Bacteriuria Strip test compare To Standard Culture Method in diabetic patient with Urinary Tract Infection

Background:Bacteriuria during pregnancy has been known to cause many complications like low birth weight and premature delivery.Objective: This study was done to evaluate the diagnostic accuracy of rapid dipstick test to predict urinary tract infection in pregnancy against the gold standard urine culture.Material&Methods: A total of 200 mid stream urine samples were collected from asymptomatic pregnant females. These specimens were cultured in blood agar and MacConkey's agar by using the standard loop technique and incubated aerobically at 37°C overnight. The criterion for clinically significant bacteriuria was either a pure or predominant culture of >105 colony forming units (CFU)/ml. All the specimens were also examined microscopically for pyuria and bacteriuria.Results: The prevalence of asymptomatic bacteriuria in pregnancy was 15 % in our study. The sensitivity and the specificity for leucocyte esterase were 85.7% and 74.4% and for nitrites, they were 64.2% and 72%.Conclusion: The study revealed that use of either leukocyte esterase or nitrite for screening of asymptomatic bacteriuria in pregnancy was associated with many false positive and negative results when compared with the gold standard urine culture method. By using their combination maximum negative predictive value of .98 was achieved.

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Detection of urinary tract infections by rapid methods.

Clinical Microbiology Reviews ◽

10.1128/cmr.1.3.268 ◽

1988 ◽

Vol 1 (3) ◽

pp. 268-280 ◽

Cited By ~ 66

Author(s):

M Pezzlo

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Patient Population ◽

Cost Effective ◽

Culture Method ◽

Diagnostic Laboratory ◽

Patient Groups ◽

Tract Infections ◽

Selection Of

A review of rapid urine screens for detection of bacteriuria and pyuria demonstrates a number of available alternatives to the culture method. Selection of one or more of these systems for routine use is dependent upon the laboratory and the patient population being tested. The laboratory approach to the diagnosis of urinary tract infection should consider the clinical diagnosis of the patient whenever possible. Keeping in mind that quantitative urine cultures alone cannot be used to detect infection in some patient populations unless lower colony counts are considered, a rapid screen may be a more practical approach. It has become accepted that 10(5) CFU/ml can no longer be used as the standard for all patient groups, that pyuria often is important in making the diagnosis of a urinary tract infection, and that most of the rapid screens are more sensitive than the culture method at 10(5) CFU/ml. Presently, no one approach can be recommended for all laboratories and all patient groups. However, each diagnostic laboratory should select one approach which is best for its situation. It is not practical, efficient, or cost effective to define a protocol for each possible clinical condition; however, all should be considered when developing a protocol. This protocol should be compatible with the patient population and communicated to the physicians. Use of a rapid screen should be beneficial to the patient, the physician, and the laboratory.

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P29 Severe renal failure in a diabetic patient with urinary tract infection: The kidney may be in bubbles!

Diabetes Research and Clinical Practice ◽

10.1016/s0168-8227(14)70137-8 ◽

2014 ◽

Vol 103 ◽

pp. S41

Author(s):

F. Ngeugoue Tchokouaha ◽

M. Etoa ◽

G. Ashuntantang

Keyword(s):

Renal Failure ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Diabetic Patient ◽

Severe Renal Failure

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Recto-Vaginal Colonization and Urinary Tract Infection by Group B Streptococcus in the Pregnant Diabetic Patient

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.3109/00016348809004274 ◽

1988 ◽

Vol 67 (7) ◽

pp. 617-620 ◽

Cited By ~ 7

Author(s):

R. Matorras ◽

A. García-Perea ◽

J. A. Usandizaga ◽

F. Omeñaca

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Diabetic Patient ◽

Group B Streptococcus ◽

Vaginal Colonization ◽

Group B ◽

Pregnant Diabetic

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Comparative value of chronic urinary tract infection (UTI) diagnosis between standard culture sensitivity and next generation sequence (NGS) in urine samples

European Urology Supplements ◽

10.1016/s1569-9056(19)30283-0 ◽

2019 ◽

Vol 18 (1) ◽

pp. e378 ◽

Cited By ~ 1

Author(s):

M. Dixon ◽

M. Stefil ◽

C. Skinner ◽

G. Coba ◽

S. Zaman ◽

...

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Urine Samples ◽

Next Generation ◽

Chronic Urinary Tract Infection ◽

Culture Sensitivity ◽

Standard Culture

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Study on microbiology of urinary tract infection and the status of urinary isolates in pregnant women

Medical Journal of Shree Birendra Hospital ◽

10.3126/mjsbh.v12i2.8793 ◽

2015 ◽

Vol 12 (2) ◽

Author(s):

Sharmila Parajuli

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Pregnant Women ◽

Asymptomatic Bacteriuria ◽

Culture Method ◽

First Trimester ◽

Number Of Patients ◽

Sensitivity Pattern ◽

Culture Positive

Background: Urinary tract infection (UTI) is one of the most frequently encountered problems owing to significant number of patients needing hospitalization during pregnancy. The incidence of UTI in pregnant women is reported to be high up to 7-8%. Materials and methods: This is a prospective study conducted in Valley Maternity Hospital during a period of 6 months (Jan 2011 to June 2011). 520 MSU (Mid stream urine samples) from pregnant women clinically suspected of urine infection were evaluated by urine dipstick analysis, microscopic and culture method. The isolates were identified and antibiotic sensitivity pattern was determined by standard protocol. Results: The majority of the patients were in-between the age group of 20-30years- 338cases (65%) and these patients usually presented in the first trimester of pregnancy- 317cases (60.96%).  Out of the 520 clinically suspected UTI cases, 232 (44.61%) was culture positive. Out of the culture positive cases; Escherichia coli (E.coli) was the most common accounting for a total of 144cases (80%). Nitrofurantoin was found to be the most effective drug against the gram negative (Gm-ve) bacteria. Similarly, Ampicillin, Amoxycillin and Cloxacillin were found to be effective agent against gram positive (Gm+ve) bacteria. Conclusion: Screening for bacteriuria is recommended among all pregnant women at the first prenatal visit and in the subsequent trimesters of pregnancy. Prompt treatment of symptomatic UTI and asymptomatic bacteriuria is required in pregnant women to avoid complications like preterm birth, low birth weight and increased perinatal mortality. Key words: UTI, Pregnant women, Antibiotics, Culture method.

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Antimicrobial Sensitivity pattern of Bacteria Causing Urinary Tract Infection among Diabetic Patients

10.21203/rs.2.24215/v1 ◽

2020 ◽

Author(s):

Pratima Thapa ◽

Anita Sunar ◽

Dipendra Lamichanne ◽

Apeksha K.C ◽

Arjan Dhungana ◽

...

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Diabetic Patient ◽

High Rate ◽

Diabetic Patients ◽

Cross Sectional ◽

Antimicrobial Sensitivity ◽

Sensitivity Pattern ◽

Highly Sensitive

Abstract Urinary tract infection is the presence and active multiplication of microorganism within the urinary tract. UTIs are group of infections that affects any part of urinary tract. The cross sectional descriptive study was conducted to determine the antibiotic susceptibility pattern of bacteria causing urinary tract infection in diabetic and non-diabetic patient from February 2016 to March 2016. Among the total 601 urine sample, 250 were diabetics and 351 were non diabetics. All samples were investigated by standard laboratory procedures. Out of diabetic patient 111(44.4%) were female and 139(55.6%) were male and among non-diabetic, 234(66.7%) were female and 117(33.3%) were male. The UTI prevalence rate was found to be 78 (13%) was statistically significant (p = 0.02), among the significant growth 6.8% diabetic and 6.2% non-diabetic. Escherichia coli (54) was the most predominant organism (42.5% in diabetic and 57.5% non-diabetic) followed by Staphylococcus aureus (8). Amikacin, Cotrimoxazole and Nitrofurantoin were most sensitive to E. coli isolated in diabetic and non-diabetic patients among the tested antimicrobials. High rate of resistance was observed with Norfloxacin and Nalidixic acid. Gentamicin, Cefotaxime, Cotrimoxazole and Ciprofloxacin were highly sensitive to S. aureus in diabetic patients while Oxacillin and Azithromycin were resistance and in non-diabetic patient highly sensitive antimicrobials were Azithromycin, Gentamicin, Cefotaxime, Cotrimoxazole, Vancomycin and Ofloxacin while Oxacillin was resistance. The antimicrobial susceptibility testing of bacterial isolates should be performed before the treatment of UTI. Key words : Diabetic, Urinary tract infection, Antimicrobial sensitivity.

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