Genetic Variability Studies in F1 Seedlings of Cassava (Manihot esculenta Crantz) based on Morphological Traits

ABSTRACTHigh genetic variability is one of important determinants in successful breeding of clonally propagated crops such as cassava. Induced mutation, including mutagenesis using gamma irradiation, is one strategy to increase genetic variability. The objective of this research was to obtain information of lethal doses (LD20-LD50) from five cassava genotypes (Jame-jame, Ratim, UJ-5, Malang-4, and Adira-4), to analyze the variability, and to obtain potentially high yielding cassava mutant candidates. Cuttings of several cassava genotypes were irradiated by 0, 15, 30, 45, and 60 Gy gamma rays. The results showed that genotype Malang-4 had the lowest LD20 (7.53 Gy) and LD50 (18.47 Gy), while genotype Jame-jame had the highest LD20 (24.94 Gy) and LD50 (33.24 Gy). The highest phenotype variability was obtained in the cassava population irradiated by 15-30 Gy gamma rays. The highest phenotype variability was also determined by the source of tissue, i.e., cuttings from the middle stem resulted in the highest phenotype variability. This research found 32 high yielding potential candidate mutants.Keywords: gamma rays, LD20, LD50,mutant, mutation

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Genetic diversity characterization of cassava cultivars (Manihot esculenta Crantz).: I) RAPD markers

Genetics and Molecular Biology ◽

10.1590/s1415-47571998000100018 ◽

1998 ◽

Vol 21 (1) ◽

pp. 105-113 ◽

Cited By ~ 31

Author(s):

Carlos Colombo ◽

Gérard Second ◽

Tereza Losada Valle ◽

André Charrier

Keyword(s):

Genetic Diversity ◽

Multivariate Analysis ◽

Genetic Variability ◽

Manihot Esculenta ◽

Core Collection ◽

Rapd Markers ◽

Manihot Esculenta Crantz ◽

Important Resource ◽

Flour Production

RAPD markers were used to investigate the genetic diversity of 31 Brazilian cassava clones. The results were compared with the genetic diversity revealed by botanical descriptors. Both sets of variates revealed identical relationships among the cultivars. Multivariate analysis of genetic similarities placed genotypes destinated for consumption "in nature" in one group, and cultivars useful for flour production in another. Brazil’s abundance of landraces presents a broad dispersion and is consequently an important resource of genetic variability. The botanical descriptors were not able to differentiate thirteen pairs of cultivars compared two-by-two, while only one was not differentiated by RAPD markers. These results showed the power of RAPD markers over botanical descriptors in studying genetic diversity, identifying duplicates, as well as validating, or improving a core collection. The latter is particularly important in this vegetatively propagated crop.

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Classification of elite cassava varieties (Manihot esculenta Crantz) cultivated in Benin Republic using farmers’ knowledge, morphological traits and simple sequence repeat (SSR) markers

Genetic Resources and Crop Evolution ◽

10.1007/s10722-017-0550-0 ◽

2017 ◽

Vol 65 (2) ◽

pp. 513-525

Author(s):

A. P. Agre ◽

R. Bhattacharjee ◽

I. Y. Rabbi ◽

O. A. Alaba ◽

N. N. Unachukwu ◽

...

Keyword(s):

Ssr Markers ◽

Manihot Esculenta ◽

Simple Sequence Repeat ◽

Morphological Traits ◽

Sequence Repeat ◽

Manihot Esculenta Crantz ◽

Simple Sequence ◽

Benin Republic ◽

Cassava Varieties

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ANÁLISE DA DIVERGÊNCIA GENÉTICA ENTRE ACESSOS DE Manihot esculenta (Crantz) VIA CARACTERES MORFOAGRONÔMICOS

Nativa ◽

10.31413/nativa.v7i1.5575 ◽

2019 ◽

Vol 7 (1) ◽

pp. 01

Author(s):

Huan Hernadez Ramos ◽

Danilo De Lima Gonçalves ◽

Marilene Da Silva Castro ◽

Marco Antonio Aparecido Barelli

Keyword(s):

Genetic Variability ◽

Genetic Divergence ◽

Manihot Esculenta ◽

Euclidean Distance ◽

Starch Content ◽

Germplasm Collection ◽

Root Weight ◽

Manihot Esculenta Crantz ◽

Mato Grosso ◽

Group I

Este estudo teve como objetivo avaliar a diversidade genética entre acessos tradicionais de mandioca da coleção da Universidade do Estado de Mato Grosso - UNEMAT, através de caracteres morfoagronômicos. A coleção está localizada na Empresa Matogrossense de Pesquisa, Assistência e Extensão Rural (EMPAER) de Cáceres. Foram utilizados 21 acessos para a avaliação dos caracteres morfoagronômicos: comprimento do lóbulo foliar; largura do lóbulo foliar; comprimento do pecíolo; altura de plantas; altura da primeira ramificação; comprimento das raízes; diâmetro da raiz; peso da raiz; contagem do número de polpas sadias por planta; número de raízes; peso em água; matéria seca e teor de amido. A análise de dissimilaridade foi baseada na Distância Euclidiana e o agrupamento pelo método Hierárquico UPGMA. Os resultados obtidos com o uso de características morfoagronômicas e da Distância Euclidiana dos 21 acessos de mandioca da coleção da UNEMAT evidenciaram a existência de variabilidade genética, onde a maior similaridade ocorreu entre os acessos Rondônia e N.I. 10 - 0501, e a maior divergência entre os acessos Sinop e Liberatão 0507.O Grupo I alocou o maior número de acessos pelo UPGMA e a característica altura média da primeira ramificação foi determinante na formação do Grupo IV. Os acessos analisados apresentaram divergência genética, onde os descritores analisados foram suficientes para a diferenciação dos acessos, onde os NI 02-2816, Poupa Branca e Sinop foram os mais promissores como parentais para cruzamentos entre os demais acessos.Palavras-chave: produção de mandioca, coleção de germoplasma, variabilidade genética. ANALYSIS OF GENETIC DIVERGENCE AMONG Manihot esculenta (crantz) ACCESS VIA MORPHOAGRONOMIC TRAITS ABSTRACT: This study aimed to evaluate the genetic diversity among accessions of traditional cassava collection at the University of Mato Grosso - UNEMAT through morphoagronomic traits. The collection is located in Mato Grosso Company Research, Assistance and Rural Extension (EMPA) of Cáceres. We used 21 accessions for the evaluation of morphoagronomic traits: length of the leaf lobe; lobe leaf width; length petiole; plant height; height of first branching; length of roots; diameter of the root; root weight; counting the number of healthy pulps per plant; number of roots; weight in water; dry matter and starch content. The dissimilarity analysis was based on Euclidean distance and hierarchical clustering method UPGMA. The results obtained with the use of morphoagronomic traits and the Euclidean distance of 21 cassava accessions from the collection of UNEMAT showed the existence of genetic variability, where the greatest similarity was between the Rondônia and N.I. 10 - 0501, and the greatest divergence among the accessions Sinop and Liberatão 0507. The Group I allocated the highest number of accesses by UPGMA and the characteristic height of the first branch was instrumental in the formation of Group IV. The analyzed accessions presented genetic divergence, where the descriptors analyzed were sufficient for the differentiation of the accesses where NI 02-2816, Poupa Branca and Sinop were the most promising as parental for crossings between the other accessions.Keywords: cassava production, germplasm collection, genetic variability.

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Domestication Syndrome in Cassava (Manihot esculenta Crantz): Assessing Morphological Traits and Differentially Expressed Genes Associated with Genetic Diversity of Storage Root

Cassava ◽

10.5772/intechopen.71348 ◽

2018 ◽

Author(s):

Luiz Joaquim Castelo Branco Carvalho ◽

James V. Anderson ◽

Songbi Chen ◽

Chikelu Mba ◽

Münevver Doğramaci

Keyword(s):

Genetic Diversity ◽

Differentially Expressed Genes ◽

Manihot Esculenta ◽

Morphological Traits ◽

Differentially Expressed ◽

Storage Root ◽

Manihot Esculenta Crantz ◽

Domestication Syndrome

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PROFILING OF GUAVA GENOTYPES GROWN IN VIDARBHA CONDITIONS ON THE BASIS OF MORPHOLOGICAL TRAITS

FLORA AND FAUNA ◽

10.33451/florafauna.v23i1pp37-44 ◽

2017 ◽

Vol 23 (1) ◽

Author(s):

R.A. PATIL ◽

S.G. BHARAD ◽

S.N. SAWANT

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Morphological Traits ◽

Considerable Extent ◽

Fruit Traits ◽

Breeding Programmes ◽

Tree Leaf ◽

Qualitative Characteristics ◽

Intrapopulation Diversity

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.

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