<p>Daun Kelor (<em>Moringa oleifera</em>) merupakan salah satu tanaman yang berpotensi memiliki aktivitas antikanker. Beberapa penelitian telah melaporkan bahwa daun <em>M. oleifera </em>mengandung sejumlah senyawa bioaktif yang memiliki potensi sebagai antikanker. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik esktrak etanol, fraksi etil asetat dan fraksi n-heksana daun <em>M. oleifera </em>terhadap sel kanker payudara T47D. Daun <em>M. oleifera </em>dimaserasi dengan pelarut etanol 90%. Ekstrak yang diperoleh dipartisi dengan menggunakan pelarut n-heksana dan etil asetat. Masing-masing ekstrak etanol, fraksi etil asetat dan fraksi n-heksana diuji aktivitas sitotoksiknya terhadap sel kanker payudara T47D menggunakan metode MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). Konsentrasi ekstrak dan fraksi yang digunakan adalah berturut-turut: 1500, 750, 375, 187, 93, 46, 50 dan 23 μg/mL dengan waktu inkubasi selama 48 jam. Nilai IC<sub>50 </sub>ekstrak etanol, fraksi etil asetat dan fraksi n-heksana daun <em>M. oleifera</em> berturut-turut yaitu sebesar 51,31; 20,17; 223,67 μg/mL. Berdasarkan hasil tersebut, terlihat bahwa fraksi etil asetat daun <em>M. oleifera </em>memiliki aktivitas<em> </em>sitotoksik yang paling tinggi terhadap sel kanker payudara T47D.</p><p><strong>Cytotoxic Activity of the</strong> <strong>Ethanol Extract, Ethyl Acetate and n-hexane Fractions</strong><strong> of Kelor Leaves (<em>Moringa oleifera</em>) against Breast Cancer Cell T74D. </strong>Moringa oleifera is one plant that has the potential anticancer activity. Several studies have been reported that <em>M.</em><em> oleifera</em> leaves contain bioactives compounds that are potential as anticancer. This study was aimed to determine the cytotoxic activity of the ethanol extract, ethyl acetate and n-hexane fraction of <em>M. oleifera</em> leaf against breast cancer cell T47D. <em>M. oleifera</em> leaves were extracted by maceration with ethanol solvent. The extracts that have been obtained were partitioned by using n-hexane and ethyl acetate solvents. Each ethanol extract, ethyl acetate fraction and n-hexane fraction were tested for their cytotoxic activity against T47D breast cancer cells using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method. The applied concentration of extract and fraction were 1500, 750, 375, 187, 93, 46, 50 and 23 μg/mL with an incubation time of 48 hours. IC<sub>50</sub> value of ethanol extract, ethyl acetate and n-hexane fractions of <em>M. oleifera</em> leaves were 51.31; 20.17; 223.67 μg/mL. Based on these results, it shows that the ethyl acetate fractions of <em>M. oleifera</em>leaves are highly toxic against T47D breast cancer cells.</p><div><div><p> </p></div></div>