scholarly journals Polymorphic variation and phylogeny of the myostatin gene in wild Punjab Urial (Ovis vignei punjabiensis)

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Asad Ullah Ghalib
Keyword(s):  
Polymorphic Variation ◽  
Myostatin Gene

Production of myostatin gene knockout Wuzhishan miniature pig fibroblasts with zinc-finger nucleases

2013 ◽  
Vol 35 (6) ◽  
pp. 778-785
Author(s):  
Sui-Zhong CAO ◽  
Cheng-He YUE ◽  
Xi-Rui LI ◽  
Chong FENG ◽  
Chuan LONG ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Gene Knockout ◽  
Zinc Finger Nucleases ◽  
Miniature Pig ◽  
Myostatin Gene

scholarly journals MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 423
Author(s):  
Genxi Zhang ◽  
Mingliang He ◽  
Pengfei Wu ◽  
Xinchao Zhang ◽  
Kaizhi Zhou ◽  
...  
Keyword(s):  
Muscle Growth ◽  
Luciferase Reporter ◽  
Myoblast Differentiation ◽  
Regulation Mechanism ◽  
Rna Seq ◽  
Myostatin Gene ◽  
Primary Myoblasts ◽  
Chicken Muscle ◽  
Proliferation And Differentiation ◽  
Myoblast Proliferation

microRNAs play an important role in the growth and development of chicken embryos, including the regulation of skeletal muscle genesis, myoblast proliferation, differentiation, and apoptosis. Our previous RNA-seq studies showed that microRNA-27b-3p (miR-27b-3p) might play an important role in regulating the proliferation and differentiation of chicken primary myoblasts (CPMs). However, the mechanism of miR-27b-3p regulating the proliferation and differentiation of CPMs is still unclear. In this study, the results showed that miR-27b-3p significantly promoted the proliferation of CPMs and inhibited the differentiation of CPMs. Then, myostatin (MSTN) was confirmed to be the target gene of miR-27b-3p by double luciferase reporter assay, RT-qPCR, and Western blot. By overexpressing and interfering with MSTN expression in CPMs, the results showed that overexpression of MSTN significantly inhibited the proliferation and differentiation of CPMs. In contrast, interference of MSTN expression had the opposite effect. This study showed that miR-27b-3p could promote the proliferation of CPMs by targeting MSTN. Interestingly, both miR-27b-3p and MSTN can inhibit the differentiation of CPMs. These results provide a theoretical basis for further understanding the function of miR-27b-3p in chicken and revealing its regulation mechanism on chicken muscle growth.

scholarly journals Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1447
Author(s):  
Ishaku L. Haruna ◽  
Yunhai Li ◽  
Ugonna J. Ekegbu ◽  
Hamed Amirpour-Najafabadi ◽  
Huitong Zhou ◽  
...  
Keyword(s):  
Fatty Acid ◽  
New Zealand ◽  
Nucleotide Sequence ◽  
Milk Fat ◽  
Linear Mixed Effect Model ◽  
Polymorphism Analysis ◽  
Mixed Effect ◽  
Myostatin Gene ◽  
Holstein Friesian ◽  
Nucleotide Sequence Variation

The myostatin gene (MSTN), which encodes the protein myostatin, is pleiotropic, and its expression has been associated with both increased and decreased adipogenesis and increased skeletal muscle mass in animals. In this study, the polymerase chain reaction, coupled with single strand conformation polymorphism analysis, was utilized to reveal nucleotide sequence variation in bovine MSTN in 410 New Zealand (NZ) Holstein-Friesian × Jersey (HF × J)-cross cows. These cows ranged from 3 to 9 years of age and over the time studied, produced an average 22.53 ± 2.18 L of milk per day, with an average milk fat content of 4.94 ± 0.17% and average milk protein content of 4.03 ± 0.10%. Analysis of a 406-bp amplicon from the intron 1 region, revealed five nucleotide sequence variants (A–E) that contained seven nucleotide substitutions. Using general linear mixed-effect model analyses the AD genotype was associated with reduced C10:0, C12:0, and C12:1 levels when compared to levels in cows with the AA genotype. These associations in NZ HF × J cross cows are novel, and they suggest that this variation in bovine MSTN could be explored for increasing the amount of milk unsaturated fatty acid and decreasing the amount of saturated fatty acid.

scholarly journals PD-1 gene polymorphic variation is linked with first symptom of disease and severity of relapsing-remitting form of MS

2017 ◽  
Vol 305 ◽  
pp. 115-127 ◽  
Author(s):  
Edyta Pawlak-Adamska ◽  
Oskar Nowak ◽  
Lidia Karabon ◽  
Anna Pokryszko-Dragan ◽  
Anna Partyka ◽  
...  
Keyword(s):  
Polymorphic Variation ◽  
Relapsing Remitting

scholarly journals Polymorphic Variation in Cytochrome Oxidase Subunit Genes

2010 ◽  
Vol 21 (1) ◽  
pp. 141-154 ◽  
Author(s):  
Jianghua Lu ◽  
Kaixuan Wang ◽  
Mariana Rodova ◽  
Raquel Esteves ◽  
Diana Berry ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Polymorphic Variation ◽  
Cytochrome Oxidase Subunit ◽  
Oxidase Subunit

Effects of equine myostatin (MSTN) genotype variation on transcriptional responses in Thoroughbred skeletal muscle

2019 ◽  
Vol 15 (5) ◽  
pp. 327-338
Author(s):  
K. Bryan ◽  
L.M. Katz ◽  
E.W. Hill
Keyword(s):  
Skeletal Muscle ◽  
Skeletal Muscle Fibre ◽  
P Value ◽  
Transcriptional Responses ◽  
Myostatin Gene ◽  
Muscle Gene Expression ◽  
Gene Differential Expression ◽  
Sine Insertion ◽  
Significant Enrichment ◽  
Muscle Gene

Myostatin gene (MSTN) variation influences distance aptitude in Thoroughbreds as a consequence of functional physiological effects including skeletal muscle fibre type and muscle hypertrophy variation. A promotor region short interspersed nuclear element (SINE) insertion, tagged by SNP g.66493737-C, alters MSTN mRNA expression. We tested the hypothesis that skeletal muscle gene expression varies among MSTN genotypes due to differential up- or down-stream gene signalling pathways that may be influenced by exercise and training and consequently contribute to variation in exercise phenotypes. Skeletal muscle biopsies were collected from Thoroughbreds previously genotyped for MSTN (n=35 CC, n=50 CT, n=9 TT) at three different time-points: untrained at rest (UR), untrained after exercise (UE) and trained at rest (TR). Gene differential expression (DE) was determined from RNAseq data using DESeq2 (Benjamini-Hochberg P-value <0.05). Functional over-representation analysis was performed in DAVID. In UR samples, one, nine and 47 genes were DE between CC vs CT, CT vs TT and C:C vs TT, respectively. The OSGEPL1 gene, located <250 Kb proximal to MSTN, was DE among all cohorts. Six genes were DE in UE between CC vs TT including OSGEPL1, FGF10 and COQ8A. There was significant enrichment for GO categories related to mitochondria in TR. Comparison of the exercise response (UR vs UE) revealed patterns of expression that were opposing; i.e. CHRNG was 0.857 log2FC in the TT cohort but 2.055 log2FC in the CC cohort. Genes located in proximity to MSTN and involved in mitochondrial function were most significantly different among genotype cohorts. Patterns of DE among genotypes suggests gene-regulated influence on the phenotype. Understanding these patterns may assist genotype-guided training strategies.

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