scholarly journals Molecular Identification of Endophytic Fungi from Local Rice and Growth Test on Several Types of Culture Media

2019 ◽  
Vol 7 (2) ◽  
pp. 89
Author(s):  
Syamsia Syamsia ◽  
Abubakar Idhan ◽  
Amanda Patappari ◽  
Noerfitryani Noerfitryani ◽  
Rahmi Rahmi ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Plant Tissue ◽  
Culture Media ◽  
Sequencing Analysis ◽  
Coprinopsis Cinerea ◽  
Disease Symptoms ◽  
Growth Test ◽  
Chemical Inputs ◽  
Dna Sequencing Analysis ◽  
Blast Program

Local rice is rice that has been cultivated for generations by the community and commonly cultivated without using chemical inputs. Endophytic fungi are fungi that live in the plant tissue and does not cause disease symptoms in the host plants. This study aimed to molecular identifying isolates of MDTA and MDTB endophytic fungi which have been isolated from the local Pulu Mandoti rice plant tissue and growth test on the four types of culture media those were synthetic PDA, natural PDA, MPA, and MEA. The fungi DNA isolation using DNesay Kit. DNA sequencing analysis using the mega BLAST program showed that the MDTB fungus has similarities to Podoscypha bolleana strain 32034 no accession JQ675334 and Podoscypha bolleana strain 32032 no accession JQ675332, whereas the MDTA fungus has similarities to Coprinopsis cinerea A2S3-5 isolate and Coprinopsis cinerea strain CNRMA / F 07-32. The best culture media and sporulation of endophytic fungi is MPA media. This research is the first study to molecular identifying with endophytic fungi from local rice and viability test on the four types of culture media. The results of this study contribute to the diversity of local rice endophytic fungi in Sulawesi.

scholarly journals Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides

2016 ◽  
Vol 2016 ◽  
pp. 1-4
Author(s):  
Chun Wu
Keyword(s):  
Dna Polymerase ◽  
Click Reaction ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sequencing Analysis ◽  
Abasic Site ◽  
Nylon Membrane ◽  
Abasic Sites ◽  
Polymerase Chain ◽  
Dna Sequencing Analysis ◽  
Endonuclease Digestion

Biotinylation of deoxyguanosine at an abasic site in double-stranded oligodeoxynucleotides was studied. The biotinylation of deoxyguanosine is achieved by copper-catalyzed click reaction after the conjugation of the oligodeoxynucleotide with 2-oxohex-5-ynal. The biotinylation enables visualization of the biotinylated oligodeoxynucleotides by chemiluminescence on a nylon membrane. In order to investigate the biotinylated site, the biotinylated oligodeoxynucleotides were amplified by the DNA polymerase chain reaction. Replacement of guanine opposing the abasic site with adenine generated by the activity of the terminal deoxynucleotidyl transferase of DNA polymerase was detected by DNA sequencing analysis and restriction endonuclease digestion. This study suggests that 2-oxohex-5-ynal may be useful for the detection of the unpaired deoxyguanosine endogenously generated at abasic sites in genomic DNA.

Human c-fms proto-oncogene: comparative analysis with an abnormal allele

1985 ◽  
Vol 5 (2) ◽  
pp. 422-426
Author(s):  
J S Verbeek ◽  
A J Roebroek ◽  
A M van den Ouweland ◽  
H P Bloemers ◽  
W J Van de Ven
Keyword(s):  
Comparative Analysis ◽  
Dna Sequencing ◽  
Sequencing Analysis ◽  
Base Pairs ◽  
Small Deletion ◽  
Viral Oncogene ◽  
Close Proximity ◽  
Genetic Sequences ◽  
Dna Sequencing Analysis

The organization of the human c-fms proto-oncogene has been determined and compared with an abnormal allele. The human v-fms homologous genetic sequences are dispersed discontinuously and colinearly with the viral oncogene over a DNA region of ca. 32 kilobase pairs. The abnormal c-fms locus contains a small deletion in its 3' portion. DNA sequencing analysis indicated that it was 426 base pairs in size and located in close proximity to a putative c-fms exon.

scholarly journals Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 802 ◽  
Author(s):  
Hadad ◽  
Luria ◽  
Smith ◽  
Sela ◽  
Lachman ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Virus Disease ◽  
Middle Eastern ◽  
Sequencing Analysis ◽  
Disease Symptoms ◽  
Next Generation Sequencing Analysis ◽  
Nutrition Deprivation ◽  
Rapid Amplification ◽  
Race Pcr ◽  
Complete Viral Genome

In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the Closteroviridae family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85–99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, Lactuca sativa, and Catharanthus roseus plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV.

scholarly journals Comparison of Angelica Species Roots Using Taste Sensor and DNA Sequencing Analysis

2012 ◽  
Vol 27 (6) ◽  
pp. 37-42 ◽  
Author(s):  
Young Hwa Kim ◽  
Goya Choi ◽  
Hye Won Lee ◽  
Gwan Ho Lee ◽  
Seong Wook Chae ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Sequencing Analysis ◽  
Dna Sequencing Analysis ◽  
Taste Sensor

Comparative View of In Silico DNA Sequencing Analysis Tools

2011 ◽  
pp. 207-221 ◽  
Author(s):  
Sissades Tongsima ◽  
Anunchai Assawamakin ◽  
Jittima Piriyapongsa ◽  
Philip J. Shaw
Keyword(s):  
Dna Sequencing ◽  
In Silico ◽  
Sequencing Analysis ◽  
Analysis Tools ◽  
Dna Sequencing Analysis
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