scholarly journals IDENTIFICATION AND MAPPING OF A GENE FOR RICE SLENDER KERNEL USING Oryza glumaepatula INTROGRESSION LINES

2016 ◽  
Vol 9 (2) ◽  
pp. 55
Author(s):  
Sobrizal Sobrizal ◽  
Atsushi Yoshimura
Keyword(s):  
Grain Shape ◽  
Recessive Gene ◽  
Introgression Lines ◽  
Rflp Markers ◽  
Recurrent Parent ◽  
Rice Grain ◽  
Chromosome 2 ◽  
Plant Materials ◽  
Bc4f2 Population ◽  
Oryza Glumaepatula

<br />World demand for superior rice grain quality tends to increase. One of the  criteria of appearance quality of rice grain is grain shape. Rice consumers  exhibit wide preferences for grain shape, but most Indonesian rice consumers prefer long and slender grain. The objectives of this study were to identify and map a gene for rice slender kernel trait using Oryza  glumaepatula introgression lines with O. sativa cv. Taichung 65 genetic background. A segregation analysis of BC4F2 population derived from backcrosses of a donor parent O. glumaepatula into a recurrent parent Taichung 65 showed that the slender kernel was controlled by a single recessive gene. This new identified gene was designated as sk1 (slender kernel 1). Moreover, based on the RFLP analyses using 14 RFLP markers located on chromosomes 2, 8, 9, and 10 in which the O. glumaepatula chromosomal segments were retained in BC4F2 population, the sk1 was located between RFLP markers C679 and C560 on the long arm of chromosome 2, with map distances of 2.8 and 1.5 cM, respectively. The wild rice O. glumaepatula carried a recessive allele for slender kernel. This allele may be useful in breeding of rice with slender kernel types. In addition, the development of plant materials and RFLP map associated with slender kernel in this study is the preliminary works in the effort to isolate this important grain shape gene.

scholarly journals IDENTIFICATION AND MAPPING OF A GENE FOR RICE SLENDER KERNEL USING Oryza glumaepatula INTROGRESSION LINES

2016 ◽  
Vol 9 (2) ◽  
pp. 55
Author(s):  
Sobrizal Sobrizal ◽  
Atsushi Yoshimura
Keyword(s):  
Grain Shape ◽  
Recessive Gene ◽  
Introgression Lines ◽  
Rflp Markers ◽  
Recurrent Parent ◽  
Rice Grain ◽  
Chromosome 2 ◽  
Plant Materials ◽  
Bc4f2 Population ◽  
Oryza Glumaepatula

<br />World demand for superior rice grain quality tends to increase. One of the  criteria of appearance quality of rice grain is grain shape. Rice consumers  exhibit wide preferences for grain shape, but most Indonesian rice consumers prefer long and slender grain. The objectives of this study were to identify and map a gene for rice slender kernel trait using Oryza  glumaepatula introgression lines with O. sativa cv. Taichung 65 genetic background. A segregation analysis of BC4F2 population derived from backcrosses of a donor parent O. glumaepatula into a recurrent parent Taichung 65 showed that the slender kernel was controlled by a single recessive gene. This new identified gene was designated as sk1 (slender kernel 1). Moreover, based on the RFLP analyses using 14 RFLP markers located on chromosomes 2, 8, 9, and 10 in which the O. glumaepatula chromosomal segments were retained in BC4F2 population, the sk1 was located between RFLP markers C679 and C560 on the long arm of chromosome 2, with map distances of 2.8 and 1.5 cM, respectively. The wild rice O. glumaepatula carried a recessive allele for slender kernel. This allele may be useful in breeding of rice with slender kernel types. In addition, the development of plant materials and RFLP map associated with slender kernel in this study is the preliminary works in the effort to isolate this important grain shape gene.

scholarly journals Substitution Mapping of Two Closely Linked QTLs Controlling Grain Chalkiness and Grain Shape on Rice Chromosome 8

2021 ◽  
Author(s):  
Weifeng Yang ◽  
Liang Xiong ◽  
Jiayan Liang ◽  
Qingwen Hao ◽  
Xin Luan ◽  
...  
Keyword(s):  
Grain Quality ◽  
Grain Shape ◽  
Rice Chromosome ◽  
Chromosome 8 ◽  
High Yield ◽  
Rice Grain ◽  
Rice Varieties ◽  
Substitution Mapping ◽  
Grain Width ◽  
Grain Chalkiness

Abstract Background: Rice varieties are required to have high yield and good grain quality. Grain chalkiness and grain shape are two important traits of rice grain quality. Low chalkiness slender grains are preferred by most rice consumers. Here, we dissected two closely linked quantitative trait loci (QTLs) controlling grain chalkiness and grain shape on rice chromosome 8 by substitution mapping. Results: Two closely linked QTLs controlling grain chalkiness and grain shape were identified using single-segment substitution lines (SSSLs). The two QTLs were then dissected on rice chromosome 8 by secondary substitution mapping. qPGC8.1 was located in an interval of 1382.6 kb and qPGC8.2 was mapped in a 2057.1 kb region. The maximum distance of the two QTLs was 4.37 Mb and the space distance of two QTL intervals was 0.72 Mb. qPGC8.1 controlled grain chalkiness and grain width. qPGC8.2 was responsible for grain chalkiness and for grain length and grain width. The additive effects of qPGC8.1 and qPGC8.2 on grain chalkiness were not affected by heat stress. Conclusions: Two closely linked QTLs qPGC8.1 and qPGC8.2 were dissected on rice chromosome 8. They controlled the phenotypes of grain chalkiness and grain shape. The two QTLs were insensitive to high temperature.

scholarly journals Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross

2015 ◽  
Vol 65 (4) ◽  
pp. 308-318 ◽  
Author(s):  
Kazufumi Nagata ◽  
Tsuyu Ando ◽  
Yasunori Nonoue ◽  
Tatsumi Mizubayashi ◽  
Noriyuki Kitazawa ◽  
...  
Keyword(s):  
Genetic Control ◽  
Qtl Analysis ◽  
Grain Shape ◽  
Rice Grain ◽  
Advanced Backcross

scholarly journals Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9–1

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Liangrong Jiang ◽  
Guotian Li ◽  
Mawsheng Chern ◽  
Rashmi Jain ◽  
Nhan T. Pham ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Grain Shape ◽  
Rice Grain ◽  
Whole Genome

Novel OsGRAS19 mutant, D26, positively regulates grain shape in rice (Oryza sativa)

2019 ◽  
Vol 46 (9) ◽  
pp. 857 ◽  
Author(s):  
Zhimin Lin ◽  
Jingwan Yan ◽  
Jun Su ◽  
Huaqing Liu ◽  
Changquan Hu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Grain Size ◽  
Transcription Factor ◽  
Grain Shape ◽  
Rice Yield ◽  
Reproductive Development ◽  
Cell Number ◽  
Rice Grain ◽  
Regulatory Effect ◽  
Site Mutation

Grain size is an important factor in rice yield. Several genes related to grain size have been reported, but most of them are determined by quantitative trail loci (QTL) traits. Gene D26 is a novel site mutation of OsGRAS19 and involved in the brassinosteroid (BR) signalling pathway. However, whether D26 is involved in the process of rice reproductive development remains unclear. Here, gene cloning and functional analysis revealed that D26 has an obvious regulatory effect on grain size. Overexpression or CRISP/Cas9 mutant of D26 also showed that grain size was positively influenced. Cellular analyses show that D26 modulates grain size by promoting cell division and regulating the cell number in the upper epidermis of the glume. The overexpression results further suggest that the level of D26 expression positively impacts grain length and leaf angles and that the expression of several known grain size genes is involved in the regulation. Based on our results, D26, as a transcription factor, effectively improves rice grain shape.

scholarly journals Combining two semidwarfing genes d60 and sd1 for reduced height in ‘Minihikari’, a new rice germplasm in the ‘Koshihikari’ genetic background

2012 ◽  
Vol 94 (5) ◽  
pp. 235-244 ◽  
Author(s):  
MOTONORI TOMITA
Keyword(s):  
Recessive Gene ◽  
Segregation Ratio ◽  
Pollen Sterility ◽  
Recurrent Parent ◽  
Lethal Gene ◽  
Mendelian Segregation ◽  
Dwarf Gene ◽  
Reduced Height ◽  
Rice Yields ◽  
Semidwarf Gene

SummaryDwarfing in rice has dramatically improved and stabilized rice yields worldwide, often controlled by a single dwarf gene, sd1. A novel semidwarf gene d60 complements the gametic lethal gene gal, such that the F1 between ‘Hokuriku 100’ (genotype d60d60GalGal, Gal: mutant non-lethal allele) and ‘Koshihikari’ (D60D60galgal, D60: tall allele) would show 25% sterility due to deterioration of gametes bearing both gal and d60. The F2 would segregate as one semidwarf (1 d60d60GalGal) : two tall and 25% sterile (2 D60d60Galgal) : six tall (2 D60d60GalGal : 1 D60D60GalGal : 2 D60D60Galgal : 1 D60D60galgal), skewed from a Mendelian segregation ratio of one semidwarf : three tall for a single recessive gene. To pyramid d60 and sd1, into the Japanese super-variety ‘Koshihikari’, the F1 (D60d60Galgal) of ‘Koshihikari’ × ‘Hokuriku 100’ was first backcrossed with ‘Koshihikari’, and the BCF1 segregated into a ratio of one tall and 25% sterile (D60d60Galgal) : two tall (1 D60D60Galgal : 1 D60D60galgal). Tall, 25% sterile BC1F1 plants (D60d60Galgal) were then selected for pollen sterility and backcrossed with ‘Koshihikari’ as the recurrent parent. It is unnecessary to grow out and select a semidwarf from the BCnF2 if a pollen parent with ∼70% pollen fertility is chosen from the BCnF1 to backcross with the recurrent parent. Semidwarfing genes d60 and sd1 were successfully pyramided into the ‘Koshihikari’ genome by crossing isogenic lines ‘Koshihikari d60’ and ‘Koshihikari sd1’, to produce ‘Minihikari’, a new parental source of both d60 and sd1. ‘Minihikari’ displayed super-short stature due to the combination of sd1 and d60, which are genetically and functionally independent.

Genetic bases of rice grain shape: so many genes, so little known

2013 ◽  
Vol 18 (4) ◽  
pp. 218-226 ◽  
Author(s):  
Rongyu Huang ◽  
Liangrong Jiang ◽  
Jingsheng Zheng ◽  
Tiansheng Wang ◽  
Houcong Wang ◽  
...  
Keyword(s):  
Grain Shape ◽  
Rice Grain ◽  
Genetic Bases

scholarly journals GW10, a Member of P450 Subfamily Regulates Grain Size and Grain Number in Rice

2021 ◽  
Author(s):  
PengLin Zhan ◽  
Xin Wei ◽  
Zhili Xiao ◽  
Xiaoling Wang ◽  
Shuaipeng Ma ◽  
...  
Keyword(s):  
Grain Size ◽  
Grain Yield ◽  
Grain Shape ◽  
Rice Grain ◽  
Chromosome 10 ◽  
Grain Number ◽  
Tropical Japonica ◽  
Donor Parent ◽  
Lower Expression ◽  
Recipient Parent

Abstract Grain size and grain number play extremely important roles in rice grain yield. Here, we identify GW10 , which encodes a P450 subfamily protein and controlls grain size and grain number by using Lemont ( tropical japonica ) as donor parent and HJX74 ( indica ) as recipient parent. The GW10 locus was mapped into a 20.1 kb region on the long arm of Chromosome 10. Lower expression of the gw10 in panicle is contributed to the shorter and narrower rice grain, and the increased number of grains per panicle. Furthermore, the higher expression levels of some of the brassinosteroid (BR) biosynthesis and response genes are associated with the NIL- GW10 , which strongly suggests that the GW10 is a key node in the brassinosteroid-mediated regulation of rice grain shape and grain number.

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