scholarly journals A Study on the Role of Cartridge Based Nucleic Acid Amplification Test (CBNAAT) for Diagnosing Pediatric Tuberculosis in a Tertiary Care Hospital in Eastern India

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Kumar Anshu Suman Das
Keyword(s):  
Nucleic Acid ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Care Hospital ◽  
Eastern India ◽  
Pediatric Tuberculosis

Cartridge-based nucleic acid amplification test: a novel rapid diagnostic tool to study the burden of tuberculosis from a tertiary care hospital

2019 ◽  
Vol 49 (4) ◽  
pp. 274-281 ◽  
Author(s):  
Bineeta Kashyap ◽  
Nisha Goyal ◽  
Puneeta Hyanki ◽  
NP Singh ◽  
Ashwani Khanna
Keyword(s):  
Nucleic Acid ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Polymerase Chain Reaction Test ◽  
Rifampicin Resistance ◽  
People Living With Hiv ◽  
Care Hospital ◽  
Extra Pulmonary

Despite efforts to limit the morbidity and mortality from tuberculosis (TB), it continues to be an important cause of death. There is an urgent need for a diagnostic test that accurately and quickly diagnoses TB, especially if it is also a near-point-of-care test. The GeneXpert polymerase chain reaction test (known in India as CBNAAT [cartridge-based nucleic acid amplification test] and is capable of diagnosing TB and rifampicin resistance within 2 h) is a promising tool. The duration of our study was two years and was carried out in the DOTS centre of a tertiary care hospital in India. A total of 5449 samples were processed using CBNAAT. Of the total samples tested, 2068 were extra-pulmonary. The following information was collected: number of extra-pulmonary samples processed; number of Mycobacterium tuberculosis ( M. tuberculosis)-positive samples; patterns of rifampicin sensitivity; number of people living with HIV (PLHIV); and number of children. Of the samples, 62.1% were from suspected pulmonary TB patients. Out of the total samples tested using CBNAAT, 21.8% were positive for M. tuberculosis. Rifampicin resistance was seen in 9.2%, 8.5% and 10.3% of the total, pulmonary and extra-pulmonary samples, respectively, in M. tuberculosis-positive samples. Overall, 36.9% samples were from the paediatric population and 5.7% belonged to PLHIV. Rifampicin resistance was seen in 8.8% and 8.3% of the M. tuberculosis-positive paediatric and PLHIV samples, respectively.

ROLE OF CT SCAN OF PARANASAL AIR SINUSES FOR DIAGNOSTIC EVALUATION OF SINONASAL PATHOLOGY IN A TERTIARY CARE HOSPITAL IN EASTERN INDIA.

2021 ◽  
pp. 17-19
Author(s):  
Sk. Jishan Shine Alam ◽  
Swadha Priya Basu ◽  
Sayantan Roy ◽  
Md. Sajid
Keyword(s):  
Predictive Value ◽  
Chronic Sinusitis ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Sinus Surgery ◽  
Fungal Sinusitis ◽  
Care Hospital ◽  
Eastern India ◽  
Medical College

This study aims to evaluate the sensitivity and specicity of CT in diagnosis of sinonasal diseases along with the spectrum of Paranasal sinus diseases oncomputerized tomography (CT) and correlate their clinical and histopathological ndings. 40patients who have undergone endoscopic sinus surgery, their relevant histopathological and surgical reports are included in Radiodiagnosis department of Nil Ratan Sircar Medical College. Chronic sinusitis has 100% sensitivity,94% specicity, 96% positive predictive value (PPV) and 100% negative predictive value (NPV). For fungal sinusitis the sensitivity was 66%,specicity was 97.4%, PPV 66% and NPV 97.4%. Polyps have sensitivity of 90.9%, specicity of 94%, PPV 90% and NPV 100% Benign neoplasms have sensitivity of 100%, specicity of 100%, PPV 100% and NPV 100%. CT may be used as gold standardimaging modality for evaluating the PNS diseases.

scholarly journals Study of extrapulmonary tuberculosis in tertiary care hospital children with reference to cartridge based nucleic acid amplification test

2021 ◽  
Vol 8 (12) ◽  
pp. 1947
Author(s):  
Chirag Shah ◽  
Shantanu Jain ◽  
Pathik Patel
Keyword(s):  
Nucleic Acid ◽  
Extrapulmonary Tuberculosis ◽  
Tertiary Care ◽  
High Sensitivity ◽  
Tuberculosis Patient ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Care Hospital ◽  
Gastric Aspirate ◽  
Lymph Node Tuberculosis

Background: Tuberculosis is an infectious disease cause by the mycobacterium tuberculosis which typically involve lung but can affect other site called extrapulmonary tuberculosis (EPTB). In EPTB lymph node tuberculosis is most common and most severe is tuberculous meningitis. Cartridge based nucleic acid amplification test (CBNAAT) have high sensitivity and perform both respiratory and non-respiratory specimen. The aim of this retrospective study is to find out the proportion of extra pulmonary tuberculosis in hospitalized patient and determine the association of CBNAAT in diagnosis EPTB and Rifampicin resistance.Methods: This is the retrospective observational study done at Civil Hospital Ahmedabad in between October 2017 to October 2019 among the admitted patient age 1 month to 12 year diagnosed cases of EPTB according to RNTCP guidelines. Data collection done by medical records as indoor case sheets and investigation data from laboratory department.Results: Incidence of EPTB among the hospitalized children is 1.95% and among total tuberculosis patient is 42.48%. Among 191 suspected EPTB cases 59 confirmed indicate sensitivity of 37.3% and no false positive cases indicate 100% positive predictive value. Sensitivity of CBNAAT was highest in gastric aspirate followed by CSF in present study.Conclusions: Tuberculosis is more common in male child less than 5-year-old with frequent risk factor is rural residential area, lower socioeconomic class and contact with active cases. In my study is CNS tuberculosis is most common. In my study mortality is higher among 1-to-5-year age group with co morbid condition are SAM and septicemia.

TO DETERMINE THE ROLE OF CA125 IN THE DIAGNOSIS OF ACUTE APPENDICITIS

2020 ◽  
Vol 4 (7) ◽  
Author(s):  
Vinod Kumar ◽  
Bhupen Songra ◽  
Richa Jain ◽  
Deeksha Mehta
Keyword(s):  
Acute Appendicitis ◽  
Clinical Diagnosis ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Ca 125 ◽  
Care Hospital ◽  
Serum Samples ◽  
Roc Curve Analysis ◽  
Surgery Department

Background: the present study was under taken to determine the role of CA-125 in the diagnosis of acute appendicitis (AA), to prevent its complications and also in preventing negative appendicectomies in tertiary care hospital. Methods: The study was conducted at a tertiary care and research center between 01/03/2018 to 30/06/2019. Patients admitted to the surgery department with diagnosis of AA were considered for the study. After informed consent, a, standardized history was obtained as a case Performa. Serum samples from all the cases with clinical diagnosis of AA were obtained and stored. Only the cases with histopathologically approved AA were included in the study. Cases operated for clinical diagnosis of AA, but not histopathologically proven AA was not included in the study. CA125 levels in cases with definitive diagnosis of AA were measured. Results: In present study, ROC curve analysis revealed the sensitivity of 87.27 % and specificity of 90.91 % when the CA 125 cut-off value of > 16.8 was taken to diagnose acute appendicitis. AUC was 0.911 with a standard error of 0.0292. Conclusion: In this study we have observed that CA125 showed a positive correlation with acute appendicitis, that was statistically not significant (P>0.05). We didn’t evaluate the correlation with the disease severity. We consider that CA125 can be used as a marker in acute appendicitis cases although further research is still needed. Keywords: CA125, Acute Appendicitis, Surgery.

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