyamopsis tetragonoloba (L.) Taub.)

2020 ◽  
Author(s):  
Kishor Gaikwad ◽  
G. Ramakrishna ◽  
Harsha Srivastava ◽  
Swati Saxena ◽  
Tanvi Kaila ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Reference Genome ◽  
Guar Gum ◽  
Western India ◽  
Cyamopsis Tetragonoloba ◽  
Protein Coding ◽  
Cluster Bean ◽  
Gas Drilling ◽  
Oxford Nanopore ◽  
A Genome

AbstractClusterbean (Cyamopsis tetragonoloba (L.) Taub.), also known as Guar is a widely cultivated dryland legume of Western India and parts of Africa. Apart from being a vegetable crop, it is also an abundant source of a natural hetero-polysaccharide called guar gum or galactomannan which is widely used in cosmetics, pharmaceuticals, food processing, shale gas drilling etc. Here, for the first time we are reporting a chromosome-scale reference genome assembly of clusterbean, from a high galactomannan containing popular guar cultivar, RGC-936, by combining sequenced reads from Illumina, 10x Chromium and Oxford Nanopore technologies. The initial assembly of 1580 scaffolds with an N50 value of 7.12 Mbp was generated. Then, the final genome assembly was obtained by anchoring these scaffolds to a high density SNP map. Finally, a genome assembly of 550.31 Mbp was obtained in 7 pseudomolecules corresponding to 7 chromosomes with a very high N50 of 78.27 Mbp. We finally predicted 34,680 protein-coding genes in the guar genome. The high-quality chromosome-scale cluster bean genome assembly will facilitate understanding of the molecular basis of galactomannan biosynthesis and aid in genomics-assisted breeding of superior cultivars.

scholarly journals SYNTHESIS AND MIGRATION OF PROTEINS IN THE CELLS OF THE EXOCRINE PANCREAS AS REVEALED BY SPECIFIC ACTIVITY DETERMINATION FROM RADIOAUTOGRAPHS

1963 ◽  
Vol 16 (1) ◽  
pp. 1-23 ◽  
Author(s):  
H. Warshawsky ◽  
C. P. Leblond ◽  
B. Droz
Keyword(s):  
Specific Activity ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Zymogen Granule ◽  
Zymogen Granules ◽  
The Mean ◽  
And Migration ◽  
Rats And Mice ◽  
Silver Grains ◽  
Golgi Zone

Radioautographs of pancreatic acinar cells were prepared in rats and mice sacrificed at various times after injection of leucine-, glycine-, or methionine-H3. Measurements of radioactivity concentration (number of silver grains per unit area) and relative protein concentration (by microspectrophotometry of Millon-treated sections) yielded the mean specific activity of proteins in various regions of the acinar cells. The 2 to 5 minute radioautographs as well as the specific activity time curves demonstrate protein synthesis in ergastoplasm. From there, most newly synthesized proteins migrate to and accumulate in the Golgi zone. Then they spread to the whole zymogen region and, finally, enter the excretory ducts. An attempt at estimating turnover times indicated that two classes of proteins are synthesized in the ergastoplasm: "sedentary" with a slow turnover (62.5 hours) and "exportable" with rapid turnover (4.7 minutes). It is estimated that the exportable proteins spend approximately 11.7 minutes in the Golgi zone where they are built up into zymogen granules, and thereafter 36.0 minutes as fully formed zymogen granules, before they are released outside the acinar cell as pancreatic secretion. The mean life span of a zymogen granule in the cell is estimated to be 47.7 minutes.

Effect of Organic Nutrient Management on Growth and Yield of Cluster Bean (Cyamopsis tetragonoloba L.)

2021 ◽  
Author(s):  
J.H. Brahmbhatt ◽  
S.K. Acharya ◽  
G.S. Patel ◽  
Chirag Thakar ◽  
N.R. Solanki
Keyword(s):  
Nutrient Management ◽  
Block Design ◽  
Organic Production ◽  
Growth And Yield ◽  
Cyamopsis Tetragonoloba ◽  
Cluster Number ◽  
Organic System ◽  
Cluster Bean ◽  
Pod Yield ◽  
Organic Nutrient

Background: Organic production of cluster bean is to contribute to the enhancement of production sustainability along with ecology. Sustainability in organic farming must therefore be seen in a holistic sense, which includes ecological, economic and social aspects. A synergistic effectof bio fertilizer with crop increases the crop productivity and sustainability also. Bio fertilizers are low cost, effective and renewable sources of plant nutrients to supplement chemical fertilizers. Therfore to overcome the ill effect of chemical based farming, organic system is becoming the emerging need of the society.Methods: A field experiment ’’was carried out in Pusa Navbahar variety of clusterbean during summer season (February to May) of 2019 at College farm, College of Horticulture, Sardarkrushinagar Dantiwada Agricultural University, Jagudan, Dist. Mehsana, Gujarat, India. Sixteen treatments having various combinations of organic sources of nutrients (FYM, vermicompost, and neem cake), bio fertilizers (Rhizobium + PSB + KSM) along with RDF (20/40/0 kg/ha) as a control. They were replicated thrice in a randomized block design having a plot size of 3.0 m × 2.0 m with a spacing of 60 cm × 20 cm. Standard practices were followed during the entire course of the investigation.Result: It showed highly significant differences among different treatments for majority of the growth and yield attributing traits, but treatment T6 (75% N through FYM + Rhizobium + PSB + KSM) was rated as the best treatment for characters like days taken for germination, number of root nodules per plant, plant height at 60 DAS (cm), number of pod per cluster, number of cluster per plant, green pod yield per plant (g), green pod yield per plot (kg) and green pod yield per hectare (kg) as compared to other treatment. It can be concluded that the organic nutrient management in cluster bean with the application of 75% N through FYM + Rhizobium + PSB + KSM is beneficial for obtaining a higher yield.

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