Background: Pyogenic infections can be caused by various microorganisms and may co-exist as poly microbial infections which require
antibiotic therapy. The inappropriate use of antibiotics has led to major problems of MDRO's contributing to morbidity and mortality. Even though
the bacteriological prole of pus samples in many studies remain the same, antimicrobial susceptibility pattern of these isolates has shown a lot
of variation. This study was conducted to assess bacteriological prole of pus samples and their antimicrobial susceptibility pattern
Materials and Methods:Aretrospective study was carried out from April to June 2021. 540 pus culture aerobic bacterial isolates were included.
The samples were cultured on Blood and MacConkey agar. After aerobic incubation at 37oC for 18-24 hrs, organisms were identied by
conventional bacteriology methods as per laboratory protocol and antimicrobial susceptibility was tested by Kirby Bauer disc diffusion method as
per CLSI 2020 guideline.
Results: Out of 540 pus isolates , 452(83.7%)were gram negative bacili and 88(16.3%) were gram positive cocci Pseudomonas spp. 173(31.9%),
was most common gram negative isolate while S.. aureus 66(12.19%) was most common Gram positive isolate. Most of gram negative isolates
were susceptible to Imipenem and Polymyxin and gram positive isolates to vancomycin and linezolid .
Conclusion: To combat resistance irrational use of antibiotics should be avoided. Also regular surveillance helps in implementing better
therapeutic strategies to reduce morbidity and mortality
Antimicrobial Susceptibility Pattern among Aerobic Gram Negative Bacilli of Lower Respiratory Tract Specimens of a Tertiary Care Hospital in Southern Rajasthan, India
