scholarly journals The fatty acid composition of the mitochondrial membranes of platelets in chronic obstructive pulmonary disease.

2018 ◽  
Vol 96 (4) ◽  
pp. 343-347
Author(s):  
Y. K. Denisenko ◽  
T. P. Novgorodtseva ◽  
T. I. Vitkina ◽  
M. V. Antonyuk ◽  
Nataliia V. Bocharova
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Fatty Acids ◽  
Mitochondrial Dysfunction ◽  
Chronic Bronchitis ◽  
Pulmonary Disease ◽  
Blood Cells ◽  
Transport Systems ◽  
Chronic Obstructive ◽  
Mitochondrial Membranes ◽  
Obstructive Pulmonary Disease

Objective: to study the composition of fatty acids (FA) and mitochondrial membrane potential (MMР) of blood cells in patients with chronic bronchitis (CB) and chronic obstructive pulmonary disease (COPD); to establish the role of the Mitochondrial dysfunction in the formation of respiratory diseases. Material and methods. The study involved 26 patients with chronic bronchitis, 27 - with mild COPD, 21 - with moderate COPD steady flow, 29 healthy people. MMР leukocytes was assessed by flow cytometry. The composition of the FA mitochondrial membranes was studied by gas-liquid chromatography. Results and discussion. With the worsening of the disease (moderate COPD) increases the number of white blood cells with reduced MMP, appears deficit of the saturated, monoenic (14: 0, 16: 0, 18: 0) and n-3 polyunsaturated fatty acids (18: 3n-3 20: 5n-3, 22: 5n-3, 22: 6n-3) in the membrane of the mitochondria. Identified imbalance in the composition of fatty acids verifies the changes in the physicochemical properties of subcellular membrane malfunction membrane transport systems, enzymes and receptors, processes of oxidative phosphorylation. Conclusion: The imbalance in the composition of the LCD mitochondrial membranes lead to the development of mitochondrial dysfunction and the formation of cell hypoxia.

scholarly journals Development of secondary mitochondrial dysfunction of mononuclear blood leukocytes in patients with chronic obstructive pulmonary disease and chronic bronchitis

2018 ◽  
Vol 99 (5) ◽  
pp. 741-747
Author(s):  
E S Bel’skikh ◽  
O M Uryas’ev ◽  
V I Zvyagina ◽  
S V Faletrova
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Mitochondrial Dysfunction ◽  
Chronic Bronchitis ◽  
Pulmonary Disease ◽  
Oxidative Modification ◽  
Mononuclear Leukocytes ◽  
Chronic Obstructive ◽  
Adaptive Potential ◽  
Obstructive Pulmonary Disease ◽  
Oxidative Modification Of Proteins

Aim. To study the indicators of energy metabolism and oxidative stress in mononuclear leukocytes of peripheral blood and to assess the possibility of mitochondrial dysfunction development in chronic obstructive pulmonary disease and chronic bronchitis. Methods. The study included 50 patients aged 40 to 75 years with chronic obstructive pulmonary disease (COPD) or chronic bronchitis. The first group included 13 patients with chronic bronchitis. In accordance with the GOLD spirometric classification, the second and third groups included patients with COPD of moderate severity (COPD 2) (n=17) and severe COPD (COPD 3) (n=20) respectively. In the isolated mononuclear leukocytes, the activity of superoxide dismutase (SOD), succinate dehydrogenase (SDH) and concentration of succinate were determined, a complex evaluation of oxidative modification of proteins was performed. Results. Patients with chronic bronchitis compared to patients with COPD 2 and COPD 3 were found to have in mononuclear leukocytes higher activity of SOD by 3.38 times (p=0.0025) and 3.15 times (p=0.0058), higher activity of SDH by 4.55 times (p=0.0281) and 2.5 times (p=0.0263) and higher succinate concentration by 2.05 (p=0.0133) and 1.89 (p=0.005) times respectively. The level of spontaneously oxidized modified proteins in the group of patients with chronic bronchitis decreased by 2.45 (p=0.0176) and 2.94 (p=0.0168) times compared to the patients of groups 2 and 3, respectively There was a decrease in the reserve-adaptive potential of oxidative modification of proteins in COPD in the form of an increase of the ratio of spontaneously oxidized-modified proteins to metal-induced oxidized proteins by 1.58 times (p=0.0301) between groups 1 and 2, and by 1.44 times between groups 2 and 3 (p=0.0446). Conclusion. In mononuclear leukocytes of COPD patients, secondary mitochondrial dysfunction is observed accompanied by significant oxidative damage of lymphocytes and monocytes. Patients with severe COPD compared to patients with COPD of moderate severity have less reserve-adaptive potential for the oxidative modification of mononuclear leukocyte proteins, which probably reflects a more severe course of the disease.

MITOCHONDRIAL DYSFUNCTION IN CHRONIC OBSTRUCTIVE PULMONARY DISEASE

2016 ◽  
Vol 1 (60) ◽  
pp. 28-33
Author(s):  
Антонюк ◽  
Marina Antonyuk ◽  
Виткина ◽  
Tatyana Vitkina ◽  
Новгородцева ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Mitochondrial Dysfunction ◽  
Blood Cells ◽  
White Blood Cells ◽  
Functional Condition ◽  
Chronic Obstructive ◽  
Gas Liquid Chromatography ◽  
Mitochondrial Membranes ◽  
Obstructive Pulmonary Disease ◽  
Cell Hypoxia

The aim is to study structural and functional condition of cell mitochondrial apparatus for evaluating the mitochondrial membrane potential (MMР) and the fatty acids (FA) composition of mitochondrial membranes of blood cells in patients with COPD; to establish the role of mitochondrial dysfunction in the mechanism of COPD. The study involved 27 patients with mild COPD, 21 with moderate COPD of a stable course, and 20 healthy people. MMР of leukocytes was assessed with cytofluorimetry. The composition of FA of mitochondrial membranes was studied with gas-liquid chromatography. As a result of the study it was found out that the worsening of the disease (moderate COPD) leads to the increase of the number of white blood cells with reduced MMP and appearance of deficit of the saturated monoenic and n-3 polyunsaturated fatty acids in the membrane of mitochondria. The revealed changes in the structural and functional state of mitochondria show a violation in energetic activity, membrane permeability and transport of substances, which is a sign of the formation of mitochondrial dysfunction and cell hypoxia development at respiratory diseases.

scholarly journals Methylation of SERPINA1 gene promoter may predict chronic obstructive pulmonary disease in patients affected by acute coronary syndrome

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
John Charles Rotondo ◽  
Giorgio Aquila ◽  
Lucia Oton-Gonzalez ◽  
Rita Selvatici ◽  
Paola Rizzo ◽  
...  
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Acute Coronary Syndrome ◽  
Pulmonary Disease ◽  
Blood Cells ◽  
Gene Promoter ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Coronary Syndrome ◽  
Copd Patients ◽  
Potential Biomarker

Abstract Background Diagnostic biomarkers for detecting chronic obstructive pulmonary disease (COPD) in acute coronary syndrome (ACS) patients are not available. SERPINA1, coding for the most potent circulating anti-inflammatory protein in the lung, has been found to be differentially methylated in blood cells from COPD patients. This study aimed to investigate the methylation profile of SERPINA1 in blood cells from ACS patients, with (COPD+) or without COPD (COPD−). Methods Blood samples were from 115 ACS patients, including 30 COPD+ and 85 COPD− according to lung function phenotype, obtained with spirometry. DNA treated with sodium bisulfite was PCR-amplified at SERPINA1 promoter region. Methylation analysis was carried out by sequencing the PCR products. Lymphocytes count in ACS patients was recorded at hospital admission and discharge. Results SERPINA1 was hypermethylated in 24/30 (80%) COPD+ and 48/85 (56.5%) COPD− (p < 0.05). Interestingly, at hospital discharge, lymphocytes count was higher in COPD− patients carrying SERPINA1 hypermethylated (1.98 × 103 ± 0.6 cell/µl) than in COPD− carrying SERPINA1 hypomethylated (1.7 × 103 ± 0.48 cell/µl) (p < 0.05). Conclusions SERPINA1 is hypermethylated in blood cells from COPD+ patients. COPD− carrying SERPINA1 hypermethylated and high lymphocytes count may be at risk of COPD development. Therefore, SERPINA1 hypermethylation may represent a potential biomarker for predicting COPD development in ACS patients.

Dietary intake of fatty acids and its relationship with FEV1/FVC in patients with chronic obstructive pulmonary disease

2019 ◽  
Vol 29 ◽  
pp. 92-96
Author(s):  
Abril Jiménez-Cepeda ◽  
Giselle Dávila-Said ◽  
Arturo Orea-Tejeda ◽  
Dulce González-Islas ◽  
Marcela Elizondo-Montes ◽  
...  
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Fatty Acids ◽  
Dietary Intake ◽  
Pulmonary Disease ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease

scholarly journals EFFECT OF HAREETAKYADI YOGA IN CHRONIC OBSTRUCTIVE PULMONARY DISEASE – PRE TEST-POST TEST DESIGN

2021 ◽  
pp. 29-35
Author(s):  
Aswathy M ◽  
Radhakrishnan VN ◽  
Jithesh M
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Chronic Bronchitis ◽  
Pulmonary Disease ◽  
Study Drug ◽  
Lung Parenchyma ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Airway Narrowing ◽  
Post Test ◽  
Stage 1

Chronic Obstructive Pulmonary Disease (COPD) is a lung disease characterized by chronic obstruction of lung airflow that interferes with normal breathing, which is progressive and is not fully reversible. COPD includes chronic bronchitis and emphysema. Symptoms of COPD are well explained in Ayurveda in the context of Kasa and Swasa among which chronic bronchitis can be better correlated to Vataja and Kaphaja kasa and emphysema to Tamaka swasa. COPD is characterized by mucous hyper secretion, airway narrowing, fibrosis and destruction of lung parenchyma. Even though the existing conventional management is excellent, Ayurveda provides additional benefits such as improvement in quality of life. The study drug Hareetakyadi yoga is mentioned in Prakarana of kasa in Chikitsa manjari. The objective of the study was to evaluate the Effect of Hareetakyadi yoga in Chronic Obstructive Pulmonary Disease. The Study includes 10 subjects of age group 40-70 years of both gender, diagnosed clinically and as per investigations satisfying COPD, stage 1 & 2 as per GOLD criteria of spirometry attending department of Kayachikitsa, Government Ayurveda College, Thiruvananthapuram. A protocol from Deepana to Virechana were done as preparatory phase before administering drug. Hareetakyadi yoga having the properties of Kapha vata hara, Agni deepana and Rasayana property seems to be effective in the management of this disease.

Sign in / Sign up

Export Citation Format

Share Document