scholarly journals Reaction of APUD-System Cells of Experimental Biomodels to Subcutaneous Immunization by Yersinia pestis EV Vaccine Strain

2008 ◽  
pp. 46-49
Author(s):  
S. A. Bugorkova ◽  
S. Yu. Zadumina ◽  
V. V. Kutyrev
Keyword(s):  
Yersinia Pestis ◽  
Vaccine Strain ◽  
Subcutaneous Immunization ◽  
Apud System

scholarly journals Selective Protective Potency of Yersinia pestis ΔnlpD Mutants

Acta Naturae ◽  
2015 ◽  
Vol 7 (1) ◽  
pp. 102-108 ◽  
Author(s):  
S. V. Dentovskaya ◽  
S. A. Ivanov ◽  
P. Kh. Kopylov ◽  
R. Z. Shaikhutdinova ◽  
М. E. Platonov ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Vaccine Strain ◽  
Guinea Pigs ◽  
Subcutaneous Administration ◽  
Comparative Testing ◽  
Pneumonic Plague ◽  
The One ◽  
Potent Protection ◽  
Better Than

It has recently been shown that the NlpD lipoprotein is essential to Yersinia pestis virulence and that subcutaneous administration of the nlpD mutant could protect mice against bubonic and pneumonic plague better than the EV vaccine strain [PLoS One 2009. V. 4. № 9. e7023]. In this study, similar nlpD mutants were generated on the basis of other Y. pestis parent strains, including strains from the subspecies microtus, which is avirulent to guinea pigs and humans. Comparative testing confirmed that immunization of mice with nlpD mutants induces immunity 105 times more potent than the one induced by the administration of the EV vaccine strain. At the same time, NlpD- bacteria failed to protect guinea pigs in the case of a subcutaneous challenge with Y. pestis, inducing a 106 times less potent protection compared with that conferred by immunization with the EV vaccine strain. The possible causes of the observed phenomena are discussed.

scholarly journals Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG

2021 ◽  
pp. 70-74
Author(s):  
A. L. Kravtsov ◽  
A. Yu. Goncharova ◽  
S. A. Bugorkova ◽  
Z. L. Devdariani ◽  
V. A. Kozhevnikov
Keyword(s):  
Yersinia Pestis ◽  
Vaccine Strain ◽  
Virulent Strain ◽  
Abdominal Cavity ◽  
Fluorescent Microscopy ◽  
Neutrophil Extracellular Traps ◽  
The Body ◽  
Neutrophil Granulocytes ◽  
Extracellular Traps

The purpose of the study was to determine the effect of Yersinia pestis EV NIIEG on the process of neutrophil extracellular traps formation in vivo when modeling plague infection and assess their contribution to antiplague protection.Materials and methods. BALB/c mice, which were immunized subcutaneously with the Y. pestis EV NIIEG vaccine strain, were used in the study. Animals were infected with a virulent strain Y. pestis 231 at a dose of 20 LD50 (103 CFU). To evaluate the contribution of neutrophil extracellular traps (NETs) to antibacterial protection, an experimental model was used based on fermenting NETs in the abdominal cavity of mice with nuclease. To calculate the number of NETs in peritoneal exudate (PE) fluorescent microscopy was applied. Phagocytic activity of PE cells was determined by flow cytometry. Bactericidal effect of NETs was recorded using bacteriological method.Results and discussion. In pre-immunized mice, the process of NETs formation in response to the reintroduction of plague microbe living cells was 5 times more intense than in intact animals and was accompanied by a significant increase in the killing of Y. pestis cells in PE. The use of micrococcus nuclease in the experiment for fermentation of the NETs, produced in the body of immunized animals, provided evidence of NET participation in conferring anti-infective protection against plague infection. Thus, the established fact of the NET formation in case of Y. pestis infection of mice immunized with Y. pestis EV NIIEG vaccine strain and the influence of this process on the effectiveness of protection against plague is the basis for further clarifying the immunopathogenetic role of neutrophil granulocytes in plague. 

scholarly journals Experimental Evaluation of Application of the Vaccine Strain Yersinia pestis EV NIIEG in Combination with Immune-Modulators

2020 ◽  
pp. 71-77
Author(s):  
A. Yu. Goncharova ◽  
S. A. Bugorkova ◽  
O. M. Kudryavtseva ◽  
V. A. Kozhevnikov ◽  
A. L. Kravtsov ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Vaccine Strain ◽  
Cellular Response ◽  
Humoral Response ◽  
Average Life Expectancy ◽  
Histological Changes ◽  
Immune Modulators ◽  
Experimental Animals ◽  
Combined Use

Abstract. Objective of the work was to conduct a comparative assessment of the immune-modulating effect of the combined use of Yersinia pestis EV NIIEG vaccine strain with Polyoxidonium and Ingaron preparations on a BALB/c mouse model.Materials and methods. Mice of the BALB/c line were immunized subcutaneously with Yersinia pestis EV NIIEG culture at a dose of 2.5 104 m.c. (1st group), in combination with Ingaron at a dose of 150 IU (2nd group) or with Polyoxidonium at a dose of 4 мg (3rd group), the 4th group is intact mice. On days 3, 7, 21 and 90 after immunization, the subpopulation composition of lymphocytes, the production of mediators of the cellular response (INF-ɣ and IL-10), the titers of specific antibodies to the capsular antigen of plague microbe (F1), the nuclear apparatus of lymphocytes, and the nature of histological changes in the organs of mice were determined. Characterization of immunogenic (protective) activity of the combined use of Y. pestis EV NIIEG with immune-modulators against Y. pestis 231 in experiments on BALB/c mice was performed on the 21st day after immunization through determining the number of dead animals and their average life expectancy.Results and discussion. The combined administration of Y. pestis EV NIIEG vaccine strain with Polyoxidonium or Ingaron to experimental animals allowed us to establish differences in the response of the immune system of biomodels, due to the mechanism of action of a specific immune-modulator. It has been established that both Polyoxidonium and Ingaron combined with Y. pestis EV NIIEG enhance the response of immune-competent cells in experimental animals, contribute to the activation of the humoral response and the production of mediators of the cellular response, do not have a damaging effect on the tissue of the macroorganism. At the same time, the efficacy of using combined vaccination of Y. pestis EV NIIEG with immune-modulators in the inoculation test is confirmed for Polyoxidonium only.

scholarly journals An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection

2014 ◽  
Vol 21 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Juan Huang ◽  
Renyong Jia ◽  
Mingshu Wang ◽  
Bing Shu ◽  
Xia Yu ◽  
...  
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Vaccine Strain ◽  
Severe Disease ◽  
Harderian Gland ◽  
Bursa Of Fabricius ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mucosal Immune Responses ◽  
Subcutaneous Immunization ◽  
Main Routine

ABSTRACTDuck plague (DP) is a severe disease caused by DP virus (DPV). Control of the disease is recognized as one of the biggest challenges in avian medicine. Vaccination is an efficient way to control DPV, and an attenuated vaccine is the main routine vaccine. The attenuated DPV vaccine strain CHa is a modified live vaccine, but the systemic and mucosal immune responses induced by this vaccine have been poorly understood. In this study, the immunogenicity and efficacy of the vaccine were evaluated after subcutaneous immunization of ducks. CD4+and CD8+T cells were counted by flow cytometry, and humoral and mucosal Ig antibodies were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that high levels of T cells and Ig antibodies were present postimmunization and that there were more CD4+T cells than CD8+T cells. Titers of humoral IgG were higher than those of humoral IgA. Local IgA was found in each sample, whereas local IgG was found only in the spleen, thymus, bursa of Fabricius, harderian gland, liver, bile, and lung. In a protection assay, the attenuated DPV vaccine completely protected ducks against 1,000 50% lethal doses (LD50) of the lethal DPV strain CHv via oral infection. These data suggest that this subcutaneous vaccine elicits sufficient systemic and mucosal immune responses against lethal DPV challenge to be protective in ducks. This study provides broad insights into understanding the immune responses to the attenuated DPV vaccine strain CHa through subcutaneous immunization in ducks.

scholarly journals Effect of Bacteriophages and Gentamycine on Morphology and Vesicle Formation of Bacteria Yersinia pestis EV

2019 ◽  
pp. 50-54 ◽  
Author(s):  
L. G. Dudina ◽  
M. A. Malkova ◽  
A. V. Chernyad’ev ◽  
S. G. Litvinets ◽  
A. A. Byvalov
Keyword(s):  
Yersinia Pestis ◽  
Vaccine Strain ◽  
Stress Factors ◽  
Growth Media ◽  
Vesicle Formation ◽  
Microbial Culture ◽  
Bacterial Cells ◽  
Cultivation Conditions ◽  
Functional Changes ◽  
Transmission Electron

Objective was to assess the effect of specific bacteriophages and gentamycine on the morphological-functional properties of bacteria in the vaccine strain Yersinia pestis EV.Materials and methods. The vaccine strain Y. pestis EV, Pokrovskaya bacteriophage and the pseudotuberculous diagnostic bacteriophage were used for the study. The microbial culture was grown on solid and in liquid growth media at 27 °C for 20–24 h. The co-incubation of bacteria and bacteriophage or gentamycine was carried out at 27 °C for 20 minutes or at 37 °C for 2 hours, respectively. Culture preparations were examined by transmission electron microscopy.Results and discussion. The influence of cultivation conditions and various stress factors on the vesicle production by the vaccine strain Y. pestis EV cells was evaluated. The nature and intensity of morphological-functional changes in Y. pestis EV cells in response to the effect of bacteriophages (plague Pokrovskaya and pseudotuberculous bacteriophages) or an antibiotic (gentamycine) were determined. It was established that co-incubation of Y. pestis EV with Pokrovskaya bacteriophage or gentamycine for 20 min leads to the increase in the production of extracellular vesicles and is accompanied by the development of degenerative changes in bacterial cells. 

scholarly journals Qualitative Indicators of Microbial Cells of Yersinia pestis EV Strain Depending on Their Morphological Traits in Different Temperature Conditions of Manufacturing of Plague Vaccine Preparation

2012 ◽  
pp. 33-35
Author(s):  
D. A. Budyka ◽  
A. I. Bondarenko ◽  
A. A. Fisun ◽  
N. V. Abzaeva ◽  
G. F. Ivanova ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Biomass Production ◽  
Vaccine Strain ◽  
Morphological Traits ◽  
Small Cells ◽  
Microbial Cells ◽  
Vaccine Preparation ◽  
Temperature Conditions ◽  
Original Interpretation ◽  
Plague Vaccine

The preference of the (21±1) °C temperature for cultivation of the vaccine strain Yersinia pestis EV in the live plague vaccine biomass production to (27±1) °C is experimentally confirmed. An original interpretation of the mechanism of small cells stability that are formed at (21±1) °C has been proposed. This interpretation stipulates a smaller damaging effect of steam molecules during sublimation in the process of lyophilization.

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