Establishment of a Cell Suspension Culture of Ageratina Pichinchensis for the Improved Production of Anti-inflammatory Compounds

Many species of the Asteraceae family are used in traditional Mexican medicine for possessing healing properties. Ageratina pichinchensis (Asteraceae) is a plant used for the treatment of gastric ulcers, deep wounds and for its antifungal effects. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran and 3-epilupeol, to evaluate the anti-inflammatory potential of its extracts and perform a phytochemical analysis. Cell suspension cultures were established in MS culture medium supplemented with 30 g L-1 sucrose and 1.0 g L-1 α-naphthaleneacetic acid (NAA) plus 0.1 mg L-1 6-furfurylaminopurine (KIN). The ethyl acetate extracts of cell suspension cultures analyzed by GC revealed that the maximum production of compounds The anti-inflammatory activity of these extracts exhibited significant inhibition of NO production. Furthermore, the phytochemical study of EtOAc and MeOH extracts of the biomass on day 20 led to the identification of 17 known compounds. The structures of compounds were assigned by analysis of 1D and 2D NMR data and the remainder by GC-MS. This is the first report of the production of the (-)-Artemesinol, (-)-Artemesinol glucoside, Encecalin and 3,5-diprenyl-acetophenone compounds by a cell suspension cultures of A. pichinchensis.

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Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Plants ◽

10.3390/plants9101398 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1398

Author(s):

Mariana Sánchez-Ramos ◽

Laura Alvarez ◽

Antonio Romero-Estrada ◽

Antonio Bernabé-Antonio ◽

Silvia Marquina-Bahena ◽

...

Keyword(s):

Suspension Culture ◽

Ethyl Acetate ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Phytochemical Analysis ◽

Naphthaleneacetic Acid ◽

No Production ◽

Phytochemical Study ◽

A Cell ◽

Anti Inflammatory

Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran (2) and 3-epilupeol (3), evaluate the anti-inflammatory potential of its extracts, and perform a phytochemical analysis. Cell suspension cultures were established in a MS culture medium of 30-g L−1 sucrose, 1.0-mg L−1 α-naphthaleneacetic acid, and 0.1-mg L−1 6-furfurylaminopurine. The ethyl acetate extract of the cell culture analyzed by gas chromatography (GC) revealed that the maximum production of anti-inflammatory compounds 2 and 3 occurs on days eight and 16, respectively, improving the time and previously reported yields in callus cultures. The anti-inflammatory activity of these extracts exhibited a significant inhibition of nitric oxide (NO) production. Furthermore, a phytochemical study of the ethyl acetate (EtOAc) and methanol (MeOH) extracts from day 20 led to the identification of 17 known compounds. The structures of the compounds were assigned by an analysis of 1D and 2D NMR data and the remainder by GC–MS. This is the first report of the production of (-)-Artemesinol, (-)-Artemesinol glucoside, encecalin, and 3,5-diprenyl-acetophenone by a cell suspension culture of A. pichinchensis.

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ESTABLISHMENT OF A CELL SUSPENSION CULTURE FROM Calophyllum brasiliense AND EVALUATION OF ITS ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY

Revista Mexicana de Ingeniería Química ◽

10.24275/rmiq/bio476 ◽

2019 ◽

Vol 19 (1) ◽

pp. 59-70

Author(s):

D. Cisneros-Tórres ◽

◽

F. Cruz-Sosa ◽

M.P. Nicasio-Tórres ◽

M. González-Cortazar ◽

...

Keyword(s):

Suspension Culture ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Inflammatory Activity ◽

Calophyllum Brasiliense ◽

A Cell ◽

Anti Inflammatory

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A Study On The Effect of Different Elicitors On Capsaicin Accumulation in Cell Suspension Cultures of Capsicum Assamicum (Bhut Jolokia)

10.21203/rs.3.rs-761917/v1 ◽

2021 ◽

Author(s):

Swet Nisha ◽

Ajitabh Bora ◽

HK Gogoi ◽

SK Dwivedi ◽

PJ Handique

Keyword(s):

Salicylic Acid ◽

Suspension Culture ◽

Methyl Jasmonate ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Sinapic Acid ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Exponential Phase ◽

Bhut Jolokia

Abstract Elicitation of cell suspension cultures of Capsicum assamicum (Bhut Jolokia) for enhancement of capsaicin content was tried using different elicitors such as cellulase, vanillin, methyl jasmonate, salicylic acid and sinapic acid in different concentrations for 24, 48 and 72 hours. Cell suspension culture was established in B5 media supplemented with 3.5 mM 2,4-D (2,4-diphenoxyacetic acid) and 1.1 mM Kin and elicitors were introduced at the end of exponential phase. All the elicitors, except methyl jasmonate, led to significant increase in production of capsaicin. Sinapic acid, when added in 22 µM concentration and incubated for 24 hours, led to highest capsaicin accumulation of 0.5% (5068 µg/g) which was highest among all the treatments.

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Cell suspension culture of Amsonia tabernaemontana Walter: growth, organogenesis and alkaloid production

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1981.083 ◽

2014 ◽

Vol 50 (4) ◽

pp. 615-624 ◽

Cited By ~ 1

Author(s):

Mirosława Furmanowa ◽

Lucyna Rapczewska

Keyword(s):

Suspension Culture ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Cell Suspension Cultures ◽

Cell Number ◽

Growth Increment ◽

Suspension Cultures ◽

Cell Suspensions ◽

Alkaloid Production ◽

Cellular Aggregates

The paper discusses the growth of cell suspension cultures of Amsonia tabernaemontana Walter established from callus of hypocotyl origin. The cell number and growth increment were determined. Cellular aggregates developed well in the Wood and Braun (WB) medium with 1 mg/l NAA and 0.5 mg/l kinetin (growth increment 712.4). When the aggregates were cultured on WB media without NAA and kinetin or with 0.02 mg/l kinetin and 3 mg/l IAA, Toots developed an the aggregates. Examiination of the roots and cell suspensions indicates that the Toots are richer in alkaloids than the callus and cell suspensions.

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Biotransformation of (–)-Caryophyllene Oxide by Cell Suspension Culture of Catharanthus roseus

Zeitschrift für Naturforschung B ◽

10.1515/znb-2006-0214 ◽

2006 ◽

Vol 61 (2) ◽

pp. 197-200 ◽

Cited By ~ 11

Author(s):

Muhammad Iqbal Choudhary ◽

Zafar A. Siddiqui ◽

Saifullah Khan ◽

Syed G. Musharraf ◽

◽

...

Keyword(s):

Suspension Culture ◽

Cell Suspension ◽

Catharanthus Roseus ◽

Cell Suspension Culture ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Spectroscopic Techniques ◽

Caryophyllene Oxide ◽

New Compounds

AbstractCatharanthus roseus cell suspension cultures were employed for the biotransformation of (-)-caryophyllene oxide (1), and four metabolites, 15-hydroxycaryophyllene oxide (2), 4β ,5α- dihydroxycaryophyll-8(13)-ene (3), 2β -hydroxycaryophyllene oxide (4), and 2-hydroxy-4,5- epoxycaryophyllan-13-ol (5) were obtained. Metabolites 4 and 5 were found to be new compounds, and their structures were deduced by different spectroscopic techniques.

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TINJAUAN Penggunaan Suspensi Sel dalam Kultur In Vitro

Jurnal AgroBiogen ◽

10.21082/jbio.v5n2.2009.p84-92 ◽

2016 ◽

Vol 5 (2) ◽

pp. 84 ◽

Cited By ~ 1

Author(s):

Sri Hutami

Keyword(s):

Secondary Metabolites ◽

Suspension Culture ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Mass Production ◽

Large Scale ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Cell Physiology

Cell suspension culture could be defined as a process that allows rapidly dividing homogenous suspension of cells to grow in liquid nutrient media. There are two main types of suspension cultures: (1) Batch cultures in which cells are nurtured in a fixed volume of medium until growth ceases and (2) Continuous cultures in which cell growth is maintained by continuous replenishment of sterile nutrient media. Plant cell suspension cultures are mostly used for the biochemical investigation of cell physiology, growth, metabolism, protoplast fusion, transformation and for large scale production of seed by bioreactor and production of secondary metabolites. Contamination is one of the largest problems when dealing with cell cultures. Differences between the products of cell suspension culture and whole plant are frequently observed. These phenomena’s may be resulted from lack of differentiation and organization and cell cultureinduced variation. Utilization of cell suspension culture in Indonesia is still limited, some of them for mass production of plantation seed with bioreactor system and for production of secondary metabolites. The success of this study give the opportunity for mass production of seeds from other plants and also production of secondary metabolites.

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Establishment of Callus Induction and Cell Suspension Cultures of Dendrathema Indicum var. Aromaticum a Scented Chrysanthemum

Journal of Plant Studies ◽

10.5539/jps.v6n2p38 ◽

2017 ◽

Vol 6 (2) ◽

pp. 38 ◽

Cited By ~ 1

Author(s):

Liyan Jin ◽

Yang Yang ◽

Wenjie Gao ◽

Mingxue Gong ◽

Jijia Wang ◽

...

Keyword(s):

Suspension Culture ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Callus Induction ◽

Time Course ◽

Callus Formation ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Growth Potential ◽

Stem Segments

Dendranthema indicum var. aromaticum is an important aroma plant in genus Dendrathema, and the establishment of callus cultures and cell suspension cultures is the basement of further protoplast fusion studies, which make it possible to breed new fragrant chrysanthemum. In this study, the effects of different plant growth regulating substances in different concentrations on callus induction were investigated with stem segments, leaves, petioles as explants. The results showed that the optimal explants were lower stem segments according to the percentage of callus formation, callus hardness, growth potential and shoot differentiation. The optimal induction mediums were MS supplemented with 1.0 mg．l-12.4 D and 0.2 mg．l-1 6-BA. The cell suspension culture system was established by using the subculture calli. The results showed that the suitable inoculum size was 2g and the suitable cell suspension culture medium was MS supplemented with 0.2 mg．l-1 6-BA and 0.5 mg．l-1 2,4-D. The time course of cell growth showed that the greatest cell fresh weight appeared on day 14 and the highest cell viability on day 3.

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INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

BERITA BIOLOGI ◽

10.14203/beritabiologi.v16i1.2687 ◽

2017 ◽

Vol 16 (1) ◽

pp. 1-11

Author(s):

Aryani Leksonowati ◽

Witjaksono Witjaksono ◽

Diah Ratnadewi

Keyword(s):

Suspension Culture ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Callus Formation ◽

Combined Treatment ◽

Cell Suspension Cultures ◽

Compact Structure ◽

Friable Callus ◽

Aquilaria Malaccensis

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage. The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.

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