Aktivitas Antioksidan dan Kandungan Fenolik Total Daun Zodia (Evodia suaveolens)

Senyawa dengan radikal bebas merupakan senyawa yang mempunyai satu atau lebih elektron tidak berpasangan yang dapat merusak sel dan jaringan jika terakumulasi dalam tubuh manusia. Antioksidan dapat berfungsi untuk menginaktivasi reaksi oksidasi dan mencegah terbentuknya radikal tetapi antioksidan sintetik dapat menyebabkan karsinogen. Oleh karena itu, antioksidan yang terkandung dalam sumber alami perlu diteliti lebih lanjut. Salah satunya adalah tanaman zodia (Evodia suaveolens). Tujuan dari penelitian ini adalah untuk mengetahui nilai aktivitas antioksidan dari ekstrak metanol, etil asetat, dan diklorometana dari daun tanaman zodia yang dinyatakan dengan IC 50 (inhibitory concentration) dan untuk mengetahui kadar fenolik total dari ekstrak daun zodia. Serbuk daun zodia dimaserasi menggunakan tiga pelarut, yaitu metanol, etil asetat, dan diklorometana secara terpisah. Penentuan aktivitas antioksidan dilakukan dengan uji penangkapan radikal DPPH (2,2-difenill-1-pikrilhidrazil) sedangkan penentuan kandungan fenolik total dilakukan menggunakan metode spektrofotometri. Nilai aktivitas antioksidan yang dinyatakan dengan IC 50 dari ekstrak metanol, etil asetat, dan diklorometana dari daun zodia berturut-turut adalah 170, 124, dan 409 ppm. Kadar fenolik total dari ekstrak metanol, etil asetat, dan diklorometana dari daun zodia berturut-turut adalah 0,02430; 0,01183; dan 0,00930 mg GAE/g eks. Antioxidant Activity and Total Phenolic Content of Zodia Leaves (Evodia suaveolens). The compounds with free radicals are compounds that have one or more unpaired electrons which can damage cells and tissues if they was accumulated in the human body. Antioxidants can act to inactivate oxidation reactions and prevent the formation of radicals but synthetic antioxidants can cause carcinogens. Therefore, antioxidants containing in natural sources need to be investigated. One of them is the zodia (Evodia suaveolens) plant. The purpose of this study was to determine the value of antioxidant activity of methanol, ethyl acetate, and dichloromethane extract from the leaves of the zodia plant in IC 50 (inhibitory concentration) and to determine the total phenolic content of zodia leaf extract. Zodia leaves powder was macerated using three solvents, namely: methanol, ethyl acetate, and dichloromethane, separately. Determination of antioxidant activity was carried out by DPPH (2,2-diphenyl-1-picrilhydrazyl) radical catching test and the determination of total phenolic content was carried out using spectrophotometric methods. The antioxidant activity values expressed by IC 50 from methanol, ethyl acetate, and dichloromethane extracts from zodia leaves were 170, 124, and 409 ppm, respectively. Total phenolic concentrations of methanol, ethyl acetate, and dichloromethane extracts from zodia leaves were 0.02430, 0.01183, and 0.00930 mg GAE/g.

Download Full-text

PENENTUAN TOTAL FENOLIK DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DARI TEPUNG PELEPAH AREN (Arenga pinnata)

JURNAL ILMIAH SAINS ◽

10.35799/jis.16.2.2016.14067 ◽

2016 ◽

Vol 16 (2) ◽

pp. 68

Author(s):

Yudhie E Prasetyo ◽

Meiske S Sangi ◽

Audy D Wuntu

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Ic50 Value ◽

Dpph Radical Scavenging

PENENTUAN TOTAL FENOLIK DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DARI TEPUNG PELEPAH AREN (Arenga pinnata) ABSTRAK Tujuan penelitian ini untuk menentukan total fenolik dan aktivitas antioksidan fraksi etil asetat dari tepung pelepah aren (Arenga pinnata). Tepung pelepah aren diekstraksi maserasi menggunakan etanol hasil redestilasi cap tikus kemudian di partisi menggunakan pelarut petroleum eter, etil asetat dan aquades. Penentuan kandungan total fenolik menggunakan metode Folin-Ciocalteu dan aktivitas antioksidan menggunakan metode serapan radikal 1,1-difenil-2-pikrilhidrazil (DPPH). Didapati kandungan total fenolik fraksi etil asetat sebesar 172.857 ± 4.762 mg/kg dan aktivitas penangkal radikal bebas fraksi etil asetat sebesar 90.573 µg/mL. Hasil ini menunjukan bahwa fraksi etil asetat berpotensi sebagai antioksidan alami. Kata kunci: Tepung Pelepah aren, fenolik, aktivitas antioksidan, DPPH DETERMINATION TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY ETHYL ACETATE FRACTION OF AREN’S MIDRIB POWDER (Arenga pinnata) ABSTRACT This research aims to determine the total phenolic content and antioxidant activity from ethyl acetate fraction of Aren’s midrib powder (Arenga pinnata). Aren’s midrib powder extracted with maceration method with cap tikus redistillated ethanol and then partitioned with solvents such as petroleum ether, ethyl acetate and distillate water. The determination of phenolic content was using Folin-Ciocalteu method and the antioxidant activity was done using diphenylpycrylhydrazyl (DPPH) radical scavenging. Total phenolic value of ethyl acetate fraction is 172.857 ± 4.762 and IC50 value of ethyl acetate fraction is 90.573 µg/mL. This result shows ethyl acetate fraction as natural antioxidant potential. Keywords: Aren’s midrib powder, phenolic, antioxidant activity, DPPH

Download Full-text

Determination of Carotenoids, Total Phenolic Content, and Antioxidant Activity of Arazá (Eugenia stipitataMcVaugh), an Amazonian Fruit

Journal of Agricultural and Food Chemistry ◽

10.1021/jf205347f ◽

2012 ◽

Vol 60 (18) ◽

pp. 4709-4717 ◽

Cited By ~ 35

Author(s):

G. Astrid Garzón ◽

Carlos-Eduardo Narváez-Cuenca ◽

Rachel E. Kopec ◽

Andrew M. Barry ◽

Kenneth M. Riedl ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic

Download Full-text

Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC)

Jurnal Riset Kimia ◽

10.25077/jrk.v12i2.383 ◽

2021 ◽

Vol 12 (2) ◽

Author(s):

Azimatur Rahmi ◽

Tika Afriani ◽

Linda Hevira ◽

Wike Widiawati

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Activity Assay ◽

Ic50 Value

The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Sembung (Blumea balsamifera L.) is a plant that has the potential as an antioxidant because it contains secondary metabolites of alkaloids, steroids, flavonoids, saponins, and phenolic compounds. In this study, antioxidant activity was tested by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for free radical scavenging activity assay. Evaluation of total phenolic content was tested by using the Folin-Ciocalteau method. The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 valueof 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm. The ethyl acetate fraction of Sembung leaves has a total phenolic content of 161.101 mg GAE/g. This study provided that Blumea balsamifera leaves possess antioxidant.

Download Full-text

Determination of the Effects of Variables on Total Phenolic Content and Antioxidant Activity of Apple Ectracts by Response Surface Method

Journal of the Institute of Science and Technology ◽

10.21597/jist.606913 ◽

2020 ◽

pp. 1014-1022

Author(s):

Recep PALAMUTOĞLU ◽

Cemal KASNAK

Keyword(s):

Antioxidant Activity ◽

Response Surface ◽

Response Surface Method ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Surface Method

Download Full-text

Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

Jurnal Pendidikan Kimia ◽

10.24114/jpkim.v13i2.26984 ◽

2021 ◽

Vol 13 (2) ◽

pp. 137-142

Author(s):

Kasta Gurning ◽

◽

Sifikal Lumbangaol ◽

Risanti F. R. Situmorang ◽

Saronom Silaban ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Extraction Process ◽

Total Phenolic ◽

Betel Leaf ◽

Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

Download Full-text

A paper-based device for simultaneous determination of antioxidant activity and total phenolic content in food samples

Talanta ◽

10.1016/j.talanta.2019.02.048 ◽

2019 ◽

Vol 198 ◽

pp. 542-549 ◽

Cited By ~ 13

Author(s):

Chanoknan Puangbanlang ◽

Kitima Sirivibulkovit ◽

Duangjai Nacapricha ◽

Yupaporn Sameenoi

Keyword(s):

Antioxidant Activity ◽

Simultaneous Determination ◽

Total Phenolic Content ◽

Phenolic Content ◽

Food Samples ◽

Total Phenolic

Download Full-text

Characterization of Eucalyptus, Chestnut and Heather Honeys from Portugal Using Multi-Parameter Analysis and Chemo-Calculus

Foods ◽

10.3390/foods7120194 ◽

2018 ◽

Vol 7 (12) ◽

pp. 194 ◽

Cited By ~ 13

Author(s):

Ioannis Karabagias ◽

Miguel Maia ◽

Vassilios Karabagias ◽

Ilias Gatzias ◽

Anastasia Badeka

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Solid Phase ◽

Parameter Analysis ◽

Total Phenolic ◽

Activity Parameter ◽

In Vitro Antioxidant Activity

The present study was conducted to evaluate the quality and bio-functional properties of Portuguese honeys of different botanical and geographical origins. Quality parameter analyses included the determination of palynological (predominant, secondary, minor and isolated pollen percentage), physicochemical (°Brix, moisture content, pH, electrical conductivity, free acidity, total dissolved solids, salinity, vitamin C content and specific weight) including colour-metrics (CIELAB, Pfund and colour intensity determinations), along with volatile compounds identification using solid phase micro-extraction coupled to gas chromatography mass spectrometry. Bio-activity parameter analysis included the determination of in vitro antioxidant activity and total phenolic content using the 2,2-diphenyl-1-picryl-hydrazyl and Folin-Ciocalteu assays, respectively. Melissopalynological analysis showed that Portuguese honeys were classified as eucalyptus, chestnut and heather, recording significant variations (p < 0.05) among physicochemical, volatile and bio-activity parameter analyses according to both: botanical and geographical origin. Based on the multi-parameter analysis data Portuguese honeys could be characterized by a distinctive colour, a characteristic aroma, whereas conform to the European legislation relating to honey identity and quality. Specific attention should be given in the case of heather honey which showed the highest in vitro antioxidant activity and total phenolic content. Parameters that were also highly correlated using bivariate statistics.

Download Full-text

Phytochemical and antioxidant activity of Cadaba farinosa Forssk stem bark extracts

Physical Sciences Reviews ◽

10.1515/psr-2020-0088 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Emmanuel M. Halilu ◽

Abdullahi M. Abdurrahman ◽

Sylvester N. Mathias ◽

Chinenye J. Ugwah-Oguejiofor ◽

Muntaka Abdulrahman ◽

...

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Free Radical ◽

Ethyl Acetate ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Stem Bark ◽

Free Radical Scavenging ◽

Total Phenolic

Abstract Cadaba farinosa is used in traditional medicine for treatment of cancer, diabetes, and rheumatism. The research was aimed at evaluating the phytochemical and antioxidant activity of the extracts. The powdered stem bark was extracted successively with the aid of Soxhlet extractor using n-hexane, ethyl acetate, and methanol. The resulting extracts were concentrated on rotary evaporator and the percentage yields were calculated. The phytochemical and TLC profiles of the extracts were studied. The antioxidant activity of the extracts and ascorbic acid (standard) were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. The total phenolic content of the extracts and tannic acid (standard) were evaluated using Folin–Ciocalteu reagent. The percentage yields of n-hexane, ethyl acetate, and methanol extracts were found to be 1.19, 1.37, and 13.93%, respectively. The phytochemical screening revealed the presence of tannins, flavonoids, saponins, cardiac glycosides, alkaloids, and triterpenoids. The TLC profiles of the extracts revealed the presence of compounds as evidenced from their R f values. The total phenolic content of ethyl acetate and methanol extracts were found to be 135 and 112 mg, respectively. The free radical scavenging activity demonstrated by the extracts was comparable to ascorbic acid. The ethyl acetate extract had higher phenolic content and demonstrated the highest free radical scavenging with IC50 31.07 mg/mL. The results of research have provided strong preliminary evidence of antioxidant activity which may be because of phenolic compounds in the extracts.

Download Full-text