Comparative SEM Study on the Effect of Acid Etching With Tetracycline HCl or Citric Acid on Instrumented Periodontally-Involved Human Root Surfaces

1993 ◽  
Vol 64 (8) ◽  
pp. 689-693 ◽  
Author(s):  
Thomas A. Lafferty ◽  
Marlin E. Gher ◽  
Jonathan L. Gray
Keyword(s):  
Citric Acid ◽  
Acid Etching ◽  
Sem Study ◽  
Root Surfaces ◽  
Tetracycline Hcl

The Effect of EDTA, Tetracycline-HCl, and Citric Acid on Diseased Root Surfaces: The SEM Study

1999 ◽  
Vol 29 (3) ◽  
pp. 561
Author(s):  
Seong Hee Ahn ◽  
Jung Kiu Chai ◽  
Chong Kwan Kim ◽  
Kyoo Sung Cho
Keyword(s):  
Citric Acid ◽  
Sem Study ◽  
Diseased Root ◽  
Root Surfaces ◽  
Tetracycline Hcl

scholarly journals Effect of Bioconditioning on Surface Characteristics of Periodontally-affected Roots of Diabetic Patients

2014 ◽  
Vol 5 (2) ◽  
pp. 81-86
Author(s):  
Hossam A Eid ◽  
Khalid M Abdelaziz ◽  
Refaat A Eid
Keyword(s):  
Citric Acid ◽  
Chronic Periodontitis ◽  
Negative Impact ◽  
Surface Characteristics ◽  
Diabetic Patients ◽  
Type Ii ◽  
Sem Images ◽  
Root Surfaces ◽  
Tetracycline Hcl

ABSTRACT Objective Advanced glycosylated end products (AGEs) in type II diabetic patients are usually precipitated on the periodontallyaffected root surfaces. The presence of periodontopathic microbes, at the same time, may also add a negative impact on the prognosis of the regenerative periodontal surgery. This in vitro study aimed to evaluate the effect of chemical conditioning on surface characteristics of periodontally-affected roots of diabetic patients. Methods Three groups (n = 25) of freshly-extracted teeth were collected from the outpatient clinics, College of Dentistry, King Khalid University. In group 1, teeth were collected from healthy individuals for orthodontic purpose. Teeth of group 2 were collected from healthy patients with chronic periodontitis, while those of group 3 were collected from diabetic patients with chronic periodontitis. Roots of the collected teeth were examined using the scanning electron microscope (SEM) before and after mechanical surface planning and chemical treatment using normal saline, EDTA gel, Tetracycline HCL (TC) or citric acid (CA) each for 4 minutes (n = 5 from each group). Results Interpretation of SEM images revealed undesirable etching effect of the chemicals used on the surfaces of healthy roots. Although EDTA showed an effective cleanse of the smear debris, it seemed to have no power on surface cuticles existed on roots of diabetic individuals. Tetracycline HCl provided acceptable conditioning of periodontally-affected root surfaces. Citric acid showed a powerful removal of both smear debris and cuticle layers off the periodontally-affected roots of diabetic individuals. Conclusion Topical application of EDTA, TC or CA shows sensible effect on the periodontally affected root surfaces. However, each of these chemicals exhibits different conditioning power. Citric acid is a promising agent to biomodify the periodontally-affected root surfaces of diabetic patients. Clinical relevance Citric acid is an acceptable biomodifier for the periodontally-affected root surfaces. This approach may improve the prognosis of periodontal therapies especially in type II diabetic patients. How to cite this article Abdelaziz KM, Eid HA, Eid RA. Effect of Bioconditioning on Surface Characteristics of Periodontally- affected Roots of Diabetic Patients. World J Dent 2014;5(2):81-86.

The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study

2006 ◽  
Vol 7 (1) ◽  
pp. 35-43 ◽  
Author(s):  
R. Viswa Chandra ◽  
Ganesh Chandra Jagetia ◽  
K. Mahalinga Bhat
Keyword(s):  
Citric Acid ◽  
Periodontal Ligament ◽  
Chinese Hamster ◽  
Lung Fibroblasts ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts ◽  
Root Surfaces ◽  
Tetracycline Hcl

Abstract Objective The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Materials and Methods Commercially availableV79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 μg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). Results The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). Conclusion The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration. Citation Chandra RV, Jagetia GC, Bhat KM. The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study. J Contemp Dent Pract 2006 February;(7)1:044-059.

The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study

2006 ◽  
Vol 7 (1) ◽  
pp. 44-59 ◽  
Author(s):  
R. Viswa Chandra ◽  
Ganesh Chandra Jagetia ◽  
K. Mahalinga Bhat
Keyword(s):  
Citric Acid ◽  
Periodontal Ligament ◽  
Chinese Hamster ◽  
Lung Fibroblasts ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts ◽  
Root Surfaces ◽  
Tetracycline Hcl

Abstract Objective The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Materials and Methods Commercially availableV79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 μg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). Results The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). Conclusion The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration. Citation Chandra RV, Jagetia GC, Bhat KM. The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study. J Contemp Dent Pract 2006 February;(7)1:044-059.

scholarly journals Effects of Citric Acid and EDTA on Periodontally Involved Root Surfaces: A SEM Study

2012 ◽  
Vol 13 (4) ◽  
pp. 446-451 ◽  
Author(s):  
Sunkara Shree Ramalinga Prasad ◽  
Chitturi Radharani ◽  
Siddhartha Varma ◽  
SV Kiran Kumar ◽  
Soumya Sinha ◽  
...  
Keyword(s):  
Citric Acid ◽  
Smear Layer ◽  
Control Group ◽  
Dentinal Tubule ◽  
Root Planing ◽  
Group I ◽  
Sem Study ◽  
The Mean ◽  
Hand Instrumentation ◽  
Root Surfaces

ABSTRACT Aim Root debridement by scaling and root planing may not completely remove contaminated cementum particularly in more apical areas. The smear layer formed during root planing inhibits cell migration and attachment leading to impaired marginal periodontal healing. The present study was done to compare the morphological effects of root surface demineralization using citric acid and EDTA as root-conditioning agents. Materials and methods Thirty fragments of human dental roots previously exposed to periodontal disease were scaled and randomly divided into the following groups of treatment: Group I: Hand instrumentation and conditioning with normal saline for 5 minutes as control; group II: Hand instrumentation and conditioning with citric acid (pH 1) for 5 minutes and group III: Hand instrumentation and conditioning with EDTA (pH-7.4) for 5 minutes. Scanning electron microscopy was used to check for the presence of residual smear layer and for measuring the number and area of exposed dentin tubules. Results The mean efficacy of smear layer removal was compared between groups I and II, groups I and III and groups II and III, it was found to be statistically significant at 5%. When the mean diameter of the dentinal tubules and mean total surface area occupied by the dentinal tubule orifices was compared between groups II and III it was found to be statistically significant at 1% level of significance. Conclusion Citric acid causes greater degree of morphological alterations than EDTA and is considered to be a better rootconditioning agent. However, the use of EDTA cannot be ruled out. Clinical significance This study supports the hypothesis that demineralizing agents can be used as an adjunct to the periodontal treatment aiming at restoring the biocompatibility of roots and helps in choosing an appropriate agent for root conditioning. How to cite this article Prasad SSR, Radharani C, Varma S, Kumar SVK, Sinha S, Bijle MNA. Effects of Citric Acid and EDTA on Periodontally Involved Root Surfaces: A SEM study. J Contemp Dent Pract 2012;13(4):446-451.

scholarly journals Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study

2014 ◽  
Vol 18 (1) ◽  
pp. 32 ◽  
Author(s):  
GurparkashSingh Chahal ◽  
Kamalpreet Chhina ◽  
Vipin Chhabra ◽  
Rakhi Bhatnagar ◽  
Amna Chahal
Keyword(s):  
Citric Acid ◽  
Sem Study ◽  
Root Surfaces

Retention of Antimicrobial Activity by Human Root Surfaces after in Situ Subgingival Irrigation with Tetracycline HCl or Chlorhexidine

1993 ◽  
Vol 64 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Ayala Stabholz ◽  
James Kettering ◽  
Raydolfo Aprecio ◽  
Grenith Zimmerman ◽  
Pamela J. Baker ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Root Surfaces ◽  
Tetracycline Hcl

Etching Patterns of Sodium Hypochlorite Pretreated Hypocalcified Amelogenesis Imperfecta Primary Molars: SEM Study

2019 ◽  
Vol 43 (4) ◽  
pp. 257-262
Author(s):  
Amel Mahmoud Ahmed ◽  
Dina Nagy ◽  
Mona Abdallah Elkateb
Keyword(s):  
Phosphoric Acid ◽  
Sodium Hypochlorite ◽  
Amelogenesis Imperfecta ◽  
Acid Etching ◽  
Primary Molars ◽  
Control Group ◽  
Type I ◽  
Etching Pattern ◽  
Sem Study ◽  
Group Control Group

Aim: To investigate the etching patterns of hypocalcified amelogenesis imperfecta (AI) in primary molars pretreated with 5.25% NaOCl prior to phosphoric acid application using scanning electron microscopy (SEM). Study design: Ten hypocalcified AI primary molars were collected, sectioned longitudinally into 2 parts and allocated into two groups of ten specimens each. The enamel surface in the first group (control group) was etched using 37% phosphoric acid gel for 15 seconds; while in the second group (study group), it was pretreated using 5.25 sodium hypochlorite(NaOCl) for 60 seconds prior to acid etching. Each specimen was examined at 16 different sites, and evaluated for the etching pattern (types I, II, and III) distribution using SEM. A total of 320 microphotographs at 1,500 magnification were obtained using Auto-Cad 2007 software. Results: The etching pattern with phosphoric acid was not uniform with predominance of type III etching (65.63%), while the pretreated enamel surfaces showed a significant increase in type I and II(82.5%) etching patterns (P<0.001). Conclusion: Treatment of primary teeth affected by hypocalcified AI using 5.25% NaOCl prior to phosphoric acid etching significantly improves the etching pattern which is required for good resin bonding.

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