scholarly journals MORPHOMETRIC STUDY OF THE SMALL INTESTINE OF RAT-ADOLESCENTS IN CHRONIC INTOXICATION WITH LEAD ACETATE

2018 ◽  
Vol 67 (3) ◽  
pp. 69-71
Author(s):  
P.A. Elyasin ◽  
◽  
S.V. Zalavina ◽  
A.N. Mashak ◽  
A.P. Nadeev ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lead Acetate ◽  
Morphometric Study ◽  
Chronic Intoxication

scholarly journals MORPHOMETRIC STUDY OF ISOLATED DOGIEL TYPE II CELLS OF THE INTERMUSCULAR PLEXUS

2021 ◽  
Vol 11 (3) ◽  
pp. 17-19
Author(s):  
Ramilya Babaeva
Keyword(s):  
Small Intestine ◽  
3D Model ◽  
Longitudinal Direction ◽  
3D Models ◽  
Morphometric Study ◽  
Type Ii Cells ◽  
Impregnation Method ◽  
Type Ii ◽  
Type Ii Cell ◽  
Dogiel Type Ii

Aim of the study was to calculate the morphometric parameters of isolated Dogiel type II cells from the intermuscular plexus of the small intestine. Materials and methods: 3D models of the oval-shaped Dogiel type II cells from the intermuscular plexus of a rat (n = 1) were constructed and studied. Neurocytes were identified by the impregnation method on the frontal and horizontal sections of the wall of the small intestine. Results: The results showed that the total number of the nodes in the virtual model was 36534, and the mesh elements — 156595. The resulting 3D model of the cell and nucleus was reduced 900 times to obtain a threedimensional cell and nucleus with absolute dimensions, with a ratio of 1:1 to their true size. The volume of Dogiel type II cell was 2785.11 μm³, the volume of the nucleus was 647.7 μm³ and the volume of its perikaryon was 2785.11 μm³. Conclusion: Dogiel type II cells from the intermuscular plexus of the rat small intestine has an ovoid shaped threedimensional structure. These cells are flattened in transverse direction and elongated in longitudinal direction.

scholarly journals Anatomical and Morphometric Study of Small and Large Intestine of Bali Cattle

2020 ◽  
Vol 3 (2) ◽  
pp. 86
Author(s):  
Luh Gde Surya Heryani ◽  
Ni Nyoman Werdi Susari ◽  
Luh Putu Syamadina Pramesya Nareswari
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Digestive System ◽  
Average Length ◽  
Morphometric Study ◽  
Preclinical Research ◽  
Colon And Rectum ◽  
Bali Cattle ◽  
The Right

Information on the anatomical and morphometric structure of the digestive system of Bali cattle will provide a clear and precise picture if abnormalities occur in the organs in the digestive system. This study aims to determine the anatomical and morphometric structures of the small intestine and large intestine of Bali cattle. Samples were taken from 14 Bali cattle and based on gross pathology examination declared healthy. The results showed that the average length of the small intestine including the duodenum, jejunum and ileum are 473.07 cm, 529.64 cm, and 363.64 cm; and successive widths are 5.50 cm, 6.00 cm, and 6.11 cm. While the average length of the large intestine which includes the cecum, colon and rectum are 36.78 cm, 371.21, 50.00 cm; while the width are 9.65 cm, 11.47 cm, and 8.85 cm. Jejunum has the longest size in the small intestine, while in the large intestine the longest size is the colon. The right information and data about the digestive system are very important and useful to support further clinical and preclinical research, and the results of this study can be used as a reference in determining the characterization of Bali cattle.

Morphometric study of the guinea pig small intestine during development

2004 ◽  
Vol 63 (4) ◽  
pp. 206-214 ◽  
Author(s):  
Montserrat Mitjans ◽  
Ruth Ferrer
Keyword(s):  
Small Intestine ◽  
Guinea Pig ◽  
Morphometric Study

Morphometric study of the layers of the canine small intestine at five sampling sites

2012 ◽  
Vol 192 (3) ◽  
pp. 498-502 ◽  
Author(s):  
R. Sarriá ◽  
R. Latorre ◽  
M. Henroteaux ◽  
N. Henroteaux ◽  
F. Soria ◽  
...  
Keyword(s):  
Small Intestine ◽  
Morphometric Study

Plasmalemma localisation of inorganic phosphate in B cells pancreas

1978 ◽  
Vol 36 (2) ◽  
pp. 528-529
Author(s):  
F. B. P. Wooding ◽  
K. Pedley ◽  
N. Freinkel ◽  
R. M. C. Dawson
Keyword(s):  
Electron Microscope ◽  
B Cells ◽  
B Cell ◽  
Pancreatic Islets ◽  
High Glucose ◽  
Lead Acetate ◽  
Inorganic Phosphate ◽  
Slight Modification ◽  
Uranyl Acetate ◽  
Lead Phosphate

Freinkel et al (1974) demonstrated that isolated perifused rat pancreatic islets reproduceably release up to 50% of their total inorganic phosphate when the concentration of glucose in the perifusion medium is raised.Using a slight modification of the Libanati and Tandler (1969) method for localising inorganic phosphate by fixation-precipitation with glutaraldehyde-lead acetate we can demonstrate there is a significant deposition of lead phosphate (identified by energy dispersive electron microscope microanalysis) at or on the plasmalemma of the B cell of the islets (Fig 1, 3). Islets after incubation in high glucose show very little precipitate at this or any other site (Fig 2). At higher magnification the precipitate seems to be intracellular (Fig 4) but since any use of osmium or uranyl acetate to increase membrane contrast removes the precipitate of lead phosphate it has not been possible to verify this as yet.

A complex network of “snake-like tubules” revealed by the NADPase cytochemical reaction in the acinar cell of pancreas

1984 ◽  
Vol 42 ◽  
pp. 666-667
Author(s):  
A.R. Beaudoin ◽  
G. Grondin ◽  
A. Lord ◽  
M. Pelletier
Keyword(s):  
Acinar Cell ◽  
Lead Acetate ◽  
Ultrastructural Localization ◽  
Sodium Acetate Buffer ◽  
Zymogen Granules ◽  
Sprague Dawley ◽  
Dense Bodies ◽  
Sprague Dawley Rats ◽  
Cytochemical Reaction ◽  
Cellular Organelles

We have recently described the ultrastructural localization of NADPase activity in the exocrine pancreas of rat. The enzyme was found in the intermediate saccules of the Golgi apparatus, in dense bodies and lysosomes but was absent from zymogen granules. A very intense reaction was noticed in a peculiar structure which was termed “Snake-Like Tubule” (SLT). The purposes of the present study were firstly to delineate SLT distribution in the acinar cell and secondly to define any possible relationship or association with other cellular organelles.NADPase cytochemical reaction was performed on the pancreas of adult Sprague Dawley rats. Small lobules were excised and fixed for 50 min, at 4°C, in 2% glutaraldehyde buffered with 0.1M cacodylate at pH 7.2. Lobules were rinsed several times with the same buffer containing 570 sucrose and cut with a Mcllwayn tissue chopper. Sections were washed several times with buffer and incubated for 2 hr at 37°C in the following medium: 4mM NADPH; 40mM sodium acetate buffer, pH 5.0; 4mM lead acetate and 5% sucrose.

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

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