scholarly journals PAHs Changed Epigenetics by One Carbon Metabolism in Childhood Asthma

2021 ◽  
Author(s):  
Hao Wu ◽  
Yuling Bao ◽  
Jinye Hu ◽  
Hui Huang ◽  
Ping Jiang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Histone Methylation ◽  
Carbon Metabolism ◽  
Childhood Asthma ◽  
Promoter Region ◽  
Total Serum ◽  
Serum Ige ◽  
Asthmatic Children ◽  
One Carbon Metabolism ◽  
Asthmatic Group

Abstract Background: Epigenetic mechanisms may play a role in which PAHs exert its adverse effects in childhood asthma. However, the underlying molecular mechanism remain to be fully elucidated. This study aimed to investigate this process in view of cellular metabolism, especially one carbon metabolism. Methods: Fifty asthmatic children and fifty control subjects were recruited in this study. Serum IgE and IL-17A was detected by ELISA assay. Serum PAHs levels were measured by GC-MS. One carbon-related metabolites were determined by UPLC-Orbitrap-MS. Blood DNA methylation in long interspersed nucleotide element-1 (LINE-1) was analyzed by bisulfite sequencing PCR. ChIP assays were used to examine H3K4me3 modifications on IL-17A gene. Multivariable linear regression was performed to evaluate the associates between PAHs and DNA methylation and histone methylation mediated by one carbon metabolism. Results: The asthmatic group presented significantly higher total serum IgE and IL-17A levels. Serum Fla was associated with childhood asthma. The asthmatic group displayed a significantly decreasing in SAM abundance and a smaller but corresponding decrease in SAH, which indicated the increasing conversion from SAM to SAH and the elevated capacity of methylation reactions. Fla had a great effect on one carbon metabolites, especially SAH, SAM and Ser, which exerted significant mediation effects between the Fla level and asthma. What’s more, Fla had a positive effect on LINE-1 DNA methylation (β=0.395, P=0.000) and H3K4 tri-methylation level in the IL-17A promoter region(β=0.293, P=0.002). We did find significant mediation effect between serum Fla and asthma by LINE-1 DNA methylation and H3K4me3 level in the IL-17A promoter region.Conclusion: PAHs disturbed one carbon metabolism to influence the methyl group refill of DNA methylation and histone methylation, which may elevate serum IL-17A level in asthmatic children.

Age-dependent relationship between bronchial hyperresponsiveness to methacholine and total serum IgE level in asthmatic children

2001 ◽  
Vol 87 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Miyuki Kono ◽  
Hiroyuki Mochizuki ◽  
Hirokazu Arakawa ◽  
Masahiko Kato ◽  
Kenichi Tokuyama ◽  
...  
Keyword(s):  
Bronchial Hyperresponsiveness ◽  
Total Serum ◽  
Serum Ige ◽  
Asthmatic Children ◽  
Dependent Relationship ◽  
Age Dependent

Metformin regulates global DNA methylation via mitochondrial one-carbon metabolism

Oncogene ◽  
2017 ◽  
Vol 37 (7) ◽  
pp. 963-970 ◽  
Author(s):  
E Cuyàs ◽  
S Fernández-Arroyo ◽  
S Verdura ◽  
R Á-F García ◽  
J Stursa ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Carbon Metabolism ◽  
Global Dna Methylation ◽  
One Carbon Metabolism

scholarly journals Polyamine Metabolism and Gene Methylation in Conjunction with One-Carbon Metabolism

2018 ◽  
Vol 19 (10) ◽  
pp. 3106 ◽  
Author(s):  
Kuniyasu Soda
Keyword(s):  
Dna Methylation ◽  
Carbon Metabolism ◽  
Methylation Status ◽  
Significant Risk ◽  
Significant Risk Factor ◽  
Dna Methyltransferases ◽  
Polyamine Metabolism ◽  
Methyl Group ◽  
Wide Range ◽  
One Carbon Metabolism

Recent investigations have revealed that changes in DNA methylation status play an important role in aging-associated pathologies and lifespan. The methylation of DNA is regulated by DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) in the presence of S-adenosylmethionine (SAM), which serves as a methyl group donor. Increased availability of SAM enhances DNMT activity, while its metabolites, S-adenosyl-l-homocysteine (SAH) and decarboxylated S-adenosylmethionine (dcSAM), act to inhibit DNMT activity. SAH, which is converted from SAM by adding a methyl group to cytosine residues in DNA, is an intermediate precursor of homocysteine. dcSAM, converted from SAM by the enzymatic activity of adenosylmethionine decarboxylase, provides an aminopropyl group to synthesize the polyamines spermine and spermidine. Increased homocysteine levels are a significant risk factor for the development of a wide range of conditions, including cardiovascular diseases. However, successful homocysteine-lowering treatment by vitamins (B6, B12, and folate) failed to improve these conditions. Long-term increased polyamine intake elevated blood spermine levels and inhibited aging-associated pathologies in mice and humans. Spermine reversed changes (increased dcSAM, decreased DNMT activity, aberrant DNA methylation, and proinflammatory status) induced by the inhibition of ornithine decarboxylase. The relation between polyamine metabolism, one-carbon metabolism, DNA methylation, and the biological mechanism of spermine-induced lifespan extension is discussed.

scholarly journals Association between one-carbon metabolism indices and DNA methylation status in maternal and cord blood

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Anna K. Knight ◽  
Hea Jin Park ◽  
Dorothy B. Hausman ◽  
Jennifer M. Fleming ◽  
Victoria L. Bland ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cord Blood ◽  
Carbon Metabolism ◽  
Methylation Status ◽  
One Carbon Metabolism

Modeling folate, one-carbon metabolism, and DNA methylation

2008 ◽  
Vol 66 ◽  
pp. S27-S30 ◽  
Author(s):  
Cornelia M Ulrich ◽  
Michael C Reed ◽  
H Frederik Nijhout
Keyword(s):  
Dna Methylation ◽  
Carbon Metabolism ◽  
One Carbon Metabolism

scholarly journals Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation

Cell Reports ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2864-2873.e7 ◽  
Author(s):  
Mohamad-Ali Fawal ◽  
Thomas Jungas ◽  
Anthony Kischel ◽  
Christophe Audouard ◽  
Jason S. Iacovoni ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Neural Stem Cell ◽  
Cross Talk ◽  
Histone Methylation ◽  
Carbon Metabolism ◽  
Stem Cell Differentiation ◽  
One Carbon Metabolism

scholarly journals Domestic Exposure to Fungi and Total Serum IgE Levels in Asthmatic Children

2005 ◽  
Vol 2005 (3) ◽  
pp. 167-170 ◽  
Author(s):  
Huey-Jen Jenny Su ◽  
Pei-Chih Wu ◽  
Huan-Yao Lei ◽  
Jiu-Yao Wang
Keyword(s):  
Airborne Fungi ◽  
Total Serum ◽  
Dose Response Relationship ◽  
Total Ige ◽  
Serum Ige ◽  
Asthmatic Children ◽  
Specific Serum ◽  
Dust Mite ◽  
Der P1 ◽  
Dust Mite Allergen

We measured the number of airborne, viable fungi and house dust mite (HDM) allergen levels in the homes of a group of asthmatic children. Blood samples were drawn and the amounts of total and specific serum IgE were determined. The association between the number of fungal colonies, dust mite allergen exposure, and specific and total IgE was evaluated. The number of viable airborne fungi was high (20 543 CFU/m) in those investigated houses.Der p1concentrations on child's mattress exceeding 2μg/g were found in 78.6% of the houses. A quantitative dose-response relationship was demonstrated between the exposure to viable, airborne molds and the amount of total IgE (r=0.4399andP=.0249) and the level was further increased in children with coexposure to viable fungi and HDM.

scholarly journals Genetic polymorphisms relevant for one-carbon metabolism show no effect on homocysteine plasma levels and DNA methylation in alcoholism

2009 ◽  
Vol 19 (4) ◽  
pp. 215-216 ◽  
Author(s):  
Bernd Lenz ◽  
Carmen Soehngen ◽  
Michael Linnebank ◽  
Annemarie Heberlein ◽  
Helge Frieling ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Genetic Polymorphisms ◽  
Carbon Metabolism ◽  
Plasma Levels ◽  
One Carbon Metabolism

scholarly journals Study on the Relationship Between Folate, Dna Methylation and Esophagus Cancer

2017 ◽  
Vol 1 (3) ◽  
Author(s):  
Huang Guiling
Keyword(s):  
Dna Methylation ◽  
Carbon Metabolism ◽  
Early Screening ◽  
Methyl Group ◽  
Esophagus Cancer ◽  
Multiple Factors ◽  
Chinese Characteristic ◽  
The World ◽  
One Carbon Metabolism ◽  
The Relationship

Esophagus cancer is a common malignant tumor in China, and itsprevalence rate and fatality rate are the highest in the world whoseoccurrence is related to multiple factors, including family heredity,race, gender, region, diet and so on. Esophagus cancer is the diseasewith the most Chinese characteristic. The pathogenesis of esophagus cancer is very complicated, and the epigenetics in the pathogenetic process has become the current research hotspot, and the reversible feature provides a new direction for the early screening, prevention and treatment of esophagus cancer. DNA methylation is the most deep epigenetics in the current study, and the pattern of genome methylation is often abnormal in the cancerization of esophagus cancer. The folate, as the methyl group donor, and B-vitamins related to one-carbon metabolism, shall directly influence the condition of DNA methylation, give rise to the change of epigenetics, affect the occurrence of esophagus cancer. This paper summarizes the relationship among the occurrence of esophagus cancer and folate, several kinds of B-vitamins related to one-carbon metabolism, DNA methylation.

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