scholarly journals Comparison of miRNA cargo in human adipose-tissue vs. amniotic-membrane derived mesenchymal stromal cells extracellular vesicles for osteoarthritis treatment

2021 ◽  
Author(s):  
Enrico Ragni ◽  
Carlotta Perucca Orfei ◽  
Andrea Papait ◽  
Laura de Girolamo
Keyword(s):  
Adipose Tissue ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Amniotic Membrane ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Osteoarthritis Treatment

scholarly journals Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis

2021 ◽  
Author(s):  
Cansu Gorgun ◽  
Maria Elisabetta Federica Palamà ◽  
Daniele Reverberi ◽  
Maria Cristina Gagliani ◽  
Katia Cortese ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Endothelial Proliferation

scholarly journals Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile

2015 ◽  
Vol 24 (23) ◽  
pp. 2822-2840 ◽  
Author(s):  
Lindolfo da Silva Meirelles ◽  
Tathiane Maistro Malta ◽  
Virgínia Mara de Deus Wagatsuma ◽  
Patrícia Viana Bonini Palma ◽  
Amélia Goes Araújo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Mesenchymal Stromal Cells ◽  
Expression Profile ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Similar Gene Expression Profile ◽  
Similar Gene

scholarly journals Periostin expression and characters of human adipose tissue-derived mesenchymal stromal cells were aberrantly affected by in vitro cultivation

2019 ◽  
Vol 6 ◽  
pp. 33-33 ◽  
Author(s):  
Heba M. Saad Eldien ◽  
Hekmat Osman Abdel-Aziz ◽  
Douaa Sayed ◽  
Wafaa Mubarak ◽  
Hemmat H. G. Hareedy ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
In Vitro Cultivation

scholarly journals A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Valentina Coccè ◽  
Anna Brini ◽  
Aldo Bruno Giannì ◽  
Valeria Sordi ◽  
Angiola Berenzi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Enzymatic Treatment ◽  
Isolated Cells ◽  
Good Manufacturing Practices ◽  
A Cell ◽  
Integrated Device

The adipose tissue is a good source of mesenchymal stromal cells that requires minimally invasive isolation procedures. To ensure reproducibility, efficacy, and safety for clinical uses, these procedures have to be in compliant with good manufacturing practices. Techniques for harvesting and processing human adipose tissue have rapidly evolved in the last years, and Lipogems® represents an innovative approach to obtain microfragmented adipose tissue in a short time, without expansion and/or enzymatic treatment. The aim of this study was to assess the presence of mesenchymal stromal cells in the drain bag of the device by using a prototype Lipogems processor to wash the lipoaspirate in standardized condition. We found that, besides oil and blood residues, the drain bag contained single isolated cells easy to expand and with the typical characteristics of mesenchymal stromal cells that can be loaded with paclitaxel to use for drug-delivery application. Our findings suggest the possibility to replace the drain bag with a “cell culture chamber” obtaining a new integrated device that, without enzymatic treatment, can isolate and expand mesenchymal stromal cells in one step with high good manufacturing practices compliance. This system could be used to obtain mesenchymal stromal cells for regenerative purposes and for drug delivery.

scholarly journals Therapeutic effects of adipose-tissue-derived mesenchymal stromal cells and their extracellular vesicles in experimental silicosis

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Elga Bandeira ◽  
Helena Oliveira ◽  
Johnatas D. Silva ◽  
Rubem F. S. Menna-Barreto ◽  
Christina M. Takyia ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Therapeutic Effects ◽  
Experimental Silicosis

Interaction of human adipose tissue-derived mesenchymal stromal cells with breast cancer cells

Neoplasma ◽  
2011 ◽  
Vol 58 (5) ◽  
pp. 361-370 ◽  
Author(s):  
L. KUCEROVA ◽  
M. KOVACOVICOVA ◽  
S. POLAK ◽  
M. BOHAC ◽  
J. FEDELES ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Adipose Tissue ◽  
Cancer Cells ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Breast Cancer Cells ◽  
Human Adipose Tissue

scholarly journals miRNA Reference Genes in Extracellular Vesicles Released from Amniotic Membrane-Derived Mesenchymal Stromal Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 347 ◽  
Author(s):  
Enrico Ragni ◽  
Carlotta Perucca Orfei ◽  
Antonietta Rosa Silini ◽  
Alessandra Colombini ◽  
Marco Viganò ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Amniotic Membrane ◽  
Stromal Cells ◽  
Reference Genes ◽  
Target Cells ◽  
Human Amniotic Membrane ◽  
U6 Snrna ◽  
Therapeutic Molecules ◽  
The Impact

Human amniotic membrane and amniotic membrane-derived mesenchymal stromal cells (hAMSCs) have produced promising results in regenerative medicine, especially for the treatment of inflammatory-based diseases and for different injuries including those in the orthopedic field such as tendon disorders. hAMSCs have been proposed to exert their anti-inflammatory and healing potential via secreted factors, both free and conveyed within extracellular vesicles (EVs). In particular, EV miRNAs are considered privileged players due to their impact on target cells and tissues, and their future use as therapeutic molecules is being intensely investigated. In this view, EV-miRNA quantification in either research or future clinical products has emerged as a crucial paradigm, although, to date, largely unsolved due to lack of reliable reference genes (RGs). In this study, a panel of thirteen putative miRNA RGs (let-7a-5p, miR-16-5p, miR-22-5p, miR-23a-3p, miR-26a-5p, miR-29a-5p, miR-101-3p, miR-103a-3p, miR-221-3p, miR-423-5p, miR-425-5p, miR-660-5p and U6 snRNA) that were identified in different EV types was assessed in hAMSC-EVs. A validated experimental pipeline was followed, sifting the output of four largely accepted algorithms for RG prediction (geNorm, NormFinder, BestKeeper and ΔCt method). Out of nine RGs constitutively expressed across all EV isolates, miR-101-3p and miR-22-5p resulted in the most stable RGs, whereas miR-423-5p and U6 snRNA performed poorly. miR-22-5p was also previously reported to be a reliable RG in adipose-derived MSC-EVs, suggesting its suitability across samples isolated from different MSC types. Further, to shed light on the impact of incorrect RG choice, the level of five tendon-related miRNAs (miR-29a-3p, miR-135a-5p, miR-146a-5p, miR-337-3p, let-7d-5p) was compared among hAMSC-EVs isolates. The use of miR-423-5p and U6 snRNA did not allow a correct quantification of miRNA incorporation in EVs, leading to less accurate fingerprinting and, if used for potency prediction, misleading indication of the most appropriate clinical batch. These results emphasize the crucial importance of RG choice for EV-miRNAs in hAMSCs studies and contribute to the identification of reliable RGs such as miR-101-3p and miR-22-5p to be validated in other MSC-EVs related fields.

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