scholarly journals Screening and Identification of LncRNAs Related to Villus Growth of Liaoning Cashmere Goats and Their Effects on Growth after FGF5 Treatment

2020 ◽  
Author(s):  
Mei Jin ◽  
Qin Feng Zhao ◽  
Ping Ni ◽  
Jun Piao ◽  
Ai Jing Piao
Keyword(s):  
Target Genes ◽  
Economic Value ◽  
Growth Cycle ◽  
Cashmere Goat ◽  
Lncrna Gene ◽  
Over Expression ◽  
Skin Cells ◽  
Non Coding Rna ◽  
Screening And Identification ◽  
Cashmere Goats

Abstract (Background)Liaoning cashmere goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results)This study investigated how LncRNA mediates the effects of FGF5 on the growth of Liaoning cashmere goats. By using RNA-seq sequencing technology, over-expression and interference lentiviral technology and qPCR, we demonstrated that treatment of skin cells with FGF5 inhibited the expression of LncRNA in cells, down-regulated the expression of the target genes CBS and CTH, and promoted the expression of related keratin genes k26, kap11.1. Then, overexpressing LncRNA in skin cells reversed the inhibiting effect of FGF5 on the target genes CBS and CTH which further inhibited the expression of k26, kap11.1. Finally, we demonstrated the inhibition of CBS and CTH and elevation k26 and kap11.1 genes when the expression level of LncRNA gene is downregulated using RNA interference. (Conclusions)Therefore, we believe that FGF5 can regulate the growth and development of cashmere goat hair by promoting the expression of related keratin and keratin-associated protein genes. This mechanism is achieved by inhibiting the expression of the LncRNA gene and then down-regulating the expression of the target genes CBS and CTH.

scholarly journals Screening and Identification of LncRNAs Related to Villus Growth of Liaoning Cashmere Goats and Their Effects on Growth after FGF5 Treatment

2020 ◽  
Author(s):  
Mei Jin ◽  
Qin Feng Zhao ◽  
Ping Ni ◽  
Jun Piao ◽  
Ai Jing Piao
Keyword(s):  
Target Genes ◽  
Economic Value ◽  
Growth Cycle ◽  
Cashmere Goat ◽  
Lncrna Gene ◽  
Skin Cells ◽  
Non Coding Rna ◽  
Screening And Identification ◽  
Cashmere Goats ◽  
Long Non Coding Rna

Abstract (Background)Liaoning Cashmere Goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results)We demonstrated that treatment of skin cells with FGF5 inhibited the expression of LncRNA in cells, down-regulated the expression of the target genes CBS and CTH, and promoted the expression of related keratin genes k26, kap11.1. Overexpressing LncRNA reversed the inhibiting effect of FGF5 on the target genes CBS and CTH. (Conclusions)we believe that FGF5 can regulate the growth and development of Cashmere Goat hair by promoting the expression of related keratin and keratin-associated protein genes. This mechanism is achieved by inhibiting the expression of the LncRNA gene and then down-regulating the expression of the target genes CBS and CTH.

scholarly journals Screening and Identification of LncRNAs Related to Villus Growth of Liaoning Cashmere Goats and Their Effects on Growth after FGF5 Treatment

2020 ◽  
Author(s):  
Mei Jin ◽  
Qin Feng Zhao ◽  
Ping Ni ◽  
Jun Piao ◽  
Ai Jing Piao
Keyword(s):  
Target Genes ◽  
Economic Value ◽  
Growth Cycle ◽  
Cashmere Goat ◽  
Lncrna Gene ◽  
Skin Cells ◽  
Non Coding Rna ◽  
Screening And Identification ◽  
Cashmere Goats ◽  
Long Non Coding Rna

Abstract (Background) Liaoning Cashmere Goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results) We demonstrated that treatment of skin cells with FGF5 inhibited the expression of LncRNA in cells, down-regulated the expression of the target genes CBS and CTH, and promoted the expression of related keratin genes k26, kap11.1. Overexpressing LncRNA reversed the inhibiting effect of FGF5 on the target genes CBS and CTH. (Conclusions) we believe that FGF5 can regulate the growth and development of Cashmere Goat hair by promoting the expression of related keratin and keratin-associated protein genes. This mechanism is achieved by inhibiting the expression of the LncRNA gene and then down-regulating the expression of the target genes CBS and CTH.

scholarly journals Screening and Identification of LncRNAs Related to Villus Growth of Liaoning Cashmere Goats and Their Effects on Growth after FGF5 Treatment

2019 ◽  
Author(s):  
Mei Jin ◽  
Qin Feng Zhao ◽  
Ping Ni ◽  
Jun Piao ◽  
Ai Jing Piao
Keyword(s):  
Hair Follicle ◽  
Target Genes ◽  
Economic Value ◽  
Research Direction ◽  
Growth Cycle ◽  
Follicle Development ◽  
Keratin Gene ◽  
Cashmere Goats ◽  
Hair Follicle Development ◽  
New Research

Abstract Abstract : (Background)Liaoning cashmere goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results)This study investigated how LncRNA mediates the effects of FGF5 on the growth of Liaoning cashmere goats. We screened for LncRNA related to hair follicle development and villus growth by RNA-seq sequencing. GO and pathway analysis determined that the optimal treatment conditions for FGF5 drugs are 10 -4 g/L for 72h (F4_72h). The expression levels of CBS, CTH, keratin gene K26, KAP11.1 were studied when overexpressing and interfering with LncRNA. (Conclusions)To our knowledge, this is the first study on how LncRNA regulates villi growth by regulating target genes and keratin genes in the amino acid metabolic pathway; it is also the first to open a new research direction for studying the mechanism of FGF5 in regulating hair follicle development and villus growth.

scholarly journals Screening of cashmere fineness-related genes and their ceRNA network construction in cashmere goats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Taiyu Hui ◽  
Yuanyuan Zheng ◽  
Chang Yue ◽  
Yanru Wang ◽  
Zhixian Bai ◽  
...  
Keyword(s):  
Circular Rna ◽  
Differentially Expressed ◽  
Transcriptional Level ◽  
Cashmere Goat ◽  
Cerna Network ◽  
Non Coding Rna ◽  
Potential Basis ◽  
Cashmere Goats ◽  
Regulatory Effects ◽  
Long Non Coding Rna

AbstractCompetitive endogenous RNA (ceRNA) is a transcript that can be mutually regulated at the post-transcriptional level by competing shared miRNAs. The ceRNA network connects the function of protein-encoded mRNA with the function of non-coding RNA, such as microRNA (miRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA). However, compared with the ceRNA, the identification and combined analysis of lncRNAs, mRNAs, miRNAs, and circRNAs in the cashmere fineness have not been completed. Using RNA-seq technology, we first identified the miRNAs presented in Liaoning Cashmere Goat (LCG) skin, and then analyzed the mRNAs, lncRNAs, circRNAs expressed in LCG and Inner Mongolia cashmere goat (MCG) skin. As a result, 464 known and 45 new miRNAs were identified in LCG skin. In LCG and MCG skin, 1222 differentially expressed mRNAs were identified, 170 differentially expressed lncRNAs and 32 differentially expressed circRNAs were obtained. Then, qRT-PCR was used to confirm further the representative lncRNAs, mRNAs, circRNAs and miRNAs. In addition, miRanda predicted the relationships of ceRNA regulatory network among lncRNAs, circRNAs, miRNAs and mRNAs, the potential regulatory effects were investigated by Go and KEGG analysis. Through the screening and analysis of the results, the ceRNA network regulating cashmere fineness was constructed. LncRNA MSTRG14109.1 and circRNA452 were competed with miRNA-2330 to regulated the expression of TCHH, KRT35 and JUNB, which may provide a potential basis for further research on the process of regulating the cashmere fineness.

scholarly journals Genome-wide detection and sequence conservation analysis of long non-coding RNA during hair follicle cycle of yak

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiaolan Zhang ◽  
Qi Bao ◽  
Congjun Jia ◽  
Chen Li ◽  
Yongfang Chang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Expression Profile ◽  
Target Genes ◽  
Differential Expression Analysis ◽  
Wnt Signaling Pathway ◽  
Hair Follicles ◽  
Sequence Conservation ◽  
Cashmere Goat ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Background Long non-coding RNA (lncRNA) as an important regulator has been demonstrated playing an indispensable role in the biological process of hair follicles (HFs) growth. However, their function and expression profile in the HFs cycle of yak are yet unknown. Only a few functional lncRNAs have been identified, partly due to the low sequence conservation and lack of identified conserved properties in lncRNAs. Here, lncRNA-seq was employed to detect the expression profile of lncRNAs during the HFs cycle of yak, and the sequence conservation of two datasets between yak and cashmere goat during the HFs cycle was analyzed. Results A total of 2884 lncRNAs were identified in 5 phases (Jan., Mar., Jun., Aug., and Oct.) during the HFs cycle of yak. Then, differential expression analysis between 3 phases (Jan., Mar., and Oct.) was performed, revealing that 198 differentially expressed lncRNAs (DELs) were obtained in the Oct.-vs-Jan. group, 280 DELs were obtained in the Jan.-vs-Mar. group, and 340 DELs were obtained in the Mar.-vs-Oct. group. Subsequently, the nearest genes of lncRNAs were searched as the potential target genes and used to explore the function of DELs by GO and KEGG enrichment analysis. Several critical pathways involved in HFs development such as Wnt signaling pathway, VEGF signaling pathway, and signaling pathways regulating pluripotency of stem cells, were enriched. To further screen key lncRNAs influencing the HFs cycle, 24 DELs with differ degree of sequence conservation were obtained via a comparative analysis of partial DELs with previously published lncRNA-seq data of cashmere goat in the HFs cycle using NCBI BLAST-2.9.0+, and 3 DELs of them were randomly selected for further detailed analysis of the sequence conservation properties. Conclusions This study revealed the expression pattern and potential function of lncRNAs during HFs cycle of yak, which would expand the knowledge about the role of lncRNAs in the HFs cycle. The findings related to sequence conservation properties of lncRNAs in the HFs cycle between the two species may provide valuable insights into the study of lncRNA functionality and mechanism.

scholarly journals Skin transcriptome reveals the periodic changes in genes underlying cashmere (ground hair) follicle transition in Cashmere goats

2020 ◽  
Author(s):  
Feng Yang ◽  
Zhihong Liu ◽  
Meng Zhao ◽  
Qing Mu ◽  
Tianyu Che ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Growth Period ◽  
Growth Cycle ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Differential Gene ◽  
Cashmere Goats ◽  
Key Genes ◽  
Hair Follicle Growth ◽  
Resting Period

Abstract Background: Cashmere goats make an outstanding contribution to the livestock textile industry and their cashmere is famous for its slenderness and softness and has been extensively studied. However, there are few reports on the molecular regulatory mechanisms of the secondary hair follicle growth cycle in cashmere goats. In order to explore the regular transition through the follicle cycle and the role of key genes in this cycle, we used a transcriptome sequencing technique to sequence the skin of Inner Mongolian cashmere goats during different months. We analyzed the variation and difference in genes throughout the whole hair follicle cycle. We then verified the regulatory mechanism of the cashmere goat secondary hair follicle growth cycle using fluorescence quantitative PCR. Results: The growth cycle of cashmere hair could be divided into three distinct periods: a growth period (March–September), a regression period (September–December), and a resting period (December–March). The results of differential gene analyses showed that March was the most significant month. Cluster analysis of gene expression throughout the whole growth cycle further supported the key nodes of the three periods of cashmere growth, and the differential gene expression of keratin corresponding to the ground haircashmere growth cycle further supported the results from tissue slices. Quantitative fluorescence analysis showed that KAP3-1, KRTAP 8-1, and KRTAP 24-1 genes had close positive correlation with the cashmere growth cycle, and their regulation was consistent with the growth cycle of cashmere. Conclusion: The growth cycle of cashmere cashmere could be divided into three distinct periods: a growth period (March–September), a regression period (September–December) and a resting period (December–March). March was considered to be the beginning of the cycle. KAP and KRTAP showed close positive correlation with the growth cycle of secondary hair follicle cashmere growth, and their regulation was consistent with the cashmere growth cycle. But hair follicle development-related genes are expressed earlier than cashmere growth, indicating that cycle regulation could alter the temporal growth of cashmere. This study laid a theoretical foundation for the study of the cashmere development cycle and provided evidence for key genes during transition through the cashmere cycle. Our study provides a theoretical basis for cashmere goat breeding.

scholarly journals Genome-wide detection and sequence conservation analysis of long non-coding RNA during hair follicle cycle of yak

2020 ◽  
Author(s):  
Xiaolan Zhang ◽  
Qi Bao ◽  
Congjun Jia ◽  
Chen Li ◽  
Yongfang Chang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Expression Profile ◽  
Target Genes ◽  
Differential Expression Analysis ◽  
Wnt Signaling Pathway ◽  
Hair Follicles ◽  
Sequence Conservation ◽  
Cashmere Goat ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Background: Long non-coding RNA (lncRNA) as an important regulator has been demonstrated playing an indispensable role in the biological process of hair follicles (HFs) growth. However, their function and expression profile in the HFs cycle of yak are yet unknown. Only a few functional lncRNAs have been identified, partly due to the low sequence conservation and lack of identified conserved properties in lncRNAs. Here, lncRNA-seq was employed to detect the expression profile of lncRNAs during the HFs cycle of yak, and the sequence conservation of two datasets between yak and cashmere goat during the HFs cycle was analyzed. Results: A total of 2884 lncRNAs were identified in 5 phases (Jan., Mar., Jun., Aug., and Oct.) during the HFs cycle of yak. Then, differential expression analysis between 3 phases (Jan., Mar., and Oct.) was performed, revealing that 198 differentially expressed lncRNAs (DELs) were obtained in the Oct.-vs-Jan. group, 280 DELs were obtained in the Jan.-vs-Mar. group, and 340 DELs were obtained in the Mar.-vs-Oct. group. Subsequently, the nearest genes of lncRNAs were searched as the potential target genes and used to explore the function of DELs by GO and KEGG enrichment analysis. Several critical pathways involved in HFs development such as Wnt signaling pathway, VEGF signaling pathway, and signaling pathways regulating pluripotency of stem cells, were enriched. To further screen key lncRNAs influencing the HFs cycle, 24 DELs with differ degree of sequence conservation were obtained via a comparative analysis of partial DELs with previously published lncRNA-seq data of cashmere goat in the HFs cycle using NCBI BLAST-2.9.0+, and 3 DELs of them were randomly selected for further detailed analysis of the sequence conservation properties.Conclusions: This study revealed the expression pattern and potential function of lncRNAs during HFs cycle of yak, which would expand the knowledge about the role of lncRNAs in the HFs cycle. The findings related to sequence conservation properties of lncRNAs in the HFs cycle between the two species may provide valuable insights into the study of lncRNA functionality and mechanism.

scholarly journals Screening the key genes of hair follicle growth cycle in Inner Mongolian Cashmere goat based on RNA sequencing

2020 ◽  
Vol 63 (1) ◽  
pp. 155-164
Author(s):  
Rui Su ◽  
Gao Gong ◽  
Lingtian Zhang ◽  
Xiaochun Yan ◽  
Fenghong Wang ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Rna Sequencing ◽  
Growth Cycle ◽  
Raw Material ◽  
High Price ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Cashmere Goats ◽  
Hair Follicle Growth ◽  
Upregulated Genes

Abstract. Inner Mongolian Cashmere goat is an excellent local breed selected for the dual-purpose of cashmere and meat. There are three lines of Inner Mongolian Cashmere goat: Erlangshan, Alashan and Aerbasi. Cashmere is a kind of precious textile raw material with a high price. Cashmere is derived from secondary hair follicle (SHF), while hair is derived from primary hair follicle (PHF). The growth cycle of SHF of cashmere goat is 1 year, and it can be divided into three different stages: anagen, catagen and telogen. In this study, we tried to find some important influence factors of SHF growth cycle in skin tissue from Inner Mongolian Cashmere goats by RNA sequencing (RNA-Seq). Three female Aerbasi Inner Mongolian Cashmere goats (2 years old) were used as experimental samples in this study. Skin samples were collected in September (anagen), December (catagen) and March (telogen) at dorsal side from cashmere goats. Results showed that over 511 396 044 raw reads and 487 729 890 clean reads were obtained from sequence data. In total, 51 different expression genes (DEGs) including 29 downregulated genes and 22 upregulated genes were enriched in anagen–catagen comparing group. The 443 DEGs contained 117 downregulated genes and 326 upregulated genes that were enriched in catagen–telogen comparing group. In telogen–anagen comparing group, 779 DEGs were enriched including 582 downregulated genes and 197 upregulated genes. The result of gene ontology (GO) annotation showed that DEGs are in different growth cycle periods, and enriched GO items are mostly related to the transformation of cell and protein. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment result indicated that metabolic process has a great impact on SHF growth cycle. Based on the results of a comprehensive analysis of differentially expressed genes, GO enrichment and KEGG enrichment, we found that FGF5, FGFR1 and RRAS had an effect on the hair follicle growth cycle. The results of this study may provide a theoretical basis for further research on the growth and development of SHF in Inner Mongolian Cashmere goats.

scholarly journals Genome-wide detection and sequence conservation analysis of long non-coding RNA during hair follicle cycle of yak

2020 ◽  
Author(s):  
Xiaolan Zhang ◽  
Qi Bao ◽  
Congjun Jia ◽  
Chen Li ◽  
Yongfang Chang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Expression Profile ◽  
Target Genes ◽  
Differential Expression Analysis ◽  
Wnt Signaling Pathway ◽  
Hair Follicles ◽  
Sequence Conservation ◽  
Cashmere Goat ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Background: Long non-coding RNA (lncRNA) as an important regulator has been demonstrated playing an indispensable role in the biological process of hair follicles (HFs) growth. However, their function and expression profile in the HFs cycle of yak are yet unknown. Only a few functional lncRNAs have been identified, partly due to the low sequence conservation and lack of identified conserved properties in lncRNAs. Here, lncRNA-seq was employed to detect the expression profile of lncRNAs during the HFs cycle of yak, and the sequence conservation of two datasets between yak and cashmere goat during the HFs cycle was analyzed. Results: A total of 2884 lncRNAs were identified in 5 phases (Jan., Mar., Jun., Aug., and Oct.) during the HFs cycle of yak. Then, differential expression analysis between 3 phases (Jan., Mar., and Oct.) was performed, revealing that 198 differentially expressed lncRNAs (DELs) were obtained in the Oct.-vs-Jan. group, 280 DELs were obtained in the Jan.-vs-Mar. group, and 340 DELs were obtained in the Mar.-vs-Oct. group. Subsequently, the nearest genes of lncRNAs were searched as the potential target genes and used to explore the function of DELs by GO and KEGG enrichment analysis. Several critical pathways involved in HFs development such as Wnt signaling pathway, VEGF signaling pathway, and signaling pathways regulating pluripotency of stem cells, were enriched. To further screen key lncRNAs influencing the HFs cycle, 24 DELs with differ degree of sequence conservation were obtained via a comparative analysis of partial DELs with previously published lncRNA-seq data of cashmere goat in the HFs cycle using NCBI BLAST-2.9.0+, and 3 DELs of them were randomly selected for further detailed analysis of the sequence conservation properties.Conclusions: This study revealed the expression pattern and potential function of lncRNAs during HFs cycle of yak, which would expand the knowledge about the role of lncRNAs in the HFs cycle. The findings related to sequence conservation properties of lncRNAs in the HFs cycle between the two species may provide valuable insights into the study of lncRNA functionality and mechanism.

scholarly journals Skin transcriptome reveals the periodic changes in genes underlying cashmere (ground hair) follicle transition in Cashmere goats

2020 ◽  
Author(s):  
Feng Yang ◽  
Zhihong Liu ◽  
Meng Zhao ◽  
Qing Mu ◽  
Tianyu Che ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Growth Period ◽  
Growth Cycle ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Differential Gene ◽  
Cashmere Goats ◽  
Key Genes ◽  
Hair Follicle Growth ◽  
Resting Period

Abstract Background: Cashmere goats make an outstanding contribution to the livestock textile industry and their cashmere is famous for its slenderness and softness and has been extensively studied. However, there are few reports on the molecular regulatory mechanisms of the secondary hair follicle growth cycle in cashmere goats. In order to explore the regular transition through the follicle cycle and the role of key genes in this cycle, we used a transcriptome sequencing technique to sequence the skin of Inner Mongolian cashmere goats during different months. We analyzed the variation and difference in genes throughout the whole hair follicle cycle. We then verified the regulatory mechanism of the cashmere goat secondary hair follicle growth cycle using fluorescence quantitative PCR. Results: The growth cycle of cashmere hair could be divided into three distinct periods: a growth period (March–September), a regression period (September–December), and a resting period (December–March). The results of differential gene analyses showed that March was the most significant month. Cluster analysis of gene expression throughout the whole growth cycle further supported the key nodes of the three periods of cashmere growth, and the differential gene expression of keratin corresponding to the ground haircashmere growth cycle further supported the results from tissue slices. Quantitative fluorescence analysis showed that KAP3-1, KRTAP 8-1, and KRTAP 24-1 genes had close positive correlation with the cashmere growth cycle, and their regulation was consistent with the growth cycle of cashmere. Conclusion: The growth cycle of cashmere cashmere could be divided into three distinct periods: a growth period (March–September), a regression period (September–December) and a resting period (December–March). March was considered to be the beginning of the cycle. KAP and KRTAP showed close positive correlation with the growth cycle of secondary hair follicle cashmere growth, and their regulation was consistent with the cashmere growth cycle. But hair follicle development-related genes are expressed earlier than cashmere growth, indicating that cycle regulation could alter the temporal growth of cashmere. This study laid a theoretical foundation for the study of the cashmere development cycle and provided evidence for key genes during transition through the cashmere cycle. Our study provides a theoretical basis for cashmere goat breeding.

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