scholarly journals Speciation Analysis of Ultratrace Chromium in Water by On-line Reaction/Concentration/Separation Method Using a Cation-Exchange Column

2013 ◽  
Vol 29 (8) ◽  
pp. 823-829 ◽  
Author(s):  
Sarenqiqige ◽  
Masaki ASHITOMI ◽  
Kazuhisa YOSHIMURA
Keyword(s):  
Cation Exchange ◽  
Speciation Analysis ◽  
Separation Method ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
On Line ◽  
Reaction Concentration

scholarly journals Structural similarity of chymopapain forms as indicated by circular dichroism

1989 ◽  
Vol 257 (1) ◽  
pp. 183-186 ◽  
Author(s):  
S Solis-Mendiola ◽  
R Zubillaga-Luna ◽  
A Rojo-Dominguez ◽  
A Hernandez-Arana
Keyword(s):  
Liquid Chromatography ◽  
High Resolution ◽  
Cation Exchange ◽  
Proteolytic Activity ◽  
Structural Similarity ◽  
Tertiary Structures ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Modified Proteins ◽  
Circular Dichroïsm

Four chymopapain forms were isolated by high-resolution liquid chromatography on a cation-exchange column. The three major forms possess nearly identical secondary and tertiary structures, as judged from their c.d. spectra; these components showed similar proteolytic activity and Mr values close to that of papain. The fourth isolated component seems to be a mixture of modified proteins.

Hemoglobin A1C by Hplc with the Pharmacia Mono S HR 5/N Cation-Exchange Column: Influence of Sample Protein Load on Optimal Chromatographic Conditions

1992 ◽  
Vol 38 (8) ◽  
pp. 1488-1490 ◽  
Author(s):  
J C Philcox ◽  
M R Haywood ◽  
A M Rofe
Keyword(s):  
Cation Exchange ◽  
Flow Rate ◽  
Chromatographic Separation ◽  
Hemoglobin A1c ◽  
Analysis Time ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Protein Load

Abstract The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.

Determination of haemoglobin A1c by liquid chromatography using a new cation-exchange column

2003 ◽  
Vol 791 (1-2) ◽  
pp. 73-83 ◽  
Author(s):  
Maria Beatriz de la Calle Guntiñas ◽  
René Wissiack ◽  
Guy Bordin ◽  
Adela Rosa Rodrı́guez
Keyword(s):  
Liquid Chromatography ◽  
Cation Exchange ◽  
Haemoglobin A1c ◽  
Exchange Column ◽  
Cation Exchange Column

ANALYSIS FOR SMALL AMOUNTS OF CALCIUM, MAGNESIUM, BARIUM, AND SULPHATE USING PHTHALEIN PURPLE

1956 ◽  
Vol 34 (7) ◽  
pp. 921-925 ◽  
Author(s):  
J. R. McCallum
Keyword(s):  
Cation Exchange ◽  
Distilled Water ◽  
Complexometric Method ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Calcium Magnesium

For the determination of sulphate ion in samples containing 3 to 20 mgm., it was found that the standard precipitation techniques did not give sufficiently accurate results, and were time consuming. Reference to the literature showed that with certain modifications a complexometric method proposed by Schwarzenbach etal. for samples containing 20 to 50 mgm. of sulphate was most likely to give accurate results rapidly. In this method the sample is passed through a cation exchange column and washed with distilled water into a flask. This solution is boiled and precipitated with excess standard disodium ethylenediaminetetracetate in the presence of alcohol and ammonia using phthalein purple indicator. Provided that the details of procedure described in this paper are closely adhered to, accuracy is usually within two per cent. Calcium, magnesium, and barium may also be titrated directly using the phthalein purple indicator.

Study of Extraction of Total Alkaloids from Caulophyllum Robustum

2013 ◽  
Vol 395-396 ◽  
pp. 601-604
Author(s):  
Jian Lan Suo
Keyword(s):  
Hydrochloric Acid ◽  
Cation Exchange ◽  
Acid Leaching ◽  
Optimal Conditions ◽  
Exchange Column ◽  
Total Alkaloids ◽  
Column Volume ◽  
Hydrochloric Acid Leaching ◽  
Cation Exchange Column ◽  
Caulophyllum Robustum

Objective: Optimizing the extraction of total alkaloids from Caulophyllum robustum. Methods: The taspine which is a type of alkaloids of Caulophyllum robustum is selected as the target index. First, developing the HPLC analysis protocol; second, using orthogonal design method to optimize the hydrochloric acid leaching protocol; third, using cation exchange column to isolate the alkaloids and examining the optimal conditions. Results: the optimal conditions of hydrochloric acid leaching protocol is using 7 column volume of 1% hydrochloric acid for 3 times. The materials are loaded onto the LSD001 cation exchange column with optimal pH 4 and flowrate 2ml/mL, the elution buffer is 10 column volume 5% hydrochloric acid. After desalting, the yield of alkaloids is 1.35% which includes 6.83% of taspine.

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