Liver ABCA1 Gene Expression in Male Rats: Effects of High-intensity Treadmill Running and Black Crataegus-pentaegyna (Siayh-Valik) Extraction

Objective: The aim of this study was to investigate the effect of 6 weeks of detraining after 12 weeks of high intensity interval training (HIIT) on the expression of AKT1 and mTORc1 genes in the left ventricle of wistar diabetic rats. Materials and Methods: Twenty-eight wistar male rats were selected as the study sample and were divided in four groups of healthy control, diabetic control, diabetic HIIT and diabetic HIIT + detraining. The HIIT period was 12 weeks and the detraining period was 6 weeks. Each session consisted of 30 minutes, which included running on a treadmill with one-minute repetitions and a two-minute active recovery between them. To measure AKT1 mRNA and mTORc1 mRNA by RT-Real time PCR, a single-step single step SYBR TAKARA kits from Takara Company was used according to the company's instruction. Results: HIIT caused a significant increase in AKT1 gene expression (P-value= 0.001). AKT1 decreased with detraining that was not significant (P-value= 0.34) but it was still significantly higher than before training (P-value= 0.017). HIIT caused a significant increase in mTORc1 gene expression (P-value= 0.001) and although it decreased with detraining (P-value= 0.15) and it was no significantly higher than before training (P-value= 0.19). Conclusion: HIIT led to increased expression of AKT1 and mTORc1 genes in type 2 diabetic rats, while also producing favorable changes in the cardiac structure of these rats. Also, 6 weeks of detraining did somewhat reduce these favorable changes.

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Effect of Eight-Week High Intensity Interval Training on Omentin-1 Gene Expression and Insulin-Resistance in Diabetic Male Rats

Annals of Applied Sport Science ◽

10.18869/acadpub.aassjournal.5.2.29 ◽

2017 ◽

Vol 5 (2) ◽

pp. 29-36 ◽

Cited By ~ 11

Author(s):

Mahdieh Alizadeh ◽

Mohammad Reza Asad ◽

Mohammad Faramarzi ◽

Roghayeh Afroundeh ◽

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...

Keyword(s):

Gene Expression ◽

Insulin Resistance ◽

High Intensity ◽

Interval Training ◽

Male Rats ◽

High Intensity Interval Training ◽

High Intensity Interval

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Comparison of the Effect of High Intensity Interval Training and Moderate Intensity Continuous Training on Gene Expression of Il-6 and Nf-Κb in Lung Tissue of Old and Young Male Rats

journal of ilam university of medical sciences ◽

10.29252/sjimu.27.5.54 ◽

2019 ◽

Vol 27 (5) ◽

pp. 54-64

Author(s):

Mandana Motiei Haghighi ◽

Rahman Soori ◽

Fatemeh Shabkhiz ◽

Siroos Choobine ◽

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...

Keyword(s):

Gene Expression ◽

Lung Tissue ◽

High Intensity ◽

Young Male ◽

Interval Training ◽

Male Rats ◽

Moderate Intensity ◽

High Intensity Interval Training ◽

Continuous Training ◽

High Intensity Interval

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The Effect of Six Weeks of High Intensity Interval Training on eNOS and PGC-1α Gene Expression in the Heart Tissue of Male Obese Rats

Jundishapur Journal of Health Sciences ◽

10.5812/jjhs.100280 ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Behnoush Ghadery ◽

Farshad Ghazalian ◽

Seyed Ali Hosseini ◽

Hossein Abed Natanzy ◽

Alireza Shamsoddini

Keyword(s):

Gene Expression ◽

High Intensity ◽

Interval Training ◽

Heart Tissue ◽

Male Rats ◽

High Intensity Interval Training ◽

Expression Levels ◽

Obese Rats ◽

High Intensity Interval ◽

Gene Expression Levels

Background: Nowadays, obesity can affect heart function and induced atherosclerosis, high blood pressure, and heart arrhythmia, which has become a major problem for global health. Objectives: The present study aimed to review the effect of six weeks of high-intensity interval training (HIIT) on gene expression levels of PGC-1α and eNOS in the heart tissue of obese male rats. Methods: In this experimental study, 14 high-fat diet (HFD)-induced obese rats were divided into two groups of control and training. Also, 7 rats were placed in a non-obese control group to investigate the effects of obesity on research variables. During six weeks, rats in the training group performed HIIT three days per week. After six weeks, all rats were sacrificed, and their heart tissue was removed to measure the PGC-1α and eNOS gene expression. We used one-way ANOVA with Tukey’s post hoc tests (P ≤ 0.05) for statistical analysis. Results: HFD significantly decreased PGC-1α (P = 0.04) and eNOS (P = 0.001) gene expression, but HIIT significantly increased PGC-1α (P = 001) and eNOS (P = 0.001) gene expression. Conclusions: HIIT seems to improve cardiac gene expression levels of PGC-1α and eNOS of male obese rats.

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