scholarly journals Early Pregnancy Diagnosis Using Milk Progesterone Kits in Etawah Cross Goat (Capra hircus)

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Milona Elsa Nova ◽  
Ginta Riady ◽  
Juli Melia
Keyword(s):  
Dairy Cows ◽  
Early Pregnancy ◽  
Capra Hircus ◽  
Estrous Female ◽  
Pregnancy Diagnosis ◽  
Negative Results ◽  
Diagnosis Tool ◽  
Natural Mating

This study aims to determine the effectiveness and accuracy of milk progesterone kit as an early pregnancy diagnosis tool for Etawah cross goat. This study used 9 lactating female goats with weight in the range of 35-51 kgs and the ages ranging from 2 to 2.5 years old. Eight goats were subject to estrous synchronisation protocol using progesterone implant (CIDR-G) followed by prostaglandin administration and one goat was left to show natural estrous. Early pregnancy diagnosis using milk progesterone kits was carried out on day 18 until 22 following services. The accuracy of the kits for early pregnancy diagnosis was subsequently confirmed using ultrasonography (USG) examination on day 35 following services. The results of this study showed that estrous response by synchronization was 50%. Whereas one female goat showed estrous sign naturally and was subject to pregnancy diagnosis procedure using the milk progesterone kits after natural mating. Pregnancy diagnosis using milk progesterone kits and subsequent confirmation using USG was carried out in five goats (4 synchronized females and mated and 1 naturally estrous female and mated naturally). All 5 female goats showed negative results following pregnancy diagnosis using the kits throughout the whole 5 days examinations. While diagnosis of pregnancy as confirmed by USG showed one goat (number 6) positive. It can be concluded that milk progesterone kits on dairy cows are less effective for diagnosing pregnancy on Etawah cross goat. The accuracy of the kits for pregnancy diagnosis of not-pregnant status is 80% after confirmation using USG.Key words: CIDR-G, Ettawah goats, milk progesterone kits, ultrasonography

Concentrations of progesterone in milk as a monitor of early pregnancy diagnosis in dairy cows

1979 ◽  
Vol 12 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Abdalla A. Zaied ◽  
C.J. Bierschwal ◽  
R.G. Elmore ◽  
R.S. Youngquist ◽  
A.J. Sharp ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Early Pregnancy ◽  
Pregnancy Diagnosis

116 EFFECTS OF EARLY PREGNANCY DIAGNOSIS USING ULTRASONOGRAPHY ON THE SUBSEQUENT EMBRYO AND FETAL LOSS IN DAIRY COWS

2008 ◽  
Vol 20 (1) ◽  
pp. 138
Author(s):  
I. H. Kim ◽  
J. I. Lee ◽  
U. H. Kim ◽  
H. G. Kang
Keyword(s):  
Dairy Cows ◽  
Incidence Rate ◽  
Early Pregnancy ◽  
Fetal Loss ◽  
Standard Group ◽  
Chi Square ◽  
Exact Test ◽  
Pregnancy Diagnosis ◽  
Array Transducer ◽  
National University

Under on-farm condition, pregnancy diagnosis can be rapidly and accurately diagnosed using ultrasound. However, the risk of the incidence of embryo and fetal loss following early pregnancy diagnosis has not been fully determined. This study assessed, using ultrasonography, the incidence of embryo and fetal loss in dairy cows following early pregnancy diagnosis after artificial insemination. A positive pregnancy was the recognition of the vesicle, embryo, or fetus by ultrasonography (Sonoace 600 with 5.0- or 7.5-MHz linear array transducer; Medison Co., Ltd., Seoul, Korea). Pregnancies (n = 702) were divided into three groups according to the number of days pregnant diagnosed: early A group (27 to 40 days, n = 143), early B group (41 to 50 days, n = 172), or standard group (51 to 70 days, n = 387). Following a positive pregnancy diagnosis, embryo or fetal loss included all cows with observed abortions and also cows found open after the positive pregnancy diagnosis. Data were analyzed by chi-square test or Fisher's exact test using an SAS program (SAS Institute, Inc., Cary, NC, USA). The incidence rate of embryo or fetal loss within 7 days after pregnancy diagnosis was 1.4, 0.6, and 0.3% for the early A, early B, and standard groups, respectively (P > 0.05). The incidence rate of the embryo or fetal loss during 8 to 30 days after pregnancy diagnosis was not different (P > 0.05) among the early A (0%), early B (1.2%), and standard groups (1.0%). Furthermore, the cumulative incidence rate of the embryo or fetal loss before calving was not different (P > 0.05) among the early A (9.8%), early B (9.3%), and standard groups (5.9%). In conclusion, early pregnancy diagnosis using ultrasonography does not significantly increase the risk of embryo and fetal loss in dairy cows. This work was supported by the research grant of the Chungbuk National University in 2007.

Proteomics and transcriptomics study reveals the utility of ISGs as novel molecules for early pregnancy diagnosis in dairy cows

2020 ◽  
Vol 140 ◽  
pp. 103148 ◽  
Author(s):  
Bibhudatta S.K. Panda ◽  
Sunil Kumar Mohapatra ◽  
Dheeraj Chaudhary ◽  
Mohanned Naif Alhussien ◽  
Rajeev Kapila ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Early Pregnancy ◽  
Pregnancy Diagnosis

Ultrasonography as Aid for Early Pregnancy Diagnosis in Zebu Cattle in a Natural Mating Programme

2006 ◽  
Vol 29 (1) ◽  
pp. 53-58 ◽  
Author(s):  
L. L. Galicia ◽  
K. S. Estrada ◽  
C. S. Galina ◽  
G. E. Pérez ◽  
J. J. Romero ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Zebu Cattle ◽  
Pregnancy Diagnosis ◽  
Natural Mating

Early pregnancy diagnosis in dairy cows using circulating nucleic acids

2013 ◽  
Vol 79 (1) ◽  
pp. 173-179 ◽  
Author(s):  
Jennifer Mayer ◽  
Jan T. Soller ◽  
Julia Beck ◽  
Vanessa Purwins ◽  
Wilhelm Wemheuer ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dairy Cows ◽  
Early Pregnancy ◽  
Pregnancy Diagnosis ◽  
Circulating Nucleic Acids

scholarly journals Early pregnancy diagnosis in ewes by means of transrectal realtime ultrasonography (short communication)

2001 ◽  
Vol 44 (1) ◽  
pp. 65-70
Author(s):  
A. D. Dinc ◽  
H. Erdem ◽  
I. Tasal ◽  
A. Semacan ◽  
A. Ergin
Keyword(s):  
Real Time ◽  
Early Pregnancy ◽  
Short Communication ◽  
Optimum Time ◽  
Predictive Values ◽  
Pregnancy Diagnosis ◽  
Linear Transducer ◽  
Group I ◽  
Natural Mating ◽  
Group Ii

Abstract. This study was performed to determine the optimum time for early pregnancy diagnosis with transrectal scanning in ewes. A total of 42 ewes was examined, using a real-time 5 MHz linear-transducer from day 19 to 29 (Group I, n = 22) and from day 30 to 44 (Group II, n = 20) after AI or natural mating. Ultrasonic results were compared with lambing records. Accuracy, sensitivity, specivity, positive and negative predictive values for these tests were 77.3 %, 84.2 %, 33.3 %, 88.9 %, 25.0 % in Group I, and 80.0 %, 74.3 %, 85.7 %, 90.9 % and 53.8 % in Group II, respectively. No significant differences were seen between the accuracy and positive predictive values of Group I and II. It was concluded, that the accuracy of pregnancy diagnosis by real-time transrectal ultrasonography can be high already 19 to 29 after AI or natural mating.

scholarly journals Duplex Quantitative Polymerase Chain Reaction of ISG15 and RSAD2 Increases Accuracy of Early Pregnancy Diagnosis in Dairy Cows

2019 ◽  
Vol 19 (2) ◽  
pp. 383-401
Author(s):  
Lei Cheng ◽  
Min Xiang ◽  
Xiuzhong Hu ◽  
Jie Yu ◽  
Yu Xia ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dairy Cows ◽  
Quantitative Pcr ◽  
Early Pregnancy ◽  
Quantitative Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Oligoadenylate Synthetase ◽  
Chain Reaction ◽  
Pregnancy Diagnosis ◽  
Polymerase Chain

AbstractEarly diagnosis of pregnancy is important in livestock production, but there is no reliable technology used for pregnancy diagnosis within the first three weeks after insemination. During early pregnancy, the expression of interferon-stimulating genes (ISGs) in peripheral blood leukocytes (PBL) is significantly increased. However, due to different strains, detection sample types, detection methods, threshold value, etc. the specific effectiveness of early pregnancy diagnosis using ISGs is worth further study. The purpose of this study was to test interferon-stimulated protein 15 (ISG15), 2'–5'-oligoadenylate synthetase 1 (OAS1) and radical S-adenosyl methionine domain containing 2 (RSAD2) for early pregnancy diagnosis in dairy cows. The expression of ISG15, OAS1, and RSAD2 in PBL of pregnant and non-pregnant heifers on days 0, 14, 18, 21 and 28 after artificial insemination (AI) was detected by fluorescence quantitative polymerase chain reaction (PCR). The sensitivity and specificity of the pregnancy diagnosis was analyzed using expression of these three genes separately or in combination by receiver operating characteristic curve. The combination with the highest accuracy used probe primers and duplex fluorescence quantitative PCR. The single quantitative PCR results showed that expression of ISG15, OAS1 and RSAD2 on day 18 after AI was significantly higher in pregnant than in non-pregnant cows. When these three genes were used separately, or in combination, for early pregnancy diagnosis, the sensitivity for the RSAD2 gene was 100%, and the combination of ISG15 with RSAD2 was 94.7%. The duplex quantitative PCR showed that, although the sensitivity of ISG15 alone was 100%, its specificity was only 88.2% (cut-off value 1.402). The sensitivity of RSAD2 alone was 89.5%, and the specificity was 88.2%; however, when the two genes were used in combination, the sensitivity, specificity and diagnostic cut-off value were consistent with the results of single quantitative PCR. These results indicated that a duplex quantitative PCR assay system for early pregnancy diagnosis in cows using ISG15 and RSAD2 was established. Simultaneous detection of expression of ISG15 and RSAD2 by duplex quantitative PCR can effectively improve the diagnostic accuracy for dairy cows.

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