Influence of Coherent Properties of Water on Biological Growth of Plants

The purpose of the research was to study the effect of Allium cepa L on the growth processes of the coherent properties of various water sources and samples of distilled and natural water with the electrophysical properties of the original samples transferred to them. Materials and methods. The Kirlian gas-discharge glow of water droplets was recorded on an X-ray film. We used histograms of the luminescence corona brightness, calculated the values of the Euclidean distance for the medians of the heights of the histogram columns and for the differences in the medians in adjacent intervals. The values during of the research were compared with similar parameters of pre-formed samples of images of typical waters (distilled, tap water, from natural sources outside and from the territories of monasteries), as well as water samples with an established type and degree of coherence. The objects of the growth test were onion plants – Allium cepa L. The height of the seedlings, the length of the roots were estimated, and their dry weight was determined. Results and discussion. The identity of the signs of coherence in most samples of electrophysical (energy-informational) copies of water from original sources, the correspondence between the results of the growth test and the properties of water coherence were revealed. There was an increase in the biomass of the test culture when grown on samples with dextrorotatory coherence of water as its degree increased. With levorotatory polarization of water coherence, the biomass decreases with an increase in its degree. The study showed the possibility of transferring the electrophysical properties of various samples of natural water both to distilled water and to other water samples from natural sources. We experimentally demonstrated the possibility of transferring the quantum properties of liquid-phase objects for the preparation and use of biologically active substances in agriculture, biology and medicine without their side effects. Conclusion. Kirlian photography of water can be used as an additional way of express-indication of the physical state of the water basins of the biosphere, including the quality of drinking water by their coherent properties. It is perspective to study the effects of water sources with certain coherent properties on the growth processes of certain plant species and their parts

Droplet evaporation patterns of wheat seed leakages differ following treatments with arsenic at ultra-high dilution level

Introduction “Agro-homeopathy” plays an important role in sustainable agriculture providing cost-saving and residue-free plant treatments for the improvement of yield and the management of diseases and pests [1]. However, one of the main difficulties in “agro-homeopathy” is the exact choice of the right remedy and dilution, which requires the performance of many time-consuming trials. The droplet evaporation method could constitute a screening method for evaluating the efficacy of extremely low doses and high dilutions on plants. Droplet patterns prepared out of wheat seed leakages show differences in their complexity in relation to the seed vigour [2]. Here we show that this structure-forming capacity of seed leakages prepared in water or in ultra-high diluted (UHD) solutions may give a reliable picture of the treatment’s stimulating or inhibiting influence on plant vitality. Materials and Methods Part of the seeds was stressed with 0.1% As2O3 (As) aqueous solution [3]. The droplet patterns were prepared out of four samples: non-stressed (ns) seeds in water, ns-seeds in As 45x, stressed (s) seeds in water, s-seeds in As 45x. The experiment was performed in a 3 day repetition, 3 replicates per day, and 5 droplets per each replicate. The resulting patterns were evaluated by means of the ImageJ software [4] for their local connected fractal dimension (LCFD) values. The plant vigour of the four samples was evaluated by means of in vitro wheat growth [5, 6]. Results The mean LCFD of patterns deriving from s-seeds was significantly lower (1.4) vs. ns-seeds (1.7). Moreover, for both ns- and s-seeds, the LCFD was significantly higher with treatment As 45x vs. water (1.8 vs. 1.7 for ns-seeds, and 1.5 vs. 1.2 for s-seeds). The results of the growth test showed a significant vigour decrease caused by As stress (shoot length 19.7mm) vs. ns-seeds (22.4mm); a significant stimulating effect following As 45x treatment was observed for both s-seeds (25.1mm) and ns-seeds (31.4). Discussion The droplet patterns seem to be a sensitive tool for visualizing the effectiveness of UHD on seeds. The experiment showed that the pattern complexity increased with the application of the treatment for both ns- and s-seeds. The growth test provided a further verification of these results. Conclusions Further confirmations are required, but even as it is now the droplet evaporation method may be considered a promising tool for “agro-homeopathic” screening tests. Authors declare that there is no conflict of interest and that this study received no funding. References Betti L, Trebbi G, Majewsky V, Scherr C, Shah-Rossi D, Jaeger T, Baumgartner S. Use of homeopathic preparations in phytopathological models and in field trials: a critical review. Homeopathy 2009; 98: 244-266. Kokornaczyk MO, Dinelli G, Marotti I, Benedettelli S, Nani D, Betti L. Self-organized crystallization patterns from evaporating droplets of Common wheat grain leakages as a potential tool for quality analysis. TheScientificWorldJ. 2011; 11: 1712-1725. Brizzi M, Elia V, Trebbi G, Nani D, Peruzzi M, Betti L. The efficacy of ultra molecular aqueous dilutions on a wheat germination model as a function of heat and aging-time. e-CAM 2011; Article ID 696298: 11p. doi: 10.1093/ecam/nep217. Collins TJ. ImmageJ for microscopy. BioTechniques. 2007; 43: 25-30. Betti L, Brizzi M, Nani D, Peruzzi M. Effect of high dilutions of Arsenicum album on wheat seedlings from seed poisoned with the same substance. Br. Homeopath. J. 1997; 86: 86-89. Brizzi M, Lazzarato L, Nani D, Borghini F, Peruzzi M, Betti L. A biostatistical insight into the As2O3 high dilution effects on the rate and variability of wheat seedling growth. Forsch. Komplementmed. 2005; 12: 277-283.

LAMBEDA PHAGE

Background: Recognition of bacteriophages in many aspects plays an important role such as controlling the number and variation of bacteria and participation in horizontal gene transfer, which is an important process in bacterial evolution. Bacteriophages use small proteins to take over the host molecular machinery and thus interfere with central metabolic processes in infected bacteria. In general, phages inhibit or reverse host transcription to transcribe their genome. Mechanical and structural studies of phage host transcription may lead to the development of new antibacterial agents. Result: The result shows that phage vB_Eco4M-7 must be a lytic virus. This was confirmed by monitoring faglitic development by a one-step growth test. In addition, phage of relatively small uniform plaques (1 mm in diameter) occur without lysogenesis. Electron microscopic analysis showed that vB_Eco4M-7 belongs to the family Myoviridae. Based on mass spectrometric analysis, including the fragmentation pattern of unique peptides, 33 vB_Eco4M-7 phage proteins are assigned to the annotated reading frames. The results indicate that the phage studied is a potential candidate for phage treatment and / or food protection against E. coli, which produce Shiga toxin, as most of these strains belong to the O157 serotype. Conclusion: In general, phages inhibit or reverse host transcription to transcribe their genome. Mechanical and structural studies of phage host transcription may lead to the development of new antibacterial agents. The result shows that phage vB_Eco4M-7 must be a lytic virus. This was confirmed by monitoring faglitic development by a one-step growth test.

UTILIZATION OF ORGANIC WASTE BIOMAS AGAINST GROWTH TEST AND RESULTS OF SAWI CROPS

The abundant condition of organic waste in urban areas of Surabaya requires a solution not only regarding the process of compost, but also how to utilize the biomass organic waste in a framework to socialize the use of urban yard narrow land into a form of cultivation of vegetables that contribute to the nutrition for the family. This potential will be synergistic with Surabaya city government policy related to urban yard Empowerment program that inspires the idea of how to utilize organic waste biomass into something useful for plant growth and reduce the accumulation of excessive waste and odor that is not delicious for the people of Surabaya. By implementing the appropriate technology to utilize waste biomass of organic waste into a material of burial or organic fertilizer after through the process of composting or fermentation to become organic fertilizer that is beneficial for plants. The purpose of this research is to know the influence of organic waste biomass from the results of the posting of urban organic waste against the growth test and the results of mustard crops. Based on the research results, it can be concluded as follows: 1. There is a significant influence of the composition of urban garbage organic fertilizer against the growth parameters and results of the mustard crops in the variables studied, including: length of the plant, the number of leaves, the length of the roots and fresh weight per plant. 2. The value of fresh weight yield per highest crop is achieved by P3 treatment (15% from the weight of planting media) by 313.82 grams and effective and efficient treatment, it is also supported by growth variables such as plant length, number of leaves and root length; Even though statistically different P3 treatments are not significant with the P5 treatment (25%) and P7 (35%). Keywords: organic waste Biomas, mustard greens.

SCC Growth Prediction for Surface Crack in Welded Joint Using Advanced FEA

Management of plant service life is a key issue for improving the safety of light water reactors. In boiling water reactor (BWR) plants, some incidents of intergranular stress corrosion cracking (IGSCC) of components in contact with the main coolant, such as a shroud support weld, have been reported in the past. When a crack is detected in a nuclear component, crack growth analysis is required as part of assessing the structural integrity of that component. In Japan, the “Rules on Fitness-for-Service for Nuclear Power Plants” of the Japan Society of Mechanical Engineers Code (JSME FFS Code) provides a simplified crack growth analysis method, assuming a semi-elliptical crack shape and calculating crack growth at the deepest and surface points of the crack. It is known, however, that the actual shape of the SCC is very likely to be different from a semi-ellipse due to the complex distribution of residual stress and the dependency of crack growth properties on the materials composing the welded joint, particularly in the case of a crack crossing the fusion line. Therefore, crack growth analysis techniques using finite element analysis (FEA) have recently been developed to analyze the crack growth behavior of a crack having a more detailed, natural shape. In crack growth analysis using the FEA technique, natural crack growth behavior in the materials composing the welded joint is estimated by using the reference curves of SCC. These reference curves are based on crack growth test data that are generally obtained with C(T) specimens prepared for homogeneous material. To demonstrate the applicability and adequacy of the analysis technique, it is important to validate the results of crack growth analysis using FEA. In this study, validation of the results of SCC crack growth analysis using FEA was conducted by comparison with the results of a SCC growth test. For the SCC growth test, a groove welded joint consisting of austenitic stainless steels was prepared. The welded joint was subjected to sensitizing heat treatment at 620°C for 24 h. A Lee-James specimen with a surface crack, whose crack plane crossed the fusion line between base and weld metals, was taken from the welded joint. After pre-cracking in air and in high-temperature water, the SCC crack growth test was conducted in a normal water chemistry (NWC) environment. Observation of the fracture surface revealed a transition from transgranular (TG) cracking to intergranular (IG) or interdendritic (ID) cracking, and broader IG morphology was observed on the base metal rather than on the weld metal due to a difference in the SCC growth sensitivity of both materials. On the other hand, a FEA model of the Lee-James specimen was prepared and SCC crack growth with a natural shape was analyzed by using FEA. Here, the observed crack front transitioning from TG to IG/ID was set as the initial crack front of the analysis. The reference curves of SCC growth rate for sensitized type 304 stainless steel and low carbon stainless steel in the JSME FFS Code were applied to the base metal and the weld metal of the specimen, respectively. A comparison of the analysis results with the fracture surface of the actual specimen confirmed that the analyzed crack shape generally showed good agreement with the observed crack shape on the fracture surface, although the analysis conservatively overestimated crack growth around surface points of the crack. Modification of the crack growth analysis was also investigated to obtain more accurate analysis results including around surface points of the crack.

Perbandingan Variasi Media Alternatif dengan Berbagai Sumber Karbohidrat Terhadap Pertumbuhan Candida albicans

In the growth of microorganisms such as fungi, it is necessary to grow a medium that can provide nutrients and as an energy source. Other alternative carbohydrate sources include potatoes, breadfruit, sago, and cassava, which is a distinctive and easy to find food companion. This type of research is descriptive with cross sectional design that aims to determine the difference in alternative media variation from various sources of carbohydrate to Candida albicans. The samples were then made to be the treatment for the creation of alternative media from various carbohydrate sources that further carried out the growth test against Candida albicans. The results of the study are the average growth of colonies on alternative media from Potato carbide sources, namely 655 colonies, breadfruit 1380 colonies, cassava 862 colonies, 372 sago colonies, and semi-synthetic media as a control of 874 colonies. This indicates there is growth of Candida albicans on all alternative media so that it can be used as an alternative medium of fungi growth, as well as the best growth of Candida albicans found in the media Breadfruit Dextrose Agar. Keywords: alternative media, carbohydrate sources, Candida albicans