The last missing piece of the Triangle of U: the evolution of the tetraploid Brassica carinata genome

Ethiopian mustard (Brassica carinata) is an ancient crop with significant potential for expanded cultivation as a biodiesel feedstock. The remarkable stress resilience of B. carinata and desirable seed fatty acid profile addresses the ongoing food vs. fuel debate as the crop is productive on marginal lands otherwise not suitable for even closely related species. B. carinata is one of six key Brassica spp. that share three major genomes: three diploid species (AA, BB, CC) that spontaneously hybridized in a pairwise manner, forming three allotetraploid species (AABB, AACC, and BBCC). Each of these genomes has been researched extensively, except for that of B. carinata. In the present study, we report a high-quality, 1.31 Gbp genome with 156.9-fold sequencing coverage for B. carinata var. Gomenzer, completing and confirming the classic Triangle of U, a theory of the evolutionary relationships among these six species that arose almost a century ago. Our assembly provides insights into the genomic features that give rise to B. carinata's superior agronomic traits for developing more climate-resilient Brassica crops with excellent oil production. Notably, we identified an expansion of transcription factor networks and agronomically-important gene families. Completing the Triangle of U comparative genomics platform allowed us to examine the dynamics of polyploid evolution and the role of subgenome dominance in domestication and agronomical improvement.

A Systematic Review on Opuntia (Cactaceae; Opuntioideae) Flower-Visiting Insects in the World with Emphasis on Mexico: Implications for Biodiversity Conservation

Opuntia spp. are cacti with high ecological, economic and conservation interest in semiarid environments, particularly in Mexico. We conducted a systematic search of the existing peer-reviewed literature about the state of knowledge of pollination ecology on these plants. We documented the most studied Opuntia species worldwide with an emphasis on Mexico. We found that only 15% of Opuntia species described have been investigated so far, and studies were mainly focused on comprehension of the biology of a single species. Despite the economic and cultural importance of Opuntia, there is a significant lack of knowledge about the flower-visiting insects and their taxonomic identity. We provide a checklist of the insect species associated with Opuntia spp. Through a circular network, we visualize the complex Opuntia flower-visiting insect relationship, and we detected a set of key species constituting the generalist core of the networks constructed. Since pollination is crucial for crop production, a better understanding of ecological interactions would inform management measures to strengthen biodiversity and agriculture sustainability as well as productivity in arid and marginal lands. Further research on pollination ecology is needed to improve the conservation status of the insects associated with Opuntia species.

High-Grade Chemicals and Biofuels Produced from Marginal Lands Using an Integrated Approach of Alcoholic Fermentation and Pyrolysis of Sweet Sorghum Biomass Residues

New global directions align agricultural land resources towards food production; therefore, marginal lands could provide opportunities for second-generation energy crops, assuming that in the difficult conditions of plant development, productivity can be maintained at relatively high levels. Sustainable bioenergy production on marginal lands represents an ambitious objective, offering high-quality biofuels without competing with the agri-food industry, since it allows successful feedstock production to be performed on unmanaged areas. However, marginal land feedstock production generally shows several agronomic, techno-economic, and methodological challenges, leading to decreases in the obtained quantities of biomass and profitability. Sweet Sorghum is a technical plant that has the needed qualities to produce large amounts of biofuels on marginal lands. It is a high biomass- and sugar-yielding crop, characterized by a high photosynthetic efficiency and low fertilizer requirement, is resistant to drought, and adapts well to different climate areas. Marginal lands and contaminated soils provide a favorable development environment for plants such as sweet sorghum; however, in-depth research studies on biomass productivity must be carried out, as well as advanced quality evaluation of the products, in order to develop combined technologies that use resources efficiently. The present study starts with a comparative evaluation of two sweet sorghum crops established on both marginal and regular lands, assessing plant development characteristics and juice production, and an evaluation of bioethanol generation potential. The vegetal wastes resulting from the processing were treated by pyrolysis, with the aim of maximizing the productivity of high-quality liquid biofuels and chemicals. The charcoal obtained in the thermal processes was considered as an amendment of the soil so that marginal land quality could be improved over time.

Simultaneous Carbon Storage in Arable land and Anthropogenic Products (CSAAP): demonstrating a new concept towards well below 2°C

The removal of additional carbon dioxide from the atmosphere is indispensable for controlling global warming. This study proposed the concept of ‘biopump’, as plants capable of significantly transferring carbon into the soil. The Carbon Storage in Arable land and Anthropogenic Products (CSAAP) relates to the cultivation of ‘biopumps’ on marginal arable lands poor in soil organic carbon (SOC) and their conversion into long-lived anthropogenic products. Based on a list of twenty-seven biopumps assembled from a literature review, this study proposed a method for the regional prioritization of biopumps, considering among others their ability to increase SOC and adaptation. A list with eight woody and eight herbaceous biopumps was recommended for France. To illustrate the potential of the CSAAP strategy for products encompassing a variety of lifetimes, carbon flows, from biopump cultivation to biomaterial manufacturing and end-of-life, were tracked in time to calculate their influence on global mean temperature change. An illustration was performed on the basis of a French case study, where Miscanthus is grown on spatially identified marginal lands quantified as 11,187- 24,007 km2. Planting biopumps on these lands could increase by 0.23 to 0.49 Mt carbon stocked as SOC annually, which represents 0.19%- 0.41% of the annual French carbon budget during 2015-2018. If the carbon contained in the biomass is indefinitely kept in anthropogenic products, it could represent 13.07% of the same carbon budget. We concluded that biopumps could induce negative emission by 2100, with efficiency strongly depending upon carbon’ residence time in the anthroposphere.

RECYCLING BIOSOLIDS TO IMPROVE MARGINAL LANDS FOR BIOENERGY FEEDSTOCK PRODUCTION IN UKRAINE

Energy independence is one of the national priorities facing Ukraine today. Plant-based feedstocks have the potential to diversify Ukraine’s energy independence by decreasing dependence on petroleum-based energy, reducing greenhouse gas emissions, expanding renewable fuel industries and creating job opportunities. However, biofeedstock needs to be competitive on availability, performance, and price to produce, market, and produce fuels. We hypothesize that domestically produced feedstocks from sweet sorghum, using proactive recycling of nutrient-rich biosolids on vast areas of degraded and marginal lands, could be a win-win energy independence strategy in Ukraine. Our goal is to create for generating a steady-state source of biofeedstock and disseminate science-based knowledge and training to the clientele. Specific objectives are to: (1) establish research studies to evaluate growth and feedstock productivity, nutrient removal, and feedstock characteristics of sweet sorghum fertilized with biosolids on degraded and marginal lands in Rivne, Kherson, Dnipro, and Kyiv regions of Ukraine; and (2) determine the impact of biosolids and sweet sorghum on soil quality. Data collected on growth, feedstock production, feedstock characteristics, fuel potential, and high-value co-products (biochar) of sweet sorghum and soil quality will be evaluated by multivariate statistics. Input, output, and outreach data will be subject to techno-economic analyses to evaluate the economically viability, environmentally compatibility, and social acceptability of the project. Traditional and electronic outlet activities will be utilized to disseminate outcomes and outputs and to evaluate project impacts.

Complex polyploid and hybrid species in an apomictic and sexual tropical forage grass group: genomic composition and evolution in Urochloa (Brachiaria) species

Background and Aims Diploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa are important for food security and the environment​, often being planted in marginal lands worldwide. We aimed to characterize the nature of their genomes, the repetitive DNA, and the genome composition of polyploids, leading to a model of the evolutionary pathways within the group including many apomictic species. Methods Some 362 forage grass accessions from international germplasm collections were studied, and ploidy determined using an optimized flow cytometry method. Whole-genome survey sequencing and molecular cytogenetic analysis were used to identify chromosomes and genomes in Urochloa accessions belonging to the 'brizantha' and 'humidicola' agamic complexes and U. maxima. Key Results Genome structures are complex and variable, with multiple ploidies and genome compositions within the species, and no clear geographical patterns. Sequence analysis of nine diploid and polyploid accessions enabled identification of abundant genome-specific repetitive DNA motifs​. In situ hybridization with a combination of repetitive DNA and genomic DNA probes, identified evolutionary divergence and allowed us to discriminate the different genomes present in polyploids. Conclusions We suggest a new coherent nomenclature for the genomes present​. We develop a model of evolution at the whole-genome level in diploid and polyploid accessions showing processes of grass evolution. We support the retention of narrow species concepts for U. brizantha, U. decumbens, and U. ruziziensis, and do not consider diploids and polyploids of single species as cytotypes. The results and model will be valuable in making rational choices of parents for new hybrids, assist in use of the germplasm for breeding and selection of Urochloa with improved sustainability and agronomic potential, and will assist in measuring and conserving biodiversity in grasslands.

Multi-Criteria Decision-Making Approach for Nutraceuticals Greener Applications: The Cynara cardunculus Case Study

Nutraceuticals are an ever-expanding market worldwide, facing the unstoppable transition towards a green economy. Developing economically feasible and sustainable alternatives to current raw materials for the extraction of nutraceuticals is, therefore, essential to reach these goals and, at the same time, achieve social and economic competitiveness. This paper intends to propose an economical and environmentally sustainable feedstock for chlorogenic acid (CGA) and inulin, whose current extraction from green coffee and chicory, respectively, is unsustainable. Our approach is based on the multi-criteria decision-making approach (MCDA), supported by the analytical hierarchy process (AHP), ranking the performance of competitor biomasses according to economic, social, and technological criteria. The results of this study highlight cardoon (Cynara cardunculus) as a promising raw material for the extraction of CGA and inulin in virtue of the high concentration, low-input growth regime, and the possibility of being grown on marginal lands. Nevertheless, cardoon biomass availability is currently scarce, extraction methods are underdeveloped, and consequently, the obtained product’s price is higher than the benchmark competitors. Policies and investments favoring sustainable cultivations could stimulate cardoon employment, linking economic advantages and land requalification while limiting phenomena such as desertification and food competition in the Mediterranean basin.

Effect of Deficit and Optimum Irrigation at Various Growth Stages on Yield Attributes, Yield and Economics of Summer Sesame

Aims: Sesame is grown in the country since antiquity. Sesame has high nutritional benefits and is utilized in numerous cooking styles everywhere globally. Sesame is called as the queen of oilseeds for the reason of its top notch polyunsaturated stable fats that limit oxidative rancidity and contains high oil content (up to 60%). Sesame is cultivated in summer in the North Telangana in turmeric and rice fallows. Low productivity in sesame is primarily due to rainfed planting on sub-marginal and marginal lands with poor management and low investment. To solve the upcoming challenges and in view of the improving yield and acquire higher returns, precised water management strategies need to be formulated. Hence the present investigation is proposed to study the effect of deficit and optimum irrigation at various growth stages on yield and economics of sesame crop grown in summer. Study Design: The experiment was laid out in a randomized complete block design. Methodology: A field experiment was conducted at Agricultural college, Polasa, Jagtial district during summer 2021 to study the effects of water deficit and optimal irrigation at various growth stages on yield and economics of summer sesame. The study is conducted with eight deficit and optimum irrigation treatments (T1 to T8) and replicated thrice. Results: The results of this experiment manifested that scheduling irrigation at vegetative, prebloom, flowering, capsule initiation and capsule filling stages (T8) registered highest yield attributes, yield and economic parametersviz., no of capsules plant-1 (45),capsule weight (0.32 g) and no of filled seeds capsule-1 (55), seed yield (1150 kg ha-1), stalk yield (1999 kg ha-1), gross returns (128499 ha-1), net returns (94391 ha-1) and benefit cost ratio (2.76).