scholarly journals The Effect of Six Weeks of High Intensity Interval Training on eNOS and PGC-1α Gene Expression in the Heart Tissue of Male Obese Rats

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Behnoush Ghadery ◽  
Farshad Ghazalian ◽  
Seyed Ali Hosseini ◽  
Hossein Abed Natanzy ◽  
Alireza Shamsoddini
Keyword(s):  
Gene Expression ◽  
High Intensity ◽  
Interval Training ◽  
Heart Tissue ◽  
Male Rats ◽  
High Intensity Interval Training ◽  
Expression Levels ◽  
Obese Rats ◽  
High Intensity Interval ◽  
Gene Expression Levels

Background: Nowadays, obesity can affect heart function and induced atherosclerosis, high blood pressure, and heart arrhythmia, which has become a major problem for global health. Objectives: The present study aimed to review the effect of six weeks of high-intensity interval training (HIIT) on gene expression levels of PGC-1α and eNOS in the heart tissue of obese male rats. Methods: In this experimental study, 14 high-fat diet (HFD)-induced obese rats were divided into two groups of control and training. Also, 7 rats were placed in a non-obese control group to investigate the effects of obesity on research variables. During six weeks, rats in the training group performed HIIT three days per week. After six weeks, all rats were sacrificed, and their heart tissue was removed to measure the PGC-1α and eNOS gene expression. We used one-way ANOVA with Tukey’s post hoc tests (P ≤ 0.05) for statistical analysis. Results: HFD significantly decreased PGC-1α (P = 0.04) and eNOS (P = 0.001) gene expression, but HIIT significantly increased PGC-1α (P = 001) and eNOS (P = 0.001) gene expression. Conclusions: HIIT seems to improve cardiac gene expression levels of PGC-1α and eNOS of male obese rats.

scholarly journals The Effect of Detraining High Intensity Interval Training on the Expression of AKT1 and mTORc1 Genes in the Left Ventricle of Diabetic Rats

2020 ◽  
Author(s):  
Farzaneh Soltanipour jounaghani ◽  
Maghsoud Peeri ◽  
Mohammad-Ali Azarbayjani
Keyword(s):  
Gene Expression ◽  
Left Ventricle ◽  
High Intensity ◽  
Interval Training ◽  
Diabetic Rats ◽  
Single Step ◽  
Male Rats ◽  
P Value ◽  
High Intensity Interval Training ◽  
High Intensity Interval

Objective: The aim of this study was to investigate the effect of 6 weeks of detraining after 12 weeks of high intensity interval training (HIIT) on the expression of AKT1 and mTORc1 genes in the left ventricle of wistar diabetic rats. Materials and Methods: Twenty-eight wistar male rats were selected as the study sample and were divided in four groups of healthy control, diabetic control, diabetic HIIT and diabetic HIIT + detraining. The HIIT period was 12 weeks and the detraining period was 6 weeks. Each session consisted of 30 minutes, which included running on a treadmill with one-minute repetitions and a two-minute active recovery between them. To measure AKT1 mRNA and mTORc1 mRNA by RT-Real time PCR, a single-step single step SYBR TAKARA kits from Takara Company was used according to the company's instruction. Results: HIIT caused a significant increase in AKT1 gene expression (P-value= 0.001). AKT1 decreased with detraining that was not significant (P-value= 0.34) but it was still significantly higher than before training (P-value= 0.017). HIIT caused a significant increase in mTORc1 gene expression (P-value= 0.001) and although it decreased with detraining (P-value= 0.15) and it was no significantly higher than before training (P-value= 0.19). Conclusion: HIIT led to increased expression of AKT1 and mTORc1 genes in type 2 diabetic rats, while also producing favorable changes in the cardiac structure of these rats. Also, 6 weeks of detraining did somewhat reduce these favorable changes.

scholarly journals The Effect of High Intensity Interval Training and Herb Supplement on Heart IGF-1 Concentration in Male Rat

2018 ◽  
Vol 3 (1) ◽  
pp. 13-17
Author(s):  
Mohamad Ali Pirani ◽  
Maghsoud Peeri ◽  
Mohamad Ali Azarbayjani
Keyword(s):  
High Intensity ◽  
Training Session ◽  
Interval Training ◽  
Heart Tissue ◽  
Flaxseed Oil ◽  
Male Rats ◽  
Male Rat ◽  
High Intensity Interval Training ◽  
Training Groups ◽  
High Intensity Interval

Abstract Objectives: The objective of the present study was to determine the effect of 10 weeks of high intensity interval training and flaxseed oil supplement on heart IGF-1 concentration in male rats. Materials and methods: 20 adult male Wistar rats were randomly divided into saline, saline–training, supplementation, and supplementation–training groups. The training groups performed training (10 weeks, five sessions per week, 90–95% VO2 max) on a rodent treadmill. The supplementation groups also received flaxseed oil supplement (30 mg/kg). The rats were sacrificed five days after the last training session. The heart tissue was collected, and sent to the laboratory for evaluation. Results: Training increased the concentration of the heart IGF-1 (P=0.01). The concentration of heart IGF-1 was higher in the flaxseed oil-supplemented groups than the saline-treated groups. (P=0.003). The interaction between training and supplementation also led to a increase in heart IGF-1 concentration (P=0.001). Conclusion: The increase of heart IGF-1 after training and consumption suggest that training and flaxseed oil can help to improve cardiac function.

scholarly journals High-intensity interval training-induced hypertrophy in gastrocnemius muscle via improved IGF-I/Akt/FoxO and myostatin/Smad signaling pathways in rats

2020 ◽  
Vol 107 (2) ◽  
pp. 220-230 ◽  
Author(s):  
Soheil Biglari ◽  
Alireza Ghardashi Afousi ◽  
Farnoosh Mafi ◽  
Fatemeh Shabkhiz
Keyword(s):  
Signal Transduction ◽  
High Intensity ◽  
Muscle Hypertrophy ◽  
Gastrocnemius Muscle ◽  
Interval Training ◽  
Male Rats ◽  
Signal Transduction Pathways ◽  
High Intensity Interval Training ◽  
Igf I ◽  
High Intensity Interval

AbstractObjectiveIt has been shown that high-intensity interval training (HIIT) leads to skeletal muscle hypertrophy; however, its mechanisms of cellular and molecular regulation are still unclear. The purpose of this study was to investigate the effect of HIIT on muscle hypertrophy and major signal transduction pathways.Design12 male rats were randomly divided into two groups: control and HIIT. The exercise group performed 30-min HIIT in each session (5 × 4-min intervals running at 85–95% VO2max separated by 2-min active rest at 55–60% VO2max), 3 days/week for 8 weeks. Muscle fiber cross-sectional area (CSA) and the expression of signal transduction pathway proteins were determined in the gastrocnemius muscle.ResultsIn the HIIT group, the expression of IGF-I, IGF-IR Akt, p-Akt, AMPKα, p-AMPKα and follistatin increased significantly, whereas a significant decrease was observed in the expression of FoxO1, p-FoxO1, myostatin, ActRIIB, Smad2/3 and p-Smad2/3 (P < 0.05). However, there were no significant differences between the HIIT and control groups in the expression of mTOR, p-mTOR, P70S6K, and p-P70S6K (P > 0.05). In addition, CSA and gastrocnemius muscle weight increased significantly in the HIIT group (P < 0.05).ConclusionsHIIT induced muscle hypertrophy by improving IGF-I/Akt/FoxO and myostatin/Smad signal transduction pathways.

scholarly journals High-intensity interval training modulates retinal microvascular phenotype and DNA methylation of p66Shc gene: a randomized controlled trial (EXAMIN AGE)

2019 ◽  
Vol 41 (15) ◽  
pp. 1514-1519 ◽  
Author(s):  
Lukas Streese ◽  
Abdul Waheed Khan ◽  
Arne Deiseroth ◽  
Shafaat Hussain ◽  
Rosa Suades ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Dna Methylation ◽  
High Intensity ◽  
Interval Training ◽  
Retinal Vessel ◽  
High Intensity Interval Training ◽  
Age Related ◽  
High Intensity Interval ◽  
Retinal Arteriolar

Abstract Aims Impairments of retinal vessel diameter are associated with major adverse cardiovascular (CV) events. Promoter DNA methylation is a repressor of the mitochondrial adaptor p66Shc gene transcription, a key driver of ageing-induced reactive oxygen species. The study aimed to investigate whether high-intensity interval training (HIIT) affects retinal microvascular phenotype as well as p66Shc expression and oxidative stress in ageing subjects with increased CV risk from the EXAMIN AGE cohort. Methods and results Eighty-four sedentary subjects (mean age 59.4 ± 7.0 years) with ≥2 CV risk factors were randomized into either a 12-week HIIT or standard physical activity recommendations. Retinal arteriolar and venular diameters were measured by use of a retinal vessel analyser. As a marker of oxidative stress plasma 3-nitrotyrosine (3-NT) level was determined by ELISA. Gene expression of p66Shc and DNA methylation were assessed in mononuclear cells by RT-qPCR and methylated-DNA capture (MethylMiner Enrichment Kit) coupled with qPCR, respectively. High-intensity interval training reduced body mass index, fat mass, low-density lipoprotein and increased muscle mass, as well as maximal oxygen uptake (VO2max). Moreover, HIIT restored microvascular phenotype by inducing retinal arteriolar widening (pre: 175 ± 14 µm vs. post: 181 ± 13 µm, P = 0.001) and venular narrowing (pre: 222 ± 14 µm vs. post: 220 ± 14 µm, P = 0.007). After HIIT, restoration of p66Shc promoter methylation (P = 0.034) reduced p66Shc gene expression (P = 0.037) and, in turn, blunted 3-NT plasma levels (P = 0.002). Conclusion High-intensity interval training rescues microvascular dysfunction in ageing subjects at increased CV risk. Exercise-induced reprogramming of DNA methylation of p66Shc gene may represent a putative mechanistic link whereby exercise protects against age-related oxidative stress. Clinical trial registration  ClinicalTrials.gov: NCT02796976 (https://clinicaltrials.gov/ct2/show/NCT02796976).

Sign in / Sign up

Export Citation Format

Share Document