scholarly journals The ethyl acetate fraction of sponge Stylissa carteri decreases viability in HER2+ trastuzumab-resistant breast cancer cells

2019 ◽  
Vol 10 (3) ◽  
pp. 239-245
Author(s):  
Julia Nur Jannah ◽  
Pandji Irani Fianza ◽  
Tenny Putri ◽  
Muhammad Hasan Bashari
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Ethyl Acetate Fraction ◽  
Stylissa Carteri

scholarly journals Cytotoxic Activity and Antiangiogenesis of Extract Fraction of Coccinia grandis (L.) Voigt) Base Towards T47D Breast Cancer Cells and Chorio Allantoic Membrane (CAM) of Chicken Embryo Induced by bFGF

2020 ◽  
Vol 20 (1) ◽  
pp. 37
Author(s):  
Agustinus Alfred Seran ◽  
Jason M Peranginangin ◽  
Rizal M Rukmana
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Chicken Embryo ◽  
Breast Cancer Cells ◽  
Ethyl Acetate Fraction ◽  
Coccinia Grandis ◽  
Extract Fraction

Aktivitas Sitotoksik dan Antiangiogenesis Ekstrak Fraksi Umbi Mentimun Papasan (Coccinia grandis (L.) Voigt) Terhadap Sel Kanker Payudara T47D Dan Chorio Allantoic Membrane (CAM) Embrio Ayam yang Diinduksi bFGFPenelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dan antiangiogenesis ekstrak dan fraksi umbi mentimun papasan (Cocconia grandis (L.) Voigt) terhadap sel kanker payudara T47D dan Chorio Allantoic Membrane (CAM) embrio ayam yang diinduksi protein bFGF. Ekstrak diperoleh melalui metode maserasi dengan pelarut etanol 96%. Ekstrak etanol selanjutnya difraksinasi dengan metode partisi cair-cair. Fraksi yang diperoleh dilakukan analisis kualitatif dengan metode kromatografi lapis tipis (KLT). Dari analisis KLT menunjukan bahwa terdapat beberapa golongan senyawa aktif yaitu alkaloid, flavonoid, tanin, saponin dan terpen. Uji sitotoksik dilakukan menggunakan sel kanker payudara T47D dan sel VERO dengan metode MTT assay kemudian dibaca absorbansinya pada ELISA reader dengan parameter pengamatan nilai IC50. Uji antiangiogenesis dilakukan menggunakan metode Chorio Allantoic Membrane (CAM) embrio ayam yang diinduksi protein bFGF dengan parameter pengamatan jumlah pembuluh darah baru. Hasil penelitian menunjukan bahwa ekstrak, fraksi n-heksan, fraksi etil asetat, dan fraksi air memiliki nilai IC50 berturut-turut adalah 500,167; 353,131; 109,975; dan 303,990 µg/mL. Fraksi etil asetat memiliki nilai IC50 paling kecil sehingga dilanjutkan ke uji antiangiogenesis. Fraksi etil asetat memiliki aktivitas antiangiogenesis terhadap Chorio Allantoic Membrane(CAM) dengan konsentrasi efektif sebesar 219,95 µg/mL ditandai dengan persentase penghambatan angiogenesis sebesar 56,78%.Kata Kunci:  Antiangiogenesis; Chorio Allantoic Membrane (CAM); mentimun papasan (Cocconia Grandis (L.) Voigt); Sitotoksik; T47D Cytotoxic Activity and Antiangiogenesis of  Extract Fraction of Coccinia grandis (L.) Voigt) Base Towards T47D Breast Cancer Cells and Chorio Allantoic Membrane (CAM) of Chicken Embryo Induced by bFGF ABSTRACTThis study aims to identify the cytotoxic activity and antiangiogenesis of extract and fraction of (Coccinia Grandis (L.) Voigt) base towards T47D breast cancer cells and Chorio Allantoic Membrane (CAM) of chiken embryo induced by bFGF. The extract was extracted throught maceration method with ethanol 96% soluble. The ethanol extract was then fractionated by the liquid-liquid partition method. The fractions obtained were analyzed qualitatively by the thin layer chromatography (TLC) method. The TLC analysis shows that there are several classes of active compounds, namely alkaloids, flavonoids, tannins, saponons and terpenes. The ethanol extraction was then fractionated using the liquid-liquid extraction. The cytotoxic test was conducted using the T47D breast cancer cells and VERO cells with the MTT assay method which their absorbance were read on ELISA reader with the value of observation parameters IC50. The antiangiogenesis test was conducted using the Chorio Allantoic Membrane (CAM) method of chicken embryo induced with bFGF protein with the new blood vessels count as the observation parameters. The results showed that the extract, n-hexane fraction, ethyl acetate fraction, and water fraction had IC50 values of 500.167; 353.131; 109.975; and 303.990 µg/mL, respectively. Ethyl acetate fraction has the smallest IC50 value so it is continued to the antiangiogenesis test. Ethyl acetate fraction had an antiangiogenesis activity towards Chorio Allantoic Membrane (CAM) with the effective concentration 219,95 µg/mL marked by the angiogenesis inhibitors percentage of 56,78%.Keywords: Antiangiogenesis; Chorio Allantoic Membrane (CAM); Coccinia grandis (L.) Voigt); cytotoxic; T47D

scholarly journals Correction: Ou-Yang, F. et al. Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima). Int. J. Mol. Sci. 2019, 20, 3238

2021 ◽  
Vol 22 (2) ◽  
pp. 668
Author(s):  
Fu Ou-Yang ◽  
I-Hsuan Tsai ◽  
Jen-Yang Tang ◽  
Ching-Yu Yen ◽  
Yuan-Bin Cheng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Ethyl Acetate Extract ◽  
Published Paper

The authors would like to make corrections to their published paper [...]

scholarly journals Crude ethyl acetate extract of marine microalga,Chaetoceros calcitrans, induces Apoptosis in MDA-MB-231 breast cancer cells

2014 ◽  
Vol 10 (37) ◽  
pp. 1 ◽  
Author(s):  
SuPeng Loh ◽  
Noorjahan BanuMohamed Alitheen ◽  
SuHua Goh ◽  
FatimahMd Yusoff ◽  
SweeKeong Yap
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Ethyl Acetate Extract ◽  
Marine Microalga ◽  
Chaetoceros Calcitrans

scholarly journals EURYCINOID: POTENSI RUMPUT GONG (EURYCAULON CINEREUM) KHAS BANGKA BELITUNG SEBAGAI KANDIDAT SENYAWA ANTIKANKER PAYUDARA BERBASIS BAHAN ALAM

Khazanah ◽  
2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Dian Nida Salsabila ◽  
◽  
Asgar Purnama ◽  
Yoga Febriana ◽  
Hady Anshory ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Vero Cell ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Ethyl Acetate Fraction ◽  
T47d Cell ◽  
Assay Method ◽  
Cell Fractionation ◽  
Chemopreventive Agent ◽  
Vacuum Liquid Chromatography

Traditionally, some People from bangka belitung has consumed Eriocaulon cinereum R.Br as an anticancer drug. Aims of this study is to determine the potential of cytotoxicity of Fraction of Ethyl Acetate and Dichlorometane against T47D cell. Fractionation using Vacuum Liquid Chromatography (VLC) method. MTT assay method was used on evaluating the citotoxic activity. The fraction of dichloromethane performed high activity as a chemopreventive agent with IC50 score was 131,921 µg/ml to T47D and 413,042 µg/ml cells to vero cell whereas for ethyl acetate fraction performed lower activity as chemopreventive agent with IC50 score was 531,808 µg/ml to T47D and 679,114 µg/ml to vero cell). Phytochemical tests showed that the dichloromethane fraction contains phenolic, steroid and terpenoid compounds. While ethyl acetate fraction contains alkaloids, flavonoids and terpenoids. These compounds have activity on cancer cells through apoptotic mechanisms. The results showed the excellent potential of this plant in killing breast cancer cells.

scholarly journals Cytotoxic and Antimetastasis Effect of Ethyl Acetate Fraction from Caesalpinia sappan L. on MCF-7/HER2 Cells

2017 ◽  
Vol 8 (1) ◽  
pp. 42 ◽  
Author(s):  
Riris Istighfari Jenie ◽  
Sri Handayani ◽  
Ratna Asmah Susidarti ◽  
Zalinar Udin ◽  
Edy Meiyanto
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Ethyl Acetate Fraction ◽  
Migration And Invasion ◽  
Her2 Overexpression ◽  
Caesalpinia Sappan ◽  
Mcf 7

Overexpression of HER2 in breast cancer cell is found on invasive breast cancer and correlated with worse prognosis. Caesalpinia sappan L shows cytotoxic activity on various cancer cells. The goal of this research is to determine the cytotoxic activity and inhibition of migration and invasion of ethyl acetate fraction of Caesalpinia sappan L. (FEA) on HER2 overexpression-breast cancer cells (MCF-7/HER2). The MTT and flow cytometry assay showed that FEA revealed cytotoxic effect in a dose-dependent manner (IC50= 34 ± 3.1 µg/ml) and induced apoptosis, S and G2/M phase accumulation. Wound healing assay, gelatin zymography and immunoblotting assay showed that FEA inhibited migration and suppressed MMP2, MMP9, HER2 and Rac1 protein level. Thus, ethyl acetate fraction of Caesalpinia sappan L. is potential to be developed on future research especially to treat metastatic breast cancer with HER2 overexpression.Keywords: Ethyl acetate fraction of Caesalpinia sappan L, cytotoxic effect, migration and invasion, MCF-7/HER2 cells

scholarly journals Antiproliferation activity of God's crown fruit (Phaleria macrocarpa) extract and fractions against MCM-B2 breast cancer cells

2020 ◽  
Author(s):  
Ratna Puspita ◽  
Hasim ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Water Fraction ◽  
Activity Test ◽  
Phaleria Macrocarpa

Breast cancer is a disease caused by abnormal cell proliferation in the breast. God’s crown fruit (Phaleria macrocarpa) and its seed have potential as an antiproliferation of cancer cells. It contains active compounds such as flavonoids, alkaloids, polyphenols, and tannins. The sample of God’s crown fruit was obtained by extraction and fractionation using the maceration method. Cytotoxicity of extracts and fractions was determined using the Brine Shrimp Lethality Test method. The antiproliferation activity test of God’s crown fruit against MCM-B2 was performed using the hemacytometer method. The God’s crown fruit sample consists of crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. Lethal concentration 50 (LC50) values in crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 13.72, 147.55, 405.81, and 149.55 ppm, respectively. The concentration of the test sample was directly used for the antiproliferation activity test on MCM-B2 cells. God’s crown fruit can act as an antiproliferation of MCM-B2. The smallest concentration of those samples has inhibited MCM-B2 cell proliferation which is 3.5 ppm crude ethanol extract lower than 100 ppm doxorubicin. The maximum percentage of the antiproliferation activity of crude ethanol extract (56 ppm) was able to inhibit MCM-B2 cell proliferation by 58.28% while doxorubicin (100 ppm) by 31.2%. This is due to the fact that crude ethanol extract has a lot of complex polar phytochemical content. The crude ethanol extract compounds inhibit MCM-B2 cell proliferation synergistically.

scholarly journals Anticancer Effect of the Ethyl Acetate Fraction from Orostachys japonicus on MDA-MB-231 Human Breast Cancer Cells through Extensive Induction of Apoptosis, Cell Cycle Arrest, and Antimetastasis

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Ji-Hye Kwon ◽  
Jae-Hyeon Kim ◽  
Deok-Seon Ryu ◽  
Hyun-Ji Lee ◽  
Dong-Seok Lee
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Ethyl Acetate Fraction ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Cycle Arrest

The antibreast cancer activities of the ethyl acetate fraction from Orostachys japonicus (OJEF) were investigated in MDA-MB-231 human breast cancer cells through WST assay, DAPI staining, flow cytometry analysis, and western blotting. OJEF effectively inhibited MDA-MB-231 cells by inducing apoptosis via intrinsic, extrinsic, and endoplasmic reticulum (ER) stress response pathways, cell cycle arrest at the G1/S phase, and antimetastasis including inhibition of tight junction, adherens junction, invasion, and migration. The MAPK family-mediated upstream signal transduction through p-p38 and p-ERK was considered to affect the downstream signal transduction including induction of apoptosis, cell cycle arrest, and antimetastasis. In conclusion, we executed an integrated study on the anticancer activities of OJEF, which extensively induced apoptosis, cell cycle arrest, and antimetastasis in estrogen-independent MDA-MB-231 human breast cancer cells known to be liable to metastasize.

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