scholarly journals Molecular detection and screening of Wolbachia infections in tropical bed bugs Cimex hemipterus (Hemiptera: Cimcidae) from Peninsular Malaysia Populations

Author(s):  
Mohd Hassan, N. H. ◽  
Ismail, D. E. ◽  
Seri Masran, S. N. A. ◽  
Ab Majid, A. H.
2020 ◽  
Author(s):  
Somayeh Bahrami ◽  
Ismaeil Alizadeh ◽  
Fatemeh Pazhoom ◽  
Susan Cork ◽  
Chukwunonso O.Nzelu ◽  
...  

Abstract Background: Bed bugs belong systematically to Order Hemiptera; Suborder Heteroptera; Family Cimicidae are of public health importance as ectoparasites of mammals and birds, however, only a few species are the putative ectoparasites of humans. Bed bugs are a wingless bloodsucking hemipterous bug (Cimex spp.) sometimes infesting houses and especially beds and feeding on human blood. Correct species identification is very important in order to design targeted strategies for surveillance and control of bed bugs in a given area. Methods: Adult bed bugs were collected from houses located in the southwest of Iran. The specimens were morphologically identified to the species level and then confirmed using molecular methods. Results: The mtDNA 16S rRNA sequences obtained from the specimens, and phylogenetic tree derived, showed that all the sequences belong to Cimex hemipterus. The Disparity Index among results showed that all the specimens were of a heterogeneous population. To the best of our knowledge, the leg structure of this species has not previously been documented and this is the first report of an open-closed rack system in the legs of C. hemipterus.Conclusions: Previous studies claimed that C. lectularius is the abundant species of Cimicids in Iran. But this investigation showed that C. hemipterus is the prevalent species in the southwest of Iran.


2020 ◽  
Vol 180 ◽  
pp. 105027 ◽  
Author(s):  
Shola David Ola-Fadunsin ◽  
Reuben Sunil Kumar Sharma ◽  
Donea Abdurazak Abdullah ◽  
Fufa Ido Gimba ◽  
Faez Firdaus Abdullah Jesse ◽  
...  

2020 ◽  
Vol 57 (3) ◽  
pp. 969-973 ◽  
Author(s):  
Juan Carlos Cambronero-Heinrichs ◽  
Laura Sofía Sánchez-Portilla ◽  
Ólger Calderón-Arguedas ◽  
Adriana Troyo

Abstract Cimex lectularius and Cimex hemipterus are the most common species of bedbugs that infest homes. Although case reports decreased substantially by the end of the 20th century, bed bugs, and especially C. lectularius, are currently suffering a resurgence mostly attributed to insecticide resistance, inadequate pest control, and increased travel. Here, we report, to the best of our knowledge, the first molecular confirmation of C. lectularius in Central America. Specimens were obtained from an apartment located in Heredia, Costa Rica. These specimens were identified morphologically as C. lectularius. The species identification was confirmed by amplifying and sequencing fragments of the cytochrome oxidase subunit I (COI) and the 16S rRNA (16S) genes. The phylogenetic analysis showed that the sequences obtained were more closely related to a C. lectularius mitochondrial complete genome sequence from China, with similarities of 98.84% (686/694) for COI and 98.97% (387/391) for 16S. The finding of C. lectularius in Costa Rica will require further investigation in order to determine the extent of current infestations and the susceptibility to insecticides, especially due to the impact that this species can have in human health, as well as the tourism industry in the region.


Author(s):  
Xin-Yeng Leong ◽  
Dae-Yun Kim ◽  
Kai Dang ◽  
G Veera Singham ◽  
Stephen L Doggett ◽  
...  

Abstract This study examined the presence of insecticide resistance in different developmental stages (adults, first instars, and eggs) of the tropical bed bug, Cimex hemipterus (F.) using several insecticide formulations. Adults and first instars of five strains (Queensland, Kuala Lumpur, Bukit Mertajam, Saujana, and Krystal Point) were evaluated using the surface contact method and compared with a susceptible strain (Monheim) of the common bed bug Cimex lectularius L. The insecticide formulations were used at their label rates in this study: Tandem (thiamethoxam [11.6%], lambda-cyhalothrin [3.5%]) at 183.96 mg/m2; Temprid SC (imidacloprid [21%], beta-cyfluthrin [10.5%]) at 106.13 mg/m2; Sumithion 20CS (fenitrothion [20%]) at 250 mg/m2; Pesguard FG161 (d-tetramethrin [4.4%], cyphenothrin [13.2%]) at 110 mg/m2; and Sumithrin 10SEC (d-phenothrin [10%]) at 100 mg/m2. Results showed a very high level of resistance to Pesguard FG161 (388.3 to >605.0 times) and Sumithrin (302.9 to >365.5 times) in all adults of the strains tested, whereas low to high levels of resistance were registered for Tandem (1.4–4.7 times), Temprid (7.3–16.7 times), and Sumithion (1.2–14.6 times) for adults of all bed bug strains. For first instars, resistance to the former two formulations were high to very high (31.4–118.1 times). In contrast, they showed lower resistance to Tandem, Temprid, and Sumithion (1.0–10.2 times). An immersion method used to test on bed bug eggs found high to very high resistance toward all tested formulations. Results demonstrate that the resistance level varies between bed bug developmental stages.


2020 ◽  
Vol 34 ◽  
pp. 100646 ◽  
Author(s):  
R. Potts ◽  
I. Molina ◽  
J.M. Sheele ◽  
J.E. Pietri
Keyword(s):  

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