scholarly journals ANTIUROLITHIC ACTIVITY OF BERBERIS TRIFOLIATA EXTRACT ON INDUCED UROLITHIASIS IN RATS BY ZINC DISC IMPLANTATION

2017 ◽  
Vol 15 (1) ◽  
pp. 168
Author(s):  
Raymundo Alejandro Pérez-Hernández ◽  
Silvia Guadalupe Treviño-Moreno ◽  
Gilberto Arévalo- Martínez ◽  
Eduardo Sánchez -García ◽  
Catalina Leos-Rivas ◽  
...  
Keyword(s):  
Kidney Stone ◽  
Methanolic Extract ◽  
Stone Formation ◽  
Final Weight ◽  
The Difference ◽  
Postsurgical Recovery ◽  
Kidney Stone Formation ◽  
Treatment Of Urolithiasis ◽  
Different Doses

Background: In clinical therapy, there is no satisfactory drug available for treatment of urolithiasis, especially for the prevention of their recurrence. The aim of this work was to evaluate in vivo antiurolithic activity of methanolic extract of Berberis trifoliata leaves. Material and methods: Urolithiasis was induced in Wistar rats by zinc disc implantation in urinary bladder. Upon postsurgical recovery, different doses of the methanolic extract of B. trifoliata leaves (50, 100 and 150 mg/kg body weight) were administered orally to zinc disc implanted rats for a period of 20 days. Antiurolithiatic activity was evaluated by measuring the difference between the weight of the implanted zinc discs at the time of implantation and the final weight of the dried calculi taken out from the bladder at the end of the 20 days period of treatment. Results: Extract of B. trifoliata significantly reduced calculi deposition around the implanted zinc disc at all doses (50, 100, and 150 mg/kg). Conclusion: Treatment with methanolic extract of B. trifoliata is useful agent against the kidney stone formation.

scholarly journals Effect of theCopaifera langsdorffiiDesf. Leaf Extract on the Ethylene Glycol-Induced Nephrolithiasis in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Rejane Barbosa de Oliveira ◽  
Eduardo Barbosa Coelho ◽  
Marina Rezende Rodrigues ◽  
Ana Rita de Mello Costa-Machado ◽  
João Paulo Barreto de Sousa ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Kidney Stone ◽  
Biochemical Parameters ◽  
Leaf Extract ◽  
Stone Formation ◽  
Osteopontin Expression ◽  
Copaifera Langsdorffii ◽  
Kidney Stone Formation ◽  
Different Doses

The potential of theCopaifera langsdorffiileaves extract to prevent stone formation was analyzed by means of an ethylene glycol (EG) animal model of nephrolithiasis and anin vitrocrystallization assay. Different doses of theC. langsdorffiileaves extract were administered to rats treated with EG. Urine biochemical parameters were quantified. CaOx deposits count and analysis of osteopontin expression were conducted on kidneys fixed in formalin. Thein vitroassay was performed by turbidimetry. Phytochemical analyses of the extract were accomplished by HPLC-UV-DAD, and several compounds were isolated.C. langsdorffiileaf extract was able to avoid stone formation. The number of deposits was50.30±31.29at the higher extract dose, compared to the value of179.5±45.96achieved with the EG control. Significantly lower oxalate levels and OPN expression and increased citrate levels were observed after extract administration. In thein vitroassay, the extract diluted the formed crystals. Phytochemical analyses showed that the extract is rich in phenolic compounds that are capable of preventing stone formation. Thus, on the basis of our results, we suggest that theC. langsdorffiileaf extract has potential application in the prevention of kidney stone formation.

2086 RENAL MACROPHAGES COULD ENGLOBE CALCIUM OXALATE CRYSTALS DURING KIDNEY STONE FORMATION IN VITRO AND IN VIVO MODELS

2012 ◽  
Vol 187 (4S) ◽  
Author(s):  
Atsushi Okada ◽  
Takahiro Yasui ◽  
Kazumi Taguchi ◽  
Yasuhiko Hirose ◽  
Kazuhiro Niimi ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Kidney Stone ◽  
Stone Formation ◽  
Calcium Oxalate Crystals ◽  
In Vivo Models ◽  
Oxalate Crystals ◽  
Kidney Stone Formation

scholarly journals Multicolor imaging of calcium-binding proteins in human kidney stones for elucidating the effects of proteins on crystal growth

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yutaro Tanaka ◽  
Mihoko Maruyama ◽  
Atsushi Okada ◽  
Yoshihiro Furukawa ◽  
Koichi Momma ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Kidney Stones ◽  
Calcium Binding ◽  
Kidney Stone ◽  
Stone Formation ◽  
Human Kidney ◽  
In Vivo Effects ◽  
Kidney Stone Formation

AbstractThe pathogenesis of kidney stone formation includes multi-step processes involving complex interactions between mineral components and protein matrix. Calcium-binding proteins in kidney stones have great influences on the stone formation. The spatial distributions of these proteins in kidney stones are essential for evaluating the in vivo effects of proteins on the stone formation, although the actual distribution of these proteins is still unclear. We reveal micro-scale distributions of three different proteins, namely osteopontin (OPN), renal prothrombin fragment 1 (RPTF-1), and calgranulin A (Cal-A), in human kidney stones retaining original mineral phases and textures: calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD). OPN and RPTF-1 were distributed inside of both COM and COD crystals, whereas Cal-A was distributed outside of crystals. OPN and RPTF-1 showed homogeneous distributions in COM crystals with mosaic texture, and periodically distributions parallel to specific crystal faces in COD crystals. The unique distributions of these proteins enable us to interpret the different in vivo effects of each protein on CaOx crystal growth based on their physico-chemical properties and the complex physical environment changes of each protein. This method will further allow us to elucidate in vivo effects of different proteins on kidney stone formation.

The controversial association of gut and urinary microbiota with kidney stone formation

2021 ◽  
pp. 105257
Author(s):  
Narjess Bostanghadiri ◽  
Pardis Ziaeefar ◽  
Fatemeh Sameni ◽  
Mohammad Mahmoudi ◽  
Ali Hashemi ◽  
...  
Keyword(s):  
Kidney Stone ◽  
Stone Formation ◽  
Urinary Microbiota ◽  
Kidney Stone Formation
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