scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE

Biomedika ◽  
2011 ◽  
Vol 3 (2) ◽  
Author(s):  
Ika Trisharyanti Dian Kusumowati ◽  
Rosita Melannisa ◽  
Kartikaning Ratri
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Positive Correlation

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds,  avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds

Chemical characterization of ethanolic extract of Butterfly pea flower (Clitoria ternatea)

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 127-134
Author(s):  
T.N.M. Tuan Putra ◽  
M.K. Zainol ◽  
N.S. MohdIsa ◽  
N. MohdMaidin
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Light Exposure ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Clitoria Ternatea ◽  
Dpph Method ◽  
Butterfly Pea

Clitoria ternatea, commonly known as Butterfly pea flower or Bunga telang, is a tropical flower and notable as one of the most vital sources of polyphenols with high antioxidant capacity. C. ternatea abundantly produces anthocyanin compounds benefiting natural blue colourants. To date, a blue colourant is the most difficult to obtain since the anthocyanins responsible for this colour is the least stable and easily degraded after extraction. Their stability commonly relies on several operational parameters, such as shifted pH value, high temperature, and light exposure. Apart from colour attributes, anthocyanins have also been identified as a source of functional molecules due to antioxidant activity and beneficial health effects such as anti-cancer, anti-obesity, antimicrobial and more. This study aimed to characterize the chemical constituents of the extractable polyphenols from ethanolic extract of C. ternatea quantitatively. The numerical data were evaluated using the Minitab version 18 statistical method. The chemical profiling, including total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins (TMA) were determined using standard methods. The antioxidant activity was evaluated using DPPH and ABTS methods. The results showed that the ethanolic extract of C. ternatea had 35.7 mg QE/g of TFC, 102.4 mg GAE/g280nm and 28.8 mg GAE/g750nm of TPC and 2.7 CE/g and 2.9 ME/g of TMA. The total phenolic content of C. ternatea showed a good correlation with the antioxidant activity by the DPPH method when analysed using Pearson correlation and showed an increasing trend of antioxidant power with regards to TPC in both assays. In a nutshell, this study contributes to the knowledge of ethanolic extract of C. ternatea. Future research could consider exploring other extraction methods that could enhance the stability of polyphenols particularly the anthocyanins from degradation such as using surfactants.

scholarly journals Determination of total phenolic content and antioxidant activity of fruit mix from Malino, Gowa Regency, South Sulawesi

2021 ◽  
Vol 911 (1) ◽  
pp. 012085
Author(s):  
Risma Eva Cahyanti ◽  
Yusminah Hala ◽  
A. Mu’nisa
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Passion Fruit ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Purple Passion Fruit

Abstract This study aimed to determine the total phenolic content and antioxidant activity of single fruit extracts and mixtures of tomatoes (Solanum lycopersicum), purple passion fruit (Passiflora edulis var. Sims), and strawberries (Fragaria sp.). Tomatoes, purple passion fruit and strawberries were extracted using 96% ethanol as solvent using the maceration method. Determination of total phenolic content using the Folin-ciocalteu method, measurement of antioxidant activity using the DPPH method (1.1 diphenyl-2-picrylhydrazil) spectrophotometrically and measuring the degree of acidity (pH) using a pH meter. Data were analyzed using ANOVA with Tukey’s further test. The results showed that the total phenolic content of tomatoes, purple passion fruit and strawberries before and after mixing were tomato extract (1,731 mg GAE/g), purple passion fruit extract (1,577 mg GAE/g), strawberry extract (1,917 mg GAE)./g), tomato and purple passion fruit extract (1,758 mg GAE/g), tomato and strawberry extract (2,020 mg GAE/g), strawberry and purple passion fruit extract (1,924 mg GAE/g) and tomato, purple passion fruit extract and strawberries (2.107 mg GAE/g). The antioxidant activity showed that there was a significant difference between the purple passion fruit treatments (78.695%), tomatoes and strawberries (86.160%) and tomatoes, purple passion fruit and strawberries (88.328%), but not significantly different from the tomatoes (80.683 %), tomatoes and purple passion fruit (82,059 %) as well as the treatment of strawberries (83.690 %), strawberries and purple passion fruit (84.097 %), but significantly different from the BHA control (93.526 %). It can be concluded that the total phenolic content and antioxidant activity of the mixed extracts of the three fruits, namely tomatoes, purple passion fruit and strawberries, were higher than those of the single fruit extracts without mixing.

scholarly journals Total Phenolic Content, Antioxidant and Antibacterial Activities of Sargassum polycystum of Ethanol Extract from Waters of Kabung Island

2021 ◽  
Vol 9 (3) ◽  
pp. 139
Author(s):  
Ikha Safitri ◽  
W. Warsidah ◽  
Mega Sari Juane Sofiana ◽  
Arie A. Kushadiwijayanto ◽  
T. Novi Sumarni
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Antioxidant Vitamin ◽  
Dpph Method

Sargassum policystum is one of the genus Sargassum from the brown macroalgae group which has an abundance of chemical constituents of fucoidan, fucoxanthin, phenolic acids and alginate which have important biological activities, such as antioxidants and antibacterials. This species thrives in the waters of Kabung Island, Bengkayang, West Kalimantan and has not been exploited so that it tends to act as a marine weed. This study aimed to determine the total phenolic content of the ethanolic extract of S. policystum and its antioxidant and antibacterial activity. Determination of the total content of phenolic compounds using the Folin Ciocalteu method, antioxidant testing using the DPPH method (2,2-diphenyl-1-picrylhydrazyl) and the indicator of sample color change with the addition of these radicals was measured by UV-Vis spectrophotometer. Antibacterial testing was carried out by the test sample diffusion method and the inhibition of the growth of the test bacteria Staphylococcus aureus and Escherichia coli as test bacteria was indicated by the formation of a clear area around the sample wells. The results showed that the phenol content of the ethanolic extract of S. policystum was 79.8 mg/g extract, its antioxidant activity (IC50) was 98.903 ppm, classified as a strong antioxidant, while the comparison antioxidant, Vitamin C, had an IC50 of 6.26 ppm. The inhibitory ability of the ethanol extract against the test bacteria used was indicated by the formation of the largest inhibition diameter on S. aureus of 12.4 mm and E. coli of 12.0 mm.

scholarly journals Total Phenolic Content of Ethanol Extract of Artrocarpus camansi Leave and its Effect to Superoxide Dismutase (SOD) Level in Mice

2018 ◽  
Vol 8 (3) ◽  
pp. 101
Author(s):  
Hendri Asrin ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Marianne Marianne
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Free Radicals ◽  
Total Phenolic Content ◽  
Staining Method ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Control Group ◽  
Dpph Method

A free radical is one of the triggers of degenerative diseases that become the biggest cause of death. Excessive production of free radicals can be neutralized by antioxidant. Antioxidants can be generated from within the body (intracellular). One of them by the enzyme SOD (superoxide dismutase). However, when the production of free radicals exceeds the ability of intracellular antioxidants to neutralize it, antioxidants from outside (extracellular) is necessary. The ethanol extract of Artocarpus camansi leaves (EEACL) contains phenolic compounds which has very strong antioxidant activity based on in vitro study using the DPPH method, but the in vivo study about the total phenolic content effect of its leaves toward antioxidant activity has not been done. 25 mice were divided into 5 groups consisting of control group, a group was induced by stress and three groups were induced by stress, but given EEACL with each dose of 50 mg/kg bw, 100 mg/kg bw and 150 mg/kg bw. Inducing stress in the form of psychological stress was carried out for 7 days and continued with the EEACL administration for 7 days. The mice were dissected and the livers were isolated, then the liver morphological was examined using Hematoxyllin Eosin (HE) staining method and SOD level was examined with immunohistochemical staining method. The data were analyzed with One Way ANOVA and Duncan test using SPSS program version 19.0. Total phenolic content of EEACL is 235.03 ± 4.306 mg GAE/ g of sample. The average SOD levels in the control group is 94.05 %, stressed group is 55.94 %, stress with EEACL dose 50 mg/kg bw group is 58.40 %, stress with EEACL dose 100 mg/kg bw group is 79.68 %, stress with EEACL dose 150 mg/kg bw group is 80.90 %. Based on statistical result, SOD level increased along with the increase of EEACL dose, but not significantly (p < 0,05). Total phenolic content of EEACL has an influence to SOD levels. SOD level increased along with the increase of EEACL administration dose. The higher dose of EEACL leading to higher levels of SOD in the mouse liver.Keywords : total phenolic content, superoxide dismutase, antioxidant, Artocarpus camansi

scholarly journals Correlation of Phenolic Content of Multiflora Honey from Malang and Alastuwo to Activity Antioxidant Using DPPH (2.2-Diphenyl-1-Pickrylhydrazyl)

2021 ◽  
Vol 4 (1) ◽  
pp. 1-11
Author(s):  
Ahwan Abdul
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Activity Test ◽  
Dpph Method ◽  
Color Reagent ◽  
Qualitative Test

Honey contains various compounds that function as antioxidants, one of which is phenolic compounds. The purpose of this study was to determine the correlation between the phenolic content of multiflora honey from Malang and Alastuwo on antioxidant activity using the DPPH method (2,2-Diphenyl-1-picrylhydrazyl). Qualitative test of phenolic compounds with FeCl3 color reagent, determination of total phenolic content by UV-Vis spectrophotometry using Folin-Ciocalteu method and antioxidant activity test using DPPH method (2,2-Diphenyl-1-picrylhydrazyl). The results showed that qualitatively Malang and Alastuwo honey contained phenolic compounds. The total phenolic content of Alastuwo honey was 0.0278 ± 0.0010 mg GAE/g and Malang honey was 0.0301 ± 0.0004 mg GAE/g. The results of the antioxidant activity test showed that Alastuwo honey had weak antioxidant activity (IC50 of 393.37 ± 10.28 ppm), Malang honey had moderate antioxidant activity (IC50 of 217.20 ± 6.61 ppm) and vitamin C had strong antioxidant activity. (IC50 is 2.22 ± 0.19 ppm). The total phenolic content of Alastuwo and Malang honey correlates with its antioxidant activity.

scholarly journals Antioxidant Activity of Sunflower (Helianthus Annuus l.) Ethanolic Extract with DPPH Method and Determination of Total Phenolic and Flavonoid Levels

2021 ◽  
Vol 4 (1) ◽  
pp. 31-42
Author(s):  
Salwa Salwa
Keyword(s):  
Antioxidant Activity ◽  
Helianthus Annuus ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Helianthus Annuus L ◽  
A Value ◽  
Dpph Method

Sunflower (Helianthus annuus L.) is believed to have antioxidant properties. Currently, there are many studies examining compounds that have benefits as antioxidants and are safe for health. However, the sunflower only examined the antioxidants in sunflower seeds. This study aims to determine the antioxidant activity of ethanol extract of flowers, bark, and leaves of sunflowers. The antioxidant activity test using the DPPH method showed the highest antioxidant activity was found in the leaf extract (Helianthus annuus L.) with an IC50 value of 48.841 ppm. The highest phenolic content was found in leaf samples with a value of 35.149 mg gallic acid equivalent/gram extract and the highest determination of flavonoid levels was found in leaf samples with a value of 10.917 mg quercetin equivalent/gram extract.

scholarly journals Determination of Minerals, Total Phenolic Content, Flavonoids, Antioxidants and Antimicrobial Activities of Ethanolic Extract of Sweet Lupinus angustifolius of Palestine

2019 ◽  
pp. 1-6 ◽  
Author(s):  
Michel Hanania ◽  
Sireen Radwan ◽  
Suhair Abu Odeh ◽  
Abeer Qumber
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Ferrous Ion ◽  
Total Phenolic ◽  
Sodium Potassium ◽  
Ethanol Extracts

Aims: To establish the most suitable extraction method for sweet lupine seeds and to determine minerals, phenolic content, flavonoids, antioxidant activity and antimicrobial activities. Study Design: Known and standard experimental procedures are employed. Place and Duration of Study: Department of Chemistry, Bethlehem University- Palestine, from January 2019 to March 2019. Methodology: Seeds were ground and extracted by Soxhlet extractor using ethanol with different percentages (50%, 60%, 70%, 80% and 95%). Sodium, potassium and ferrous ion content were determined. Resistance to bacteria was performed against Escherichia coli and Staphylococcus aureus, while antioxidant activity was determined by FRAP method. Two types of flavonoids were measured: Flavonones and dihydroflavonols via the reaction with 2,4-dinitrophenylhydrazine. Phenolics were determined by the Folin-Ciocalteu method. Results: 50% ethanol resulted in the highest extract residue (18.6%) while 70% and 60% showed the lowest content (10.0% for both). 80% ethanol extracted sample showed the highest content for sodium (56.51 mg Na/g extract), while 60% and 50% ethanol extracts showed the highest content of potassium (2.25 and 2.33 mg K/g extract, respectively). The maximum concentration of ferrous ion was obtained with 70% ethanol (6.854 mg Fe+2/g extract). 95% ethanolic extract showed the highest antioxidant activity (20.24 mg FeSO4/g extract). Similar results were obtained for total phenolic content and flavonoids: 24.60 mg gallic acid/g extract for phenolics and 116.02 mg rutin/g extract for flavonoids. Extracts showed no bacterial activity against both types of bacteria used. Conclusion: 95% ethanol extracted samples showed the highest antioxidant activity and the highest flavonoids and phenolic content. Sweet lupine extract did not perform any antimicrobial activity against both Gram positive and Gram negative bacteria.

scholarly journals Optimization of Natural Antioxidants Extraction from Pineapple Peel and Their Stabilization by Spray Drying

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1255
Author(s):  
Sofia C. Lourenço ◽  
Débora A. Campos ◽  
Ricardo Gómez-García ◽  
Manuela Pintado ◽  
M. Conceição Oliveira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Spray Drying ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Food Products ◽  
Total Phenolic ◽  
Dry Extract ◽  
Pineapple Peel ◽  
Dpph Method

Pineapple peel still contains an important amount of phenolic compounds and vitamins with valuable antioxidant activity. In this way, the aim of this study was the recovery of the bioactive compounds from pineapple peel using environmentally friendly and low-cost techniques, envisaging their application in food products. From the solid-liquid extraction conditions tested, the one delivering an extract with higher total phenolic content and antioxidant capacity was a single extraction step with a solvent-pineapple peel ratio of 1:1 (w/w) for 25 min at ambient temperature, using ethanol-water (80–20%) as a solvent. The resulting extract revealed a total phenolic content value of 11.10 ± 0.01 mg gallic acid equivalent (GAE)/g dry extract, antioxidant activity of 91.79 ± 1.98 µmol Trolox/g dry extract by the DPPH method, and 174.50 ± 9.98 µmol Trolox/g dry extract by the FRAP method. The antioxidant rich extract was subjected to stabilization by the spray drying process at 150 °C of inlet air temperature using maltodextrin (5% w/w) as an encapsulating agent. The results showed that the antioxidant capacity of the encapsulated compounds was maintained after encapsulation. The loaded microparticles obtained, which consist of a bioactive powder, present a great potential to be incorporated in food products or to produce bioactive packaging systems.

scholarly journals Changes of antioxidant activity in honey after heat treatment

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 601-606 ◽  
Author(s):  
G. Šarić ◽  
K. Marković ◽  
D. Vukičević ◽  
E. Lež ◽  
M. Hruškar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Significant Difference ◽  
Acacia Honey ◽  
Different Temperatures ◽  
Ferric Reducing Antioxidant Power

We determined how the antioxidant activity and total phenolic content of honey changed after being subjected to a high temperature. Antioxidant activity was determined using two methods &ndash; FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Total phenolic content was determined by modified Folin-Ciocalteu method. The research was conducted on 31 samples of acacia honey and 8 samples of chestnut honey. All measurements were done at two temperatures &ndash; at 23&deg;C (room temperature) and after 5 min of heating at 95&deg;C. The obtained results show uneven changes of antioxidant activity and total phenolic content among individual samples, i.e. in some samples antioxidant activity decreased after heating, while in others it increased. The same applies to the total phenolic content. Statistical analysis of the results (t-test) showed no statistically significant differences between the results measured at two different temperatures (P &gt; 0.05) in all three methods used, and in both types of honey. The only statistically significant difference (P &lt; 0.05) was observed when using DPPH method in acacia honey.

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