scholarly journals Capsular Typing of Staphylococcus aureus Isolated from Raw Milk Samples using Duplex Polymerase Chain Reaction

2020 ◽  
Vol 9 (11) ◽  
pp. 2044-2050
Author(s):  
A. Khan ◽  
R. Sharma ◽  
R. Grover ◽  
M. Rizwan
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Raw Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction ◽  
Capsular Typing

scholarly journals Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction

2019 ◽  
Vol 12 (1) ◽  
pp. 68-71 ◽  
Author(s):  
Mustofa Helmi Effendi ◽  
Mirza Atikah Madarina Hisyam ◽  
Poedji Hastutiek ◽  
Wiwiek Tyasningsih
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Dairy Farms ◽  
Raw Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Epidemiological Tool ◽  
Gene Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Coa Gene

Aim: This study was conducted to study the coagulase (coa) gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle from three different regions in East Java Province, Indonesia. Materials and Methods: A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of S. aureus. The presumptive isolates were confirmed by coa test. The confirmed S. aureus isolates were subjected to coa gene polymerase chain reaction. Results: Of 160 different samples, 20 (12.5%) isolates of S. aureus were confirmed by positive coa test. Of 20 S. aureus isolates, 19 (95%) isolates carried coa gene. Six different genotypes of coa gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One coa genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region. Conclusion: It can be concluded that coa gene is easily epidemiological tool for detection of variation strain from S. aureus.

scholarly journals Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction

2013 ◽  
Vol 96 (11) ◽  
pp. 6955-6964 ◽  
Author(s):  
B.G. Botaro ◽  
C.S. Cortinhas ◽  
L.V. Março ◽  
J.F.G. Moreno ◽  
L.F.P. Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

scholarly journals Daignosis of Staphylococcus aureus mastitis in bovine in Al-Najaf province by using Polymerase chain reaction (PCR)

2013 ◽  
Vol 12 (2) ◽  
pp. 81
Author(s):  
N. A. Al- Anbagi
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Subclinical Mastitis ◽  
Molecular Method ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Significant Incidence ◽  
Left Posterior ◽  
Polymerase Chain

This study was conducted to collect 388 milk samples from cows at different villages and townships in Al-Najaf province to examine about Staphylococcus aureus mastitis .CMT was used for subclinical mastitis screening ,212(54.6%) milk samples were mastitic .The molecular method (PCR assay) was used to detected the presence (glpF) gene in classically diagnosed S.aureus, which appeared that 38(92.6%) S.aureus mastitis as 13(32.5%) clinical and 25(14.5%) subclinical mastitis .There was high significant incidence of Staphylococcus aureus mastitis in left posterior udder quarter rather than others quarters

scholarly journals Characterization of Campylobacter jejuni An Important Zoonotic Food Borne Pathogen from Mastitis Milk and Raw Milk Samples

2021 ◽  
Author(s):  
Sumedha Bobade ◽  
K. Vijayarani ◽  
K.G. Tirumurugaan ◽  
A. Thangavelu ◽  
S. Vairamuthu
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Morphological Characteristics ◽  
Raw Milk ◽  
Phenotypic Characterization ◽  
Chain Reaction ◽  
Milk Samples ◽  
Food Borne ◽  
Polymerase Chain ◽  
Pcr Targeting

Background: Campylobacter has emerged as an important zoonotic food borne pathogen of human and animals worldwide. Campylobacter is one of the most common bacterial enteropathogens of food borne origin in industrialized countries with C. jejuni being the most common species followed by C. coli. There are very few cases reported from mastitis therefore this study was aimed to determine the incidence of Campylobacter jejuni from from mastitis milk and raw milk samples. Methods: Total of 72 milk samples comprising mastitis milk (20) and raw milk (52) were collected. The samples were subjected to cultural examination, biochemical as well as molecular identification. The isolates were further subjected to phenotypic characterization by biochemical test and genotypic characterization by Polymerase Chain Reaction. The isolates were subjected to PCR targeting hip O and MAP A genes. Result: The 52 samples showed growth on modified Blood Free Charcoal Cefoperazone Deoxycholate agar media and 18 (34.61%) samples showed typical morphological characteristics. The result revealed that 10 (19.23%) isolates were positive by phenotypic characteristic and 7(70%) by Polymerase chain reaction for C. jejuni. The outcome result showed that importance of Campylobacter jejuni in cattle, especially raw milk and milk from mastitis cows, as a potential source for transmission of Campylobacteriosis in human and dairy farm environment. This can cause acute bacterial gastroenteritis in humans and associated with foodborn infection, food safety and a serious public health threat.

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