EFISIENSI MEDIA KULTUR DAN APLIKASI TEMPORARY IMMERSION SYSTEM PADA EMBRIOGENESIS SOMATIK KOPI ARABIKA / Efficiency of Culture Media and  Aplication Temporary Immersion Systemon on Somatic Embryogenesis Arabica Coffee

Título en imgles: Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.) Título corto: Un avance para la micropropagación comercial de zábilaResumen: La micropropagación es una alternativa para la producción comercial de plantas de zábila (Aloe barbadensis Mill.) limitada por los altos costos de producción. Con el objetivo de prescindir de los agentes gelificantes, reduciendo costos, se comparó el medio de cultivo líquido con el medio de cultivo gelificado en las diferentes etapas de micropropagación de la zábila. En la etapa de establecimiento se observó mayor porcentaje de explantes contaminados en el medio de cultivo líquido estático (25.55%) que en el medio gelificado (11.11%); y aunque el resto de los explantes se establecieron independientemente de la condición del medio de cultivo, en el medio líquido alcanzaron mayor altura (3.81 cm) que en el medio gelificado (3.03 cm). En la etapa de multiplicación, la altura de los explantes (entre 4.43 y 6.01 cm) fue superior en los recipientes de inmersión temporal automatizado (RITA®) en comparación con el medio gelificado (entre 3.24 y 3.42 cm); sin diferencias significativas entre el número de brotes/explante. Todos los brotes enraizaron a los 30 días independientemente del medio de cultivo empleado (líquido estático y gelificado), sin observar variaciones en la altura del brote y, número y longitud de las raíces. El empleo de los medios de cultivo líquidos y la implementación de los sistemas de inmersión temporal tipo RITA® permiten reducir los costos de producción al prescindir de los agentes gelificantes, lo que representa un avance para la micropropagación comercial de zábila. Palabras clave: Cultivo de tejidos, agentes gelificantes, RITA®, sistemas de inmersión temporal.Abstract: Micropropagation is considered a successful alternative for aloe (Aloe barbadensis Mill.) plant production. However, it has limited use due to the high production cost. Liquid media were compared to agar-gelled medium during all micropropagation stages of aloe to reduce the cost for gelling agent used. In the establishment stage, there was a higher percentage of contaminated explants in static liquid medium (25.55%) than those cultured in agar-gelled medium (11.11%), although all the explants were established independently of the culture medium used, higher height (3.81 cm) was observed in liquid medium than those growing in agar-gelled medium (3.03 cm). In the multiplication stage, explant height was higher in the recipients used for automated temporary immersion system (RITA®) (4.43‑6.01 cm) than those cultured in agar-gelled medium (3.24‑3.42 cm), there was no significant difference for number of shoots/explant. All shoots had roots at 30 days independently of used culture media (static liquid or agar-gelled media). Shoot height, number and root length had similar values in both culture media. The implementation of liquid media and automated temporary Immersion system RITA® may allow to reduce production costs of gelling agent used, it represents an approach for the commercial micropropagation of aloe.Keys words: Tissue culture, gelling agents, RITA®, temporary immersion system.Recibido: junio 15 de 2014 Aprobado: abril 13 de 2015

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Plant regeneration via somatic embryogenesis of Camptotheca acuminata in temporary immersion system (TIS)

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-008-9428-3 ◽

2008 ◽

Vol 95 (2) ◽

pp. 163-173 ◽

Cited By ~ 16

Author(s):

Yantree Devi Sankar-Thomas ◽

Katja Saare-Surminski ◽

Reinhard Lieberei

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Camptotheca Acuminata ◽

Temporary Immersion System ◽

Temporary Immersion

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Improved high-efficiency protocol for somatic embryogenesis in Peach Palm (Bactris gasipaes Kunth) using RITA® temporary immersion system

Scientia Horticulturae ◽

10.1016/j.scienta.2014.09.041 ◽

2014 ◽

Vol 179 ◽

pp. 284-292 ◽

Cited By ~ 16

Author(s):

Angelo S. Heringer ◽

Douglas A. Steinmacher ◽

Hugo P.F. Fraga ◽

Leila N. Vieira ◽

Tiago Montagna ◽

...

Keyword(s):

Somatic Embryogenesis ◽

High Efficiency ◽

Bactris Gasipaes ◽

Temporary Immersion System ◽

Temporary Immersion ◽

Peach Palm

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Peach palm plantlet growth in different culture media in a temporary immersion system

Ciência Rural ◽

10.1590/0103-8478cr20190075 ◽

2021 ◽

Vol 51 (3) ◽

Author(s):

João Henrique Delfrate Padilha ◽

Douglas Steinmacher ◽

Marguerite Quoirin

Keyword(s):

Culture Media ◽

Growth Period ◽

Ms Medium ◽

High Concentration ◽

Temporary Immersion System ◽

Temporary Immersion ◽

Peach Palm ◽

Plantlet Growth ◽

Commercially Important ◽

N6 Medium

ABSTRACT: Peach palm is a domesticated palm commercially important for the production of fruits and hearts of palm. Somatic embryogenesis, an effective technique for mass propagation, was successfully established for this species. Furthermore, a temporary immersion system improved plant regeneration. However, production can be further improved by understanding the peach palm’s growth dynamic and modifications of culture media. The aims of this study were to evaluate the growth of plantlets cultured in different culture media in a temporary immersion system and to correlate the results with nutrient uptake during the growth period. Somatic embryo-derived young plantlets approximately 1 cm in length were cultivated for 12 weeks in a twin flask system containing MS, Y3 or N6 salts, Morel and Wetmore vitamins and 3% sucrose, with a monthly medium refreshment. Growth was measured and mineral analysis of the plantlets was carried out after 12 weeks of culture. The Y3 and MS salts were the most appropriate for the plant growth. Number of roots was 52.52% higher and the root size was 40.42% between the N6 and MS medium and the root number in Y3 medium was 37.74% greater than in MS medium, which is important for post acclimatization survival. K and Na are important elements for peach palm. N is not required at such a high concentration as in Murashige and Skoog formulation. The Chu (N6) medium did not generate high quality plantlets, possibly due to the absence of some micronutrients, like Mo, Cu and Co.

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In Vitro Culture of Rosmarinus officinalis L. in a Temporary Immersion System: Influence of Two Phytohormones on Plant Growth and Carnosol Production

Pharmaceuticals ◽

10.3390/ph14080747 ◽

2021 ◽

Vol 14 (8) ◽

pp. 747

Author(s):

Eder Villegas Sánchez ◽

Mariana Macías-Alonso ◽

Soraya Osegueda Robles ◽

Lisset Herrera-Isidrón ◽

Hector Nuñez-Palenius ◽

...

Keyword(s):

High Performance ◽

Emerging Infectious Diseases ◽

Rosmarinus Officinalis ◽

Wild Plant ◽

Array Detector ◽

Phase System ◽

Naphthaleneacetic Acid ◽

Temporary Immersion System ◽

Temporary Immersion

Emerging infectious diseases have become a major global problem with public health and economic consequences. It is an urgent need to develop new anti-infective therapies. The natural diterpene carnosol exhibit a wide variety of interesting antibacterial and antiviral properties, and it is considered a theoretical inhibitor of COVID-19 Mpro. However, this compound is present in the family Lamiaceae in low quantities. To obtain carnosol in concentrations high enough to develop pharmacological studies, we evaluated the efficiency of a micropropagation protocol of Rosmarinus officinalis using a solid medium and a temporary immersion system (TIS), as well as the effect of 6-benzylaminopurine (6-BAP) and α-naphthaleneacetic acid (NAA) on the growth of shoots. Moreover, we developed and validated an analytical method to quantify carnosol using the H-point standard additions method in the high-performance liquid chromatography diode array detector (HPLC-DAD). After 30 days of culture, TIS produced the maximum number of shoots per explant (24.33 ± 1.15) on a liquid medium supplemented with 6-BAP at 5.0 mg L−1. Next, we also evaluated the effect of immersion time and frequency for TIS. After 72 days of culture, the best results were obtained with an immersion cycle of 1 min every 12 h, yielding 170.33 ± 29.40 shoots. The quantification of carnosol on the samples was performed at a flow rate of 1.2 mL min−1 using binary isocratic mobile phase system 60:40 (v/v) 10 mM formic acid (pH 3.0) (A) and acetonitrile (B) on a reverse-phase column. The content of carnosol in the in vitro cultures was around 8-fold higher than in the wild plant. The present study represents an efficient alternative method to obtain carnosol for its pre-clinical and clinical development.

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The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v27i2.146 ◽

2011 ◽

Vol 27 (2) ◽

Author(s):

Rina Arimarsetiowati

Keyword(s):

Tissue Culture ◽

Somatic Embryogenesis ◽

Coffea Arabica ◽

Callus Formation ◽

Culture Technique ◽

Plant Production ◽

Direct Somatic Embryogenesis ◽

Arabica Coffee ◽

Randomized Design ◽

Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

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