scholarly journals Caracterización bioquímica del síndrome nefrótico de una población pediátrica en Villahermosa, Tabasco. / Biochemical characterization of the nephrotic syndrome of a pediatric population in Villahermosa, Tabasco.

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Josefina Salomón-Cruz
Keyword(s):  
Nephrotic Syndrome ◽  
Retrospective Study ◽  
Biochemical Characterization ◽  
Clinical Laboratory ◽  
Pediatric Population ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
Palabras Clave ◽  
Para Determinar

ResumenEl síndrome nefrótico  se diagnóstica con base en cuatro criterios: proteinuria, hipoalbuminemia, edema e hipercolesterolemia. Generalmente se presenta entre los 2 y 10 años de edad. Este trabajo se realizó para determinar la caracterización bioquímica del síndrome nefrótico de la población infantil del Hospital del Niño de Villahermosa, Tabasco, México.Estudio descriptivo, transversal, retrospectivo realizado en el laboratorio clínico del Hospital del Niño Dr. Rodolfo Nieto Padrón de enero 2016 a diciembre 2017. Se obtuvo el rango y promedio de las pruebas bioquímicas de pacientes con síndrome nefrótico.Se analizaron 33 pacientes referidos con  diagnóstico de síndrome nefrótico,  encontrándose una media de albumina de 1.3g/dl, colesterol de 482.6 mg/dl y triglicéridos de 523.2 mg/d y una proteinuria media de 295.9 mg/dll. Palabras clave: Síndrome nefrótico,  Hipoalbuminemia, Hipercolesterolemia.   AbstractThe nephrotic syndrome is diagnosed based on four criteria: proteinuria, hypoalbuminemia, edema and hypercholesterolemia. It usually occurs between 2 and 10 years of age. This work was carried out to determine the biochemical characterization of the nephrotic syndrome of the infant population of the Hospital del Niño de Villahermosa, Tabasco, Mexico.Methodology:. Descriptive, cross-sectional, retrospective study conducted in the clinical laboratory of the Dr. Rodolfo Nieto Padrón Children's Hospital from January 2016 to December 2017. The range and average of the biochemical tests of patients with nephrotic syndrome was obtained.Results Thirty-three referred patients with a diagnosis of nephrotic syndrome were analyzed, finding a mean albumin of 1.3 g / dl, cholesterol of 482.6 mg / dl and triglycerides of 523.2 mg / d and an average proteinuria of 295.9 mg / dL. Key words: Nephrotic syndrome, Hypoalbuminemia, Hypercholesterolemia.

scholarly journals Genetic and Biochemical Characterization of the Chromosomal Class A β-Lactamases of Raoultella (formerly Klebsiella) planticola and Raoultella ornithinolytica

2004 ◽  
Vol 48 (1) ◽  
pp. 305-312 ◽  
Author(s):  
Estelle Walckenaer ◽  
Laurent Poirel ◽  
Véronique Leflon-Guibout ◽  
Patrice Nordmann ◽  
Marie-Hélène Nicolas-Chanoine
Keyword(s):  
Biochemical Characterization ◽  
Clinical Laboratory ◽  
Hydrolytic Activity ◽  
Amino Acid Identity ◽  
Resistance Pattern ◽  
Strain Atcc ◽  
Biochemical Tests ◽  
Class A ◽  
Amplification Method

ABSTRACT Enterobacterial strains of Raoultella spp. display a penicillinase-related β-lactam resistance pattern suggesting the presence of a chromosomal bla gene. From whole-cell DNA of Raoultella planticola strain ATCC 33531T and Raoultella ornithinolytica strain ATCC 31898T, bla genes were cloned and expressed into Escherichia coli. Each gene encoded an Ambler class A β-lactamase, named PLA-1 and ORN-1 for R. planticola and R. ornithinolytica, respectively. These β-lactamases (291 amino acids), with the same pI value of 7.8, had a shared amino acid identity of 94%, 37 to 47% identity with the majority of the chromosome-encoded class A β-lactamases previously described for Enterobacteriaceae, and 66 to 69% identity with the two β-lactamases LEN-1 and SHV-1 from Klebsiella pneumoniae. However, the highest identity percentage (69 to 71%) was found with the plasmid-mediated β-lactamase TEM-1. PLA-1, which displayed very strong hydrolytic activity against penicillins, also displayed significant hydrolytic activity against cefepime and, to a lesser extent, against cefotaxime and aztreonam, but there was no hydrolytic activity against ceftazidime. Such a substrate profile suggests that the Raoultella β-lactamases PLA-1 and ORN-1 should be classified into the group 2be of the β-lactamase classification of K. Bush, G. A. Jacoby, and A. A. Medeiros (Antimicrob. Agents Chemother. 39:1211-1233, 1995). The highly homologous regions upstream of the bla PLA-1A and bla ORN-1A genes comprised a nucleotide sequence identical to the −35 region and another one very close to the −10 region of the bla LEN-1 gene. From now on, as the bla gene sequences of the most frequent Raoultella and Klebsiella species are available, the bla gene amplification method can be used to differentiate these species from each other, which the biochemical tests currently carried out in the clinical laboratory are unable to do.

scholarly journals Comparison of API and Minitek to Center for Disease Control methods for the biochemical characterization of anaerobes

1976 ◽  
Vol 4 (3) ◽  
pp. 227-231 ◽  
Author(s):  
S L Hansen ◽  
B J Stewart
Keyword(s):  
Disease Control ◽  
Biochemical Characterization ◽  
Clinical Laboratory ◽  
Ease Of Use ◽  
Gas Liquid Chromatography ◽  
Control Methods ◽  
Conventional Tests ◽  
Reference Strains ◽  
Laboratory Time

Two commercially available micromethod multitest systems (API, Analytab Products, Inc., Minitek-Bioquest) were compared with conventional tests suggested by the Center for Disease Control for the identification of anaerobes. Anaerobiosis for the microsystems was achieved using GasPak system (BBL), A total of 175 anaerobes, including 158 clinical isolates and 17 reference strains, were used. Gram morphology, gas-liquid chromatography data, and biochemical reactions from the Center for Disease Control and Virginia Polytechnic Institute anaerobic manuals were used to identify the organisms. The Minitek system included a new anaerobe inoculum broth and two new disks, dextrose without nitrate and nitrate reductase disks. The percentage of correlation of 12 biochemicals using Minitek and 11 biochemicals using the API were compared with the Center for Disease Control reactions. The percentage of correlation of both positive and negative reactions with the API anaerobic strip ranged from 70.8 to 99.4% and with the Minitek from 97.1 to 100%. The microsystems were also evaluated as to the ease of use, adaptabilty to a clinical laboratory, time, and cost.

scholarly journals Clinical, genetic and microbiological characterization of pediatric patients with Cystic Fibrosis in a Public Hospital in Ecuador

2020 ◽  
Author(s):  
Yazmina Lascano-Vaca ◽  
Esteban Ortiz-Prado ◽  
Lenin Gomez-Barreno ◽  
Katherine Simbaña-Rivera ◽  
Eduardo Vasconez ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Public Hospital ◽  
Pediatric Patients ◽  
Pediatric Population ◽  
Clinical Genetic ◽  
Cross Sectional ◽  
Genetic Feature ◽  
Bacteriological Profile ◽  
Sectional Analysis

Abstract Background: To carry out a complete clinical, pathological, genetic and microbiological characterization of pediatric patients with molecular confirmed cystic fibrosis (CF) attending the Carlos Andrade Marín Hospital (HCAM) within the study period. Methods: A cross-sectional analysis of the pediatric population with a confirmed diagnosis of CF disease who attended HCAM , one of the largest tertiary-level hospitals in Ecuador, between 2017 and 2018 was performed. All demographic, clinical and genetic variables were obtained from the electronic medical records (EMR) stored by the hospital. Results: 47 patients with CF were included in the study. Gender distribution was similar between male (48.9%, n = 23) and female patients (51.1%, n = 24). The Tiffeneau-Pinelli index (FEV 1 /FVC) changed significantly after nine months post-diagnosis (85.55 ± 13.26; p < 0.05). The most common pathogenic genetic variants were F508del, found in 52.78% of the cohort (n = 19); H609R, found in 36.11% (n = 13); g.204099A>C, found in 14.1% (n = 7), followed by G85E and the N1303K with 11.11% (n = 3) each. Conclusions: To our best knowledge, this is the first study exploring the clinical, genetic and bacteriological profile of CF’s patients in Ecuador. Within the cohort of patients, an important and unique genetic feature was characterized by the presence of the g.204099A> C and the c.206359C>A homozygous polymorphism as well as the presence of the H609R variant, a mutation only reported among Ecuadorians.

scholarly journals DIAGNÓSTICO DE ESTÁNDARES DE CALIDAD INTERNACIONALES EN LABORATORIOS DE DOCENCIA DE UNA UNIVERSIDAD ECUATORIANA

2021 ◽  
Vol 6 (5) ◽  
pp. 4
Author(s):  
Ronny Rodríguez Pincay ◽  
William Lino Villacreses ◽  
Nereida Valero Cedeño ◽  
Alfonso Jamil Cedillo Balcázar
Keyword(s):  
Clinical Laboratory ◽  
Cross Sectional Study ◽  
System Quality ◽  
Cross Sectional ◽  
Iso 15189 ◽  
Teaching Laboratory ◽  
Palabras Clave ◽  
A Site ◽  
And Control ◽  
Teaching Site

Introducción: El Laboratorio de Docencia es un sitio donde el estudiante puede acceder a ella en su proceso de aprendizaje, al igual que el Laboratorio Clínico, están ligados a normas que se deben de cumplir para poder funcionar como sitio de enseñanza, un sistema de gestión de calidad son actividades coordinas para dirigir y controlar una organización con respecto a la calidad, están definidas por la Organización Internacional de Normalización (ISO), los indicadores estándar son aplicados de manera internacional y adaptables a cualquier tipo de laboratorio. Objetivo: diagnosticar los estándares de calidad internacionales en laboratorios de docencia una universidad ecuatoriana. Métodos: diseño de estudio descriptivo y analítico de corte transversal, se utilizaron fuentes bibliográficas basadas en fuentes primarias, secundarias y terciarias, el universo total fue de 4 laboratorios de docencia; para la recolección de información de utilizaron dos instrumentos validados por la OPS/HSP/HSE y el MSP del Ecuador. Resultados: Se encontró que, del total de indicadores de la primera encuesta, el 52,6% equivalente a 20 parámetros se cumplen, mientras que el 47,4% (n:18) no lo hace; en la segunda encuesta el 53,3% (n:16) cumple con lo requerido, mientras que el 46,7% (n:14) no lo hace. Conclusiones: los indicadores estándares son importantes a la hora de la pedagogía en los laboratorios de docencia de la Carrera de Laboratorio Clínico de la UNESUM. Palabras clave: Gestión de la calidad, Normas ISO 15189, Laboratorio de Docencia, Calidad   ABSTRACT Introduction: The Teaching Laboratory is a site where the student can access it in their learning process, like the Clinical Laboratory, they are linked to standards that must be met in order to function as a teaching site, a management system Quality are coordinated activities to direct and control an organization with respect to quality, they are defined by the International Organization for Standardization (ISO), the standard indicators are applied internationally and adaptable to any type of laboratory. Objective: diagnose international quality standards in teaching laboratories an Ecuadorian university. Methods: descriptive and analytical cross-sectional study design, bibliographic sources based on primary, secondary and tertiary sources were used, the total universe was 4 teaching laboratories; Two instruments validated by PAHO / HSP / HSE and the MSP of Ecuador were used to collect information. Results: It was found that, of the total of indicators from the first survey, 52.6% equivalent to 20 parameters are met, while 47.4% (n:18) do not; in the second survey, 53.3% (n: 16) met the requirements, while 46.7% (n:14) did not. Conclusions: the standard indicators are important when it comes to pedagogy in the teaching laboratories of the Clinical Laboratory Career of UNESUM. Keywords: Quality management, ISO 15189 Standards, Teaching Laboratory, Quality

scholarly journals Conductas, actitudes y prácticas de la madre o cuidador en el manejo de la enfermedad diarreica aguda en menores de cinco años

2015 ◽  
Vol 12 (2) ◽  
pp. 39
Author(s):  
María Del Pilar Gallardo-Lizarazo
Keyword(s):  
Acute Diarrhea ◽  
Statistical Validation ◽  
Cross Sectional ◽  
Children Under Five ◽  
Under Five ◽  
Attitudes And Practices ◽  
Proportional Allocation ◽  
Family Tradition ◽  
Palabras Clave ◽  
Para Determinar

Objetivo: determinar las creencias de la madre y del cuidador sobre el manejo de la enfermedad diarreica aguda en menores de 5 años de edad en la ciudad de Bucaramanga en el año 2015. Materiales y Métodos: la investigación se enmarca en el paradigma cuantitativo mediante el desarrollo de estudio descriptivo de corte transversal; se encuestaron 200 madres o cuidadores de menores de cinco años en la ciudad de Bucaramanga, seleccionados mediante muestreo aleatorio estratificado con afijación proporcional, confiabilidad 95% y margen de error de 7%. Para la recolección de la información se empleó una encuesta elaborada por la autora con validación estadística y por expertos. Resultados: se obtiene información sobre conocimientos y tradiciones acerca del manejo de la diarrea y la identificación de los signos de deshidratación por parte de los cuidadores, que son analizados en comparación con sus características sociales y culturales. Conclusiones: en Bucaramanga, madres y cuidadores de menores de cinco años, continúan la tradición familiar en lo referente a identificación de signos de deshidratación y costumbres en el manejo de la diarrea; la mayoría acude a consulta médica para su tratamiento; sin embargo, se encuentra un alto porcentaje que también recurre al tegua–sobandero. Para el manejo de la diarrea, las madres o cuidadores suministran caldos a los menores, aumentan la cantidad de líquidos, les administran sales de rehidratación oral y jugo de guayaba. Se encuentran diferencias acerca de las costumbres y tradiciones entre estratos socioeconómicos y niveles de escolaridad. PALABRAS CLAVE: costumbres, cuidadores, diarrea infantil . Behaviors, attitudes and practices of the mother or caregivers in the management of acute diarrhea in children under five years  ABSTRACT Goal: to determine the beliefs of mothers and caregivers about the management of acute diarrhea in children under 5 years old in Bucaramanga city in the year 2015. Materials and Methods: the research is part of the quantitative paradigm by development of cross-sectional descriptive study; 200 mothers or caregivers of children under five were surveyed in Bucaramanga city,they were selected through stratified random sampling with proportional allocation, reliability and 95% margin of error of 7%. For data collection, a survey was conducted by the author with statistical validation and experts. Results: information on knowledge and traditions about the management of diarrhea and identify signs of dehydration by caregivers, which are analyzed in comparison with their social and cultural characteristics were obtained. Conclusions: in Bucaramanga city , mothers and caregivers of children under five years, continue the family tradition when it comes to checking for signs of dehydration and the old fashioned method in the management of diarrhea; most of them  go to see their doctor for treatment; however, there is a high percentage that also relies `Tegua-sobandero`. For the management of diarrhea, mothers or caregivers provide wines to children, increase the amount of fluids administered them ORS and guava juice.There were some differences concerning to the customs and traditions between socioeconomic and educational levels.KEYWORDS: customs, caregivers, childhood diarrhea.Comportamentos, atitudes e práticas da mãe ou responsável na gestão da diarreia aguda em crianças menores de cinco anos ABSTRACT  Objetivo: para determinar as crenças de mães e cuidadores sobre a gestão da diarreia aguda em crianças menores de 5 anos na cidade de Bucaramanga em 2015. Materiais e Métodos: a pesquisa é parte do paradigma quantitativo por desenvolvimento de estudo descritivo transversal; 200 mães ou cuidadores de crianças menores de cinco anos foram pesquisados na cidade de Bucaramanga, selecionadas por amostragem aleatória estratificada com alocação proporcional, confiabilidade e margem de 95% de erro de 7%. Para pesquisa de coleta de dados realizada pelo autor com validação estatística e os peritos que ele estava acostumado. Resultados: informações sobre o conhecimento e tradições sobre a gestão de diarreia e identificar sinais de desidratação por cuidadores, que são analisadas em comparação com as suas características sociais e culturais é obtido. Conclusões: em Bucaramanga, mães e cuidadores de crianças menores de cinco anos, continuar a tradição da família quando se trata de verificar se há sinais de desidratação e alfândegas na gestão da diarreia; mais ir ver o seu médico para tratamento; no entanto, há uma alta porcentagem que também se baseia Tegua-sobandero. Para a gestão de diarreia, mães ou cuidadores fornecem vinhos a menores, aumentar a quantidade de fluidos administrados los ORS e suco de goiaba. diferenças sobre os costumes e tradições entre os níveis socioeconômicos e educacionais são.PALAVRAS-CHAVE: costumes, cuidadores, diarreia infantil. 

scholarly journals Isolation and Characterization of Commercial Probiotics

2020 ◽  
Vol 11 (1) ◽  
pp. 818-825
Author(s):  
Kolli Guna Ranjan ◽  
Girija Sankar G ◽  
Satyanarayana Raju D V V
Keyword(s):  
Biochemical Characterization ◽  
Specific Treatment ◽  
Growth Patterns ◽  
Genetic Identification ◽  
Molecular Technique ◽  
Evolutionary Analysis ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
Commercial Probiotics

Commercial probiotics have bacteria which offer scope for specific treatment as a probiotic against diarrhoea, boosting immune response and relieving stress. Research in probiotics and aquaculture has been done to promote sustainable aquaculture. Studies on microbial ecology in aquaculture and their benefits on human systems are to be assessed. Hence, the present study was aimed at the isolation and characterization of these bacteria obtained from various commercial probiotics. The commercial probiotic samples were collected for isolation using MRS agar. The colonies were selected on the basis of colonial morphology.  Isolates were put through cell morphology, physiology and different biochemical tests. Probiotic sample confirmation was done using 16srRNA molecular technique. MEGA7 was used to conduct phylogenetic evolutionary analysis and tabulate a distance matrix. In results, the isolates manifested non-identical growth patterns at dissimilar conc. of NaCl (2.0,4.0 and 6.5) oxygen and at different temperatures (150C, 300C and 450C). On the basis of sugar utilization, physiological testing, biochemical characterization and genetic identification tests, all isolates were established to different species of Lactobacillus rhamnosus, Pediococcus acidilactici and Lactobacillus Plantarum. A systematic protocol was done to identify, characterize probiotic samples and identify them by genetic analysis. Probiotic use is carefully assessed by regulations

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