scholarly journals Study of some effective factors on the production of garamicidin from locally isolated Bacillus brevis

2007 ◽  
Vol 4 (2) ◽  
pp. 215-220
Author(s):  
Baghdad Science Journal
Keyword(s):  
Carbon Source ◽  
Organic Solvent ◽  
Nitrogen Source ◽  
Ammonium Chloride ◽  
Bacillus Brevis ◽  
Effective Factors ◽  
Local Isolate ◽  
Cultural Conditions ◽  
Phosphate Source

The optimum cultural conditions for garamicidin production by local isolate B.brevis were studied.Best result was obtained when the isolate B.brevis was grown on media composed of 1%glucose as carbon source,1% ammonium chloride as a nitrogen source ,0.5% Dipotassium hydrogen orthophosphate as a phosphate source and after 48 hours of incubation at 30C .Garamicidin has been extracted and purified through acid precipition and then extracted by organic solvent (ether& acetone ).Using HPLC the garamicidin antibiotic showed three types A,B and C garamicidin .

Cell growth and pigment production in suspension cultures of a mycobiont isolated from the lichen Cladonia cristaiella

1995 ◽  
Vol 73 (S1) ◽  
pp. 590-594 ◽  
Author(s):  
Y. Yamamoto ◽  
Y. Kinoshita ◽  
T. Kurokawa ◽  
I. Yoshimura ◽  
V. Ahmadjian ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Liquid Culture ◽  
Pigment Production ◽  
Malt Extract ◽  
Nutritional Factors ◽  
Cultural Conditions ◽  
Acetone Extracts ◽  
Affect Cell Growth

This is the first study on the factors that affect cell growth and the production of secondary metabolites of a lichen mycobiont in liquid culture. An ascospore-derived strain of Cladonia cristatella mycobiont accumulated and excreted red pigments into a liquid medium. Growth of the mycobiont was increased by using liquid Lilly–Barnett medium containing 16% (w/v) sucrose as a carbon source, 0.2% (w/v) L-glutamine as a nitrogen source, and 0.2% (w/v) polypeptone, adjusting pH to 5.0 before autoclaving, and incubating cultures at 20 °C. Pigment production by the mycobiont was increased by using liquid Lilly–Barnett medium containing 4% (w/v) sucrose as a carbon source, 0.2% (w/v) L-asparagine as a nitrogen source, and 0.2% (w/v) malt extract, adjusting pH to 5.0 before autoclaving and incubating cultures at 20 °C. All acetone extracts under any cultural conditions yielded similar HPLC chromatograms. We proved no relationship between cell growth and secondary metabolism based on the nutritional factors in the cultured C. cristatella mycobiont. Key words: lichen, suspension culture, Cladonia cristatella mycobiont, red pigment, production, and growth factor.

scholarly journals Study on culture conditionsfor extracellular alginate lyase production by Bacillus velezensis AlgSm1

2021 ◽  
Vol 63 (3) ◽  
pp. 19-23
Author(s):  
Thi Thuy Hang Cao ◽  
◽  
Mai Nhu Hieu Vo ◽  
Duc Thinh Pham ◽  
Ngoc Linh Nguyen ◽  
...  
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
High Activity ◽  
Marine Bacteria ◽  
Ph Value ◽  
Alginate Lyase ◽  
Bacillus Velezensis ◽  
Cultural Conditions ◽  
Nutritional Components ◽  
Initial Ph

Alginate lyase is an enzyme that degrades alginate to create bioactive oligoalginate for application in medicine, industry, agriculture... In this study, cultural conditions of marine bacteria, Bacillus velezensis AlgSm1 have been investigated for producing alginate lyase with high content and activity. The nutritional components such as carbon source, nitrogen source, initial pH value, and cultural time were investigated. The results showed that the cultural conditions of B. velezensis AlgSm1 to biosynthesize alginate lyase with high activity were 5 mg/ml alginate, 0.8 mg/ml yeast extract; pH 5.5 and 18-hours fermentation at 28-30°C.

Studies on the Biological Characteristics of Termitomyces Albuminosus Hypha

2013 ◽  
Vol 709 ◽  
pp. 810-813 ◽  
Author(s):  
Xiong Ya ◽  
Min Jie Li
Keyword(s):  
Growth Factors ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Biological Characteristics ◽  
Edible Fungi ◽  
Growth Environment ◽  
Wild Edible Fungi ◽  
Development And Utilization

Termitomyces albuminosus is a kind of local distinctive wild edible fungi in southwest of China. It is delicious, rich in nutrition and has high development and utilization value, but owing to the restrictions of growth environment, it can not be cultivated artificially. This article mainly studied on the biological characteristics of Termitomyces albuminosus Hypha, and found out the optimal carbon source, nitrogen source, growth factors and the C/N ratio that are suitable for the growth of Hypha of Termitomyces albuminosus .

scholarly journals Monomethylamine as a Nitrogen Source for a Nonmethylotrophic Bacterium, Agrobacterium tumefaciens

2010 ◽  
Vol 76 (12) ◽  
pp. 4102-4104 ◽  
Author(s):  
Yin Chen ◽  
Kathryn L. McAleer ◽  
J. Colin Murrell
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Nitrogen Source ◽  
Key Enzymes ◽  
Genes Encoding

ABSTRACT Monomethylamine can be used by nonmethylotrophs as a sole nitrogen source but not as a carbon source; however, little is known about the genes and enzymes involved. The γ-glutamylmethylamide/N-methylglutamate pathway for monomethylamine utilization by methylotrophs has recently been resolved. We have identified genes encoding key enzymes of this pathway in nonmethylotrophs (e.g., Agrobacterium tumefaciens) and demonstrated that this pathway is also involved in the utilization of monomethylamine as a nitrogen source by nonmethylotrophs.

scholarly journals Mutants affecting histidine utilization inAspergillus nidulans

1975 ◽  
Vol 25 (2) ◽  
pp. 119-135 ◽  
Author(s):  
Meryl Polkinghorne ◽  
M. J. Hynes
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Carbon Source ◽  
Nitrogen Sources ◽  
Limiting Factor ◽  
Sole Nitrogen Source ◽  
Wild Type ◽  
Exogenous Substrate ◽  
Growth Properties ◽  
Type Strains

SUMMARYWild-type strains ofAspergillus nidulansgrow poorly onL-histidine as a sole nitrogen source. The synthesis of the enzyme histidase (EC. 4.3.1.3) appears to be a limiting factor in the growth of the wild type, as strains carrying the mutantareA102 allele have elevated histidase levels and grow strongly on histidine as a sole nitrogen source.L-Histidine is an extremely weak sole carbon source for all strains.Ammonium repression has an important role in the regulation of histidase synthesis and the relief of ammonium repression is dependent on the availability of a good carbon source. The level of histidase synthesis does not respond to the addition of exogenous substrate.Mutants carrying lesions in thesarA orsarB loci (suppressor ofareA102) have been isolated. The growth properties of these mutants on histidine as a sole nitrogen source correlate with the levels of histidase synthesized. Mutation at thesarA andsarB loci also reduces the utilization of a number of other nitrogen sources. The data suggest that these two genes may code for regulatory products involved in nitrogen catabolism. No histidase structural gene mutants were identified and possible explanations of this are discussed.

scholarly journals Production of Feruloyl Esterase from Aspergillus niger by Solid-State Fermentation on Different Carbon Sources

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Shiyi Ou ◽  
Jing Zhang ◽  
Yong Wang ◽  
Ning Zhang
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Wheat Bran ◽  
Cell Walls ◽  
Carbon Sources ◽  
Feruloyl Esterase ◽  
Plant Cell Walls ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Maize Bran

A mixture of wheat bran with maize bran as a carbon source and addition of (NH4)SO4 as nitrogen source was found to significantly increase production of feruloyl esterase (FAE) enzyme compared with wheat bran as a sole carbon and nitrogen source. The optimal conditions in conical flasks were carbon source (30 g) to water 1 : 1, maize bran to wheat bran 1 : 2, (NH4)SO4 1.2 g and MgSO4 70 mg. Under these conditions, FAE activity was 7.68 mU/g. The FAE activity on the mixed carbon sources showed, high activity against the plant cell walls contained in the cultures.

Ammoximation of cyclohexanone to cyclohexanone oxime using ammonium chloride as nitrogen source

2016 ◽  
Vol 152 ◽  
pp. 717-723 ◽  
Author(s):  
Yuanyuan Xu ◽  
Qiusheng Yang ◽  
Zhihui Li ◽  
Liya Gao ◽  
Dongsheng Zhang ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Ammonium Chloride ◽  
Cyclohexanone Oxime

scholarly journals KAJIAN PENGARUH PENINGKATAN KONSENTRASI EKSTRAK KHAMIR PADA MEDIA HESTRIN DAN SCHRAMM TERHADAP PEROLEHAN SELULOSA OLEH Acetobacter liquefaciens SRC-4

2014 ◽  
Vol 5 (2) ◽  
Author(s):  
Ermina Sari
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Vitamin B1 ◽  
Vitamin B2 ◽  
Static Culture

SRC-4 starin is one of bacteria sellulose producing strains. It was obtained from the rottencoconut meat from Semplak, Bogor. The objective of this study is to obtain the optimumcomposition of modified HS medium to produce cellulose, by increasing yeast extractconcentration and also combination of carbon and vitamin sources. The production of celluloseby SRC-4 was done by 18 treatments on static culture. The increasing of yeast extractconcentration, combination of carbon and vitamin sources gave significant effect to theproduction of cellulose. The highest yield of cellulose was produced at the treatment consist of1.0 % yeat extract (as nitrogen source), 1 % glucose + 1 % glycerol (as carbon source) and0.164 ppm vitamin B1 + 0.020 ppm vitamin B2 (as vitamin source).

Evaluation of the composition of continuously-cultivated Arthrospira (Spirulina) platensis using ammonium chloride as nitrogen source

2010 ◽  
Vol 34 (12) ◽  
pp. 1732-1738 ◽  
Author(s):  
C.E.N. Sassano ◽  
L.A. Gioielli ◽  
L.S. Ferreira ◽  
M.S. Rodrigues ◽  
S. Sato ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Ammonium Chloride ◽  
Spirulina Platensis
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