ANALYSIS OF THE EXPRESSION OF FAS/CD95 AND HSP70 IN LOW AND HIGH GRADE UROTHELIAL CELL CARCINOMA OF THE BLADDER

Urothelial Cell Carcinoma (UCC), also called transitional cell carcinoma of the bladder, is the most common malignancy in urinary tract. The prognosis of this disease is highly dependent on the histological grading at diagnosis. Fas/CD95 has a role in apoptotic process, whereas HSP70 has an antiapoptotic role. This study aimed to analyze the expression of Fas/CD95 and HSP70 in low grade and high grade urothelial cell carcinoma in the bladder. This was an analytic observational study with cross sectional approach. Population and sample were paraffin blocks of urothelial cell carcinoma in the Laboratory of Anatomic Pathology, Dr. Soetomo Hospital, Surabaya in the period of January 2011-December 2016. Each grading of urothelial cell carcinoma was randomly sampled. Immunohistochemystry with Fas/CD95 and HSP70 were performed. Expression of Fas/CD95 and HSP70 were assessed semiquantitatively. Expression of Fas/CD95 and HSP70 were analyzed using Mann-Whitney test and Spearman test. The results showed there was significant different in expression of Fas/CD95 and HSP70 in low and high grades in urothelial cell carcinoma. There was no significant correlation between the expression of Fas/CD95 and HSP70 in urothelial cell carcinoma. As a conclusion, the role of CD95 and HSP70 expression can be useful as marker for the diagnosis, especially in the determination of the grade of differentiation.

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Bacillus Calmette–Guérin (BCG) Immunotherapy for Recurrent Noninvasive Transitional Cell Carcinoma of the Bladder

50 Studies Every Urologist Should Know ◽

10.1093/med/9780190655341.003.0024 ◽

2021 ◽

pp. 135-140

Author(s):

Vikram M. Narayan

Keyword(s):

Transitional Cell Carcinoma ◽

Cell Carcinoma ◽

Carcinoma In Situ ◽

Transitional Cell ◽

High Grade ◽

Bacillus Calmette Guerin ◽

Urothelial Cell Carcinoma ◽

Carcinoma Of The Bladder ◽

Disease Specific

This study summarizes the landmark study assessing the value of maintenance intravesical Bacillus Calmette Guerin (BCG) to reduce the risk for recurrence or progression of high-grade superficial or carcinoma in situ (CIS) transitional cell carcinoma of the bladder. The trial randomized patients with completely resected noninvasive urothelial cell carcinoma at high risk of recurrence to BCG induction alone versus BCG induction plus maintenance therapy in the form of six weekly treatments. The study found that maintenance BCG reduces disease-specific recurrence and may also reduce the rate of disease progression.

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Higher Expression of miR-182 in Cytology Specimens of High-Grade Urothelial Cell Carcinoma: A Potential Diagnostic Marker

Acta Cytologica ◽

10.1159/000371507 ◽

2015 ◽

Vol 59 (1) ◽

pp. 109-112 ◽

Cited By ~ 3

Author(s):

Shuanzeng Wei ◽

Zhanyong Bing ◽

Yuan Yao ◽

Stephen R. Master ◽

Prabodh Gupta

Keyword(s):

Cell Carcinoma ◽

Noncoding Rna ◽

Urothelial Cell ◽

Low Grade ◽

High Grade ◽

Urothelial Cell Carcinoma ◽

Rna Molecules ◽

History Of ◽

Potential Alternative ◽

Normal Controls

Objective: MicroRNAs (miRs) are short noncoding RNA molecules that posttranscriptionally modulate protein expression. There are distinct miR alterations characterizing urothelial cell carcinoma (UCC) of the urinary bladder. Study Design: In this study, we investigate the possibility of using miR as a noninvasive marker in the screening of UCC. The total RNA was extracted from 75 cytology specimens including bladder or renal washings and voided urines. Cases comprise UCC (21 high grade and 6 low grade), 25 normal controls and 23 cases with a history of UCC but negative at the time of testing (negative with a positive history). The expressions of miR-96, miR-182, miR-183, miR-200c, miR-21, miR-141 and miR-30b were determined using quantitative TaqMan real-time PCR. Results and Conclusion: This study shows that the level of miR-182 is higher in cytology specimens from high-grade UCC patients as compared to normal controls. Measuring miR-182 may provide a potential alternative or adjunct approach for screening high-grade UCC.

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