Status and challenges for the molecular characterization of Fannia (Insecta, Diptera, Fanniidae) in Brazil

Revista dos Trabalhos de Iniciação Científica da UNICAMP ◽

10.20396/revpibic262018186 ◽

2018 ◽

Author(s):

Bárbara Maria Cerqueira Maia ◽

Patricia Jacqueline Thyssen ◽

Taís Madeira Ott

Keyword(s):

Molecular Characterization ◽

Nucleotide Sequences ◽

Time Of Death ◽

Species Determination ◽

Barcode Dna ◽

Characterization Studies

Both the advancement of technology associated with molecular characterization studies of species have contributed to the increased use of the Barcode DNA method for identifying individuals. For some areas, such as forensics, species determination is essential for information regarding the time of death. In this study, Fannia nucleotide sequences available in public databases were quantified and used to evaluate the scenario and the challenge to establish molecular research with fanids in Brazil.

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Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

Ciência Rural ◽

10.1590/0103-8478cr20141810 ◽

2015 ◽

Vol 45 (12) ◽

pp. 2197-2200 ◽

Cited By ~ 2

Author(s):

Thor Vinícius Martins Fajardo ◽

Monique Bezerra Nascimento ◽

Marcelo Eiras ◽

Osmar Nickel ◽

Gilvan Pio-Ribeiro

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Molecular Analysis ◽

Nucleotide Sequences ◽

Amino Acid Sequences ◽

Causal Agent ◽

Ringspot Virus ◽

Prunus Necrotic Ringspot Virus ◽

First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

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MOLECULAR CHARACTERIZATION OF TWO NOVEL RAT MINUTE VIRUSES IN TAIWAN

Taiwan Veterinary Journal ◽

10.1142/s1682648520500109 ◽

2020 ◽

Vol 46 (02n03) ◽

pp. 101-110

Author(s):

Yi-Chun Liao ◽

Yung-Hui Chang ◽

Ming-Hseng Wang ◽

Ber-Hsiang Fang ◽

Cho-Hua Wan

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Nucleotide Sequences ◽

Amino Acid Sequences ◽

Phylogenetic Data ◽

Laboratory Rodent ◽

Minute Virus ◽

The Common ◽

Kilham Rat Virus

Rodent parvovirus infection is one of the common viral problems in laboratory rodent colonies. In this study, two new parvoviruses were identified in naturally-infected rats from two different research colonies in Taiwan. The genomic nucleotide sequences and the predicted amino acid sequences of proteins for these two viruses were compared to the previously characterized rodent parvoviruses. The two newly identified viruses were most closely related to each other, also closely related to two variants of rat minute virus (RMV; RMV-1 and RMV-2), and distinct from but closely related to Kilham rat virus and H-1 virus. These two viruses were significantly different from variants of rat parvovirus (RPV; RPV-1 and RPV-NTU1). Phylogenetic data also supported that these two new viruses are variants of the RMV species. These two newly identified viruses were designated RMV type National Taiwan University 1 (RMV-NTU1) and RMV type National Taiwan University 2 (RMV-NTU2). RMV-NTUs are the first molecularly characterized RMV variants identified in Asia.

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Molecular characterization of nucleotide sequences encoding the extracellular glycoprotein elicitor from Phytophthora megasperma

MGG Molecular & General Genetics ◽

10.1007/bf00290132 ◽

1995 ◽

Vol 246 (1) ◽

pp. 45-55 ◽

Cited By ~ 43

Author(s):

Wendy Sacks ◽

Thorsten Nürnberger ◽

Klaus Hahlbrock ◽

Dierk Scheel

Keyword(s):

Molecular Characterization ◽

Nucleotide Sequences ◽

Phytophthora Megasperma ◽

Extracellular Glycoprotein

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Molecular characterization of medically important viruses of the genus Orthobunyavirus

Journal of General Virology ◽

10.1099/vir.0.2008/002253-0 ◽

2008 ◽

Vol 89 (10) ◽

pp. 2580-2585 ◽

Cited By ~ 28

Author(s):

Amy J. Lambert ◽

Robert S. Lanciotti

Keyword(s):

Molecular Characterization ◽

Sequence Similarity ◽

Phylogenetic Analyses ◽

Nucleotide Sequences ◽

Full Length ◽

Human Pathogens ◽

Rna Sequences ◽

Virus Family ◽

Close Sequence

We have characterized the full-length S segment RNA sequences of five human pathogens of the virus family Bunyaviridae, genus Orthobunyavirus. S segment sequences of Fort Sherman, Shokwe and Xingu viruses of the Bunyamwera serogroup, as well as those of Bwamba and Pongola viruses of the Bwamba serogroup, are described. S segment sequences of Bwamba and Pongola viruses represent the first nucleotide sequences characterized for viruses of the Bwamba serogroup. The described molecular and phylogenetic analyses of these and other selected viruses of the genus Orthobunyavirus reveal that a close sequence similarity is shared between the African Bwamba and the predominantly North American and European California serogroups of the genus Orthobunyavirus.

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Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt

Advances in Virology ◽

10.1155/2014/797151 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 7

Author(s):

Mohammed AboElkhair ◽

Alaa G. Abd El-Razak ◽

Abd Elnaby Y. Metwally

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Nucleotide Sequences ◽

Amino Acid Sequences ◽

Chicken Anemia Virus ◽

Coding Region ◽

Commercial Chicken ◽

Amino Acids Profile ◽

Anemia Virus

Introduction. Although many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published. In the present study, four nucleotide sequences of detected CAV were genetically characterized.Methods. These nucleotide sequences were obtained from commercial chicken flocks in two different locations of Egypt during 2010. The target region for sequencing was 675 bp nucleotide of partial coding region of VP1 protein. The nucleotide and deduced amino acid sequences of the detected CAV were aligned and compared to worldwide CAV isolates including commonly used vaccine strains. Phylogenetic analysis of these sequences was also carried out.Results. Our results showed that all the Egyptian CAV sequences were grouped in one group with viruses from diverse geographic regions. This group is characterized by amino acids profile75I,97L,139Q, and144Q in VP1. The phylogenetic and amino acid analyses of deduced amino acid indicated that the detected CAV sequences differ from CAV vaccine strains.Conclusion. This is the first report that describes molecular characterization of circulating CAV in Egypt. The study showed that the detected CAV, in Egypt are field viruses and unrelated to vaccine strains.

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