scholarly journals Uji Efektivitas Anthelmintik Ekstrak Etanol Daun Kemangi (Ocimum americanum L.) Terhadap Cacing Gelang (Ascaris lumbricoides) Secara In Vitro

2021 ◽  
Vol 6 (3) ◽  
pp. 254
Author(s):  
Suhrah Febrina Karim ◽  
Nurfiddin Farid ◽  
Hilmiati Wahid ◽  
Musdalifa Musdalifa
Keyword(s):  
Ascaris Lumbricoides ◽  
Ocimum Americanum

Penyakit kecacingan merupakan salah satu penyakit infeksi yang disebabkan oleh masuknya parasit berupa telur cacing kedalam tubuh manusia melalui saluran pencernaan manusia karena adanya pencemaran melalui tanah. Penelitian ini bertujuan untuk mengetahui efek anthelmintik ekstrak etanol daun kemangi (<em>Ocimum americanum </em>L.) terhadap cacing gelang <em>Ascaris lumbricoides</em> dan untuk mengetahui konsentrasi ekstrak etanol daun kemangi (<em>Ocimum americanum </em>L.) yang efektif sebagai anthelmintik terhadap cacing gelang (<em>Ascaris lumbricoides).</em> Penelitian ini merupakan metode eksperimental dengan rancangan acak lengkap. Daun Kemangi di lakukan ekstraksi dengan metode maserasi kemudian dilakukan skrining fitokimia. Hewan uji dibagi menjadi 5 kelompok yaitu Kelompok 1 (ekstrak daun kemangi dosis 10%), Kelompok 2 (ekstrak daun kemangi dosis 20%), Kelompok 3 (ekstrak daun kemangi dosis 30%), Kelompok 4 Kontrol Positif (pirantel pamoat 5 mg/ml) dan Kelompok 5 Kontrol Negatif (NaCl 0,9%). Pengamatan dilakukan tiap 1 jam setelah perendaman sampai semua cacing mati. Hasil penelitian menunjukkan 100% kematian cacing yaitu ekstrak etanol daun kemangi Konsentrasi 10% pada jam ke 24, Konsentrasi 20% pada jam ke 16, dan Konsentrasi 30% pada jam ke 8, Kelompok kontrol positif pada jam ke 2, Kontrol negatif (larutan NaCl 0,9%) pada jam ke 76. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak etanol daun kemangi (<em>Ocimum americanum </em>L.) konsentrasi 30% memberikan efek anthelmintik terhadap cacing gelang (<em>Ascaris lumbricoides)</em> pada jam ke 8.

scholarly journals Effects of anthelminthic of ethanol extract of basil (Ocimum americanum) leaf against Ascaris suum death in vitro

2011 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
OKKIE MHARGA SENTANA ◽  
SRI HARYATI ◽  
YUL MARIYAH
Keyword(s):  
Linear Regression ◽  
Ascaris Suum ◽  
Linear Regression Analysis ◽  
Negative Relationship ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Control Group ◽  
Ocimum Americanum

Sentana OM, Haryati S, Mariyah Y. 2011. Effects of anthelminthic of ethanol extract of basil (Ocimum americanum) leaf against Ascaris suum death in vitro. Biofarmasi 9: 1-6. This research aimed to examine the relationship between the ethanol extract of basil leaf against Ascaris suum mortality rate in vitro. This study was a laboratory experiment with a research design of the post-test with controlled group design. The samples were Ascaris suum Goeze that still actively moved and obtained from pig intestines. The sampling technique used was a purposive sampling by equating the type and the length of worms, and it did not distinguished for the sex of worms. The subjects were divided into five groups, each group consisted of 5 worms and repeated in 6 times. The control group contained 25 mL of physiological saline solution and 25 mL pyrantel pamoate 5 mg/mL. Three other groups contained basil leaves extracts consisted of various concentrations, i.e. 30%, 40% and 50%. The number of dead worms was counted every 2 hours until all the worms in the group died. The data were analyzed by using a linear regression analysis and a probit analysis. Simple linear regression statistical test showed a significance correlation at 0.000 with 0.837 correlation coefficient was negative, R square 0.701, in which the significant correlation <0.05 or the value of 37.565 with a significance of means, there was a negative relationship between the length of time of death of worms with large concentrations of basil extract. Analysis using a probit analysis obtained LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours. The ethanol extract of basil leaf could affect the death of A. suum in vitro with LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours.

Identifikasi Antiinflamasi Partisi Metanol, n-Heksan dan Ekstrak Etanol Daun Kemangi (Ocimum Americanum L.) Secara In Vitro

2021 ◽  
Vol 1 ◽  
pp. 820-828
Author(s):  
Annas Pamening ◽  
W Wirasti ◽  
S Slamet ◽  
Urmatul Waznah
Keyword(s):  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hypotonic Solution ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Red Blood Cell Membranes ◽  
Ocimum Americanum

AbstractBasil plant (Ocimum americanum) is efficacious as an anti-inflammatory and analgesic activity. According to research by Sarma and Babu, 2011, Verma and Kothiyal, 2012 showed basil activity as an antioxidant, antimicrobial, anti-diabetic, anthelmintic, antifungal, insecticide, anti-inflammatory, analgesic, and lowering total cholesterol and LDL-C levels. The purpose of this study was to determine the stabilization activity of red blood cell membranes on methanol partitioning, n-hexane partitioning and ethanol extract of basil leaves in vitro. This study used the erythrocyte membrane stabilization method from the induction of a hypotonic solution with samples of methanol partitioning, n-hexane partitioning and ethanol extract to be compared with a positive control, namely Na diclofenac. By analyzing the data using UV-Vis spectrophotometry test. These results were supported by the ANOVA statistical test which stated that there was a difference in each treatment and continued with the Tukey test which stated that there was no difference between 100 ppm diclofenac sodium and 400 ppm ethanol extract.Keywords: Extract, Basil (Ocimum americanum) Leaf, In Vitro. AbstrakTumbuhan Kemangi (Ocimum americanum) berkhasiat sebagai aktivitas sebagai anti-inflamasi dan analgesik. Menurut penelitian Sarma dan Babu, 2011.,Verma dan Kothiyal, 2012 menunjukkan aktivitas kemangi sebagai antioksidan, antimikroba, anti diabetes, antihelmintik, antifungi, insektisida, antiinflamasi, analgesic, dan menurunkan kadar total kolesterol dan LDL-C. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas stabilisasi membran sel darah merah pada partisi metanol, partisi n-heksan dan ekstrak etanol daun kemangi secara in vitro. Penelitian ini menggunakan metode stabilisasi membran eritrosit dari induksi larutan hipotonik dengan sampel partisi metanol, partisi n-heksan dan ekstrak etanol yang akan dibandingkan dengan kontrol positif yaitu Na diklofenak. Dengan analisis data menggunakan uji spektrofotometri UV-Vis. Hasil ini didukung dengan uji statistik ANOVA yang menyatakan terdapat perbedaan pada setiap perlakuan dan dilanjutkan uji tukey yang menyatakan tidak ada perbedaan pada natrium diklofenak 100 ppm dengan ekstrak etanol konsentrasi 400 ppm.Kata Kunci : Ekstrak, Daun Kemangi (Ocimum americanum), In Vitro.

scholarly journals UJI EFEK ANTELMINTIK EKSTRAK ETANOL DAUN PINANG YAKI (Areca vestiaria) TERHADAP CACING GELANG (Ascaris lumbricoides) SECARA IN VITRO

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 457
Author(s):  
Tirza Roring ◽  
Herny E. I. Simbala ◽  
Edwin De Queljoe
Keyword(s):  
Cell Membrane ◽  
Ascaris Lumbricoides ◽  
Endemic Species ◽  
Ethanol Extract ◽  
Control Group ◽  
Whitney Test ◽  
Post Test ◽  
Ethanol Extracts ◽  
Anthelmintic Effect

ABSTRACT Pinang Yaki (Areca vestiaria) is an endemic species of Sulawesi palm, which has unique characteristics that have been used as medicine , to treat various. Ethanol extracts of pinang yaki leaves contains tannin compounds that can inhibit the work of enzymes, interfere with digestive metabolic processes, and damage the worm cell membrane. This study aims to determine the anthelmintic effect of ethanol extract of pinang yaki leaves obtained from the Tomohon area against roundworms (Ascaris lumbricoides). The sample was extracted by maceration method using ethanol solvent. The test used ethanol extracts f pinang yaki leaves with a concentration of 5%, 10%, and 20%, respectively. The worms were incubated at a temperature of 37ºC, and examined for 24 hours at 6 hour intervals. The number of lysis worms and paralysis was recorded every 6 hours and the data were analyzed using the Kruskal Wallis test and continued using the Mann Whitney test. The results showed an anthelmintic effect of ethanol extracts of pinang yaki leaves on roundworms (Ascaris lumbricoides) in vitro.Keywords         : Anthelmintic, Ethanol extracts of areca leaf, Areca vestiaria, Ascaris lumbricoides  ABSTRAK Pinang Yaki (Areca vestiaria) merupakan jenis palem endemik Sulawesi yang memiliki karakteristik yang unik telah dimanfaatkan sebagai obat, untuk mengobati berbagai penyakit. Ekstrak etanol daun pinang yaki mengandung senyawa tanin yang dapat menghambat kerja enzim, mengganggu proses metabolisme pencernaan, serta merusak membran sel cacing. Penelitian ini bertujuan untuk mengetahui adanya efek antelmintik dari ekstrak etanol daun pinang yaki yang diperoleh dari daerah Tomohon terhadap cacing gelang (Ascaris lumbricoides). Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol. Metode penelitian ini ialah eksperimen laboratorium dengan desain post test only control group. Pengujian menggunakan ekstrak etanol daun pinang yaki dengan konsentrasi 5%, 10%, dan 20%. Cacing diinkubasi pada suhu 37ºC, dan diteliti selama 24 jam dengan interval waktu 6 jam. Jumlah cacing lisis dan paralisis dicatat setiap 6 jam dan selanjutnya data dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan menggunakan uji Man Whitney. Hasil penelitian menunjukkan adanya efek antelmintik dari ekstrak etanol daun pinang yaki terhadap cacing gelang (Ascaris lumbricoides) secara in vitro. Kata kunci      : Antelmintik, Ekstrak Etanol Daun Pinang Yaki, Areca vestiaria, Ascaris lumbricoides

In vivo and in vitro studies on the sonographical detection of Ascaris lumbricoides

1997 ◽  
Vol 27 (3) ◽  
pp. 226-229 ◽  
Author(s):  
Harald Hoffmann ◽  
Michael Kawooya ◽  
Philippe Esterre ◽  
Voahangy E. Ravaoalimalala ◽  
Jens Roth ◽  
...  
Keyword(s):  
Ascaris Lumbricoides ◽  
In Vitro Studies
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