Investigation of 5-(3-Trifluoromethylbenzylidene)thiazolidine-2,4-dione as a Matrix for Analyses of Biogenic Monoamine Transmitters Using MALDI-MS

Background: Tryptamine, a biogenic monoamine that is present in trace levels in the mammalian central nervous system, has probable roles as a neurotransmitter and/or a neuromodulator and may be associated with various neuropsychiatric disorders. One of the ways tryptamine may affect the body is by the competitive inhibition of the attachment of tryptophan to tryptophanyl tRNA synthetases. Methods: This study has explored the effects of tryptamine on growth of six yeast species (Saccharomyces cerevisiae, Candida glabrata, C. krusei, C. dubliniensis, C. tropicalis and C. lusitaniae) in media with glucose or ethanol as the carbon source, as well as recovery of growth inhibition by the addition of tryptophan. Results: Tryptamine was found to have an inhibitory effect on respiratory growth of all yeast species when grown with ethanol as the carbon source. Tryptamine also inhibited fermentative growth of Saccharomyces cerevisiae, C. krusei and C. tropicalis with glucose as the carbon source. In most cases the inhibitory effects were reduced by added tryptophan. Conclusion: The results obtained in this study are consistent with tryptamine competing with tryptophan to bind mitochondrial and cytoplasmic tryptophanyl tRNA synthetases in yeast: effects on mitochondrial and cytoplasmic protein synthesis can be studied as a function of growth with glucose or ethanol as a carbon source. Of the yeast species tested, there is variation in the sensitivity to tryptamine and the rescue by tryptophan. The current study suggests appropriate yeast strains and approaches for further studies.

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Characteristic MALDI-MS lipid profiles of Gir, Holstein and crossbred (Gir x Holstein) oocytes recovered by ovum pick-up

Livestock Science ◽

10.1016/j.livsci.2020.104380 ◽

2021 ◽

Vol 243 ◽

pp. 104380

Author(s):

Thamiris Vieira Marsico ◽

José Nélio de Sousa Sales ◽

Christina Ramires Ferreira ◽

Mateus José Sudano ◽

João Henrique Moreira Viana ◽

...

Keyword(s):

Lipid Profiles ◽

Maldi Ms

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Matrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS): basics and clinical applications

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2019-0868 ◽

2020 ◽

Vol 58 (6) ◽

pp. 883-896 ◽

Cited By ~ 3

Author(s):

Muhammad Zubair Israr ◽

Dennis Bernieh ◽

Andrea Salzano ◽

Shabana Cassambai ◽

Yoshiyuki Yazaki ◽

...

Keyword(s):

Mass Spectrometry ◽

Laser Desorption ◽

Maldi Mass Spectrometry ◽

Clinical Applications ◽

Clinical Diagnostics ◽

Maldi Ms ◽

Therapeutic Drug ◽

The Impact ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

AbstractBackgroundMatrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS) has been used for more than 30 years. Compared with other analytical techniques, it offers ease of use, high throughput, robustness, cost-effectiveness, rapid analysis and sensitivity. As advantages, current clinical techniques (e.g. immunoassays) are unable to directly measure the biomarker; rather, they measure secondary signals. MALDI-MS has been extensively researched for clinical applications, and it is set for a breakthrough as a routine tool for clinical diagnostics.ContentThis review reports on the principles of MALDI-MS and discusses current clinical applications and the future clinical prospects for MALDI-MS. Furthermore, the review assesses the limitations currently experienced in clinical assays, the advantages and the impact of MALDI-MS to transform clinical laboratories.SummaryMALDI-MS is widely used in clinical microbiology for the screening of microbial isolates; however, there is scope to apply MALDI-MS in the diagnosis, prognosis, therapeutic drug monitoring and biopsy imaging in many diseases.OutlookThere is considerable potential for MALDI-MS in clinic as a tool for screening, profiling and imaging because of its high sensitivity and specificity over alternative techniques.

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