The Effects of High-fat Diet on Histological Changes of Kidneys in Rats

Introduction Hypercholesterolemia contributes as a risk factor for endothelial dysfunction in the kidneys and worsens the condition of patients with previous kidney damage. This study aimed to examine the effect of hypercholesterol diet in kidney structure.Methods: This was an experimental study, a case control using eight-week-old Rattus novergicus, divided into control and treatment group. High-fat diet (HFD) was administrated orally to treatment group for 14 days. All data statistically analyzed with two-paired ANOVA and post hoc for comparison.Results: Histological analysis using hematoxylin-eosin staining showed degeneration in nephrons, including glomerulosclerosis, segmental necrosis and tubular defects. The mononuclear cell infiltration was Increased in HFD group significantly (P<0.05).Conclusion: We concluded that a high fat diet induced obesity and may lead to renal deformities as a result of histopathological changes such as dilatation, tubular defects, inflammation and connective tissue enlargement of the kidney.

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Histopathology from liver of tuvira ( Gymnotus spp.) parasitized by larvae of nematodes

Ciência Rural ◽

10.1590/0103-8478cr20150881 ◽

2016 ◽

Vol 46 (7) ◽

pp. 1233-1239 ◽

Cited By ~ 6

Author(s):

Arlene Sobrinho Ventura ◽

Márcia Mayumi Ishikawa ◽

Andrea Maria de Araújo Gabriel ◽

Helcy Lylian Nogueira Silbiger ◽

Fabiana Cavichiolo ◽

...

Keyword(s):

Histological Analysis ◽

Parasitic Infection ◽

Fish Farming ◽

Health Condition ◽

Histopathological Changes ◽

Hepatic Parenchyma ◽

Histological Changes ◽

Balance Relation ◽

Liver Changes ◽

Hematoxylin Eosin

ABSTRACT: The aim of this study was to evaluate the histological changes in the liver of thirty-five Gymnotusspp. parasitized by endohelminths collected between April 2012 to October 2013 in commercial bait fish farming of Pantanal basin. Histological cuts of 7µm were stained with hematoxylin-eosin for parasites research and liver changes and have also been submitted to the Perls histochemical method for evaluation of hemosiderosis (Fe+++) based on the incidence degree and severity of change (Grade I, II and III) and tests for the presence of central melanomacrophages. Parasites identified were: Brevimulticaecumsp. with a prevalence of 22,9%, Eustrongylidessp 17,1%, Contracaecumtype I 68,7%, Contracaecumtype II 5,7%, Contracaecumtype III 5,7% and larvae of Anisakidae11,4%. Histological analysis showed intense disorganization of hepatic parenchyma with degenerate hepatocytes due to high parasitic infection, changes that can be deleterious and compromise the organism functioning, being harmful to the health of evaluated animals. Also evidencing normal tissue interleaved with different stages of Fe+++ deposit in grades II and III, injuring or destroing the cell. Histopathological changes in the tuvira's liver suggested a chronic response and the development of a balance relation between tuvira and parasitism by endohelminth identified in this study. There are also a testimony to the health condition of commercial bait fish farming on current ecosystem conditions.

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