scholarly journals Evaluation of Mutagenesis, Necrosis and Apoptosis induced by Omeprazole in Stomach Cells of Patients with Gastritis

2021 ◽  
Author(s):  
Ana Maria Oliveira Ferreira da Mata ◽  
Marcia Fernanda Correia Jardim Paz ◽  
Ag-Anne Pereira Melo de Menezes ◽  
Antonielly Campinho dos Reis ◽  
Bruna da Silva Souza ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Inflammatory Lesion ◽  
Gastric Ulcers ◽  
Group 4 ◽  
Group 6 ◽  
Group 5 ◽  
Group 2 ◽  
Positive Correlations ◽  
Getulio Vargas ◽  
H Pylori

Abstract Background: Gastritis is a superficial and prevalent inflammatory lesion that is considered a public health concernoncecause gastric ulcers and gastric cancer, especially due to Helicobacter pylori infection. Proton pump inhibitors, such as omeprazole, are the most widely used drugs in order to treat this illness. The aim of the study was evaluating the cytogenetic effects of omeprazole in stomach epithelial cells of patients with gastritis in presence and absence of H. pylori, using to this the application of cytogenetic biomarkers and measurements of catalse and superoxide dismutase. Methods: The study included 152 patients from the Gastroenterology Outpatient Clinic of Hospital Getúlio Vargas, Teresina - PI, that reported the continuous and prolonged use of omeprazole in doses of 20, 30 and 40 mg/kg. The participants were divided into groups: (1) patients without gastritis (n = 32); (2) patients without gastritis but with use of OME (n = 24); (3) patients with gastritis (n = 26); (4) patients with gastritis undergoing OME therapy (n = 26); (5) patients with gastritis and H. pylori (n = 22) and (6) patients with gastritis and H. pylori on OME therapy (n = 22). Results: OME induces cytogenetic risks in the epithelium of the stomach due to the formation of micronuclei (group 6> 1,2,3,4,5; group 5> 1,2,3; group 4> 1,2,3); bridges (groups 4 and 6> 1,2,3,5 and group 2> 3,5); buds (groups 2,4,6>, 1,3,5); binucleated cells (group 6> 1,2,3,4,5; group 4> 1,2,3); groups 2 and 3> 1); picnoses (group 6> 1,2,3,4,5), groups 2 and 5> 1,3; group 4> 1,2,3,5); cariorrexis (groups 6 and 4> 1,2,3,5; groups 2,3,5> 1) and karyolysis (groups 2,4, and 6> 1,3,5; groups 3 and 5> 1). These data show that omeprazole induces cytogenetic risks, especially due to infection with H. pylori, thus indicating the clastogenic and/or aneugenic effects, chromosomes changing, gene expression, cytotoxicity and apoptosis. Conclusions: These risks can be attributed to several mechanisms that are still unclear, including oxidative damage, as observed by increases in catalase and superoxide dismutase. Positive correlations between these antioxidant enzymes were obtained with the formation of micronuclei, and negative for picnoses. Thus, the continuous and prolonged use of omeprazole induces genetic instability, which can be monitored, in cytogenetic analyzes, as anticipation for cancer, especially gastric.

scholarly journals Association between serum oestradiol level on the hCG administration day and neonatal birthweight after IVF-ET among 3659 singleton live births

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yu Liu ◽  
Jing Li ◽  
Wanyu Zhang ◽  
Yihong Guo
Keyword(s):  
Birth Weight ◽  
Ovarian Stimulation ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
The Impact ◽  
Ivf Et ◽  
Group 1

AbstractOestradiol, an important hormone in follicular development and endometrial receptivity, is closely related to clinical outcomes of fresh in vitro fertilization-embryo transfer (IVF-ET) cycles. A supraphysiologic E2 level is inevitable during controlled ovarian hyper-stimulation (COH), and its effect on the outcome of IVF-ET is controversial. The aim of this retrospective study is to evaluate the association between elevated serum oestradiol (E2) levels on the day of human chorionic gonadotrophin (hCG) administration and neonatal birthweight after IVF-ET cycles. The data of 3659 infertile patients with fresh IVF-ET cycles were analysed retrospectively between August 2009 and February 2017 in First Hospital of Zhengzhou University. Patients were categorized by serum E2 levels on the day of hCG administration into six groups: group 1 (serum E2 levels ≤ 1000 pg/mL, n = 230), group 2 (serum E2 levels between 1001 and 2000 pg/mL, n = 524), group 3 (serum E2 levels between 2001 and 3000 pg/mL, n = 783), group 4 (serum E2 levels between 3001 and 4000 pg/mL, n = 721), group 5 (serum E2 levels between 4001 and 5000 pg/mL, n = 548 ), and group 6 (serum E2 levels > 5000 pg/mL, n = 852). Univariate linear regression was used to evaluate the independent correlation between each factor and outcome index. Multiple logistic regression was used to adjust for confounding factors. The LBW rates were as follows: 3.0% (group 1), 2.9% (group 2), 1.9% (group 3), 2.9% (group 4), 2.9% (group 5), and 2.0% (group 6) (P = 0.629), respectively. There were no statistically significant differences in the incidences of neonatal LBW among the six groups. We did not detect an association between peak serum E2 level during ovarian stimulation and neonatal birthweight after IVF-ET. The results of this retrospective cohort study showed that serum E2 peak levels during ovarian stimulation were not associated with birth weight during IVF cycles. In addition, no association was found between higher E2 levels and increased LBW risk. Our observations suggest that the hyper-oestrogenic milieu during COS does not seem to have adverse effects on the birthweight of offspring after IVF. Although this study provides some reference, the obstetric-related factors were not included due to historical reasons. The impact of the high estrogen environment during COS on the birth weight of IVF offspring still needs future research.

EFFECT OF TREATMENT WITH STREPTOKINASE AND HEPARIN ON FIBRINOGEN, FIBRIN AND RELATED PROTEINS IN ACUIE MYOCARDIAL INFARCTION (/ME) PATIENTS

1987 ◽  
Author(s):  
V Vila ◽  
E Reganon ◽  
J Aznar ◽  
V Lacueva ◽  
M Ruano
Keyword(s):  
Degradation Products ◽  
Heparin Therapy ◽  
Heparin Group ◽  
Normal Value ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

The properties of fibrinogen and fibrin, the levels of fibrincpeptide A (FPA) and fibrin(ogen) degradation products (FDP) were studied in 34 patients with AMI who were undergoing thrombolytic and heparin therapy. They were classified into 6 groups accordingto their stage of treatment: group 1, before intravenous administration of 800.000 U streptokinase over 30 min; group 2, after a<Mnistraticn of SK but before adninistraticn of heparin; group 3, during 24 h ofthe 5 ng/h heparin continuous infusion; group 4, during 48-72 h of the 16.6 ng/h heparin continuous infhsion; group 5, after 1 week of administration of SK and with a bolus inyection of 50 rg heparin every 4 h; group 6, patients who were undergoing only heparin treatment. The Fg 1/ Fg II ratio varies during treatment with SK and heparin. In group 1 a sligjnt increase (2.5) is observed. Group 2 shows a significantdecrease (0.6) as a result of fibrinolysis. In group3 the ratio reaches normal value (1.8) while in the fourth group it is twice the normal value (4). The value for group 5 is nearly normal (2.1), and in group 6 it reaches values similar to those obtained in group 4, which implies that the rise in the Fgl/Fgll ratio is not a result of fibrinolytic treatment. TheFPA level shows and increase in patients with AMI (group 1,126 ng/ml). When SK treatment is applied (group 2), FPA decreases to 52 ng/ml. Later treatment with heparin (group-3, 82; group-4, 44 and group-5, 81ng/ml) does not neutralize thrcmbinic activity. Patients treated only with heparin (group 6) show an FPAvalue of 19 ng/ml, which is lower than in the other groups. All of this indicates that thrombin is activated after fibrinolytic treatment. FDP values show asignificant increase in the six groups (1, 53; 2, 430; 3, 128; 4, 270; 5, 139 and 6, 141 ug/ml), which indicates that during treatment with heparin the fibrinolytic activity persists. he formation of highly cross-linked fibrin is altered in groups 1,2,3 and 4,as a consequence of circulating FDP effect and fibrincgeno- lysis.The permeability of the fibrin clotdecreases in groups 1 (0.42), 2 (1.3), 4 (1.1) and 5(0.5 ml/s/ng) and increases in group 2 (23.2 ml/s/nig) with respect to the normal plasma value (3.2 ml/s/nrg). The decrease in permeability must be related to the existence of hypercoagulability resistant to heparinization. FPA values, tine Fgl/Fgll ratio, andfibrin permeability can be used to evaluate the degree of thrcmbin activity during thrombolytic treatmentinAMI.

Estudo de fraturas nos taludes de corte, em rocha vulcânica, ao longo da BR 158, Santa Maria, RS

1999 ◽  
Vol 21 (21) ◽  
pp. 97
Author(s):  
Andrea V. Nummer ◽  
Luis Eduardo S. Robaina ◽  
Marcos Geovane Berger
Keyword(s):  
Conditioning Factors ◽  
Weathering Processes ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Road Data ◽  
Santa Maria ◽  
Group 1

Weathering processes and jointing degree are the conditioning factors for rock falI.The fractured volcanic lithologies from Santa Maria, RS, Brazil, were studied along the BR 158 road. Data was gathered through the Scanline method from BRADY & BROWN (1985) and analyzed by vector statistics.Six main jointing groups were defined, associated to rock cooling and shrinking. Main attitudes are: Group 1: 348;83; Group 2: 174;82; Group 3: 85;76; Group 4: 265;75; Group 5: 300;42 and Group 6: 121 ;53.

Dentin Bond Strength of Composites with Self-etching Adhesives Using LED Curing Lights

2007 ◽  
Vol 8 (5) ◽  
pp. 34-42 ◽  
Author(s):  
Yonca Korkmaz ◽  
Nuray Attar
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Significance Level ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Curing Light ◽  
Group 5 ◽  
Group 2 ◽  
Self Etch

Abstract Aim The purpose of this study was to investigate the effect of light-emitting diode (LED) light curing units (LCUs) compared with halogen LCUs on the shear bond strength (SBS) of one nanofill composite (Filtek Supreme) and one microhibrid composite (Artemis) with self-etch adhesives. Methods and Materials The buccal surfaces of 60 non-carious extracted human molars were flattened to expose dentin and, subsequently, polished for 60 seconds with 600-grit wet silicon carbide abrasive paper. Specimens were assigned into six groups (n=10) according to composite material, self-etch adhesive, and curing light used as follows: Group 1: Adper Prompt L-Pop (AP) and Filtek Supreme (FS) using an Elipar Free Light (EFL); Group 2: AP and FS using an Elipar Free Light 2 (EFL2); Group 3: AP and FS using a Hilux Expert (HE) light, Group 4: AdheSE (AS)+Artemis (AR) using an EFL; Group 5: AS+AR using an EFL2; and Group 6: AS+AR using a HE light. The specimens were thermocycled for 500 cycles (5°C–55°C) and then loaded to failure in a Zwick universal testing machine at a crosshead speed of 5 mm/minute. SBS values were calculated as megapascals (MPa) and statistically analyzed using the one-way analysis of variance (ANOVA) test at a significance level of 0.05. Results Mean SBS (± standard deviations) values were as follows: Group1: 15.99±5.18; Group 2: 18.76±6.71; Group 3: 17.70±5.04; Group 4: 16.93±3.99; Group 5: 18.01±5.19, and Group 6: 17.46±5.40. There were no statistically significant differences for SBS to dentin among the groups tested. Conclusion The LED curing lights used in the study seem to be comparable with the halogen curing light for nanofill and microhybrid composites used in conjunction with self-etching systems in dentin. The EFL2 reduces curing time, which can be considered as an advantage. Citation Korkmaz Y, Attar N. Dentin Bond Strength of Composites with Self-etching Adhesives Using LED Curing Lights. J Contemp Dent Pract 2007 July;(8)5:034-042.

Effect of Qmix and Other Irrigants on Dentin Adhesives in Pulp Chambers of Primary Teeth: SEM Study

2017 ◽  
Vol 41 (5) ◽  
pp. 363-367
Author(s):  
Sidharth Kapoor ◽  
Naveen Manuja ◽  
Seema Chaudhary ◽  
Harsimran Kaur ◽  
Chaitra TR ◽  
...  
Keyword(s):  
Normal Saline ◽  
Saline Group ◽  
Primary Molars ◽  
Pulp Chamber ◽  
Significance Level ◽  
Group 4 ◽  
Two Samples ◽  
Group 6 ◽  
Group 5 ◽  
Group 2

Objective: To compare the effect of different endodontic irrigants on microleakage of adhesives used within pulp chamber of primary molars. Study Design: 72 Primary molars were divided into 6 groups, according to 3 irrigants and 2 adhesives used. After de-roofing the pulp chamber, pulp was extirpated. In 36 samples, pulp chambers were bonded with Xeno V+ after irrigation with either QMix (Group1); 17%EDTA+5%NaOCl (Group2) or normal saline (Group3) and in other half samples, pulp chambers were bonded with SinglebondUniversal after irrigation with either QMix (Group 4); 17%EDTA+5% NaOCl (Group5) or normal saline (Group 6). All the samples were restored with Filtek Z350. Ten teeth from each group were assessed for dye penetration. Two samples in each group were viewed under scanning electron microscope. Data was statistically analyzed using Mann–Whitney and Kruskal Wallis tests at a significance level of P &lt; 0.05. Results: Mean microleakage scores were: Group 1-1.5±0.70, Group 2-1.6±.51, Group 3-2.4±0.96, Group 4-1.2±0.42, Group 5-1.2±0.42, Group 6-1.1±0.32. Conclusions: Irrigation with QMix significantly reduced the microleakage of XenoV+ but had no significant effect on microleakage of SinglebondUniversal. Irrigation with EDTA/NaOCl or QMix had no detrimental effect on the sealing ability of either of the adhesive tested.

Evaluation of the Effect of Different Ferrule Designs on the Fracture Resistance of Endodontically Treated Maxillary Central Incisors Incorporating Fiber Posts, Composite Cores and Crown Restorations

2007 ◽  
Vol 8 (7) ◽  
pp. 62-69 ◽  
Author(s):  
Emre Ozel ◽  
Idil Dikbas ◽  
Jale Tanalp ◽  
Temel Koksal ◽  
Mustafa Ersoy
Keyword(s):  
Fracture Resistance ◽  
Quartz Fiber ◽  
Group 4 ◽  
Fiber Posts ◽  
Significant Difference ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Abstract Aim The purpose of this study was to evaluate the fracture resistance of endodontically treated maxillary central incisors restored with quartz fiber posts, composite cores, and crowns when different types of ferrule designs were incorporated. Methods and Materials Sixty maxillary incisors were divided into six groups: Group 1 (control): teeth with root canal treatments having a full crown prosthesis; Group 2: teeth with a 2 mm circumferential ferrule; Group 3: teeth with a 2 mm ferrule only in the vestibular region; Group 4: teeth with a 2 mm ferrule only in the palatal region; Group 5: teeth with a 2 mm ferrule in the vestibular and palatal region, having cavities in both proximal areas; and Group 6: teeth with no ferrule. The teeth in the experimental groups were restored with quartz fiber posts-composite cores and full metallic crowns. All experimental teeth were subjected to an increasing compressive force with a crosshead speed of 1 mm/min, until fracture occurred. Results The median fracture values of groups were as follows: Group 1: 574.4 N, Group 2: 472.4 N, Group 3: 474.3 N, Group 4: 480.7 N, Group 5: 463.1 N, and Group 6: 297.9 N. A statistically significant difference was found between Group 1 and Group 6 (p< 0.01). Conclusion It was concluded different ferrule designs did not have any influence on the fracture resistance of teeth with fiber posts. The results of this study indicate fiber posts can safely be used for their reinforcing properties. Furthermore, there is no significant change in the resistance of teeth with fiber posts regardless of which ferrule design is incorporated. The property of these types of posts is an additional advantage in clinical practice. Citation Dikbas I, Tanalp J, Ozel E, Koksal T, Ersoy M. Evaluation of the Effect of Different Ferrule Designs on the Fracture Resistance of Endodontically Treated Maxillary Central Incisors Incorporating Fiber Posts, Composite Cores and Crown Restorations. J Contemp Dent Pract 2007 November; (8)7:062-069.

scholarly journals Correlation between Intraocular Pressure and Thickness of Extraocular Muscles, the Severity and Activity of Thyroid-associated Orbitopathy

Folia Medica ◽  
2019 ◽  
Vol 61 (1) ◽  
pp. 90-96
Author(s):  
Nina Staneva Stoyanova ◽  
Marieta Konareva-Kostianeva ◽  
Vesela Mitkova-Hristova ◽  
Irina Angelova
Keyword(s):  
Intraocular Pressure ◽  
Extraocular Muscles ◽  
Ocular Motility ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Severity Of The Disease ◽  
Group 1

Abstract Aim: To establish the correlation between intraocular pressure (IOP) and thickness of extraocular muscles (EOM), the severity and activity of thyroid-associated orbitopathy (TAO). Materials and methods: 50 patients with TAO were included in the study. They all underwent a thorough ophthalmic examination, Goldmann tonometry (mm Hg) and computer tomography of the orbits, the muscle thickness sum (MTS) of each eye being measured in millimeters. According to the activity of TAO, the patients were divided into two groups – with and without activity, and according to the severity of the disease – into 6 groups: Group 1 (‘O’) had only subjective symptoms, group 2 (‘S’) had soft tissue symptoms, group 3 (‘P’) – proptosis, group 4 (‘E’) – ocular motility disorders, group 5 (‘C’) – corneal damage, and group 6 (‘Si’) – visual impairment; Results: TAO activity was registered in 21 patients (42 eyes, 42%) with MTS 23.39±3.81 and IOP 18.43±4.16. Twenty-nine patients (58 eyes, 58%) with MTS 19±3.21 and IOP 15.98±4.59 showed no TAO activity. The IOP within the groups, in terms of severity, was as follows: group 1 – 19.92+4.05; group 2 – 14.5±2.55; group 3 – 18.04±4.51; group 4 – 18.2±5.05; group 5 – 20.5±4.5; group 6 – 21.5±4.95. A correlation between the IOP and MTS was found. Conclusions: The IOP in patients with TAO depends on the thickness of the EOM, as well as on the activity and severity of the disease.

Newly developed dilute Russell’s viper venom reagents for lupus anticoagulant detection with improved specificity

Lupus ◽  
2017 ◽  
Vol 27 (1) ◽  
pp. 95-104 ◽  
Author(s):  
G W Moore ◽  
M Peyrafitte ◽  
C Dunois ◽  
J Amiral
Keyword(s):  
Gold Standard ◽  
Lupus Anticoagulant ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Russell’S Viper ◽  
Group 5 ◽  
Group 2 ◽  
Improved Accuracy ◽  
Group 1

Background Dilute Russell’s viper venom time (dRVVT) is indispensible in lupus anticoagulant (LA) detection yet commercial reagents from different suppliers perform variably, no gold standard assays exist and therapeutic anticoagulation interference is problematic. Objective The objective of this study was to compare a new formulation dRVVT with two currently available dRVVTs. Materials and methods Life Diagnostics (LD) dRVVT and Stago PTT-LA were routinely used for lupus anticoagulant detection, plus Taipan snake venom time/ecarin time (TSVT/ET) for patients on warfarin or rivaroxaban. Siemens dRVVT and the new HYPHEN BioMed (HBM) dRVVT were tested with 193 patient samples. Group 1, 59 non-anticoagulated patients (NAPs) LA-positive in LD dRVVT; Group 2, 15 PTT-LA-positive/dRVVT-negative NAPs; Group 3, 24 LA-positive warfarinized patients; Group 4, 13 patients on rivaroxaban; Group 5, 62 LA-negative thrombotic NAPs; Group 6, 20 warfarinized, non-antiphospholipid syndrome patients. Results Accepting that the Life Diagnostics reagents were acting as a pseudo-gold standard, Siemens dRVVT detected 56/59, (95%) Group 1 LA and HBM dRVVT 46/59, (76%), one each from Group 2, and Siemens dRVVT detected one in Group 5. The lower HBM dRVVT detection rate mainly concerned weaker LA, where between-reagent concordance is problematic. All Group 3 patients appeared LA-positive in undiluted plasma with Siemens dRVVT, as did 16/24 (67%) with HBM dRVVT but the fewer LA-positives in mixing tests better mapped to clear LA-positives with LD dRVVT. LD and Siemens dRVVTs exhibited 87% and 95% false-positivity for Group 6 whilst HBM dRVVT had none. Increasing the cut-off improved accuracy. Applying higher cut-offs improved accuracy in Group 4 patients. Conclusion HBM dRVVT exhibited improved specificity, mainly due to less interference by anticoagulation, but reduced sensitivity, compared to the other dRVVTs employed.

scholarly journals Standardised Models for Inducing Experimental Peritoneal Adhesions in Female Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Bernhard Kraemer ◽  
Christian Wallwiener ◽  
Taufiek K. Rajab ◽  
Christoph Brochhausen ◽  
Markus Wallwiener ◽  
...  
Keyword(s):  
Parietal Peritoneum ◽  
Female Rats ◽  
Peritoneal Adhesions ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Animal models for adhesion induction are heterogeneous and often poorly described. We compare and discuss different models to induce peritoneal adhesions in a randomized, experimental in vivo animal study with 72 female Wistar rats. Six different standardized techniques for peritoneal trauma were used: brushing of peritoneal sidewall and uterine horns (group 1), brushing of parietal peritoneum only (group 2), sharp excision of parietal peritoneum closed with interrupted sutures (group 3), ischemic buttons by grasping the parietal peritoneum and ligating the base with Vicryl suture (group 4), bipolar electrocoagulation of the peritoneum (group 5), and traumatisation by electrocoagulation followed by closure of the resulting peritoneal defect using Vicryl sutures (group 6). Upon second look, there were significant differences in the adhesion incidence between the groups (P<0.01). Analysis of the fraction of adhesions showed that groups 2 (0%) and 5 (4%) were significantly less than the other groups (P<0.01). Furthermore, group 6 (69%) was significantly higher than group 1 (48%) (P<0.05) and group 4 (47%) (P<0.05). There was no difference between group 3 (60%) and group 6 (P=0.2). From a clinical viewpoint, comparison of different electrocoagulation modes and pharmaceutical adhesion barriers is possible with standardised models.

Clinical Phenotype and Response to Imatinib of Chronic Myelogenous Leukemia Patients Harbouring Atypical BCR-ABL Transcripts. A Retrospective Analysis From the French Group of CML (Fi-LMC) and the French Group of Molecular Biologists for Hematological Malignancies (GBMHM).

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 3378-3378
Author(s):  
Benedicte Deau ◽  
Mathias Montveneur ◽  
Valérie Coiteux ◽  
Aline Renneville ◽  
Francoise Rigal-Huguet ◽  
...  
Keyword(s):  
Myelogenous Leukemia ◽  
Group 4 ◽  
Best Response ◽  
Group 6 ◽  
French Group ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Abstract Abstract 3378 Chronic Myelogenous Leukemia (CML) originates in the Philadelphia chromosome, a reciprocal translocation creating the fusion oncogene BCR-ABL. In 1–2% of CML cases, breakpoints fall outside the M-BCR gene on chromosome 22, leading to the synthesis of a variety of atypical BCR-ABL transcripts [shortened: e1a2 (m-BCR), e6a2, e8a2, b2a3 (e13a3), b3a3 (e14a3), or elongated transcripts: e19a2 (m-BCR)] and to the synthesis of different molecular weight BCR-ABL proteins that might have different tyrosine kinase activities. Thus, clinical phenotypes and BCR-ABL inhibition by tyrosine kinase inhibitors might be different and lead to different prognostic features. We retrospectively analysed at the national level, the clinical characteristics and the responses to imatinib (IM) of 63 patients with CML harbouring atypical BCR-ABL transcripts: 22 e1a2 [Group 1 (G1)], 20 e19a2 [Group 2 (G2)], 5 e8a2 [Group 3 (G3)], 4 e6a2 [Group 4 (G4)], 5 b2a3 [Group 5 (G5)], and 3 b3a3 [Group 6 (G6)] BCR-ABL transcripts. The general characteristics of the patients and their best response to IM are depicted in Table 1: Table 1 Group 1(e1a2) Group 2 (e19a2) Group 3 (e8a2) Group 4 (e6a2) Group 5 (b2a3) Group 6 (b3a3) n 22 20 5 8 5 3 M/F 7/15 6/14 4/1 4/4 5/0 0/3 Median age (years) 70 69 43 57 62 47 CP/AccP/MBC 20/0/2 17/1/2 5/0/0 4/1/3 4/1/0 2/1/0 Sokal (L/H/I/Ukn)* 6/8/2/4 1/3/9/4 3/1/0/1 1/2/1/0 1/2/0/1 0/2/0/0 Leukocytes (G/l, median) 60.85 28.3 55 28.4 93 82.4 Hemoglobin (g/dl, median) 12 10.2 11.7 10.95 11.1 10.2 Platelets (G/l, median) 303 848 253 259 167 363 Monocytes (G/l median) 4.8 0.8 2.34 0.05 1.08 0.825 Additional Clonal Abnormalities at diag (% of patients) 20 28 0 29 25 0 IM duration (median, years) 1.55 1.38 1.58 0.8 1.13 1.42 Interval Diagnosis-IM (median, years) 1.31 1.48 1 1.17 0.87 1.66 Best response to IM* No response 20 0 0 0 0 0 CHR (%) 13 32 0 0 0 0 Minor CyR (%) 47 0 0 0 0 0 PCyR (%) 0 10 20 10 25 67 CCyR (%) 13 32 60 50 0 0 MMR (%) 7 26 20 40 75 33 Follow-up since diag (median, years) 3.24 1.57 1.6 3.82 1.5 1.68 (CP states for Chronic phase, AccP for accelerated phase, MBC for myeloid blast crisis, L for Low, I for intermediate, H for High, Ukn for Unknown, * For CP patients only) Surprisingly, e1a2 and e19a2 transcripts seem significantly more frequent in females than in males conversely to typical BCR-ABL transcripts (p=0.01) and occurring more often in the elderly (p=0.05). The majority of the patients presented with typical cytological CML features, however, a significant monocytosis was observed in e1a2 and e8a2 atypical transcripts (p=0.0002). The median time on IM and the interval between diagnosis and IM were not statistically different between the 6 groups. Overall, there was no significant difference in the (hematologic, cytogenetic, molecular) responses to IM, but e1a2 transcripts seem less sensitive to this agent. The overall survival since diagnosis or since IM initiation was not different between atypical transcripts (p=0.55 and p=0.73 respectively), however, the progression-free survival (PFS) since diagnosis with e1a2 transcripts was significantly worse than for all other atypical transcripts (p=0.02) as shown in Figure 1: The PFS since IM initiation was somewhat worse for e1a2 transcripts, but close to significance (p=0.09), but the follow-up is not very long yet. Fifteen patients among 63 had second generation TKIs (TKI2), 7 in group 1, 3 in group 2, 1 in groups 3, 4, 5, and 2 in group 6. Only one patient (b3a3 transcript) developed a MBC being on IM. Two patients developed a T315I BCR-ABL mutation (1 e1a2, and 1 e6a2). Two patients got allo-transplanted (1 e1a2 alive and well at last follow-up, 1 e19 a2 died from GVHD). In conclusion, atypical BCR-ABL transcripts induce a particular molecular and subsequent clinical phenotypes, particularly e1a2 transcripts showing in this study poor prognosis features. The response of atypical BCR-ABL transcripts to IM might vary from that what it is for classical M-BCR transcripts, but a longer follow-up is needed. Disclosures: No relevant conflicts of interest to declare.

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