scholarly journals Flagging performance of two automated hematology analyzers in blast cell screening

2021 ◽  
Vol 9 (20) ◽  
pp. 1606-1606
Author(s):  
Yang Zhang ◽  
Pingli Yu ◽  
Zhixin Chen ◽  
Jingling Zhang ◽  
Qiu Lin ◽  
...  
Keyword(s):  
Blast Cell ◽  
Hematology Analyzers

High Fibrinopeptide A (FPA) Levels in Acute Non-Lymphocytic Leukemia Are Reduced by Heparin Administration

1984 ◽  
Vol 52 (03) ◽  
pp. 301-304 ◽  
Author(s):  
L Gugliotta ◽  
Silvana Viganò ◽  
A D’Angelo ◽  
Anna Guarini ◽  
S Tura ◽  
...  
Keyword(s):  
Body Temperature ◽  
Blast Cell ◽  
Lymphocytic Leukemia ◽  
Healthy Controls ◽  
Intravenous Bolus ◽  
Fibrinopeptide A ◽  
Cell Counts ◽  
Heparin Administration ◽  
Blast Cells ◽  
Baseline Values

SummaryPlasma levels of fibrinopeptide A (FPA) in 30 untreated patients with acute non-lymphocytic leukemia (ANLL) were significantly higher than in 30 healthy controls (p <0.001). Patients without laboratory signs of disseminated intravascular coagulation (DIC) had levels of FPA higher than controls (p <0.02) but markedly lower than patients with DIC (p <0.001). Five patients with M3 leukemia had a higher mean FPA level (p <0.02) and a lower peripheral blast cell count (p <0.05) than patients with other cytological subtypes of ANLL. When patients with M3 were excluded, a significant correlation was observed between the peripheral blast cell counts and the FPA levels (r = 0.66, p <0.001). FPA levels were similar with body temperature either above or below 38° C. After intravenous bolus of heparin FPA dropped to normal levels in 14 out of 17 patients who had high baseline values. These findings indicate that intravascular thrombin formation, which probably result from the expression of procoagulant activities of blast cells, is the main cause of high FPA in the majority of patients with acute non-lymphocytic leukemia.

scholarly journals Rapid prediction of stem cell mobilization using volume and conductivity data from automated hematology analyzers

Transfusion ◽  
2017 ◽  
Vol 58 (2) ◽  
pp. 330-338
Author(s):  
Carlos H. Villa ◽  
Thomas Porturas ◽  
Mary Sell ◽  
Mark Wall ◽  
Gene DeLeo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Mobilization ◽  
Conductivity Data ◽  
Rapid Prediction ◽  
Cell Mobilization ◽  
Hematology Analyzers

Signaling through interleukin-6 receptor supports blast cell proliferation in acute myeloblastic leukemia

2009 ◽  
Vol 61 (3) ◽  
pp. 190-196 ◽  
Author(s):  
Marjaana Säily ◽  
Pirjo Koistinen ◽  
Aiping Zheng ◽  
Eeva-Riitta Savolainen
Keyword(s):  
Cell Proliferation ◽  
Interleukin 6 ◽  
Blast Cell ◽  
Acute Myeloblastic Leukemia ◽  
Interleukin 6 Receptor

scholarly journals Establishment of improved review criteria for hematology analyzers in cancer hospitals

2020 ◽  
Author(s):  
Xiaoyan Wang ◽  
Xu Wang ◽  
Peng Ge ◽  
Xiaoqiang Zhao ◽  
Chong Chen ◽  
...  
Keyword(s):  
Review Criteria ◽  
Hematology Analyzers

Quality Control of Multichannel Hematology Analyzers: Bivariate On-line Comparison of MCV and MCH Values for the Detection of Random Errors

1992 ◽  
Vol 97 (5) ◽  
pp. 645-651
Author(s):  
Veli Kairisto ◽  
Timo Kouri ◽  
Allan Rajamäki ◽  
Arja Virtanen ◽  
Esa Uusipaikka ◽  
...  
Keyword(s):  
Quality Control ◽  
Random Errors ◽  
On Line ◽  
Hematology Analyzers

Role of Matrix Metalloproteinase MMP-2, MMP-9 and Tissue Inhibitor of Metalloproteinase (TIMP-1) in Clinical Progression of Pediatric Acute Lymphoblastic Leukemia

2021 ◽  
Author(s):  
Maha Saleh ◽  
Mohamed Khalil ◽  
Mona S. Abdellateif ◽  
Emad Ebeid ◽  
Eman Z. Kandeel
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Cell Count ◽  
Cancer Progression ◽  
Lymphoblastic Leukemia ◽  
Multivariate Logistic Regression Analysis ◽  
Blast Cell ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Tissue Inhibitor ◽  
Pediatric Acute Lymphoblastic Leukemia ◽  
Pediatric All

Abstract Background: Matrix metalloproteinases (MMPs) play a crucial role in cancer progression and metastasis, however their role in pediatric Acute lymphoblastic leukemia (ALL) is still unrevealed.Methods: The diagnostic, prognostic and predictive value of tissue inhibitor of metalloproteinase (TIMP-1), MMP-2, MMP-9 and CD34+CD38- CSCs were assessed in bone marrow (BM) samples of 76 ALL children using Flow Cytometry analysis. Results: There was a significant increase in TIMP-1 [1.52 (0.41-10) versus 0.91(0.6-1.12); respectively, P<0.001], and CSCs CD84+CD38- [1 (0.03-18.6) versus 0.3 (0.01-1.1), P<0.001] expression in ALL patients compared to controls. While there were no significant differences regarding MMP-2 and MMP-9 expression between the two groups. The sensitivity, specificity, AUC of MMP-2 were (80.3%, 53.3% and 0.568, P=0.404), and that of MMP-9 were (53.9%, 40% and 0.660, P=0.053). While that of TIMP-1 were (78.9%, 100% and 0.892, P<0.001), and that of CSCs CD34+ CD38- were (78.9%, 73.3% and 0.855, P<0.001). There was a significant association between MMP-2 overexpression and MRD at day-15, increased BM blast cell count at diagnosis and at day-15, (P=0.020, P=0.047 and P=0.001). Increased TIMP-1 expression associated with the high-risk disease (P<0.001), increased BM blast cell count at diagnosis and at day-15 (P=0.033 and P=0.001), as well as MRD at day 15 and day 42 (P<0.001 for both). CD34+CD38- CSCs associated with MRD at day-15, increased BM blast cell count at diagnosis and at day-15 (P=0.015, P=0.005 and P=0.003). TIMP-1 overexpression associated with shorter DFS and OS rates (P=0.009 and P=0.048). Multivariate logistic regression analysis showed that both TIMP-1 [OR: 4.224, P=0.046], and CD34+CD38- CSCs [OR: 6.873, P=0.005] are independent diagnostic factors for pediatric ALL.Conclusion: TIMP-1 and CD34+CD38- CSCs could be useful independent diagnostic markers for pediatric ALL. Also, TIMP-1 is a promising prognostic marker for poor outcome of the patients.

scholarly journals Effect of Phytohemagglutinin on Enzymes of Thymidine Salvage Pathway of Cultured Chronic Lymphatic Leukemic Lymphocytes

Blood ◽  
1970 ◽  
Vol 35 (2) ◽  
pp. 236-242 ◽  
Author(s):  
YALE RABINOWITZ ◽  
POLLY WONG ◽  
BETTY A. WILHITE
Keyword(s):  
Cell Culture ◽  
Dna Polymerase ◽  
Enzyme Activities ◽  
Kinase Activity ◽  
Cell Formation ◽  
Polymerase Activity ◽  
Blast Cell ◽  
Salvage Pathway ◽  
Culture Cells ◽  
Cells Cultured

Abstract N-L and CLL-L prior to cell culture gave evidence of high activities of TdR phosphorylase, TMP and TDP kinases, while TdR kinase and phosphatase activities were relatively low. Cells cultured without PHA showed no appreciable alteration in enzyme activities during three to five days of culture. Response to PHA with blast-cell formation in cultures of N-L or CLL-L (low-count cases) was associated with increased TdR kinase activity which became prominent at the time of increased DNA polymerase activity and DNA synthesis during the second to third day of culture. Cells from cases of CLL-L with high WBC counts which failed to respond to PHA showed no increase in TdR kinase activity but did show increased phosphatase activity. Increased TdR activity and increased DNA polymerase activity may both be needed for increased synthesis of DNA to occur in lymphocytes responding to PHA.

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