scholarly journals Effects of wine on cellular and endocellular membranes in vitro

OENO One ◽  
2000 ◽  
Vol 34 (4) ◽  
pp. 177
Author(s):  
Robert Wattiaux ◽  
Kathy Jaworski ◽  
Simone Wattiaux-De Coninck
Keyword(s):  
Free Radicals ◽  
Cell Membrane ◽  
Phospholipase C ◽  
Red Wine ◽  
White Wine ◽  
Lysosomal Membrane ◽  
Red Cell ◽  
Protective Effects ◽  
Red Cell Membrane

<p style="text-align: justify;">Several observations suggest that wine consumption could have beneficial effects on health by preventing cardiovascular diseases. Apparently, ethanol is not the only component responsible for these effects, phenolic substances: tanins, flavonoids may have an important role. As these compounds are able to affect biological membranes, we investigated the effect of wine <em>in vitro</em> on two model membranes: the lysosomal membrane and the red cell membrane. The integrity of rat liver lysosomal membrane was assessed by measuring the latency of Nacetylglucosaminidase, a lysosomal enzyme, when the organelles are subjected to free radicals of oxygen in the presence of different concentrations of wine. The state of red cell membrane was followed by determining hemolysis caused by phospholipase C. Our results show that low concentrations of red wine prevent the deterioration of the membrane of lysosomes induced by oxygen free radicals generated by the xanthine-xanthine oxidase system and oppose hemolysis induced by treating red cells with <em>Clostridium welchii</em> phospholipase C. White wine is considerably less efficient. As similar effects can be obtained with some phenolic compounds, it is probable that the membrane protective effects of red wine that we describe, originate from its content in these substances.</p>

scholarly journals Decreased life span and membrane damage of carbamylated erythrocytes in vitro

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 909-917 ◽  
Author(s):  
TA Lane ◽  
ER Burka
Keyword(s):  
Cell Membrane ◽  
Life Span ◽  
Membrane Damage ◽  
Membrane Lipid ◽  
Red Cell ◽  
Significant Modification ◽  
Red Cell Membrane ◽  
Cell Membrane Damage ◽  
Sickle Erythrocytes

Abstract Red blood cells exposed to cyanate (CNO) in vitro have a concentration- dependent decreased cell survival time associated with an inhibition of the ability of the cell membrane to synthesize lipids. The t1/2 of rabbit erythrocytes exposed to 30 mM or 50 mM cyanate for 1 hr at 37 degrees C is reduced from the normal 24 days to 15 and 9 days, respectively. The cyanate-induced defect in membrane lipid metabolism is irreversible. Carbamylation of membrane proteins and damage to metabolism are minimized by limiting exposure in vitro to 15 mM cyanate at 4 degrees C for 30 min. Cells carbamylated under these conditions do not have a shortened life span. Levels of globin carbamylation of 0.5 moles CNO/mole hemoglobin, shown to be clinically effective in prolonging the life span of sickle erythrocytes, are obtained under these conditions and reach maximal levels after only 30 min of incubation. Carbamylation of blood in CPD anticoagulant is inferior to either ACD or heparin. The findings indicate that adequate carbamylation of sickle erythrocytes with minimal red cell membrane damage can be achieved without significant modification of the standard plasmapheresis procedure utilized by the working blood bank.

scholarly journals Decreased life span and membrane damage of carbamylated erythrocytes in vitro

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 909-917
Author(s):  
TA Lane ◽  
ER Burka
Keyword(s):  
Cell Membrane ◽  
Life Span ◽  
Membrane Damage ◽  
Membrane Lipid ◽  
Red Cell ◽  
Significant Modification ◽  
Red Cell Membrane ◽  
Cell Membrane Damage ◽  
Sickle Erythrocytes

Red blood cells exposed to cyanate (CNO) in vitro have a concentration- dependent decreased cell survival time associated with an inhibition of the ability of the cell membrane to synthesize lipids. The t1/2 of rabbit erythrocytes exposed to 30 mM or 50 mM cyanate for 1 hr at 37 degrees C is reduced from the normal 24 days to 15 and 9 days, respectively. The cyanate-induced defect in membrane lipid metabolism is irreversible. Carbamylation of membrane proteins and damage to metabolism are minimized by limiting exposure in vitro to 15 mM cyanate at 4 degrees C for 30 min. Cells carbamylated under these conditions do not have a shortened life span. Levels of globin carbamylation of 0.5 moles CNO/mole hemoglobin, shown to be clinically effective in prolonging the life span of sickle erythrocytes, are obtained under these conditions and reach maximal levels after only 30 min of incubation. Carbamylation of blood in CPD anticoagulant is inferior to either ACD or heparin. The findings indicate that adequate carbamylation of sickle erythrocytes with minimal red cell membrane damage can be achieved without significant modification of the standard plasmapheresis procedure utilized by the working blood bank.

Influence of red cell membrane on diffusion of oxygen

1960 ◽  
Vol 15 (6) ◽  
pp. 1117-1122 ◽  
Author(s):  
F. Kreuzer ◽  
W. Z. Yahr
Keyword(s):  
Cell Membrane ◽  
Light Transmission ◽  
Hemoglobin Concentration ◽  
Pure Oxygen ◽  
Red Cell ◽  
Freezing And Thawing ◽  
Red Cell Membrane ◽  
Equal Concentration ◽  
Significant Difference

Experiments in vitro were conducted on human blood at 37°C. Centrifuged blood samples were divided into packed red cells and three kinds of hemoglobin solution of equal concentration with the hemolysis effected by freezing and thawing, distilled water and ether. Since the four media had the same hemoglobin concentration, the conditions for the propagation of oxygen were substantially identical, with the only difference that the oxygen had to cross the barrier of the red cell membranes in the case of the cells. All media were analyzed with the thin-layer technique. Light transmission changes resulting from the uptake of pure oxygen by the reduced media were continuously recorded. From such tracings, the one-third and one-half saturation times were evaluated and compared. No significant difference was found between any of the highly concentrated hemoglobin solutions and the packed red cell suspensions. It was concluded that the red cell membrane does not seem to represent an essential impediment to the propagation of oxygen under these circumstances. Submitted on April 20, 1960

Reduction of in Vitro Autohemolysis in Hereditary Spherocytosis by Impermeant Molecules

Blood ◽  
1967 ◽  
Vol 30 (4) ◽  
pp. 449-456 ◽  
Author(s):  
DANIEL N. MOHLER ◽  
Norman Eby
Keyword(s):  
Cell Membrane ◽  
Hereditary Spherocytosis ◽  
Red Cell ◽  
Atp Content ◽  
Red Cell Membrane ◽  
Phosphorylated Compounds ◽  
Osmotic Behavior

Abstract It has been shown that a variety of compounds will reduce the abnormal autohemolysis which occurs after 48 hours of incubation of HS erythrocytes. These compounds either provide energy in the form of ATP, as is the case with glucose and adenosine, or provide an external osmotic force by virtue of their inability to cross the red cell membrane, as is the case with phosphorylated compounds, glutathione and sucrose. None of these latter compounds caused a significant preservation of erythrocyte ATP content. In assessing the effect of added compounds in the autohemolysis test, their osmotic behavior should be taken into account.

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