scholarly journals An Immuno-Histochemical Assessment of Ki67, P53 Over-Expression in Helicobacter Pylori Positive Gastritis.

2021 ◽  
Author(s):  
Karma Turath Tawfeeq ◽  
Elaf Abdulwahhab Hamdi ◽  
Nadwa Subhi Al-azzo
Keyword(s):  
Helicobacter Pylori ◽  
P53 Protein ◽  
Common Disease ◽  
Chronic Inflammatory Cell ◽  
Ki 67 ◽  
P53 Expression ◽  
Significance Level ◽  
Over Expression ◽  
Study Results ◽  
Sydney System

Abstract Background and Objective: Helicobacter pylori infection of the stomach is a common disease and the resulting changes from it are numerous and deserve to be in the focus of researchers' attention. The aim of this study is to assess the expression of mutant P53 protein and Ki-67 markers in patients with gastritis secondary to Helicobacter pylori.Methods: Thirty samples with positive Helicobacter pylori gastritis were included in a retrospective study in Mosul / Iraq. The histological parameters were assessed using the Sydney system, then the expression of Ki67 and P53 expression were studied by immunohistochemical methods. The significance level was appointed at (0.05).Results: Ki67 and P53 expression were found in 83.3% of the total cases. The study results show that 92% of positive Ki67, P53 cases had chronic inflammatory cell infiltration, polymorph nuclear cells infiltration, and atrophy. Whereas 96% of positive Ki67 cases had no metaplasia, 92% of the positive P53 cases had no metaplasia. The results also showed that only 16% of the positive Ki67 cases had dysplastic changes, and 24 % of the positive cases of P53 cases were showed dysplasia. moreover, whenever P53 was negative; there is neither metaplasia nor dysplasia in the tissue, this does not apply to Ki67 negative cases.Conclusions: Ki67, P53 expressions increase with chronicity of Helicobacter pylori-positive gastritis, P53 expression is amplified when atrophy is present in these samples

Immunohistochemical study of p53 expression in endometrial carcinomas: correlation with markers of proliferating cells and clinicopathologic features

1993 ◽  
Vol 3 (6) ◽  
pp. 363-368 ◽  
Author(s):  
T. Hachisuga ◽  
K. Fukuda ◽  
M. Uchiyama ◽  
N. Matsuo ◽  
T. Iwasaka ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferative Activity ◽  
P53 Protein ◽  
Myometrial Invasion ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Normal Endometrium ◽  
P53 Expression ◽  
Dna Polymerase Α ◽  
Endometrial Carcinomas

Using anti-p53 (PAb1801 and PAb240), anti-DNA polymerase α and Ki-67 monoclonal antibodies, the expression of p53 was studied in 11 normal endometria, 14 endometrial hyperplasias and 27 endometrial carcinomas and its relationship to the proliferative activity of the tumors was examined. Normal endometria and simple hyperplasias were completely negative for p53. The PAb1801 indices of complex hyperplasias and complex atypical hyperplasias were 2.5±1.8% and 5.0±3.2%, respectively. The PAb1801 indices of grade 1, grade 2 and grade 3 endometrial carcinomas were 10.2±14.2%, 44.4±29/0% and 45.0±32.5%, respectively. These results indicate a progressively enhanced p53 expression in the sequence from normal endometrium, through hyperplasia to carcinoma. A significant correlation between p53 expression and labeling indices of Ki-67 and DNA polymerase α was observed in endometrial carcinomas. The endo-metrial carcinomas with p53 overexpression developed mainly in post-menopausal patients and were frequently high-grade tumors with deep myometrial invasion. These findings may indicate that overexpression of p53 protein contributes to the proliferative activity of the tumor cells.

Igf-Ii Mrna Expression in Breast Cancer: Predictive Value and Relationship to Other Prognostic Factors

2010 ◽  
Vol 25 (3) ◽  
pp. 150-156 ◽  
Author(s):  
Emilio Fiore ◽  
Daniela Campani ◽  
Ilaria Muller ◽  
Valentina Belardi ◽  
Elisa Giustarini ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Mrna Expression ◽  
Ductal Carcinoma ◽  
P53 Protein ◽  
Independent Predictive Factor ◽  
Ki 67 ◽  
P53 Expression ◽  
Predictive Parameters ◽  
The Difference

Purpose Insulin-like growth factor-II (IGF-II) is an important regulator of tumor growth in breast cancer. In this study we have examined the prognostic value of IGF-II mRNA expression in breast cancer and its relationship to other predictive parameters. Patients Sixty-eight women with infiltrating ductal carcinoma were given the same treatments including mastectomy and antitumoral therapies and followed up for 5 years. Results The overall 5-year survival rate was 73.5% (55/68). IGF-II mRNA was expressed in 33/64 patients (51.6%) and had no significant impact on survival. The expression of estrogen receptor (ER) and progesterone receptor (PgR) did not significantly affect the 5-year survival, but in the presence of an IGF-II mRNA signal, the survival of ER- and PgR-negative patients (n=9) was lower than that of ER- and PgR-positive patients (n=15), although the difference was not significant. The 5-year survival was not significantly different between Ki-67-positive and negative patients, but in the IGF-II positive group Ki-67-positive patients (n=7) had a significantly poorer prognosis than Ki-67-negative patients (n=26). The expression of p53 protein was associated with a poorer prognosis: 6/11 (54.5%) p53-positive patients died in the first 26 months of follow-up and 5 of these 6 patients (83.3%) also had positive IGF-II mRNA expression. Conclusions IGF-II mRNA expression per se is not an independent predictive factor in breast cancer but may be a marker of poor prognosis when associated with other prognostic factors such as Ki-67 index and p53 expression.

Assessment of p21, p53 expression, and Ki-67 proliferative activities in the gastric mucosa of children with Helicobacter pylori gastritis

2015 ◽  
Vol 27 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Coskun Saf ◽  
Enver Mahir Gulcan ◽  
Ferda Ozkan ◽  
Seyhan Perihan Cobanoglu Saf ◽  
Ayca Vitrinel
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Ki 67 ◽  
P53 Expression ◽  
Helicobacter Pylori Gastritis

scholarly journals A study on the effect of Helicobacter pylori infection on p53 expression in gastric cancer and gastritis tissues

2013 ◽  
Vol 7 (09) ◽  
pp. 651-657 ◽  
Author(s):  
Barik A Salih ◽  
Zuhal Gucin ◽  
Nizamettin Bayyurt
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
P53 Protein ◽  
Immunohistochemical Analysis ◽  
Intestinal Type ◽  
Diffuse Type ◽  
P53 Expression ◽  
Normal Tissues ◽  
Genotype Frequencies ◽  
H Pylori

Introduction: Helicobacter pylori cause damage to gastric epithelial cells and alterations in the p53 gene that lead to cancer development. This study aimed to determine the correlation of p53 expression with H. pylori using immunohistochemistry, RFLP-PCR, and histopathology. Methodology: Gastric biopsy samples from gastric cancer (GC) (n = 54) and gastritis (n = 31) patients were examined for histopathological changes and expression of p53 protein by immunohistochemistry. Results: Immunohistochemical analysis of p53 protein expression in H. pylori-positive GC sections showed an average of 44.3% positive cells in tumors and 6.9% in normal tissues, as compared to 16.4% and 4.4% in H. pylori-negative sections. P53 expression showed significant association with H. pylori (P = 0.005), invasion depth (P = 0.029) and inflammation reaction (P = 0.008). In gastritis sections, no difference in the average p53 staining in H. pylori-positive or -negative sections was seen. PCR-RFLP results also showed no difference in genotype frequencies of p53 in H. pylori-positive or -negative gastritis sections. Histopathology study of H. pylori-positive GC sections showed that 97.2% were the intestinal type and 2.8% the diffuse type, while in H. pylori-negative sections 35.2% were the intestinal type and 64.8% the diffuse type. Biopsy sections from H. pylori-positive gastritis patients revealed more severe inflammation than those of H. pylori-negative patients. Conclusion: Our results show that H. pylori infection affects p53 expression in GC. The average p53 expression was significantly higher in tumor than in normal tissues. In gastritis sections p53 expression was significantly associated with H. pylori.

scholarly journals Immunohistochemical study of P53 protein expression in different prostate cancer Gleason grading groups

2020 ◽  
Vol 24 (2) ◽  
pp. 145-155 ◽  
Author(s):  
G. Y. Kudryavtsev ◽  
L. V. Kudryavtseva ◽  
L. M. Mikhaleva ◽  
Y. Y. Kudryavtseva ◽  
N. A. Solovyeva ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Proliferative Activity ◽  
Immunohistochemical Study ◽  
P53 Protein ◽  
Public Health Problem ◽  
Developed Countries ◽  
Ki 67 ◽  
P53 Expression ◽  
Gleason Grading ◽  
The Difference

Prostate cancer (PC) remains an urgent public health problem, especially in developed countries. The use of immunohistochemical research methods in addition to the morphological classification of prostate adenocarcinomas allows a more accurate diagnosis and prognosis of the disease. The aim of the study is to identify isoforms of P53 using clones of mouse antibodies (D-07 and Y5; Epitomics, USA) in prostate cancer with different proliferative activity and the degree of malignancy. Materials and Methods: The work included surgical material for prostate resection and prostatectomy, as well as biopsy specimens (56 cases in total). An immunohistochemical study was carried out with the Ki-67 marker, as well as with mouse monoclonal antibodies (D-07 and Y5) to the P53 protein, interacting with its wild and mutant isoforms. The significance of the difference in the samples was determined using the Mann-Whitney U-test, correlation relationships were determined using the Spearman coefficient. Results: Expression of P53 upon interaction with antibodies D-07 and Y5 was determined in 56.3% and 39.6%, respectively. A statistically significant direct correlation was found between the severity of P53 expression when interacting with Y5 antibodies and the degree of tumor differentiation (rs = 0.567, p 0.05), as well as between the expression level of this protein and tumor proliferative activity (rs = 0.698, p 0.05). Conclusion: Antibodies of clone D-07, interacting with both wild and mutant isoforms of P53 protein, show positive expression in adenocarcinomas of all degrees. Expression of the mutant P53 protein is most pronounced in low-differentiated carcinomas and correlates with high proliferative activity of tumor cells, which may be associated with a loss in the induction of P53-dependent apoptosis.

scholarly journals Heterogeneity of Ki-67 and p53 Expression in Glioblastoma

2014 ◽  
Vol 14 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Arvids Jakovlevs ◽  
Andrejs Vanags ◽  
Dainis Balodis ◽  
Janis Gardovskis ◽  
Ilze Strumfa
Keyword(s):  
Protein Expression ◽  
Diagnostic Accuracy ◽  
Cellular Proliferation ◽  
P53 Protein ◽  
World Health ◽  
Residual Tumour ◽  
Ki 67 ◽  
P53 Expression ◽  
Proliferation Activity ◽  
The Mean

Summary Introduction. Heterogeneity is a characteristic feature of malignant tumours. It challenges the treatment regimens as well as can impair the diagnostic accuracy. Glioblastoma multiforme (GBM), a high-grade malignant glial tumour, is known for the extreme morphological heterogeneity giving rise also to the term itself. Aim of the study was to evaluate heterogeneity of pathogenetically and diagnostically important cardinal tumour features, namely, cellular proliferation and tumour suppressor protein expression in GBMs. Material and methods. The study group comprised 101 GBMs, retrospectively identified by archive search. The inclusion criteria comprised validated diagnosis (by World Health Organisation criteria) and lack of prior treatment. Recurrent GBMs as well as other glial and non-glial tumours were excluded from the study. Insufficient tissue materials comprising stereotactic biopsies and tissues affected by widespread necrosis (exceeding 90%) were also excluded. Proliferation activity (by Ki-67) and expression of aberrant p53 protein was detected by immunohistochemical investigation (IHC) of formalin-fixed, paraplast-embedded tumour samples. Polymeric visualisation system was used to detect bound primary antibodies. The expression of each antigen was measured by computed morphometry in at least 200 cells of hot and cold spots in each tumour. The data were expressed as the relative value. Heterogeneity was estimated as the mathematical difference between the highest and lowest expression value in each tumour. Descriptive statistics was applied. The 95% confidence intervals (CI) were determined as well. Results. The highest proliferation activity ranged 15 – 95%; mean 43.9% [95% CI = 40.3 – 47.6]. The lowest proliferation activity ranged 2 – 95%, mean 20.1% [16.8 – 23.4]. The mean proliferation heterogeneity was 23.8% [21.5 – 26.2]; range 0 – 67%. The mean heterogeneity of p53 protein expression was 11.7% [8.9 – 14.6], ranging 0 – 75%. Conclusions. GBM is characterized by marked heterogeneity regarding proliferation rate and expression of p53 protein that may affect diagnostic accuracy and grading of gliomas in small samples of tissue material as well as survival in case of small residual tumour after surgical treatment.

scholarly journals Correlation between mast cell density and histological parameters in Helicobacter pylori-associated gastritis

2011 ◽  
Vol 5 (1) ◽  
pp. 163-167 ◽  
Author(s):  
Mana Taweevisit ◽  
Naruemon Klaikaew
Keyword(s):  
Helicobacter Pylori ◽  
Mast Cell ◽  
Mast Cells ◽  
Cell Density ◽  
B Cell Lymphoma ◽  
Inflammatory Cells ◽  
Chronic Inflammatory Cell ◽  
Mast Cell Density ◽  
Sydney System ◽  
H Pylori

Abstract Background: Helicobacter pylori (H. pylori) are a major cause of chronic gastritis and peptic ulcer. This organism plays a role in gastric carcinoma and B-cell lymphoma. However, the exact pathogenesis of gastric inflammation is still unclear. Mast cells, the important inflammatory cells for allergic process, may participate in the pathogenesis of gastritis related to H. pylori infection. Objective: Analyze the relationship between mast cell density, H. pylori intensity, histological alterations, and their severity of biopsy proven gastritis. Methods: One hundred eleven biopsied specimens were collected from Thai patients who were diagnosed H. pylori-associated gastritis of the antrum at King Chulalongkorn Memorial Hospital between 2002 and 2005. All biopsied specimens were examined according to the Updated Sydney System. Mast cell density was evaluated by 0.1% toluidine-stained sections. Results: The higher mast cell density was correlated with increased neutrophilic infiltration (r = 0.220, p = 0.020), chronic inflammatory cell infiltration (r = 0.381, p <0.001), and lymphoid aggregation (r = 0.271, p = 0.004). No relationship was found between mast cell density and intensity of H. pylori, glandular atrophy, or intestinal metaplasia. Conclusion: Mast cells might take part in the pathogenesis of H. pylori gastritis.

scholarly journals The expression and significance of p53 protein and Ki-67 protein in pterygium

2016 ◽  
Vol 73 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Vesna Ljubojevic ◽  
Radoslav Gajanin ◽  
Ljiljana Amidzic ◽  
Zoran Vujkovic
Keyword(s):  
P53 Protein ◽  
Tissue Growth ◽  
Ki 67 ◽  
P53 Expression ◽  
Factors Affecting ◽  
Gender And Age ◽  
Study Support ◽  
Positive Correlations ◽  
The Relationship ◽  
Growth Disorder

Background/Aim. Pterygium is considered to be a degenerative disease of the conjunctiva, however, the presence of tumor markers in pterygium reinforces the hypothesis that this lesion is similar to tumor. Inactivation of p53 function removes an obstacle to increased proliferation. Factors affecting the prevalence of p53 expression in pterygium deserve investigation. The aim of the study was to investigate the expression of p53 and Ki-67 proteins in pterygium and normal conjunctiva, the effects of gender and age on p53 expression, and the relationship between the expression of p53 and Ki-67 proteins. Methods. A total of 34 samples of pterygium and 34 samples of the normal conjunctiva were analyzed. The samples were studied by immunohistochemistry using antibodies against p53 and Ki-67. Results. Totally 15 (44%) samples of pterygia were p53 positive. Correlations between the expression of p53 protein and sex, and age were not established. The number of Ki-67 positive cells in pterygium (9.74%) was significantly higher than the number of Ki-67 positive cells in the normal conjunctiva (1.74%), (p = 0.001). Between the expression of p53 protein and Ki-67 protein in pterygium there was a significant positive correlation (p = 0.000). Conclusion. The prevalence of p53 positive samples of pterygium was 44%. The influence of sex and age on p53 protein expression in pterygium was not found. The increased proliferative acivity was present in the epithelium of pterygium. The expression of Ki-67 protein is associated with the expression of p53 protein in pterygium. The findings of our study support the thesis of pterygium as tissue growth disorder.

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