scholarly journals Molecular Mechanisms Involved in Intrarenal Renin-Angiotensin and Alternative Pathways in Diabetic Nephropathy - A Review

2021 ◽  
Vol 17 (1) ◽  
pp. 1-10
Author(s):  
Elham Bahreini ◽  
Yousef Rezaei-Chianeh ◽  
Mohsen Nabi-Afjadi
Keyword(s):  
Diabetic Nephropathy ◽  
Molecular Mechanisms ◽  
Renin Angiotensin ◽  
Alternative Pathways

Maximum doses of renin-angiotensin axis blockers in diabetic nephropathy?

2021 ◽  
Author(s):  
Nicolás Roberto Robles Pérez-Monteoliva ◽  
José Carlos Arévalo Lorido
Keyword(s):  
Diabetic Nephropathy ◽  
Renin Angiotensin

scholarly journals Investigation of the Mechanism of Complement System in Diabetic Nephropathy via Bioinformatics Analysis

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Bojun Xu ◽  
Lei Wang ◽  
Huakui Zhan ◽  
Liangbin Zhao ◽  
Yuehan Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Diabetic Nephropathy ◽  
Protein Interactions ◽  
Molecular Mechanisms ◽  
Bioinformatics Analysis ◽  
Topological Analysis ◽  
Gene Expression Omnibus ◽  
Complement Cascade ◽  
Hub Genes ◽  
Novel Biomarkers

Objectives. Diabetic nephropathy (DN) is a major cause of end-stage renal disease (ESRD) throughout the world, and the identification of novel biomarkers via bioinformatics analysis could provide research foundation for future experimental verification and large-group cohort in DN models and patients. Methods. GSE30528, GSE47183, and GSE104948 were downloaded from Gene Expression Omnibus (GEO) database to find differentially expressed genes (DEGs). The difference of gene expression between normal renal tissues and DN renal tissues was firstly screened by GEO2R. Then, the protein-protein interactions (PPIs) of DEGs were performed by STRING database, the result was integrated and visualized via applying Cytoscape software, and the hub genes in this PPI network were selected by MCODE and topological analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to determine the molecular mechanisms of DEGs involved in the progression of DN. Finally, the Nephroseq v5 online platform was used to explore the correlation between hub genes and clinical features of DN. Results. There were 64 DEGs, and 32 hub genes were identified, enriched pathways of hub genes involved in several functions and expression pathways, such as complement binding, extracellular matrix structural constituent, complement cascade related pathways, and ECM proteoglycans. The correlation analysis and subgroup analysis of 7 complement cascade-related hub genes and the clinical characteristics of DN showed that C1QA, C1QB, C3, CFB, ITGB2, VSIG4, and CLU may participate in the development of DN. Conclusions. We confirmed that the complement cascade-related hub genes may be the novel biomarkers for DN early diagnosis and targeted treatment.

Renin-angiotensin-aldosterone system blockers for diabetic nephropathy

Vrach ◽  
2021 ◽  
Vol 32 (10) ◽  
pp. 16-24
Author(s):  
A. Bagriy ◽  
M. Khomenko ◽  
E. Mikhailichenko
Keyword(s):  
Diabetic Nephropathy ◽  
Renin Angiotensin Aldosterone System ◽  
Renin Angiotensin

scholarly journals SP300CIRCULATING TNF RECEPTOR LEVELS AND RENAL DISEASE PROGRESSION IN PATIENTS WITH TYPE 2 DIABETIC NEPHROPATHY AND BLOCKADE OF THE RENIN ANGIOTENSIN SYSTEM

2015 ◽  
Vol 30 (suppl_3) ◽  
pp. iii478-iii479
Author(s):  
Gema Maria Fernandez Juarez ◽  
Javier Villacorta Pérez ◽  
Ernesto Martinez Martinez ◽  
Victoria Cachofeiro ◽  
Ana Tato ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Renal Disease ◽  
Disease Progression ◽  
Renin Angiotensin System ◽  
Tnf Receptor ◽  
Renal Disease Progression ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Type 2 Diabetic

scholarly journals Long Non-Coding RNA NEAT1 Regulates Pyroptosis in Diabetic Nephropathy via Mediating the miR-34c/NLRP3 Axis

2020 ◽  
Vol 45 (4) ◽  
pp. 589-602 ◽  
Author(s):  
Jin-Feng Zhan ◽  
Hong-Wei Huang ◽  
Chong Huang ◽  
Li-Li Hu ◽  
Wen-Wei Xu
Keyword(s):  
Diabetic Nephropathy ◽  
Target Gene ◽  
Molecular Mechanisms ◽  
Mesangial Cells ◽  
Receptor Protein ◽  
Luciferase Reporter ◽  
Glomerular Mesangial Cells ◽  
Non Coding Rna ◽  
Vitro Model

Introduction: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and is considered to be a sterile inflammatory disease. Increasing evidence suggest that pyroptosis and subsequent inflammatory response play a key role in the pathogenesis of DN. However, the underlying cellular and molecular mechanisms responsible for pyroptosis in DN are largely unknown. Methods: The rat models of DN were successfully established by single 65 mg/kg streptozotocin treatment. Glomerular mesangial cells were exposed to 30 mmol/L high glucose media for 48 h to mimic the DN environment in vitro. Gene and protein expressions were determined by quantitative real-time PCR and Western blot. Cell viability and pyroptosis were measured by MTT assay and flow cytometry analysis, respectively. The relationship between lncRNA NEAT1, miR-34c, and Nod-like receptor protein-3 (NLRP3) was confirmed by luciferase reporter assay. Results: We found that upregulation of NEAT1 was associated with the increase of pyroptosis in DN models. miR-34c, as a target gene of NEAT1, mediated the effect of NEAT1 on pyroptosis in DN by regulating the expression of NLRP3 as well as the expressions of caspase-1 and interleukin-1β. Either miR-34c inhibition or NLRP3 overexpression could reverse the accentuation of pyroptosis and inflammation by sh-NEAT1 transfection in the in vitro model of DN. Conclusions: Our findings suggested NEAT1 and its target gene miR-34c regulated cell pyroptosis via mediating NLRP3 in DN, providing new insights into understanding the molecular mechanisms of pyroptosis in the pathogenesis of DN.

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