scholarly journals Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts

2016 ◽  
Vol 32 (2) ◽  
pp. 171-175 ◽  
Author(s):  
Jun WATANABE ◽  
Tomoyuki OKI ◽  
Jun TAKEBAYASHI ◽  
Hiroshi YADA ◽  
Manabu WAGAKI ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Antioxidant Capacities ◽  
Interlaboratory Validation ◽  
Lipophilic Antioxidant ◽  
Capacity Determination

scholarly journals Determination of the Antioxidative Activities of Herbs Harvested in Japan by Oxygen Radical Absorbance Capacity Methods

2016 ◽  
Vol 22 (2) ◽  
pp. 301-305
Author(s):  
Manabu Wakagi ◽  
Yuuki Taguchi ◽  
Jun Watanabe ◽  
Tasuku Ogita ◽  
Masao Goto ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity

scholarly journals Determination of antioxidant capacity in some foods by oxygen radical absorbance capacity (ORAC)

2021 ◽  
Vol 4 (3) ◽  
pp. 36-46
Author(s):  
Thuy Le Thi ◽  
Thuong Nguyen Nhu ◽  
Bang Nguyen Thi ◽  
Truc Quynh Do ◽  
Trang Vu Thi ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
High Selectivity ◽  
Peroxyl Radical ◽  
Fluorescence Decay ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Linear Coefficient ◽  
Vegetable Products ◽  
Trolox Equivalent

Oxygen radical absorbance capacity (ORAC) assay has been applied to determine the Hydrophilic – ORAC index in food. The method measures antioxidant scavenging activity against peroxyl radical induced by 2,2′-azobis(2-amidino-propane) dihydrochloride (AAPH) at 37ºC, used fluororescein as the fluorescent probe. The antioxidant capacity is measured by assemssing the fluorescence decay curve (AUC) of the sample as compared to that of the blank in which no antioxidant is present. Results expressed in ORAC units, equivalent to micromole Trolox per 100 grams sample (Trolox equivalent). The method was shown with high selectivity, a wide linear range, from 5 to 50µM Trolox with linear coefficient R2 = 0.9987. The recovery from 93.2% to 104.4% and repeatability (RSD) was less than 6.60%. The limits of detection and quantitation were 5 and 10µM Trolox, respectively. The method has been applied to determine of H-ORAC index in 65 samples including vegetables, fruits, vegetable products and health supplements with content ranging from 720 µM TE/100g to 310878 µM TE/100g.

Determination of Total Antioxidant Capacity by Oxygen Radical Absorbance Capacity (ORAC) Using Fluorescein as the Fluorescence Probe: First Action 2012.23

2013 ◽  
Vol 96 (6) ◽  
pp. 1372-1376 ◽  
Author(s):  
Boxin Ou ◽  
Tony Chang ◽  
Dejian Huang ◽  
Ronald L Prior
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Water Solubility ◽  
Fluorescence Probe ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Peroxyl Radicals ◽  
Phenolic Antioxidants ◽  
Total Antioxidant

Abstract An improved method for the measurement of oxygen radical absorbance capacity (ORAC) was developed and validated using fluorescein (3′,6′-dihydroxyspiro[isobenzofuran-1[3H], 9′[9H]-xanthen]-3-one) as a new fluorescence probe (ORACFL). Randomly methylated β-cyclodextrin (RMCD) was introduced as the water-solubility enhancer for lipophilic antioxidants. 7% RMCD (w/v) in 50% acetone–H2O mixture sufficiently solubilized vitamin E compounds and other lipophilic phenolic antioxidants in 75 mM phosphate buffer (pH 7.4). Results indicated that fluorescein shows excellent photostability under the plate reader conditions. This ORACFL was validated through linearity, precision, accuracy, and ruggedness. The validation results demonstrated that the ORACFL assay is reliable and robust. The mean of intraday and interday CVs were <15%; for hydrophilic ORAC, LOD and LOQ are 5 and 6.25 μM, respectively; for lipophilic ORAC, LOD and LOQ are 6.25 and 12.5 μM, respectively. It is concluded that unlike other popular methods, the ORACFL assay provides a direct measure of total antioxidant capacity against the peroxyl radicals.

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