Aggregatibacter actinomycetemcomitans sensitivity towards chlorophyll of Moringa leaf after activated by diode laser

Background: Regular brushing teeth with scaling and root planning (SRP) cannot effectively decrease the periopatogen bacterial colonies. Even with the addition of antibiotics to support SRP, such as tetracycline given with low doses and for a long time may cause bacteria to become resistant and the effectiveness to eliminate colonies of bacteria being reduced. Photodynamic is a treatment modality that does not cause resistance and potentially to eliminate the growth of bacterial colonies. Moringa oleifera is a plant that can be easily found in Indonesia, by extracting chlorophyll of Moringa oleifera leaves, it can be used as a photosensitizer agent to increase the absorption of light on photodynamic method. Purpose: This study aimed to determine the potential photodynamic inactivation therapy to inactivate (eliminate) periopatogen bacterium. Method: This study used Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Laser diode 660nm as a light source with 8mm optical fiber to guide the beam, also used 20% extract chlorophyll of Moringa oleifera leaf as photosensitizer. Four diode lasers energy density exposures (2,5J.cm-2, 5J.cm-2, 7,5J.cm-2, and 10J.cm-2) are used from both at the in vitro photodynamic inactivation test. Result: The highest percentage of deaths occurred in the group treated with addition of photosensitizer and exposed by 660 nm diode laser with 10J.cm-2 energy density, which is 83.01%, compared to the results obtained in the group without addition of the photosensitizer. Conclusion: Chlorophyll of Moringa oleifera leaf after activated by diode laser effectively eliminates A. actinomycetemcomitans.

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Effect of the in vitro gastrointestinal digestion on free-phenolic compounds and mono/oligosaccharides from Moringa oleifera leaves: Bioaccessibility, intestinal permeability and antioxidant capacity

Food Research International ◽

10.1016/j.foodres.2018.11.017 ◽

2019 ◽

Vol 120 ◽

pp. 631-642 ◽

Cited By ~ 8

Author(s):

L.H. Caicedo-Lopez ◽

I. Luzardo-Ocampo ◽

M.L. Cuellar-Nuñez ◽

R. Campos-Vega ◽

S. Mendoza ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Intestinal Permeability ◽

Moringa Oleifera ◽

Gastrointestinal Digestion ◽

In Vitro Gastrointestinal Digestion ◽

Moringa Oleifera Leaves

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Increasing the In Vitro Enzymatic Activity of Cellulase and Amylase from Beef Cattle Rumen Fluid Supplemented with Moringa oleifera Leaves and Sulfur

Pakistan Journal of Nutrition ◽

10.3923/pjn.2017.571.576 ◽

2017 ◽

Vol 16 (8) ◽

pp. 571-576

Author(s):

F.M. Suhartati ◽

W. Suryaprata

Keyword(s):

Beef Cattle ◽

Enzymatic Activity ◽

Moringa Oleifera ◽

Rumen Fluid ◽

Moringa Oleifera Leaves

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EVALUATION OF IN VITRO PROTEIN DIGESTIBILITY OF Moringa oleifera LEAVES WITH VARIOUS DOMESTIC COOKING

Carpathian Journal of Food Science and Technology ◽

10.34302/crpjfst/2021.13.1.18 ◽

2021 ◽

pp. 214-221

Keyword(s):

Moringa Oleifera ◽

Protein Digestibility ◽

In Vitro Protein Digestibility ◽

Moringa Oleifera Leaves ◽

Vitro Protein

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The efficacy of photodynamic inactivation with laser diode on Staphylococcus aureus biofilm with various ages of biofilm

Infectious Disease Reports ◽

10.4081/idr.2020.8736 ◽

2020 ◽

Author(s):

Suryani Dyah Astuti ◽

Hafidiana ◽

Riries Rulaningtyas ◽

Abdurachman ◽

Alfian P Putra ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Energy Density ◽

Diode Laser ◽

Laser Diode ◽

Elisa Test ◽

Control Group ◽

Photodynamic Inactivation ◽

Bacterial Cell Death ◽

Irradiation Energy ◽

Blue Diode Laser

Biofilms are able to casue microorganisms to be 80% more resistant to antibiotics. The extracelullar polymeric substance (EPS) in biofilm functions to protect bacteria, making it difficult for antibiotics to penetrate the biofilm layer. This study aims to determine the effective- ness of photodynamic inactivation with blue diode laser to reduce Staphylococcus aureus biofilm at various ages of biofilms. The light source is a 403 nm blue diode laser with an energy power of about 27.65±0.01 mW. The study was designed with two groups: Group C was the untreated control group with variations in age of biofilms (0; 6; 11; 17; 24; 32; 40 and 48) hours; Group T was a laser treatment group with variations in age of biofilm and energy density (4.23; 8.46; 12.70; 16.93 and 21.16) J/cm2. Biofilm reduction measurement method using ELISA test was performed to calculate OD595 value. The statistical analysis results of variance showed that there was an influence of biofilm age and irradiation energy density of laser on biofilm reduction. Optical density analysis showed the most optimum biofilm reduction happened when biofilm age is perfectly constructed (about 17 hours) and with 91% reduction. The longer biofilm age lived among those biofilms, the greater the reduction. The results of the Scanning Microscope Electron and fluorescent microscope measurement showed destruction site of the EPS biofilm and bacterial cell death. So, the activated photodynamic with 403 nm laser diode is effective to reduce the Staphylococcus aureus biofilm in the maturation phase.

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Optimization of Phenolic Compound Extraction from Chinese Moringa oleifera Leaves and Antioxidant Activities

Journal of Food Quality ◽

10.1155/2019/5346279 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Beibei Zhao ◽

Jiawen Deng ◽

Hua Li ◽

Yaqiang He ◽

Tao Lan ◽

...

Keyword(s):

Antioxidant Activity ◽

Moringa Oleifera ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Ultrasonic Extraction ◽

Extraction Temperature ◽

Moringa Oleifera Leaves

Rich in phenolic compounds, Moringa oleifera leaf extract (ME) exhibits significant antioxidant activity both in vitro and in vivo. ME has already been widely used in fields of medicine, functional food, and cosmetics. Ultrasonic extraction (UE) method has been improved to be one of the most effective ways to extract phenols from M. oleifera leaves. The purpose of this study was to optimize ultrasonic extraction of phenols by response surface methodology (RSM). Four parameters were discussed, such as ethanol concentration, solvent-sample ratio, extraction temperature, and extraction time. Also, purification methods of the crude ME by organic solvent extraction and column chromatography were examined. Antioxidant activities of ME and each fraction were evaluated by DPPH, ABTS, and hydroxy radical-scavenging activities and reducing power. The phenol content of the purified ME reached up to 962.6 mg RE/g, extremely higher than the crude extract 107.22 ± 1.93 mg RE/g. The antioxidant activity of the purified ME was also significantly improved. Furthermore, phenols were identified by using the HPLC-MS method, and the results showed that there were 6 phenolic acids and derivatives and 7 flavonoids in ME. Quercetin-3-O-β-D-glucoside isolated from ME showed excellent DPPH and ABTS radical-scavenging abilities, which were comparable to VC.

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