scholarly journals PREDIÇÃO FÍSICO-QUÍMICA, MODELAGEM E ANÁLISE DO MECANISMO DE INTERAÇÃO DA QUITINASE Mo-chi1 [Moringa oleifera, LAM.], COM POLI-β-(1-4)-N-ACETIL-D-GLUCOSAMINE: UMA ABORDAGEM in silico

2018 ◽  
Vol 5 (1) ◽  
pp. 111-120
Author(s):  
Lara Cristhian Costa Bezerra ◽  
Evaristo Wagner Alves de Queiroz ◽  
José Ednésio da Cruz Freire
Keyword(s):  
In Silico ◽  
Moringa Oleifera ◽  
Plant Genome ◽  
Autodock Vina ◽  
Genome Database ◽  
Chinese Herbal ◽  
Α Helix ◽  
Herbal Plant ◽  
Moringa Oleifera Lam

Quitinases são enzimas capazes de hidrolisar ligações β-(1,4) entre os resíduos de N-acetil-β-D-glucosamina (GlcNAc), presente em diversos organismos.  Elucidar as propriedades físico-químicas da Mo-chi1 identificada na planta Moringa oleifera empregando ferramentas in silico.  Após busca no Chinese Herbal Plant Genome Database, a sequência (ID: 10006495) foi analisada quanto ao peptídeo sinal, propriedades físico-químicas, pontes de sulfeto, domínios, estruturas secundárias, modelagem. O modo de interação entre Mo-chi1 e quitina foi predito com o programa AutoDock Vina.  A Mo-chi1 possui peptídeo sinal com 19 aminoácidos de comprimento (clivado: Ala19 e Ile20), formando polipeptídio maduro de 379 resíduos. A madura possui Mr = 39,56 kDa, pI = 5.44, II = 35.61 e GRAVY = -0.135. Possui uma ponte de sulfeto (5Cys-Cys398). Pertencer à família das GH18 com E-value de 7.43e-153 (CDD) e de 2.3e-90 (SMART). Detém predominância de β-folha (16,85–37%), seguidas de α-Helix (23,3–30,34%) e alças (39,5–52,81%). O estudo de docking molecular mostrou energia favorável a interação entre Mo-chi1 e GlcNAc com E-valor =  -5.9 kcal.mol-1. Embora a Mo-chi1 tenha apresentado propriedades físico-químicas semelhantes a outras quitinases GH18, são necessários estudos mais refinados a fim de identificar seu real potencial.

Inhibition of Echis ocellatus venom metalloprotease by flavonoid-rich ethyl acetate sub-fraction of Moringa oleifera (Lam.) leaves: in vitro and in silico approaches

Toxin Reviews ◽  
2021 ◽  
pp. 1-11
Author(s):  
Akindele Oluwatosin Adeyi ◽  
Kaosarat Keji Mustapha ◽  
Babafemi Siji Ajisebiola ◽  
Olubisi Esther Adeyi ◽  
Damilohun Samuel Metibemu ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
In Silico ◽  
Moringa Oleifera ◽  
Moringa Oleifera Lam

Anti-bacterial efficacy of bio-fabricated silver nanoparticles of aerial part of Moringa oleifera lam: Rapid green synthesis, In-Vitro and In-Silico screening

2021 ◽  
pp. 102229
Author(s):  
Netra Prasad Neupane ◽  
Amit Kumar Kushwaha ◽  
Abhishek Kumar Karn ◽  
Habibullah Khalilullah ◽  
Mohd Masih Uzzaman Khan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
In Silico ◽  
Aerial Part ◽  
Moringa Oleifera ◽  
In Silico Screening ◽  
Moringa Oleifera Lam

Monthly variation and impact of pollution on the nutritional value of Moringa oleifera (Lam.) Leaves

2017 ◽  
Vol 6 (10) ◽  
pp. 5535
Author(s):  
Sachin Umesh Dubey ◽  
Madhu Kanta Kapoor
Keyword(s):  
Vitamin C ◽  
Nutritional Value ◽  
Moringa Oleifera ◽  
Reducing Sugars ◽  
Essential Amino Acids ◽  
Monthly Variation ◽  
Vehicular Pollution ◽  
Total Sugars ◽  
Urban Sewage ◽  
Moringa Oleifera Lam

Moringa oleifera Lam., commonly found tree in sub-Himalayan tracts of India, Pakistan, Bangladesh and Afghanistan has a high nutrition value. The leaves, flowers and immature pods of Moringa are used as a vegetable in many countries. The leaves are highly nutritious and medicinal in nature. They are a rich source of iron, calcium, vitamin A, vitamin C, proteins, and essential amino acids. Hence Moringa leaves can be a good source of protein for the vegetarians and the under-nourished population. Present investigation deals with the study of monthly variation in the nutritional value of Moringa leaves from the month of June 2015 to January 2016. Impact of urban sewage pollution and roadside vehicular pollution on the amount of reducing and total sugars, chlorophylls (a, b and total), proteins, vitamin-C and pH of the leaves, was also studied. Results revealed that the highest amounts of reducing sugars, total sugars, chlorophyll b and total chlorophyll during October 2015 whereas proteins, chlorophyll a, vitamin C and pH were highest during January 2016. Leaf samples collected from all the study sites exhibited minimum amounts of reducing sugars, chlorophylls (a, b and total) and pH during July 2015 whereas total sugars were lowest during December 2015. Proteins and vitamin C values were lowest during August and June 2015 respectively. Sewage and vehicular pollution showed an adverse effect on the nutritional contents of Moringa oleifera leaves. Of the two polluted sites, samples from near the sewage flow showed higher impact.

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